Characterization of a Baculovirus Newly Isolated from the Tea Slug Moth, Iragoidae fasciata

Li-Rong Yang; Xiao Qiang; Bao-Qin Zhang; Mei-Jun Tang; Chuan-Xi Zhang

doi:10.1007/s12275-008-0253-5

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Research Support, Non-U.S. Gov't
Characterization of a Baculovirus Newly Isolated from the Tea Slug Moth, Iragoidae fasciata: Li-Rong Yang ^1,2, Xiao Qiang ³, Bao-Qin Zhang ¹, Mei-Jun Tang ³, Chuan-Xi Zhang ¹; Journal of Microbiology 2009;47(2):208-213
DOI: https://doi.org/10.1007/s12275-008-0253-5
Published online: May 2, 2009

¹Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, P. R. China, ²Henan Academy of Agricultural Sciences, Zhengzhou 450002, P. R. China, ³Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, Zhejiang 310000, P. R. China¹Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, P. R. China, ²Henan Academy of Agricultural Sciences, Zhengzhou 450002, P. R. China, ³Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, Zhejiang 310000, P. R. China

Corresponding author: Chuan-Xi Zhang , Tel: 86-571-8697-1697,

Received: 13 October 2008 • Accepted: 23 December 2008

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Abstract

The tea slug moth Iragoidae fasciata (Lepidoptera, Eucleidae) is one of the main insect pests that attack tea bushes. A new nucleopolyhedrovirus (NPV) called Iragoidae fasciata NPV (IrfaNPV) was recently isolated from diseased larvae. An 11,626 bp fragment of the viral genomic DNA containing the polyhedrin gene and other 12 genes was cloned and sequenced. Gene comparison and phylogenetic analysis showed that IrfaNPV is a member of the Group I NPVs. However, the genomic organization of IrfaNPV is highly distinct. In addition, electron microscopy analysis showed that IrfaNPV is a single nucleocapsid NPV (SNPV). An inoculation assay showed that IrfaNPV is semi-permissive in the Trichoplusia ni cell line Tn-5B1-4. Bioassays on lethal concentration (LC50) and lethal time (LT50) were conducted to test the susceptibility of I. fasciata larvae to the virus.

Keywords: Iragoidae fasciata; nucleopolyhedrovirus; SNPV; polyhedrin region; phylogeny; permissive cell line

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Characterization of a Baculovirus Newly Isolated from the Tea Slug Moth, Iragoidae fasciata

J. Microbiol. 2009;47(2):208-213. Published online May 2, 2009

DOI: https://doi.org/10.1007/s12275-008-0253-5

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