Generation of Infectious Transcripts from Korean Strain and Mild Mottle Strain of Potato Virus X

Sun Hee Choi; Ki Hyun Ryu

doi:10.1007/s12275-008-0078-2

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Generation of Infectious Transcripts from Korean Strain and Mild Mottle Strain of Potato Virus X: Sun Hee Choi , Ki Hyun Ryu; Journal of Microbiology 2008;46(5):502-507.
DOI: https://doi.org/10.1007/s12275-008-0078-2
Published online: October 31, 2008

Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women’s University, Seoul 139-774, Republic of Korea

Corresponding author: Ki Hyun Ryu , Tel: 82-2-970-5618,

Received: 27 March 2008 • Accepted: 3 July 2008

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Abstract

Full-length cDNAs of two different strains of Potato virus X (PVX-Kr and PVX-Mo) have been directly amplified by long template reverse transcription polymerase chain reaction (RT-PCR) using the 5’-end primer containing a SP6 or T7 RNA promoter sequence and the virus-specific 3’-end primer, and then constructed in plasmid vectors. Capped in vitro transcripts from cloned full-length cDNAs as well as those RTPCR amplicons proved to be infectious systemically on tobacco plants. Symptom expression on tobacco plants from PVX-Mo transcripts was faster and severer than that from PVX-Kr. In replication stability test of transcripts derived from PVX clones, progeny viruses showed stable replication according to sequencing through passages. This highly infectious transcript system from the full-length cDNA clones for PVX can be useful for recombinant molecules for functional analysis of viral proteins in plant-virus interaction study as well as for expression of foreign protein in planta.

Keywords: Potato virus X;in vitro transcription;infectious cDNA clone;plant virus;mild mottle strain;Korean strain

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Generation of Infectious Transcripts from Korean Strain and Mild Mottle Strain of Potato Virus X

J. Microbiol. 2008;46(5):502-507. Published online October 31, 2008

DOI: https://doi.org/10.1007/s12275-008-0078-2

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