Prevalence of Tetracycline Resistance Genes in Greek Seawater Habitats

Theodora L. Nikolakopoulou; Eleni P. Giannoutsou; Adamandia A. Karabatsou; Amalia D. Karagouni

doi:10.1007/s12275-008-0080-8

Articles

Page Path: HOME > J. Microbiol > Volume 46(6); 2008 > Article

Research Support, Non-U.S. Gov't
Prevalence of Tetracycline Resistance Genes in Greek Seawater Habitats: Theodora L. Nikolakopoulou , Eleni P. Giannoutsou , Adamandia A. Karabatsou , Amalia D. Karagouni; Journal of Microbiology 2008;46(6):633-640.
DOI: https://doi.org/10.1007/s12275-008-0080-8
Published online: December 24, 2008

National and Kapodistrian University of Athens, Faculty of Biology, Department of Botany, Microbiology Group, Athens 15781, Greece

Corresponding author: Amalia D. Karagouni , Tel: 30-210-727-4526,

Received: 2 April 2008 • Accepted: 25 September 2008

prev next

96 Views
0 Download
15 Crossref
21 Scopus

Full Article

Abstract

The presence of selected tetracycline resistance (TcR) genes was studied in different Greek seawater habitats, originated from wastewater treatment facilities, fishfarm, and coastal environments. The methods employed included assessment of the presence of twelve gene clusters by PCR, followed by hybridization with specific probes, in habitat extracted DNA, TcR bacteria, and exogenous isolated plasmids conferring TcR. The direct DNA-based analysis showed that tet(Α) and tet(K) genes were detected in all habitats, whilst tet(C) and tet(E) were present in fishfarm and wastewater effluent samples and tet(M) was detected in fishfarm and coastal samples. Resistance genes tet(A), tet(C), tet(K), and tet(M) were detected in 60 of the 89 isolates screened. These isolates were identified by fatty acid methyl ester analysis (FAME) as Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus, and Staphylococcus strains. The presence of the TcR genes in 15% of the bacterial isolates coincided with the presence of IncP plasmids. A habitat-specific dissemination of IncP alpha plasmids in wastewater effluent isolates and of IncP beta plasmids in fishfarm isolates was observed. Exogenous isolation demonstrated the presence of plasmids harbouring TcR genes in all the habitats tested. Plasmids were shown to carry tet(A), tet(C), tet(E), and tet(K) genes. It is concluded that TcR genes are widespread in the seawater habitats studied and often occur on broad host range plasmids that seem to be well disseminated in the bacterial communities.

Keywords: tetracycline resistance genes;seawater samples;exogenous plasmid isolation;polymerase chain reaction analysis;total community DNA

Figure & Data

References

Citations

Citations to this article as recorded by

Antimicrobial Resistance in Romania: Updates on Gram-Negative ESCAPE Pathogens in the Clinical, Veterinary, and Aquatic Sectors
Ilda Czobor Barbu, Irina Gheorghe-Barbu, Georgiana Alexandra Grigore, Corneliu Ovidiu Vrancianu, Mariana Carmen Chifiriuc
International Journal of Molecular Sciences.2023; 24(9): 7892. CrossRef
Isolation and characterization of a novel Tenacibaculum species and a corresponding bacteriophage from a Mediterranean fish hatchery: Description of Tenacibaculum larymnensis sp. nov. and Tenacibaculum phage Larrie
Maria Ioanna Tsertou, Adriana Triga, Stavros Droubogiannis, Constantina Kokkari, Grammatiki Anasi, Pantelis Katharios
Frontiers in Microbiology.2023;[Epub] CrossRef
Composition and distribution of bacterial communities and antibiotic resistance genes in fish of four mariculture systems
Mingqing Zhang, Liping Hou, Yating Zhu, Cuiping Zhang, Wen Li, Xingxing Lai, Jinlin Yang, Shuisheng Li, Hu Shu
Environmental Pollution.2022; 311: 119934. CrossRef
Antibiotic-Resistant Bacteria in Aquaculture and Climate Change: A Challenge for Health in the Mediterranean Area
Milva Pepi, Silvano Focardi
International Journal of Environmental Research and Public Health.2021; 18(11): 5723. CrossRef
Acinetobacter baumannii Antibiotic Resistance Mechanisms
Ioannis Kyriakidis, Eleni Vasileiou, Zoi Dorothea Pana, Athanasios Tragiannidis
Pathogens.2021; 10(3): 373. CrossRef
Antibiotic-Resistant Bacteria in Greywater and Greywater-Irrigated Soils
Eleonora Troiano, Luciano Beneduce, Amit Gross, Zeev Ronen
Frontiers in Microbiology.2018;[Epub] CrossRef
Interplay of a non-conjugative integrative element and a conjugative plasmid in the spread of antibiotic resistance via suicidal plasmid transfer from an aquaculture Vibrio isolate
Lisa Nonaka, Tatsuya Yamamoto, Fumito Maruyama, Yuu Hirose, Yuki Onishi, Takeshi Kobayashi, Satoru Suzuki, Nobuhiko Nomura, Michiaki Masuda, Hirokazu Yano, Axel Cloeckaert
PLOS ONE.2018; 13(6): e0198613. CrossRef
Multiple Antimicrobial Resistance and Novel Point Mutation in Fluoroquinolone-ResistantEscherichia coliIsolates from Mangalore, India
Kogaluru Shivakumaraswamy Santhosh, Vijaya Kumar Deekshit, Moleyuru Nagarajappa Venugopal, Iddya Karunasagar, Indrani Karunasagar
Microbial Drug Resistance.2017; 23(8): 994. CrossRef
Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment
Monika Harnisz, Ewa Korzeniewska, Sebastian Niestępski, Adriana Osińska, Beata Nalepa, B. Kaźmierczak, M. Kutyłowska, K. Piekarska, H. Jouhara, J. Danielewicz
E3S Web of Conferences.2017; 22: 00063. CrossRef
Exogenous isolation of conjugative plasmids from pesticide contaminated soil
Reshma Anjum, Elisabeth Grohmann, Abdul Malik
World Journal of Microbiology and Biotechnology.2012; 28(2): 567. CrossRef
Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2012 update)

EFSA Journal.2012;[Epub] CrossRef
Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2011 update)

EFSA Journal.2011; 9(12): 2497. CrossRef
Tetracycline Resistance-Encoding Plasmid from Bacillus sp. Strain #24, Isolated from the Marine Sponge Haliclona simulans
Robert W. Phelan, Charles Clarke, John P. Morrissey, Alan D. W. Dobson, Fergal O'Gara, Teresa M. Barbosa
Applied and Environmental Microbiology.2011; 77(1): 327. CrossRef
Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2010 update)

EFSA Journal.2010;[Epub] CrossRef
The pattern, association with multidrug-resistance and transferability of plasmid-mediated tetracycline resistance inEscherichia coli isolates from the poultry in Iran
Tahereh Falsafi, Monir Ebrahimi, Ezat Asgarani, Vahid Mirtorabi
Annals of Microbiology.2009;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Prevalence of Tetracycline Resistance Genes in Greek Seawater Habitats

J. Microbiol. 2008;46(6):633-640. Published online December 24, 2008

DOI: https://doi.org/10.1007/s12275-008-0080-8

Related articles