Comparative Analysis of 2,4,6-Trinitrotoluene (TNT)-Induced Cellular Responses and Proteomes in Pseudomonas sp. HK-6 in Two Types of Media

Yun-Seok Cho; Bheong-Uk Lee; Hyung-Yeel Kahng; Kye-Heon Oh

doi:10.1007/s12275-008-0108-0

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Research Support, Non-U.S. Gov't
Comparative Analysis of 2,4,6-Trinitrotoluene (TNT)-Induced Cellular Responses and Proteomes in Pseudomonas sp. HK-6 in Two Types of Media: Yun-Seok Cho ¹, Bheong-Uk Lee ², Hyung-Yeel Kahng ³, Kye-Heon Oh ¹; Journal of Microbiology 2009;47(2):220-224.
DOI: https://doi.org/10.1007/s12275-008-0108-0
Published online: May 2, 2009

¹Department of Biotechnology, Soonchunhyang University, Asan 336-600, Republic of Korea, ²Division of Biological Sciences, Kosin University, Busan 606-701, Republic of Korea, ³Department of Environmental Education, Sunchon National University, Suncheon 540-742, Republic of Korea

Corresponding author: Kye-Heon Oh , Tel: 82-41-530-1353,

Received: 28 April 2008 • Accepted: 8 December 2008

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Abstract

TNT-induced cellular responses and proteomes in Pseudomonas sp. HK-6 were comparatively analyzed in two different media: basal salts (BS) and Luria broth (LB). HK-6 cells could not degrade more than 0.5 mM TNT with BS medium, while in LB medium, they exhibited the enhanced capability to degrade as much as 3.0 mM TNT. Analysis of total cellular fatty acids in HK-6 cells suggested that the relative abundance of several saturated or unsaturated fatty acids is altered under TNT-mediated stress conditions. Scanning electron microscopy showed the presence of perforations, irregular rod formations, and wrinkled extracellular surfaces in cells under TNT stress. Proteomic analysis of soluble protein fractions from HK-6
cultures grown with TNT as a substrate revealed 11 protein spots induced by TNT. Among these, seven proteins (including Alg8, AlgB, NirB, and the AhpC/Tsa family) were detected only in LB medium containing TNT. The proteins AspS, Tsf, and assimilatory nitrate reductase were increasingly expressed only in BS medium containing TNT. The protein dGTPase was found to be induced and expressed when cells were grown in either type of TNT-containing media. These results provide a better understanding of the cytotoxicity and survival mechanism used by Pseudomonas sp. HK-6 when placed under TNT stress conditions.

Keywords: cellular responses;proteomics;alginate;Pseudomonas sp. HK-6;6-trinitrotoluene

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Comparative Analysis of 2,4,6-Trinitrotoluene (TNT)-Induced Cellular Responses and Proteomes in Pseudomonas sp. HK-6 in Two Types of Media

J. Microbiol. 2009;47(2):220-224. Published online May 2, 2009

DOI: https://doi.org/10.1007/s12275-008-0108-0

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