Isolation and Identification of an Anticancer Drug, Taxol from Phyllosticta tabernaemontanae, a Leaf Spot Fungus of an Angiosperm, Wrightia tinctoria

Rangarajulu Senthil Kumaran; Johnpaul Muthumary; Byung-Ki Hur

doi:10.1007/s12275-008-0127-x

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Isolation and Identification of an Anticancer Drug, Taxol from Phyllosticta tabernaemontanae, a Leaf Spot Fungus of an Angiosperm, Wrightia tinctoria: Rangarajulu Senthil Kumaran ¹, Johnpaul Muthumary ², Byung-Ki Hur ¹; Journal of Microbiology 2009;47(1):40-49
DOI: https://doi.org/10.1007/s12275-008-0127-x
Published online: February 20, 2009

¹Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea, ²Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600-025, India¹Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea, ²Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600-025, India

Corresponding author: Rangarajulu Senthil Kumaran , Tel: 82-42-350-3973,

Received: 21 May 2008 • Accepted: 23 October 2008

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Abstract

Phyllosticta tabernaemontanae, a leaf spot fungus isolated from the diseased leaves of Wrightia tinctoria, showed the production of taxol, an anticancer drug, on modified liquid medium (M1D) and potato dextrose broth (PDB) medium in culture for the first time. The presence of taxol was confirmed by spectroscopic and chromatographic methods of analysis. The amount of taxol produced by this fungus was quantified using high performance liquid chromatography (HPLC). The maximum amount of taxol production was recorded in the fungus grown on M1D medium (461 ug/L) followed by PDB medium (150 ug/L). The production rate was increased to 9.2x103 fold than that found in the culture broth of earlier reported fungus, Taxomyces andreanae. The results designate that P. tabernaemontanae is an excellent candidate for taxol production. The fungal taxol extracted also showed a strong cytotoxic activity in the in vitro culture of tested human cancer cells by apoptotic assay.

Keywords: anticancer drug; leaf spot fungus; in vitro cytotoxicity test; Phyllosticta tabernaemontanae; taxol production

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Isolation and Identification of an Anticancer Drug, Taxol from Phyllosticta tabernaemontanae, a Leaf Spot Fungus of an Angiosperm, Wrightia tinctoria

J. Microbiol. 2009;47(1):40-49. Published online February 20, 2009

DOI: https://doi.org/10.1007/s12275-008-0127-x

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