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Molecular Characterization of ORFs 2 to 7 of Korean Porcine Reproductive and Respiratory Syndrome Virus (CA) and Its Protein Expression by Recombinant Baculoviruses
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HOME > J. Microbiol > Volume 46(6); 2008 > Article
Research Support, Non-U.S. Gov't
Molecular Characterization of ORFs 2 to 7 of Korean Porcine Reproductive and Respiratory Syndrome Virus (CA) and Its Protein Expression by Recombinant Baculoviruses
Hyun Na Koo 1, Jeong Mi Oh 1, Jae Kyung Lee 1, Jae Young Choi 2,3, Kwang Sik Lee 4, Jong Yul Roh 2, Yeon Ho Je 2, Byung Rae Jin 4, Sung Sik Yoo 5, Jae Su Kim 6, Young In Kim 6, In Joong Yoon 5, Soo Dong Woo 1
Journal of Microbiology 2008;46(6):709-719
DOI: https://doi.org/10.1007/s12275-008-0224-x
Published online: December 24, 2008
1Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea, 2School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea, 3Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea, 4College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea, 5Choong-Ang Vaccine Laboratory, Daejeon 305-348, Republic of Korea, 6AgroLife Research Institute (ARI), Dongbu HiTek Co. Ltd., Daejeon 305-708, Republic of Korea1Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea, 2School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea, 3Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea, 4College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea, 5Choong-Ang Vaccine Laboratory, Daejeon 305-348, Republic of Korea, 6AgroLife Research Institute (ARI), Dongbu HiTek Co. Ltd., Daejeon 305-708, Republic of Korea
Corresponding author:  Soo Dong Woo , Tel: 82-43-261-2553, 
Received: 11 September 2008   • Accepted: 30 September 2008
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Abstract

To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR-2332. Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support. Additionally, expression levels of the PRRSV proteins in insect cells were strong or partially weak. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.

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    Molecular Characterization of ORFs 2 to 7 of Korean Porcine Reproductive and Respiratory Syndrome Virus (CA) and Its Protein Expression by Recombinant Baculoviruses
    J. Microbiol. 2008;46(6):709-719.   Published online December 24, 2008
    DOI: https://doi.org/10.1007/s12275-008-0224-x
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