Denaturing Gradient Gel Electrophoresis Analysis of Bacterial Community Profiles in the Rhizosphere of cry1AC-carrying Brassica rapa subsp. pekinensis

Sera Jung; Semi Park; Daeha Kim; Seung Bum Kim

doi:10.1007/s12275-007-0190-8

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Denaturing Gradient Gel Electrophoresis Analysis of Bacterial Community Profiles in the Rhizosphere of cry1AC-carrying Brassica rapa subsp. pekinensis: Sera Jung ¹, Semi Park ², Daeha Kim ², Seung Bum Kim ²; Journal of Microbiology 2008;46(1):12-15.
DOI: https://doi.org/10.1007/s12275-007-0190-8

Author Affiliations

¹Graduate School of Public Health and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea, ²Department of Biological Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea¹Graduate School of Public Health and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea, ²Department of Biological Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea

Corresponding author: Seung Bum Kim , Tel: 82-42-821-6412,

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Abstract

The effect of genetically modified (GM) Brassica rapa subsp. pekinensis (Chinese cabbage) expressing Bt toxin gene (cry1AC) to the rhizosphere bacterial community was examined using the denaturing gradient gel electrophoresis (DGGE) fingerprinting method. From the visual comparison of the DGGE profiles, there were no significant differences between the profiles of Bt and control rhizosphere in both Suwon and Yesan samples. From the sequence analysis of the individual bands, Sphingomonas sp. of Alphaproteobacteria and several actinobacterial members were identified as the main bacterial taxa in both Suwon and Yesan samples. In the multiple correspondence analysis, no clear separation between Bt and control rhizosphere was seen in both Suwon and Yesan datasets. The profiles of bulk soils were separated from those of rhizosphere. The DGGE fingerprinting analyses indicated that Bt crops did not significantly alter the genetic composition of rhizosphere bacterial communities.

Keywords: cry1AC;rhizobacteria;Brassica rapa subsp. pekinensis;denaturing gradient gel electrophoresis

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Denaturing Gradient Gel Electrophoresis Analysis of Bacterial Community Profiles in the Rhizosphere of cry1AC-carrying Brassica rapa subsp. pekinensis

J. Microbiol. 2008;46(1):12-15.

DOI: https://doi.org/10.1007/s12275-007-0190-8

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