Mutant Selection of Hahella chejuensis KCTC 2396 and Statistical Optimization of Medium Components for Prodigiosin Yield-Up

Sung Jin Kim; Hong Kum Lee; Yoo Kyung Lee; Joung Han Yim

doi:10.1007/s12275-008-0037-y

Articles

Page Path: HOME > J. Microbiol > Volume 46(2); 2008 > Article

Research Support, Non-U.S. Gov't
Mutant Selection of Hahella chejuensis KCTC 2396 and Statistical Optimization of Medium Components for Prodigiosin Yield-Up: Sung Jin Kim ^1,2, Hong Kum Lee ¹, Yoo Kyung Lee ¹, Joung Han Yim ¹; Journal of Microbiology 2008;46(2):183-188.
DOI: https://doi.org/10.1007/s12275-008-0037-y
Published online: June 11, 2008

¹Polar BioCenter, Korea Polar Research Institute, KORDI, Incheon 406-840, Republic of Korea, ²Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea

Corresponding author: Joung Han Yim , Tel: 82-32-260-6340,

Received: 12 February 2008 • Accepted: 4 April 2008

prev next

110 Views
0 Download
20 Crossref
25 Scopus

Full Article

Abstract

Prodigiosin is a natural red pigment with algicidal activity against Cochlodinium polykrikoides, a major harmful red-tide microalga. To increase the yield of prodigiosin, a mutant of Hahella chejuenesis KCTC 2396, assigned M3349, was developed by an antibiotic mutagenesis using chloramphenicol. When cultured in Sucrose-based Marine Broth medium (SMB), M3349 could produce prodigiosin at 1.628±0.06 g/L, while wild type producing at 0.658±0.12 g/L under the same conditions. To increase the yield of prodigiosin production by M3349, significant medium components were determined using a two-level Plackett-Burman statistical design technique. Among fourteen components included in SMB medium, NaCl, Na2SiO3, MgCl2, H3BO3, Na2HPO4, Na2SO4, and CaCl2 were determined to be important for prodigiosin production. The medium formulation was finally optimized using a Box-Behnken design as follows: sucrose 10.0, peptone 8.0, yeast extract 2.0, NaCl 10.0, Na2SO4 12.0, CaCl2 1.8, MgCl2 0.7 g/L; and H3BO3 22.0, Na2HPO4 20.0, Na2SiO3 8.0 mg/L. The predicted maximum yield of prodigiosin in the optimized medium was 2.43 g/L by the Box-Behnken design, while the practical production was 2.60±0.176 g/L, which was 3.9 times higher than wild type with SMB Medium (0.658 g/L).

Keywords: Hahella chejuensis;prodigiosin;statistical experimental design

Figure & Data

References

Citations

Citations to this article as recorded by

Pigments from pathogenic bacteria: a comprehensive update on recent advances
Kusumita Acharya, Swarna Shaw, Sudipta Paul Bhattacharya, Shatarupa Biswas, Suman Bhandary, Arijit Bhattacharya
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef
Boosting algicidal efficiency of Alteromonas sp. FDHY-CJ against Skeletonema costatum through fermentation optimization
Pin Lv, Xinguo Shi, Qianqian Wang, Yuying Zhong, Yisong Guo, Jianfeng Chen
Protist.2024; 175(1): 126006. CrossRef
Pharmacological potential of ants and their symbionts – a review
Surbhi Agarwal, Garima Sharma, Kavita Verma, Narayanan Latha, Vartika Mathur
Entomologia Experimentalis et Applicata.2022; 170(12): 1032. CrossRef
High‐level production of microbial prodigiosin: A review
Rui Han, Roujin Xiang, Jinglin Li, Fengqing Wang, Chuan Wang
Journal of Basic Microbiology.2021; 61(6): 506. CrossRef
Improved Prodigiosin Production by Relieving CpxR Temperature-Sensitive Inhibition
Yang Sun, Lijun Wang, Xuewei Pan, Tolbert Osire, Haitian Fang, Huiling Zhang, Shang-Tian Yang, Taowei Yang, Zhiming Rao
Frontiers in Bioengineering and Biotechnology.2020;[Epub] CrossRef
The production and bioactivity of prodigiosin: quo vadis?
Shian-Ren Lin, Yu-Hsin Chen, Feng-Jen Tseng, Ching-Feng Weng
Drug Discovery Today.2020; 25(5): 828. CrossRef
Loss of Serine-Type D-Ala-D-Ala Carboxypeptidase DacA Enhances Prodigiosin Production in Serratia marcescens
Xuewei Pan, Changhao Sun, Mi Tang, Chao Liu, Jianing Zhang, Jiajia You, Tolbert Osire, Yang Sun, Youxi Zhao, Meijuan Xu, Taowei Yang, Zhiming Rao
Frontiers in Bioengineering and Biotechnology.2019;[Epub] CrossRef
Effect of mutations in extracellular nuclease on the characteristics of the pigmented and nonpigmented Serratia marcescens strains
E. Kh. Nizamutdinova, T. V. Shirshikova, A. M. Mardanova, M. R. Sharipova, L. M. Bogomol’naya
Microbiology.2016; 85(1): 42. CrossRef
Solid-state fermentation with Serratia marcescens Xd-1 enhanced production of prodigiosin by using bagasse as an inertia matrix
Yongjun Xia, Guangqiang Wang, Xiangna Lin, Xin Song, Lianzhong Ai
Annals of Microbiology.2016; 66(3): 1239. CrossRef
Towards an understanding of bacterial metabolites prodigiosin and violacein and their potential for use in commercial sunscreens
R. K. Suryawanshi, C. D. Patil, H. P. Borase, C. P. Narkhede, A. Stevenson, J. E. Hallsworth, S. V. Patil
International Journal of Cosmetic Science.2015; 37(1): 98. CrossRef
Optimizing Ultrasound-Assisted Extraction of Prodigiosin by Response Surface Methodology
Shi-Qing Sun, Yu-Jie Wang, Wei Xu, Chang-Jun Zhu, Xiao-Xia Liu
Preparative Biochemistry and Biotechnology.2015; 45(2): 101. CrossRef
Comparative Transcriptome of Wild Type and Selected Strains of the Microalgae Tisochrysis lutea Provides Insights into the Genetic Basis, Lipid Metabolism and the Life Cycle
Gregory Carrier, Matthieu Garnier, Loïc Le Cunff, Gaël Bougaran, Ian Probert, Colomban De Vargas, Erwan Corre, Jean-Paul Cadoret, Bruno Saint-Jean, María Mar Abad-Grau
PLoS ONE.2014; 9(1): e86889. CrossRef
Combination of uniform design with artificial neural network coupling genetic algorithm: an effective way to obtain high yield of biomass and algicidal compound of a novel HABs control actinomycete
Guanjing Cai, Wei Zheng, Xujun Yang, Bangzhou Zhang, Tianling Zheng
Microbial Cell Factories.2014;[Epub] CrossRef
Properties and applications of undecylprodigiosin and other bacterial prodigiosins
Nada Stankovic, Lidija Senerovic, Tatjana Ilic-Tomic, Branka Vasiljevic, Jasmina Nikodinovic-Runic
Applied Microbiology and Biotechnology.2014; 98(9): 3841. CrossRef
MUTANT BREEDING OFSerratia marcescensSTRAIN FOR ENHANCING PRODIGIOSIN PRODUCTION AND APPLICATION TO TEXTILES
Xiaoxia Liu, Yujie Wang, Shiqing Sun, Changjun Zhu, Wei Xu, Yongdoo Park, Haimeng Zhou
Preparative Biochemistry and Biotechnology.2013; 43(3): 271. CrossRef
Enhancing production of prodigiosin from Serratia marcescens C3 by statistical experimental design and porous carrier addition strategy
Wei-Chuan Chen, Wan-Ju Yu, Chia-Che Chang, Jo-Shu Chang, Shih-Horng Huang, Chih-Hung Chang, Shan-Yu Chen, Chih-Ching Chien, Chao-Ling Yao, Wen-Ming Chen, Yu-Hong Wei
Biochemical Engineering Journal.2013; 78: 93. CrossRef
Enhancement of the Stability and Solubility of Prodigiosin Using β-Cyclodextrin in Seawater
Hee-Yong Park, Tai-Kyoung Kim, Se-Jong Han, Joung-Han Yim
KSBB Journal.2012; 27(2): 109. CrossRef
Harmful dinoflagellate blooms caused by Cochlodinium sp.: Global expansion and ecological strategies facilitating bloom formation
Raphael M. Kudela, Christopher J. Gobler
Harmful Algae.2012; 14: 71. CrossRef
Development of natural anti-tumor drugs by microorganisms
Chia-Che Chang, Wei-Chuan Chen, Tsing-Fen Ho, Ho-Shing Wu, Yu-Hong Wei
Journal of Bioscience and Bioengineering.2011; 111(5): 501. CrossRef
Vibrio sp. DSM 14379 Pigment Production—A Competitive Advantage in the Environment?
Nejc Starič, Tjaša Danevčič, David Stopar
Microbial Ecology.2010; 60(3): 592. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Mutant Selection of Hahella chejuensis KCTC 2396 and Statistical Optimization of Medium Components for Prodigiosin Yield-Up

J. Microbiol. 2008;46(2):183-188. Published online June 11, 2008

DOI: https://doi.org/10.1007/s12275-008-0037-y

Related articles