The Three-Dimensional Morphology of Candida albicans as Seen by High-Resolution Scanning Electron Microscopy

Michela Isola; Raffaella Isola; Maria Serenella Lantini; Alessandro Riva

doi:10.1007/s12275-008-0212-1

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Research Support, Non-U.S. Gov't
The Three-Dimensional Morphology of Candida albicans as Seen by High-Resolution Scanning Electron Microscopy: Michela Isola , Raffaella Isola , Maria Serenella Lantini , Alessandro Riva; Journal of Microbiology 2009;47(3):260-264.
DOI: https://doi.org/10.1007/s12275-008-0212-1
Published online: June 26, 2009

Department of Cytomorphology, Cittadella Universitaria di Monserrato, University of Cagliari, Monserrato 09042, ItalyDepartment of Cytomorphology, Cittadella Universitaria di Monserrato, University of Cagliari, Monserrato 09042, Italy

Corresponding author: Raffaella Isola , Tel: 39-070-675-4041,

Received: 1 September 2008 • Accepted: 24 February 2009

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Abstract

The fine structure of Candida albicans has been repeatedly described by transmission electron microscopy, whereas studies by high-resolution scanning electron microscopy (HRSEM) are rare and devoted solely to the study of its external morphology. This report describes the results of an HRSEM study on C. albicans carried out by an osmium maceration protocol modified to better retain the structural characteristics of this yeast. Thus, we visualized various intracellular structures including invaginations of cell membrane (plasmalemmasomes), nuclear envelope, mitochondria, the vacuolar system, and two additional structures that might represent a form of endoplasmic reticulum and the Golgi apparatus. The present investigation, which for the first time shows the organelles of C. albicans at the 3D level, may lead to a better understanding of its cell physiology.

Keywords: high-resolution scanning electron microscopy;osmium maceration method;Candida albicans

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The Three-Dimensional Morphology of Candida albicans as Seen by High-Resolution Scanning Electron Microscopy

J. Microbiol. 2009;47(3):260-264. Published online June 26, 2009

DOI: https://doi.org/10.1007/s12275-008-0212-1

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