Molecular Identification of Fecal Pollution Sources in Water Supplies by Host-Specific Fecal DNA Markers and Terminal Restriction Fragment Length Polymorphism Profiles of 16S rRNA Gene

Ju-Yong Jeong; Kyung-Ik Gil; Kyong-Hee Lee; Jong-Ok Ka

doi:10.1007/s12275-008-0174-3

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Molecular Identification of Fecal Pollution Sources in Water Supplies by Host-Specific Fecal DNA Markers and Terminal Restriction Fragment Length Polymorphism Profiles of 16S rRNA Gene: Ju-Yong Jeong ^1,2, Kyung-Ik Gil ³, Kyong-Hee Lee ², Jong-Ok Ka ¹; Journal of Microbiology 2008;46(6):599-607.
DOI: https://doi.org/10.1007/s12275-008-0174-3
Published online: December 24, 2008

¹Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea, ²Department of Water Quality Research, Gyeonggi-do Institute of Health and Environment, Suwon 440-290, Republic of Korea, ³Department of Civil Engineering, Seoul National University of Technology, Seoul 139-743, Republic of Korea

Corresponding author: Jong-Ok Ka , Tel: 82-2-880-4673,

Received: 11 July 2008 • Accepted: 25 August 2008

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Abstract

Specific fecal DNA markers were investigated for major pollution sources, cow, human, and pig, and occurrence of the identified markers was analyzed in river waters using Terminal Restriction Fragment Length Polymorphism (T-RFLP) techniques and sequencing of 16S rDNA of Bacteroides-Prevotella. The unique and specific DNA markers for cow and human were identified as a 222 bp and 60 bp peak in HaeIII T-RFLP profiles, respectively, and the pig-specific marker was not identified but the unique T-RFLP profile of pig could be used as a substitution. Human-specific marker was detected in most of the river waters tested (92.1%) and T-RFLP profiles of river waters were shown to be similar to those of human feces. Cluster analysis of T-RFLP data showed that the fecal sources were multiple (human plus cow and human plus dairy cow) in most of the river waters. The phylogenetic analysis for the clones recovered from the fecal and water samples showed that the clones from cow formed a discreet cluster from those of other sources. The other clones from human, pig, and river water formed two groups all together. The results of this study could be used to identify and control the fecal pollution source in the bodies of water in Korea.

Keywords: 16S rDNA;Bacteroides-Prevotella;fecal DNA marker;T-RFLP

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Microbial community analysis and identification of alternative host-specific fecal indicators in fecal and river water samples using pyrosequencing
Ju-Yong Jeong, Hee-Deung Park, Kyong-Hee Lee, Hang-Yeon Weon, Jong-Ok Ka
The Journal of Microbiology.2011; 49(4): 585. CrossRef
Application of an Integrated Community Analysis Approach for Microbial Source Tracking in a Coastal Creek
Yiping Cao, Laurie C. Van De Werfhorst, Bram Sercu, Jill L. S. Murray, Patricia A. Holden
Environmental Science & Technology.2011; 45(17): 7195. CrossRef

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Molecular Identification of Fecal Pollution Sources in Water Supplies by Host-Specific Fecal DNA Markers and Terminal Restriction Fragment Length Polymorphism Profiles of 16S rRNA Gene

J. Microbiol. 2008;46(6):599-607. Published online December 24, 2008

DOI: https://doi.org/10.1007/s12275-008-0174-3

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