Molecular Characterization of Vibrio cholerae Isolates from Cholera Outbreaks in North India

Joseph J. Kingston; Kuruvilla Zachariah; Urmil Tuteja; Sanjay Kumar; Harsh Vardhan Batra

doi:10.1007/s12275-008-0162-7

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Molecular Characterization of Vibrio cholerae Isolates from Cholera Outbreaks in North India: Joseph J. Kingston ¹, Kuruvilla Zachariah ¹, Urmil Tuteja ², Sanjay Kumar ², Harsh Vardhan Batra ¹; Journal of Microbiology 2009;47(1):110-115.
DOI: https://doi.org/10.1007/s12275-008-0162-7
Published online: February 20, 2009

¹Division of Microbiology, Defence Food Research Laboratory, Sidhartha Nagar, Mysore, Karnataka 570011, India, ²Division of Microbiology, Defence R&D Establishment, Jhansi Road, Gwalior, Madhya Pradesh 474002, India¹Division of Microbiology, Defence Food Research Laboratory, Sidhartha Nagar, Mysore, Karnataka 570011, India, ²Division of Microbiology, Defence R&D Establishment, Jhansi Road, Gwalior, Madhya Pradesh 474002, India

Corresponding author: Harsh Vardhan Batra , Tel: 91-821-247-9779,

Received: 1 July 2008 • Accepted: 10 November 2008

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Abstract

Vibrio cholerae isolates recovered from cholera outbreaks in Bhind district of Madhya Pradesh and Delhi, Northern India were characterized. The O1 serogroup isolates from Bhind outbreak were of Inaba serotype whereas both Ogawa and Inaba serotypes were recovered from Delhi. PCR analysis revealed that only O1 serogroup V. cholerae isolates carried the virulence-associated genes like ctxA, tcpA, ace, and zot. Molecular typing by repetitive sequence based ERIC, VCR1, and VC1 PCR’s revealed similar DNA profile for both Inaba and Ogawa serotypes. A discrete VC1-PCR band identified among the El Tor strains had greater similarity (>97%) to the V. cholerae genome sequence and therefore has the potential to be used as a marker for the identification of the V. cholerae strains. Non-O1 strains recovered from Bhind region differed among themselves as well as from that of the O1 isolates. All the O1 serogroup isolates possessed SXT element and were uniformly resistant to the antibiotics nalidixic acid, polymyxin-B, furazolidone, cloxacilin, trimethoprim-sulfamethaxazole, and vibriostatic agent O129. Inaba strains from both Delhi and Bhind differed from Ogawa strains by their resistance to streptomycin despite sharing similar DNA patterns in all the three rep-PCRs. Though Delhi and Bhind are separate geographical regions in Northern India, Inaba strains from both these places appear to be closely related owing to their similarity in antibiogram and genetic profile.

Keywords: ERIC-PCR;DNA fingerprinting;SXT;VC1-PCR;Vibrio cholerae

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Molecular Characterization of Vibrio cholerae Isolates from Cholera Outbreaks in North India

J. Microbiol. 2009;47(1):110-115. Published online February 20, 2009

DOI: https://doi.org/10.1007/s12275-008-0162-7

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