Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search
Advanced Search
mobile menu button

Articles

Page Path
HOME > J. Microbiol > Volume 46(4); 2008 > Article
Research Support, Non-U.S. Gov't
Cloning and Characterization of a Na+/H+ Antiporter Gene of the Moderately Halophilic Bacterium Halobacillus aidingensis AD-6T
Ya Jie Zou 1, Li Fu Yang 1,2, Lei Wang 1, Su Sheng Yang 1
Journal of Microbiology 2008;46(4):415-421.
DOI: https://doi.org/10.1007/s12275-008-0009-2
Published online: August 31, 2008
1Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, and Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, Beijing 100094, P. R. China, 2Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, and Key Laboratory of Physiology for Tropical Crops, Ministry of Agriculture, Danzhou Hainan 571737, P. R. China
Corresponding author:  Su Sheng Yang , Tel: 86-10-6273-2173, 
Received: 3 January 2008   • Accepted: 24 June 2008
prev next
  • 85 Views
  • 0 Download
  • 13 Crossref
  • 15 Scopus
Full Article

Abstract

A gene encoding a Na+/H+ antiporter was obtained from the genome of Halobacillus aidingensis AD-6T, which was sequenced and designated as nhaH. The deduced amino acid sequence of the gene was 91% identical to the NhaH of H. dabanensis, and shared 54% identity with the NhaG of Bacillus subtilis. The cloned gene enable the Escherichia coli KNabc cell, which lack all of the major Na+/H+ antiporters, to grow in medium containing 0.2 M NaCl or 10 mM LiCl. The nhaH gene was predicted to encode a 43.5 kDa protein (403 amino acid residues) with 11 putative transmembrane regions. Everted membrane vesicles prepared from E. coli KNabc cells carrying NhaH exhibited Na+/H+ as well as Li+/H+ antiporter activity, which was pH-dependent with the highest activity at pH 8.0, and no K+ /H+ antiporter activity was detected. The deletion of hydrophilic C-terminal amino acid residues showed that the short C-terminal tail was vital for Na+/H+ antiporter activity.

  • Cite this Article
    Cite this Article
    export Copy Download
    Close
    Download Citation
    Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
    Format:
    • RIS — For EndNote, ProCite, RefWorks, and most other reference management software
    • BibTeX — For JabRef, BibDesk, and other BibTeX-specific software
    Include:
    • Citation for the content below
    Cloning and Characterization of a Na+/H+ Antiporter Gene of the Moderately Halophilic Bacterium Halobacillus aidingensis AD-6T
    J. Microbiol. 2008;46(4):415-421.   Published online August 31, 2008
    DOI: https://doi.org/10.1007/s12275-008-0009-2
    Close
Related articles
Journal of Microbiology : Journal of Microbiology
© The Microbiological Society of Korea.
TOP