Intracellular Substrates of a Heme-Containing Ascorbate Oxidase in Pleurotus ostreatus

Seung-Rock Lee; Woo-Jeong Joo; Yong-Un Baek; Youn-Kyong Lee; Seong-Woon Yu; Yeon-Ran Kim; Kee-Oh Chay; Seung-Hyun Cho; Sa-Ouk Kang

doi:10.1007/s12275-008-0307-8

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Research Support, Non-U.S. Gov't
Intracellular Substrates of a Heme-Containing Ascorbate Oxidase in Pleurotus ostreatus: Seung-Rock Lee ¹, Woo-Jeong Joo ², Yong-Un Baek ², Youn-Kyong Lee ², Seong-Woon Yu ², Yeon-Ran Kim ², Kee-Oh Chay ¹, Seung-Hyun Cho ², Sa-Ouk Kang ²; Journal of Microbiology 2009;47(2):178-186.
DOI: https://doi.org/10.1007/s12275-008-0307-8
Published online: May 2, 2009

¹Department of Biochemistry, Chonnam National University Medical School, Gwangju 501-190, Republic of Korea, ²Laboratory of Biophysics, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea

Corresponding author: Seung-Rock Lee , Tel: 82-2-880-6703; 82-62-220-4108,
Sa-Ouk Kang , Tel: 82-2-880-6703; 82-62-220-4108,

Received: 19 December 2008 • Accepted: 12 February 2009

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Abstract

A novel heme-containing ascorbate oxidase isolated from oyster mushroom, Pleurotus ostreatus, catalyzes oxidation of ascorbic acid (Kim et al., 1996). In this report, we describe the identification of intracellular substrates of the enzyme in the mushroom. Six compounds, which can serve as substrate of the heme-containing ascorbate oxidase, were identified as L-ascorbic acid, D-erythroascorbic acid, 5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, 5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid, 5-methyl-5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, and 5-methyl-5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid. All of the compounds were oxidized at a significant rate by the heme-containing ascorbate oxidase. Oxidation of the compounds produced equimolar amounts of hydrogen peroxide per mole of substrate.

Keywords: ascorbate oxidase;substrates;Pleurotus ostreatus

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Intracellular Substrates of a Heme-Containing Ascorbate Oxidase in Pleurotus ostreatus

J. Microbiol. 2009;47(2):178-186. Published online May 2, 2009

DOI: https://doi.org/10.1007/s12275-008-0307-8

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