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Non-Mitochondrial Aconitase-2 Mediates the Transcription of Nuclear-Encoded Electron Transport Chain Genes in Fission Yeast.
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Journal Article
Non-Mitochondrial Aconitase-2 Mediates the Transcription of Nuclear-Encoded Electron Transport Chain Genes in Fission Yeast.
Ho-Jung Kim1, Soo-Yeon Cho1, Soo-Jin Jung2,3, Yong-Jun Cho4, Jung-Hye Roe2, Kyoung-Dong Kim1
Journal of Microbiology 2024;62(8):639-648
DOI: https://doi.org/10.1007/s12275-024-00147-8
Published online: June 25, 2024
1Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, Republic of Korea.
2School of Biological Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
3Center for RNA Research, Institute for Basic Science, Seoul, 08826, Republic of Korea.
4Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chuncheon, 24341, Republic of Korea.
Corresponding author:  Kyoung-Dong Kim,
Email: kdkim0122@cau.ac.kr
Received: 27 March 2024   • Revised: 6 May 2024   • Accepted: 7 May 2024
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Aconitase-2 (Aco2) is present in the mitochondria, cytosol, and nucleus of fission yeast. To explore its function beyond the well-known role in the mitochondrial tricarboxylic acid (TCA) cycle, we conducted genome-wide profiling using the aco2ΔNLS mutant, which lacks a nuclear localization signal (NLS). The RNA sequencing (RNA-seq) data showed a general downregulation of electron transport chain (ETC) genes in the aco2ΔNLS mutant, except for those in the complex II, leading to a growth defect in respiratory-prone media. Complementation analysis with non-catalytic Aco2 [aco2ΔNLS + aco2(3CS)], where three cysteines were substituted with serine, restored normal growth and typical ETC gene expression. This suggests that Aco2's catalytic activity is not essential for its role in ETC gene regulation. Our mRNA decay assay indicated that the decrease in ETC gene expression was due to transcriptional regulation rather than changes in mRNA stability. Additionally, we investigated the Php complex's role in ETC gene regulation and found that ETC genes, except those within complex II, were downregulated in php3Δ and php5Δ strains, similar to the aco2ΔNLS mutant. These findings highlight a novel role for nuclear aconitase in ETC gene regulation and suggest a potential connection between the Php complex and Aco2.

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    Non-Mitochondrial Aconitase-2 Mediates the Transcription of Nuclear-Encoded Electron Transport Chain Genes in Fission Yeast.
    J. Microbiol. 2024;62(8):639-648.   Published online June 25, 2024
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