Translocation of Green Fluorescent Protein to Cyanobacterial Periplasm Using Ice Nucleation Protein

Wipa Chungjatupornchai; Sirirat Fa-aroonsawat

doi:10.1007/s12275-008-0188-x

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Research Support, Non-U.S. Gov't
Translocation of Green Fluorescent Protein to Cyanobacterial Periplasm Using Ice Nucleation Protein: Wipa Chungjatupornchai , Sirirat Fa-aroonsawat; Journal of Microbiology 2009;47(2):187-192.
DOI: https://doi.org/10.1007/s12275-008-0188-x
Published online: May 2, 2009

Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakornpathom 73170, ThailandInstitute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakornpathom 73170, Thailand

Corresponding author: Wipa Chungjatupornchai , Tel: 66-2-800-2732,

Received: 4 August 2008 • Accepted: 7 December 2008

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Abstract

The translocation of proteins to cyanobacterial cell envelope is made complex by the presence of a highly differentiated membrane system. To investigate the protein translocation in cyanobacterium Synechococcus PCC 7942 using the truncated ice nucleation protein (InpNC) from Pseudomonas syringae KCTC 1832, the green fluorescent protein (GFP) was fused in frame to the carboxyl-terminus of InpNC. The fluorescence of GFP was found almost entirely as a halo in the outer regions of cells which appeared to correspond to the periplasm as demonstrated by confocal laser scanning microscopy, however, GFP was not displayed on the outermost cell surface. Western blotting analysis revealed that InpNC-GFP fusion protein was partially degraded. The N-terminal domain of InpNC may be susceptible to protease attack; the remaining C-terminal domain conjugated with GFP lost the ability to direct translocation across outer membrane and to act as a surface display motif. The fluorescence intensity of cells with periplasmic GFP was approximately 6-fold lower than that of cells with cytoplasmic GFP. The successful translocation of the active GFP to the periplasm may provide a potential means to study the property of cyanobacterial periplasmic substances in response to environmental changes in a non-invasive manner.

Keywords: cyanobacteria;Synechococcus;translocation;periplasm;ice nucleation protein;green fluorescent

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Translocation of Green Fluorescent Protein to Cyanobacterial Periplasm Using Ice Nucleation Protein

J. Microbiol. 2009;47(2):187-192. Published online May 2, 2009

DOI: https://doi.org/10.1007/s12275-008-0188-x

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