Induction of IL-8 in Periodontal Ligament Cells by H2O2

Yang-Sin Lee; Eun Jung Bak; Minyoung Kim; Wonse Park; Jeong Taeg Seo; Yun-Jung Yoo

doi:10.1007/s12275-008-0182-3

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Research Support, Non-U.S. Gov't
Induction of IL-8 in Periodontal Ligament Cells by H2O2: Yang-Sin Lee ¹, Eun Jung Bak ¹, Minyoung Kim ^1,2, Wonse Park ³, Jeong Taeg Seo ^1,2, Yun-Jung Yoo ^1,2; Journal of Microbiology 2008;46(5):579-584.
DOI: https://doi.org/10.1007/s12275-008-0182-3
Published online: October 31, 2008

¹Department of Oral Biology, BK21 project, Oral Science Research Center, Yonsei University College of Dentistry, Seoul 120-752, Republic of Korea, ²Department of Applied Life Science, The Graduate School, Yonsei University, Seoul 120-752, Republic of Korea, ³Department of General Dentistry, Dental Hospital, Yonsei University, Seoul 120-752, Republic of Korea

Corresponding author: Yun-Jung Yoo , Tel: 82-2-2228-3060,

Received: 18 July 2008 • Accepted: 9 September 2008

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Abstract

Periodontitis is an inflammatory disease caused by bacteria. In periodontitis, reactive oxygen species (ROS) are released from inflammatory cells in response to bacteria. Interleukin (IL)-8 is one of pro-inflammatory cytokines. To investigate the role of ROS in pathogenesis of periodontitis, we estimated the effect of H2O2, one of ROS, on the expression of IL-8 in human periodontal ligament (PDL) cells. PDL cells were treated with H2O2. IL-8 expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38) and c-jun NH2-terminal kinase (JNK) was estimated by Western blotting. Treatment with H2O2 at concentration of up to 250 μM increased IL-8 mRNA expression and production in a concentration-dependent manner. However, treatment with 500 μM H2O2 did not increase IL-8 production. Catalase, an inhibitor of H2O2, down-regulated the production of IL-8 induced by H2O2. H2O2 increased the phosphorylation of ERK, p38, and JNK. Pretreatment with PD98059 (ERK inhibitor), SB203580 (p38 inhibitor), or SP600125 (JNK inhibitor) decreased the IL-8 production induced by H2O2. These results indicate that H2O2 acts as an inducer of IL-8 secretion via activation of ERK, p38, and JNK in PDL cells. H2O2 deposited in periodontal tissue during inflammation against bacteria may accelerate tissue destruction via induction of IL-8 in PDL cells.

Keywords: IL-8;periodontal ligament cells;H2O2

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Induction of IL-8 in Periodontal Ligament Cells by H2O2

J. Microbiol. 2008;46(5):579-584. Published online October 31, 2008

DOI: https://doi.org/10.1007/s12275-008-0182-3

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