Phylogeny of a Novel “Helicobacter heilmannii” Organism from a Japanese Patient with Chronic Gastritis Based on DNA Sequence Analysis of 16S rRNA and Urease Genes

Takehisa Matsumoto; Masatomo Kawakubo; Mayumi Shiohara; Toshiko Kumagai; Eiko Hidaka; Kazuyoshi Yamauchi; Kozue Oana; Kenji Matsuzawa; Hiroyoshi Ota; Yoshiyuki Kawakami

doi:10.1007/s12275-008-0313-x

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Phylogeny of a Novel “Helicobacter heilmannii” Organism from a Japanese Patient with Chronic Gastritis Based on DNA Sequence Analysis of 16S rRNA and Urease Genes: Takehisa Matsumoto ^1,2, Masatomo Kawakubo ², Mayumi Shiohara ^1,2,3, Toshiko Kumagai ², Eiko Hidaka ², Kazuyoshi Yamauchi ², Kozue Oana ⁴, Kenji Matsuzawa ⁵, Hiroyoshi Ota ⁴, Yoshiyuki Kawakami ⁴; Journal of Microbiology 2009;47(2):201-207
DOI: https://doi.org/10.1007/s12275-008-0313-x
Published online: May 2, 2009

¹Department of Laboratory Medicine, Shinshu University Graduate School of Medicine, Asahi, Matsumoto 390-8621, Japan, ²Department of Laboratory Medicine, Shinshu University Hospital, Asahi, Matsumoto 390-8621, Japan, ³Department of Nursing, Shinshu University Hospital, Asahi, Matsumoto 390-8621, Japan, ⁴Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, Asahi, Matsumoto 390-8621, Japan, ⁵Maruko General Hospital, Maruko, Ueda 386-0493, Japan¹Department of Laboratory Medicine, Shinshu University Graduate School of Medicine, Asahi, Matsumoto 390-8621, Japan, ²Department of Laboratory Medicine, Shinshu University Hospital, Asahi, Matsumoto 390-8621, Japan, ³Department of Nursing, Shinshu University Hospital, Asahi, Matsumoto 390-8621, Japan, ⁴Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, Asahi, Matsumoto 390-8621, Japan, ⁵Maruko General Hospital, Maruko, Ueda 386-0493, Japan

Corresponding author: Yoshiyuki Kawakami , Tel: 81-263-37-2381,

Received: 15 December 2008 • Accepted: 28 January 2009

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Abstract

“Helicobacter heilmannii” is an uncultivable spiral-shaped bacterium inhabiting the human gastric mucosa. It is larger and more tightly-coiled than H. pylori. We encountered a patient with chronic gastritis infected a “H. heilmannii”-like organism (HHLO), designated as SH6. Gastric mucosa derived from the patient was orally ingested by specific pathogen free mice. Colonization of the mice by SH6 was confirmed by electron microscopy of gastric tissue specimens. In an attempt to characterize SH6, 16S rRNA and urease genes were sequenced. The 16S rRNA gene sequence was most similar (99.4%; 1,437/1,445 bp) to HHLO C4E from a cheetah. However, the urease gene sequence displayed low similarity (81.7%; 1,240/1,516 bp) with HHLO C4E. Taxonomic analysis disclosed that SH6 represents a novel strain and should constitute a novel taxon in the phylogenetic trees, being discriminated from any other taxon, with the ability of infecting human gastric mucosa.

Keywords: “Helicobacter heilmannii”; HHLO; phylogeny; phylogenetic tree; 16S rRNA; urease gene

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Phylogeny of a Novel “Helicobacter heilmannii” Organism from a Japanese Patient with Chronic Gastritis Based on DNA Sequence Analysis of 16S rRNA and Urease Genes

J. Microbiol. 2009;47(2):201-207. Published online May 2, 2009

DOI: https://doi.org/10.1007/s12275-008-0313-x

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