5’ Untranslated Region of the Pseudomonas putida WCS358 Stationary Phase Sigma Factor rpoS mRNA is Involved in RpoS Translational Regulation

Branko Jovcic; Iris Bertani; Vittorio Venturi; Ljubisa Topisirovic; Milan Kojic

doi:10.1007/s12275-007-0127-2

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5’ Untranslated Region of the Pseudomonas putida WCS358 Stationary Phase Sigma Factor rpoS mRNA is Involved in RpoS Translational Regulation: Branko Jovcic ¹, Iris Bertani ², Vittorio Venturi ², Ljubisa Topisirovic ¹, Milan Kojic ¹; Journal of Microbiology 2008;46(1):56-61.
DOI: https://doi.org/10.1007/s12275-007-0127-2

Author Affiliations

¹Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444/a, PO Box 23, 11010 Belgrade, Serbia, ²International Centre for Genetic Engineering and Biotechnology, Area Science Park, 34012 Trieste, Italy

Corresponding author: Milan Kojic , Tel: 381-11-3975-960,

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Abstract

The σS subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5’ untranslated region (5’ UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5’ UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5’ UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.

Keywords: Pseudomonas;rpoS;translational regulation

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5’ Untranslated Region of the Pseudomonas putida WCS358 Stationary Phase Sigma Factor rpoS mRNA is Involved in RpoS Translational Regulation

J. Microbiol. 2008;46(1):56-61.

DOI: https://doi.org/10.1007/s12275-007-0127-2

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