Molecular Characterization of ORFs 2 to 7 of Korean Porcine Reproductive and Respiratory Syndrome Virus (CA) and Its Protein Expression by Recombinant Baculoviruses

Hyun Na Koo; Jeong Mi Oh; Jae Kyung Lee; Jae Young Choi; Kwang Sik Lee; Jong Yul Roh; Yeon Ho Je; Byung Rae Jin; Sung Sik Yoo; Jae Su Kim; Young In Kim; In Joong Yoon; Soo Dong Woo

doi:10.1007/s12275-008-0224-x

Articles

Page Path: HOME > J. Microbiol > Volume 46(6); 2008 > Article

Research Support, Non-U.S. Gov't
Molecular Characterization of ORFs 2 to 7 of Korean Porcine Reproductive and Respiratory Syndrome Virus (CA) and Its Protein Expression by Recombinant Baculoviruses: Hyun Na Koo ¹, Jeong Mi Oh ¹, Jae Kyung Lee ¹, Jae Young Choi ^2,3, Kwang Sik Lee ⁴, Jong Yul Roh ², Yeon Ho Je ², Byung Rae Jin ⁴, Sung Sik Yoo ⁵, Jae Su Kim ⁶, Young In Kim ⁶, In Joong Yoon ⁵, Soo Dong Woo ¹; Journal of Microbiology 2008;46(6):709-719.
DOI: https://doi.org/10.1007/s12275-008-0224-x
Published online: December 24, 2008

¹Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea, ²School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea, ³Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea, ⁴College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea, ⁵Choong-Ang Vaccine Laboratory, Daejeon 305-348, Republic of Korea, ⁶AgroLife Research Institute (ARI), Dongbu HiTek Co. Ltd., Daejeon 305-708, Republic of Korea¹Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea, ²School of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea, ³Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea, ⁴College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea, ⁵Choong-Ang Vaccine Laboratory, Daejeon 305-348, Republic of Korea, ⁶AgroLife Research Institute (ARI), Dongbu HiTek Co. Ltd., Daejeon 305-708, Republic of Korea

Corresponding author: Soo Dong Woo , Tel: 82-43-261-2553,

Received: 11 September 2008 • Accepted: 30 September 2008

prev next

46 Views
0 Download
5 Crossref
4 Scopus

Full Article

Abstract

To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR-2332. Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 89.8 to 99.5%. To better understand the genetic relationships between other strains, phylogenetic analyses were performed. The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support. Additionally, expression levels of the PRRSV proteins in insect cells were strong or partially weak. The results of this study have implications for both the taxonomy of PRRSV and vaccine development.

Keywords: PRRSV;CA strain;phylogenetic analysis;baculovirus;bApGOZA system

Figure & Data

References

Citations

Citations to this article as recorded by

Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016
Hyeonjeong Kang, Ji Eun Yu, Ji-Eun Shin, Areum Kang, Won-Il Kim, Changhee Lee, Jienny Lee, In-Soo Cho, Se-Eun Choe, Sang-Ho Cha
BMC Veterinary Research.2018;[Epub] CrossRef
Enhanced expression of the structural protein of porcine reproductive and respiratory syndrome virus (PRRSV) by SUMO fusion
Hyun Na Koo, Sung Min Bae, Soo Dong Woo
International Journal of Industrial Entomology.2016; 32(2): 90. CrossRef
The 15N and 46R Residues of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Enhance Regulatory T Lymphocytes Proliferation
Baochao Fan, Xing Liu, Juan Bai, Yufeng Li, Qiaoya Zhang, Ping Jiang, Guihong Zhang
PLOS ONE.2015; 10(9): e0138772. CrossRef
Genomic analysis and pathogenic characteristics of Type 2 porcine reproductive and respiratory syndrome virus nsp2 deletion strains isolated in Korea
Hwan-Won Choi, Eeuri Nam, Yoo Jin Lee, Yun-Hee Noh, Seung-Chul Lee, In-Joong Yoon, Hyun-Soo Kim, Shien-Young Kang, Young-Ki Choi, Changhee Lee
Veterinary Microbiology.2014; 170(3-4): 232. CrossRef
Sequence Analysis and Expression of ORF5 in Korean Isolate of Porcine Reproductive and Respiratory Syndrome Virus
Sun Shin Yi, Ji-Sun Sun, Hyun-Soo Kim, Shien-Young Kang, Tae-Wook Hahn
Journal of Bacteriology and Virology.2011; 41(3): 173. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Molecular Characterization of ORFs 2 to 7 of Korean Porcine Reproductive and Respiratory Syndrome Virus (CA) and Its Protein Expression by Recombinant Baculoviruses

J. Microbiol. 2008;46(6):709-719. Published online December 24, 2008

DOI: https://doi.org/10.1007/s12275-008-0224-x

Related articles