Mobilization Functions of the Bacteriocinogenic Plasmid pRJ6 of Staphylococcus aureus

Marcus Livio Varella Coelho; Hilana Ceotto; Danielle Jannuzzi Madureira; Ingolf F. Nes; Maria do Carmo de Freire Bastos

doi:10.1007/s12275-009-0044-7

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Mobilization Functions of the Bacteriocinogenic Plasmid pRJ6 of Staphylococcus aureus: Marcus Livio Varella Coelho ¹, Hilana Ceotto ², Danielle Jannuzzi Madureira ², Ingolf F. Nes ³, Maria do Carmo de Freire Bastos ^1,2; Journal of Microbiology 2009;47(3):327-336.
DOI: https://doi.org/10.1007/s12275-009-0044-7
Published online: June 26, 2009

¹Departamento de Genetica, Instituto de Biologia, Norwegian University of Life Sciences, As N1432, Norway, ²Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Goes, Norwegian University of Life Sciences, As N1432, Norway, ³UFRJ, Rio de Janeiro, Brasil, and Laboratory of Microbial Gene Technology, Norwegian University of Life Sciences, As N1432, Norway

Corresponding author: Maria do Carmo de Freire Bastos , Tel: 55-21-2560-4344,

Received: 12 February 2009 • Accepted: 24 March 2009

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Abstract

Plasmid pRJ6 is the first known bacteriocinogenic mobilizable (Mob) plasmid of Staphylococcus aureus. Its Mob region is composed of four mob genes (mobCDAB) arranged as an operon, a genetic organization uncommon among S. aureus Mob plasmids. oriTpRJ6 was detected in a region of 431 bp, positioned immediately upstream of mobC. This region, when cloned into pCN37, was able to confer mobilization to the recombinant plasmid only in the presence of pRJ6. The entire Mob region, including oriTpRJ6, is much more similar to Mob regions from several coagulase-negative staphylococci plasmids, although some remarkable similarities with S. aureus Mob plasmids can also be noted. These similarities include the presence within oriTpRJ6 of the three mcb (MobC binding sites), firstly described in pC221 and pC223, an identical nick site also found in these same plasmids, and a nearly identical srapC223 site (sequence recognized by MobA). pRJ6 was successfully transferred to S. epidermidis by conjugation in the presence of the conjugative plasmid pGO1. Altogether these findings suggest that pRJ6 might have been originally a coagulase-negative staphylococci plasmid that had been transferred successfully to S. aureus.

Keywords: mobilization;bacteriocin;oriT;Staphylococcus;plasmid pRJ6;conjugal transfer

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Mobilization Functions of the Bacteriocinogenic Plasmid pRJ6 of Staphylococcus aureus

J. Microbiol. 2009;47(3):327-336. Published online June 26, 2009

DOI: https://doi.org/10.1007/s12275-009-0044-7

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