Induction of Growth Phase-Specific Autolysis in Bacillus subtilis 168 by Growth Inhibitors

Jin-Kyo Chung; Hyun Ee Yoon; Ha Chul Shin; Eun-Young Choi; Woo-Hyeon Byeon

doi:10.1007/s12275-008-0256-2

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Research Support, Non-U.S. Gov't
Induction of Growth Phase-Specific Autolysis in Bacillus subtilis 168 by Growth Inhibitors: Jin-Kyo Chung ¹, Hyun Ee Yoon ², Ha Chul Shin ³, Eun-Young Choi ⁴, Woo-Hyeon Byeon ⁵; Journal of Microbiology 2009;47(1):50-59
DOI: https://doi.org/10.1007/s12275-008-0256-2
Published online: February 20, 2009

¹Department of Physiology, College of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea, ²Central Lab., Seoul National University Hospital, Seoul 110-744, Republic of Korea, ³FCB-Pharmicell Co., Ltd., Seongnam-si, Gyeonggi-do 462-120, Republic of Korea, ⁴Department of Molecular Microbiology and Immunology, Life Sciences Center, School of Medicine, University of Missouri-Columbia, Columbia, Missouri 65212, USA, ⁵Department of Molecular Biosciences, Kangwon National University, Chuncheon 200-701, Republic of Korea¹Department of Physiology, College of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea, ²Central Lab., Seoul National University Hospital, Seoul 110-744, Republic of Korea, ³FCB-Pharmicell Co., Ltd., Seongnam-si, Gyeonggi-do 462-120, Republic of Korea, ⁴Department of Molecular Microbiology and Immunology, Life Sciences Center, School of Medicine, University of Missouri-Columbia, Columbia, Missouri 65212, USA, ⁵Department of Molecular Biosciences, Kangwon National University, Chuncheon 200-701, Republic of Korea

Corresponding author: Woo-Hyeon Byeon , Tel: 82-33-250-8542,

Received: 22 October 2008 • Accepted: 11 January 2009

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Abstract

Growth phase-specific autolysis of Bacillus subtilis by inhibitors of membrane permeability, inhibitors of macromolecule biosynthesis, inhibitors of cell wall biosynthesis and detergents were tested and characterized in glucose limited liquid medium. The minimum autolysis induction concentration (MAIC) of test compounds, which was at least 1/20th lower than the conventional autolysis induction concentration, induced autolysis only for cells at the glucose exhaustion point (diauxic point) of the growth phase, while it was not induced for cells at pre- and post-diauxic points. Inhibitors of macromolecule synthesis that are not known for inducing autolysis, such as chloramphenicol, rifampicin, nalidixic acid, and detergents, also induced specific autolysis. Two types of autolysis corresponding to the concentrations of compounds are distinguished: concentration-sensitive and concentration-insensitive types.

Keywords: autolysis; B. subtilis 168; diauxic point

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Induction of Growth Phase-Specific Autolysis in Bacillus subtilis 168 by Growth Inhibitors

J. Microbiol. 2009;47(1):50-59. Published online February 20, 2009

DOI: https://doi.org/10.1007/s12275-008-0256-2

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