Cyanobacterial Hybrid Kinase Sll0043 Regulates Phototaxis by Suppressing Pilin and Twitching Motility Protein

Bong-Jeong Shin; Jeehyun Oh; Sungsoo Kang; Young-Ho Chung; Young Mok Park; Young Hwan Kim; Seungil Kim; Jong Bhak; Jong-Soon Choi

doi:10.1007/s12275-007-0212-6

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Research Support, Non-U.S. Gov't
Cyanobacterial Hybrid Kinase Sll0043 Regulates Phototaxis by Suppressing Pilin and Twitching Motility Protein: Bong-Jeong Shin ¹, Jeehyun Oh ¹, Sungsoo Kang ², Young-Ho Chung ³, Young Mok Park ³, Young Hwan Kim ¹, Seungil Kim ¹, Jong Bhak ², Jong-Soon Choi ¹; Journal of Microbiology 2008;46(3):300-308.
DOI: https://doi.org/10.1007/s12275-007-0212-6
Published online: July 5, 2008

¹Proteomics Team, Korea Basic Science Institute, Daejeon 305-333, Republic of Korea, ²Korean BioInformation Center, KRIBB, Daejeon 305-806, Republic of Korea, ³Systems Biology Team, Korea Basic Science Institute, Daejeon 305-333, Republic of Korea

Corresponding author: Jong-Soon Choi , Tel: 82-42-865-3428,

Received: 6 November 2007 • Accepted: 14 March 2008

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Abstract

The unicellular cyanobacterium Synechocystis sp. PCC 6803 glides toward a light source through the interplay of positive phototaxis genes and proteins. In genetic analysis, the complete disruption of the hybrid sensory kinase sll0043 produced negative phototaxis. Furthermore, Sll0043 was found to be a hub protein by in silico prediction of protein-protein interaction, in which Sll0043 was predominantly linked to seven two-component proteins with high confidence. To understand the regulation and networking of positive phototaxis proteins, the proteomic profile of the sll0043 mutant was compared to that of wild-type. In the sll0043 mutant, 18 spots corresponding to 15 unique proteins were altered by 1.3 to 59 fold; the spots were identified by 2-DE/MALDI-MS analysis. Down-regulated proteins in the sll0043 null-mutant included chaperonins, superoxide dismutase, and phycocyanin β-subunit. In contrast, nine proteins involved in photosynthesis, translation, regulatory function, and other functions were up-regulated. In particular, a twitching motility protein (PilT1) was induced over 2-fold in sll0043 mutant. Moreover, semiquantitative and quantitative RT-PCR analysis revealed that pilin (pilA1), pili motor (pilT1), and pili switch gene (pilT2) were significantly increased in sll0043 mutant. These results suggest that the hybrid kinase Sll0043 regulates positive phototaxis by suppressing the expression of pili biosynthesis and regulatory genes and through the interplay with positive phototaxis/motility two-component proteins.

Keywords: cyanobacteria;two-component system;positive phototaxis;2-DE/MALDI-MS;RT-PCR;proteinprotein interaction network

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Cyanobacterial Hybrid Kinase Sll0043 Regulates Phototaxis by Suppressing Pilin and Twitching Motility Protein

J. Microbiol. 2008;46(3):300-308. Published online July 5, 2008

DOI: https://doi.org/10.1007/s12275-007-0212-6

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