Response of Saccharomyces cerevisiae to Stress-Free Acidification

Allen Kuan-Liang Chen; Cristy Gelling; Peter L. Rogers; Ian W. Dawes; Bettina Rosche

doi:10.1007/s12275-008-0167-2

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Research Support, Non-U.S. Gov't
Response of Saccharomyces cerevisiae to Stress-Free Acidification: Allen Kuan-Liang Chen ¹, Cristy Gelling ¹, Peter L. Rogers ¹, Ian W. Dawes ^1,2, Bettina Rosche ¹; Journal of Microbiology 2009;47(1):1-8.
DOI: https://doi.org/10.1007/s12275-008-0167-2
Published online: February 20, 2009

¹School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia, ²Ramaciotti Centre for Gene Function Analysis, University of New South Wales, Sydney NSW 2052, Australia¹School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia, ²Ramaciotti Centre for Gene Function Analysis, University of New South Wales, Sydney NSW 2052, Australia

Corresponding author: Bettina Rosche , Tel: 61-2-9385-1598,

Received: 3 July 2008 • Accepted: 27 September 2008

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Abstract

Genome-wide transcriptional analysis of a Saccharomyces cerevisiae batch culture revealed that more than 829 genes were regulated in response to an environmental shift from pH 6 to pH 3 by added sulfuric acid. This shift in pH was not detrimental to the rate of growth compared to a control culture that was maintained at pH 6 and the transcriptional changes most strikingly implicated not up- but down-regulation of stress responses. In addition, the transcriptional changes upon acid addition indicated remodeling of the cell wall and central carbon metabolism. The overall trend of changes was similar for the pH-shift experiment and the pH 6 control. However, the changes in the pH 6 control were much weaker and occurred 2.5 h later than in the pH-shift experiment. Thus, the reaction to the steep pH decrease was an immediate response within the normal repertoire of adaptation shown in later stages of fermentation at pH 6. Artificially preventing the yeast from acidifying the medium may be considered physiologically stressful under the tested conditions.

Keywords: stress response;Saccharomyces cerevisiae;acidification;fermentation;pyruvate decarboxylase

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Response of Saccharomyces cerevisiae to Stress-Free Acidification

J. Microbiol. 2009;47(1):1-8. Published online February 20, 2009

DOI: https://doi.org/10.1007/s12275-008-0167-2

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