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RapB Regulates Cell Adhesion and Migration in Dictyostelium, Similar to RapA.
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RapB Regulates Cell Adhesion and Migration in Dictyostelium, Similar to RapA.
Uri Han1, Nara Han1, Byeonggyu Park1, Taeck Joong Jeon1,2
Journal of Microbiology 2024;62(8):627-637
DOI: https://doi.org/10.1007/s12275-024-00143-y
Published online: June 17, 2024
1Department of Integrative Biological Sciences and BK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Chosun University, Gwangju, 61452, Republic of Korea.
3The Basic Science Institute of Chosun University, Chosun University, Gwangju, 61452, Republic of Korea.
Corresponding author:  Taeck Joong Jeon,
Email: tjeon@chosun.ac.kr
Received: 12 March 2024   • Revised: 22 April 2024   • Accepted: 3 May 2024
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Ras small GTPases act as molecular switches in various cellular signaling pathways, including cell migration, proliferation, and differentiation. Three Rap proteins are present in Dictyostelium; RapA, RapB, and RapC. RapA and RapC have been reported to have opposing functions in the control of cell adhesion and migration. Here, we investigated the role of RapB, a member of the Ras GTPase subfamily in Dictyostelium, focusing on its involvement in cell adhesion, migration, and developmental processes. This study revealed that RapB, similar to RapA, played a crucial role in regulating cell morphology, adhesion, and migration. rapB null cells, which were generated by CRISPR/Cas9 gene editing, displayed altered cell size, reduced cell-substrate adhesion, and increased migration speed during chemotaxis. These phenotypes of rapB null cells were restored by the expression of RapB and RapA, but not RapC. Consistent with these results, RapB, similar to RapA, failed to rescue the phenotypes of rapC null cells, spread morphology, increased cell adhesion, and decreased migration speed during chemotaxis. Multicellular development of rapB null cells remained unaffected. These results suggest that RapB is involved in controlling cell morphology and cell adhesion. Importantly, RapB appears to play an inhibitory role in regulating the migration speed during chemotaxis, possibly by controlling cell-substrate adhesion, resembling the functions of RapA. These findings contribute to the understanding of the functional relationships among Ras subfamily proteins.

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    RapB Regulates Cell Adhesion and Migration in Dictyostelium, Similar to RapA.
    J. Microbiol. 2024;62(8):627-637.   Published online June 17, 2024
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