- Volume 52(12); December 2014
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Review
- Minireivew] Protective Role of Gut Commensal Microbes against Intestinal Infections
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My Young Yoon , Keehoon Lee , Sang Sun Yoon
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J. Microbiol. 2014;52(12):983-989. Published online November 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4655-2
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Abstract
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The human gastrointestinal tract is colonized by multitudes
of microorganisms that exert beneficial effects on human
health. Mounting evidence suggests that intestinal microbiota
contributes to host resistance against enteropathogenic
bacterial infection. However, molecular details that account
for such an important role has just begun to be understood.
The commensal microbes in the intestine regulate gut homeostasis
through activating the development of host innate
immunity and producing molecules with antimicrobial activities
that directly inhibit propagation of pathogenic bacteria.
Understanding the protective roles of gut microbiota
will provide a better insight into the molecular basis that underlies
complicated interaction among host-pathogen-symbiont.
In this review, we highlighted recent findings that help
us broaden our knowledge of the intestinal ecosystem and
thereby come up with a better strategy for combating enteropathogenic
infection.
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Journal Article
- Nocardioides paucivorans sp. nov. Isolated from Soil
-
Jae-Hyung Ahn , Jun-Muk Lim , Soo-Jin Kim , Jaekyeong Song , Soon-Wo Kwon , Hang-Yeon Weon
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J. Microbiol. 2014;52(12):990-994. Published online November 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4587-x
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43
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10
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Abstract
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One strain, designated KIS31-44T, was isolated from a soil sample collected from Dokdo Island, South Korea. The strain is Gram-stain-positive, aerobic, non-spore-forming and nonmotile. It grows optimally at 28–30°C, at pH 7.0 and 0% NaCl. 16S rRNA gene sequence analysis showed that strain KIS31-44T belonged to the genus Nocardioides and shared the highest sequence similarities with Nocardioides aestuarii JC2056T (95.5%) and Nocardioides terrae VA15T (95.0%). The major fatty acids of strain KIS31-44T were C17:1 ω6c, C18:1 ω9c, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), iso-C16:0, C18:0 10-methyl (TBSA), C16:0 2-OH, C17:0 10-methyl, and iso-C16:1 H. The major isoprenoid quinone was MK-8 (H4). The strain
contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The peptidoglycan structure was A3γ-type with LL-diaminopimelic acid. Based on these data, the isolate represents one novel species
in the genus Nocardioides, for which the name Nocardioides paucivorans sp. nov. (type strain KIS31-44T =DSM 27142T =KACC 17309T) is proposed.
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Nocardioides alcanivorans sp. nov., a novel hexadecane-degrading species isolated from plastic waste
Julia Mitzscherling, Joana MacLean, Daniel Lipus, Alexander Bartholomäus, Kai Mangelsdorf, André Lipski, Vladimir Roddatis, Susanne Liebner, Dirk Wagner
International Journal of Systematic and Evolutionary Microbiology
.2022;[Epub] CrossRef -
Nocardioides sambongensis sp. nov., isolated from Dokdo Islands soil
Ye-Ji Hwang, Jin-Soo Son, Soo-Yeong Lee, Yuxi He, YoungJae Jo, Jae-Ho Shin, Sa-Youl Ghim
International Journal of Systematic and Evolutionary Microbiology
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Bao-Zhu Fang, Ming-Xian Han, Jian-Yu Jiao, Xiao-Tong Zhang, Yuan-Guo Xie, Wael N. Hozzein, Dalal Hussien M. Alkhalifah, Min Xiao, Wen-Jun Li
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Israel Journal of Ecology and Evolution.2018; 64(1-4): 1. CrossRef -
Nocardioides cavernae sp. nov., an actinobacterium isolated from a karst cave
Ming-Xian Han, Bao-Zhu Fang, Ye Tian, Wan-Qin Zhang, Jian-Yu Jiao, Lan Liu, Zi-Tong Zhang, Min Xiao, Da-Qiao Wei, Wen-Jun Li
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Nocardioides thalensis sp. nov., isolated from a desert
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International Journal of Systematic and Evolutionary Microbiology
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Nocardioides ungokensis sp. nov., isolated from lake sediment
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Research Support, Non-U.S. Gov'ts
- Effect of Long-Term Different Fertilization on Bacterial Community Structures and Diversity in Citrus Orchard Soil of Volcanic Ash
-
Jae Ho Joa , Hang Yeon Weon , Hae Nam Hyun , Young Chull Jeun , Sang Wook Koh
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J. Microbiol. 2014;52(12):995-1001. Published online November 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4129-6
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46
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27
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Abstract
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This study was conducted to assess bacterial species richness,
diversity and community distribution according to different
fertilization regimes for 16 years in citrus orchard soil of volcanic
ash. Soil samples were collected and analyzed from
Compost (cattle manure, 2,000 kg/10a), 1/2 NPK+compost
(14-20-14+2,000 kg/10a), NPK+compost (28-40-28+2,000
kg/10a), NPK (28-40-28 kg/10a), 3 NPK (84-120-84 kg/10a),
and Control (no fertilization) plot which have been managed
in the same manners with compost and different amount of
chemical fertilization. The range of pyrosequencing reads
and OTUs were 4,687–7,330 and 1,790–3,695, respectively.
Species richness estimates such as Ace, Chao1, and Shannon
index were higher in 1/2 NPK+compost than other treatments,
which were 15,202, 9,112, 7.7, respectively. Dominant
bacterial groups at level of phylum were Proteobacteria, Acidobacteria,
and Actinobacteria. Those were occupied at 70.9%
in 1/2 NPK+compost. Dominant bacterial groups at level
of genus were Pseudolabrys, Bradyrhizobium, and Acidobacteria.
Those were distributed at 14.4% of a total of bacteria
in Compost. Soil pH displayed significantly closely related
to bacterial species richness estimates such as Ace, Chao1
(p<0.05) and Shannon index (p<0.01). However, it showed
the negative correlation with exchangeable aluminum contents
(p<0.05). In conclusion, diversity of bacterial community
in citrus orchard soil was affected by fertilization management,
soil pH changes and characteristics of volcanic ash.
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Vishal Shah, Sanjana Subramaniam
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Lixia Xu, Min Yi, Huilan Yi, Erhu Guo, Aiying Zhang
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Jinu Eo, Kee-Choon Park
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- Effect of Zinc on Growth Performance, Gut Morphometry, and Cecal Microbial Community in Broilers Challenged with Salmonella enterica serovar Typhimurium
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Yuxin Shao , Zhao Lei , Jianmin Yuan , Ying Yang , Yuming Guo , Bingkun Zhang
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J. Microbiol. 2014;52(12):1002-1011. Published online November 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4347-y
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54
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65
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Abstract
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To evaluate the effects of supplemental zinc on growth performance,
gut morphometry, and the cecal microbial community
in broilers challenged with Salmonella typhimurium,
180, 1-day-old male Cobb 500 broiler chicks were randomly
assigned to 3 treatments with ten replicates for a 42 day experiment.
The 3 treatments were: unchallenged, S. typhimurium-
challenged, and S. typhimurium-challenged with 120
mg/kg of zinc supplementation in the diet. Salmonella infection
caused a reduction in body-weight gain and feed intake,
disrupted the intestinal structure by decreasing the
villus-height/crypt-depth ratio of the ileum and increasing
the apoptotic index of ileal epithelial cells. Moreover, the
cecal microbial community was altered by Salmonella infection,
as demonstrated by a reduced number of Lactobacillus
and total bacteria. Dietary zinc supplementation improved
growth performance by increasing the body-weight gain
and feed intake in the challenged broilers. In addition, zinc
repaired intestinal injury by reducing the apoptotic index of
ileal epithelial cells, enhancing villus height and the villusheight/
crypt-depth ratio of the ileum, and the proliferation
index of ileal epithelial cells. Finally, zinc regulated the cecal
microbial community by increasing the number of total bacteria
and beneficial Lactobacillus bacteria, and reducing the
number of Salmonella. The results indicated that dietary
zinc supplementation improved growth performance, intestinal
morphology, and intestinal microbiota in S. typhimurium-
challenged broilers.
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- Transformation of Inorganic P Fractions of Soil and Plant Growth Promotion by Phosphate-solubilizing Ability of Penicillium oxalicum I1
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Mingbo Gong , Peng Du , Xue Liu , Changxiong Zhu
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J. Microbiol. 2014;52(12):1012-1019. Published online November 3, 2014
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DOI: https://doi.org/10.1007/s12275-014-4406-4
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45
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10
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Abstract
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The solubilization of tricalcium phosphate is often considered
as the standard for screening of most phosphate-solubilizing
microorganisms (PSMs). However, usually the effect
of large-scale application of PSM on the promotion of crop
growth varies. This study presents an efficient method for
screening and testing phosphate-solubilizing fungus that
enhance plant growth. A fungus Penicillium oxalicum I1 (PI1)
was isolated and identified that had high ability of phosphate-
solubilization and could utilize maize root exudates as
sources, and propagate well in vitro and in soil. P-I1 excreted
oxalic acid and reached 593.9 μg/ml, and the pH value was
decreased from 6.90 to 1.65 in 26 h. The amount of P-I1 increased
by 48-fold in 28 d and was maintained for 49 d in
soil. PSM showed selectivity on the transformation of the
different forms of phosphorus, a wide range of insoluble
phosphates, such as Ca8H2(PO4)6·5H2O, AlPO4, FePO4, and
Ca10(PO4)6(OH)2, were converted to soluble CaHPO4 in soil,
and CaHPO4 was also inhibited from being converted into
insoluble phosphate by P-I1. The Ca2-P content reached 27.11
μg/g soil on day 28 at 20°C, which increased by 110.32%,
and plant growth promotion was tested and verified, the
results
showed that maize yield increased remarkably than
control after inoculated P-I1, maize yield increased maximum
by 14.47%. The data presented that P-I1 appear attractive
for exploring their plant growth-promoting activity
and potential field application.
-
Citations
Citations to this article as recorded by

- Inoculations of phosphate-solubilizing bacteria alter soil microbial community and improve phosphorus bioavailability for moso bamboo (Phyllostachys edulis) growth
Yaohui Liu, Ashrafun Nessa, Qiyuan Zheng, Dongnan Hu, Wenyuan Zhang, Manyun Zhang
Applied Soil Ecology.2023; 189: 104911. CrossRef - Biological Control Potential of Endophytic Fungi with Amelioration of Systemic Resistance in Sunflower and GC–MS Metabolic Profiling of Talaromyces assiutensis
Hafiza Farhat, Faizah Urooj, Muhammed Irfan, Nida Sohail, Saima Majeed, Shahid Ullah, Hafza Asma Shafique
Current Microbiology.2023;[Epub] CrossRef - Effect of Bacillus megaterium var. phosphaticum applied together with rock phosphate on wheat yield and some soil properties in a calcareous soil
Betül BAYRAKLI
EURASIAN JOURNAL OF SOIL SCIENCE (EJSS).2022; 11(3): 198. CrossRef - Plant Growth Promoting Filamentous Fungi and Their Application in the Fertilization of Pastures for Animal Consumption
Rosalba Argumedo-Delira, Mario J. Gómez-Martínez, Jairo Mora-Delgado
Agronomy.2022; 12(12): 3033. CrossRef - The fungal and archaeal community within plant rhizosphere: a review on their contribution to crop safety
Saheed Adekunle Akinola, Olubukola Oluranti Babalola
Journal of Plant Nutrition.2021; 44(4): 600. CrossRef - A comprehensive synthesis unveils the mysteries of phosphate‐solubilizing microbes
Jin‐tian Li, Jing‐li Lu, Hong‐yu Wang, Zhou Fang, Xiao‐juan Wang, Shi‐wei Feng, Zhang Wang, Ting Yuan, Sheng‐chang Zhang, Shu‐ning Ou, Xiao‐dan Yang, Zhuo‐hui Wu, Xiang‐deng Du, Ling‐yun Tang, Bin Liao, Wen‐sheng Shu, Pu Jia, Jie‐Liang Liang
Biological Reviews.2021; 96(6): 2771. CrossRef - Effect of Acid Production by Penicillium oxalicum on Physicochemical Properties of Bauxite Residue
Yifan Zhang, Rui Xue, Xuan He, Qingyu Cheng, William Hartley, Shengguo Xue
Geomicrobiology Journal.2020; 37(10): 929. CrossRef - Sustainable Chemistry: Solubilization of Phosphorus from Insoluble Phosphate Material Hydroxyapatite with Ozonized Biochar
Oumar Sacko, Rachel Whiteman, Gyanendra Kharel, Sandeep Kumar, James W. Lee
ACS Sustainable Chemistry & Engineering.2020; 8(18): 7068. CrossRef - Characterization and variation of the rhizosphere fungal community structure of cultivated tetraploid cotton
Qinghua Qiao, Jingxia Zhang, Changle Ma, Furong Wang, Yu Chen, Chuanyun Zhang, Hui Zhang, Jun Zhang, Vijai Gupta
PLOS ONE.2019; 14(10): e0207903. CrossRef - Gibberellins in Penicillium strains: Challenges for endophyte-plant host interactions under salinity stress
Ana Lúcia Leitão, Francisco J. Enguita
Microbiological Research.2016; 183: 8. CrossRef
- The Role of Wheat Germ Agglutinin in the Attachment of Pseudomonas sp. WS32 to Wheat Root
-
Jian Zhang , Liyuan Meng , Yuanyuan Cao , Huiping Chang , Zhongyou Ma , Leni Sun , Ming Zhang , Xinyun Tang
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J. Microbiol. 2014;52(12):1020-1024. Published online November 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4089-x
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57
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1
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Abstract
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Wheat germ agglutinin (WGA), which is secreted on the
surface of wheat root, has been defined as a protein that reversibly
and non-enzymatically binds to specific carbohydrates.
However, little attention has been paid to the function
of WGA in the attachment of bacteria to their host plants.
The aim of this study was to investigate the role of WGA in
the attachment of Pseudomonas sp. WS32 to wheat roots.
Wheat roots were initially treated with double-distilled water,
WGA-H (WGA solution that was heated at 100°C for 15 min)
and WGA, independently. Subsequently, the roots were coincubated
with cell solutions (109 cells/ml). A dilution plate
method
using a solid nutrient medium was employed to determine
the adsorption of WS32 to wheat roots. WGA was
labeled with fluorescein isothiocyanate and detected using
the fluorescent in situ hybridization (FISH) technique. The
number of adsorptive WS32 cells on wheat roots was significantly
increased when the wheat roots were pretreated
with WGA, compared with the control treatment (p = 0.01).
However, WGA-H failed to increase the amount of bacterial
cells that attached to the wheat roots because of the loss
of its physiological activity. The FISH assay also revealed
that more cells adhered to WGA-treated wheat roots than to
control or WGA-H-treated roots. The results indicated that
WGA can mediate Pseudomonas strain WS32’s adherence
to wheat seedling roots. The findings of this study provide a
better understanding of the processes involved in plant-microbe
interactions.
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Citations
Citations to this article as recorded by

- Isolation and purification of wheat germ agglutinin and analysis of its properties
Han Wang
IOP Conference Series: Earth and Environmental Science.2017; 100: 012021. CrossRef
- Mycoflora Dynamics Analysis of Korean Traditional Wheat-based Nuruk
-
Jyotiranjan Bal , Suk-Hyun Yun , Ha-Yeon Song , Soo-Hwan Yeo , Jae Hyun Kim , Jung-Mi Kim , Dae-Hyuk Kim
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J. Microbiol. 2014;52(12):1025-1029. Published online November 29, 2014
-
DOI: https://doi.org/10.1007/s12275-014-4620-0
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47
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24
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Abstract
-
The growing popularity of traditional Korean alcoholic beverages
has led to a demand for quality enhancement of the
traditional starter culture nuruk, which consists primarily
of wheat. Therefore, this study focused on mycoflora characterization
and the temporal variations in traditional wheatbased
nuruks fermented at two representative traditional temperature
conditions for 30 days. Nuruk A was fermented at a
constant temperature of 36°C for 30 days and nuruk B was
fermented at a high initial temperature of 45°C for 10 days
followed by 35°C for 20 days. The average mycoflora load in
the two different nuruk conditions did not vary significantly
between the 0 and 30 day cultures, and a maximum load of
8.39 log CFU/g was observed for nuruk A on culture day 3
and 7.87 log CFU/g for nuruk B on culture day 30. Within two
samples, pH was negatively correlated with temporal changes
in mycoflora load. The pH of nuruk A was significantly lower
than that of nuruk B at all of the time points evaluated. Culture-
dependent characterization led to the identification of
55 fungal isolates belonging to 9 genera and 15 species, with
the most prominent genera comprising Lichtheimia, Penicillium,
Trametes, Aspergillus, Rhizomucor, and Mucor. A total
of 25 yeast isolates were characterized belonging to 6 genera
and 7 species, the most prominent among which were Rhodotorula,
Pichia, Debaryomyces, Saccharomycopsis, and Torulospora.
Mycofloral community dynamics analysis revealed
that both samples A and B varied considerably with respect
to the fungal communities over a span of 30 days.
-
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Eun Hee Park, Eun Bi Jeon, Pantu Kumar Roy, Sung-Hee Park, Shin Young Park
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Kevin Scaife, Trung D. Vo, Yvonne Dommels, Elisa Leune, Kaj Albermann, Lucie Pařenicová
Food and Chemical Toxicology.2023; 179: 113972. CrossRef - Environmental factors drive microbial succession and huangjiu flavor formation during raw wheat qu fermentation
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Food Bioscience.2023; 51: 102342. CrossRef - Characterisation of Korean rice wine (makgeolli) prepared by different processing methods
Barry Wong, Kevin Muchangi, Edward Quach, Tony Chen, Adrian Owens, Don Otter, Megan Phillips, Rothman Kam
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nuruk
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Korean Journal of Food Preservation.2022; 29(1): 105. CrossRef - Amylolytic Fungi in the Ethnic Beer Starter “emao” and Their Beer-Producing Attributes
Nitesh Boro, Diganta Narzary
Frontiers in Sustainable Food Systems.2022;[Epub] CrossRef - Enzyme activity of Aspergillus section Nigri strains isolated from the Korean fermentation starter, nuruk
Eunji Jeong, Jeong-Ah Seo
Journal of Microbiology.2022; 60(10): 998. CrossRef - Molecular phylogeny and morphological characterization of the aetiological agent of sour rot on fruits and vegetables in Brazil
Simone Albino Paes, André Wilson Campos Rosado, Ailton Reis, Olinto Liparini Pereira
Plant Pathology.2022; 71(2): 386. CrossRef - pH Changes Have a Profound Effect on Gene Expression, Hydrolytic Enzyme Production, and Dimorphism in Saccharomycopsis fibuligera
Mohamed El-Agamy Farh, Najib Abdellaoui, Jeong-Ah Seo
Frontiers in Microbiology.2021;[Epub] CrossRef - Aspergillus oryzae-Fermented Wheat Peptone Enhances the Potential of Proliferation and Hydration of Human Keratinocytes through Activation of p44/42 MAPK
Kyung Man Hahm, See-Hyoung Park, Sae Woong Oh, Ji Hye Kim, Hyun Sook Yeom, Hye Ja Lee, Seoyeon Yang, Jae Youl Cho, Jin Oh Park, Jongsung Lee
Molecules.2021; 26(19): 6074. CrossRef - Community structure and metabolic potentials of the traditional rice beer starter ‘emao’
Diganta Narzary, Nitesh Boro, Ashis Borah, Takashi Okubo, Hideto Takami
Scientific Reports.2021;[Epub] CrossRef - Omics in traditional vegetable fermented foods and beverages
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Yi Fan, Xiaoning Huang, Jingyu Chen, Beizhong Han
Frontiers in Microbiology.2020;[Epub] CrossRef - Natural Occurrence of Aflatoxigenic Aspergillus Species and Aflatoxins in Traditional Korean Fermentation Starters, Meju and Nuruk
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Journal of Food Hygiene and Safety.2020; 35(5): 438. CrossRef - Effects of initial moisture content of Korean traditional wheat-based fermentation starter nuruk on microbial abundance and diversity
Jyotiranjan Bal, Suk-Hyun Yun, Soo-Hwan Yeo, Jung-Mi Kim, Beom-Tae Kim, Dae-Hyuk Kim
Applied Microbiology and Biotechnology.2017; 101(5): 2093. CrossRef - Comparative analysis of nonvolatile and volatile metabolites in Lichtheimia ramosa cultivated in different growth media
Hyun Chung, NaKyeom Lee, Jeong-Ah Seo, Young-Suk Kim
Bioscience, Biotechnology, and Biochemistry.2017; 81(3): 565. CrossRef - Comprehensive analysis of fungal diversity and enzyme activity in nuruk, a Korean fermenting starter, for acquiring useful fungi
Emily Carroll, Tran Ngoc Trinh, Hokyoung Son, Yin-Won Lee, Jeong-Ah Seo
Journal of Microbiology.2017; 55(5): 357. CrossRef - A diversity study of Saccharomycopsis fibuligera in rice wine starter nuruk, reveals the evolutionary process associated with its interspecies hybrid
Mohamed El-Agamy Farh, Yunjoo Cho, Jae Yun Lim, Jeong-Ah Seo
Journal of Microbiology.2017; 55(5): 337. CrossRef - Whole-genome de novo sequencing, combined with RNA-Seq analysis, reveals unique genome and physiological features of the amylolytic yeast Saccharomycopsis fibuligera and its interspecies hybrid
Jin Ho Choo, Chang Pyo Hong, Jae Yun Lim, Jeong-Ah Seo, Young-Suk Kim, Dong Wook Lee, Sin-Gi Park, Gir Won Lee, Emily Carroll, Yin-Won Lee, Hyun Ah Kang
Biotechnology for Biofuels.2016;[Epub] CrossRef - Isolation and Identification of Yeasts from Jeju Island Soils
The Korean Journal of Mycology.2015;[Epub] CrossRef - Pyrosequencing reveals bacterial diversity in Korean traditional wheat-based nuruk
Jyotiranjan Bal, Suk-Hyun Yun, Myoung-Suk Choi, Soo-Hwan Yeo, Jung-Mi Kim, Dae-Hyuk Kim
Journal of Microbiology.2015; 53(12): 812. CrossRef
- Proteomic and Functional Analyses of a Novel Porin-like Protein in Xanthomonas oryzae pv. oryzae
-
Hye-Jee Park , Sang-Won Lee , Sang-Wook Han
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J. Microbiol. 2014;52(12):1030-1035. Published online November 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4442-0
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Abstract
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Proteomic analysis is a useful technique for postulating and elucidating protein functions. In the present work, a shotgun proteomic analysis was used to identify functions of the PXO_03968 gene (previously known as the ax21) from Xanthomonas oryzae pv. oryzae (Xoo), a causal agent for bacterial blight disease in rice. Structural prediction performed on the protein sequence encoded by PXO_03968 reveals that it encodes a putative porin-like protein, possessing a β-barrel domain with 10 β-strands and a signal peptide at the Nterminus. We renamed the gene as an omp1X (outer membrane protein 1 in Xoo), generated its knock out mutant (XooΔomp1X), and compared the protein expression level in the mutant to that in the wild type. A total of 106 proteins displayed more than 1.5-fold difference in expression between the mutant and the wild type strains. COG analysis revealed that these proteins are involved in cell motility as well as signal transduction. In addition, phenotypic analysis demonstrated that motility and biofilm formation in XooΔomp1X are lower than the wild type. These results provide new insights into the functions of outer membrane proteins in Gram-negative bacteria.
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Citations
Citations to this article as recorded by

- Stenotrophomonas maltophilia virulence: a current view
Vladimir Mikhailovich, Rustam Heydarov, Danila Zimenkov, Igor Chebotar
Frontiers in Microbiology.2024;[Epub] CrossRef - Molecular-genetic portrait of virulence of Stenotrophomonas maltophilia
Vladimir M. Mikhailovich, Rustam N. Geydarov, Julia A. Bocharova, Igor V. Chebotar
Journal of microbiology, epidemiology and immunobiology.2023; 100(5): 380. CrossRef - p‐Aminobenzoic acid inhibits the growth of soybean pathogen Xanthomonas axonopodis pv. glycines by altering outer membrane integrity
Yong‐Hui Jiang, Ting Liu, Xin‐Chi Shi, Daniela D Herrera‐Balandrano, Mei‐Ting Xu, Su‐Yan Wang, Pedro Laborda
Pest Management Science.2023; 79(10): 4083. CrossRef - Expression and function of clpS and clpA in Xanthomonas campestris pv. campestris
Hsueh-Hsia Lo, Hsiao-Ching Chang, Chao-Tsai Liao, Yi-Min Hsiao
Antonie van Leeuwenhoek.2022; 115(5): 589. CrossRef - Outer Membrane Vesicles: Biogenesis, Functions, and Issues
Rokas Juodeikis, Simon R. Carding
Microbiology and Molecular Biology Reviews.2022;[Epub] CrossRef - Recombinant Ax21 protein is a promising subunit vaccine candidate against Stenotrophomonas maltophilia in a murine infection model
Amal Tarek Sarhan, Mohammed Bahey-El-Din, Taha Ibrahim Zaghloul
Vaccine.2021; 39(32): 4471. CrossRef - Deciphering the functions of the outer membrane porin OprBXo involved in virulence, motility, exopolysaccharide production, biofilm formation and stress tolerance in Xanthomonas oryzae pv. oryzae
Nahee Bae, Hye‐Jee Park, Hanbi Park, Minyoung Kim, Sang‐Wook Han
Molecular Plant Pathology.2018; 19(12): 2527. CrossRef - The Ax21 protein influences virulence and biofilm formation in Stenotrophomonas maltophilia
Shi-qi An, Ji-liang Tang
Archives of Microbiology.2018; 200(1): 183. CrossRef - Lsp family proteins regulate antibiotic biosynthesis in Lysobacter enzymogenes OH11
Ruping Wang, Huiyong Xu, Yangyang Zhao, Juan Zhang, Gary Y Yuen, Guoliang Qian, Fengquan Liu
AMB Express.2017;[Epub] CrossRef - Systems and synthetic biology perspective of the versatile plant-pathogenic and polysaccharide-producing bacterium Xanthomonas campestris
Sarah Schatschneider, Jessica Schneider, Jochen Blom, Fabien Létisse, Karsten Niehaus, Alexander Goesmann, Frank-Jörg Vorhölter
Microbiology.2017; 163(8): 1117. CrossRef - Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria
Hye-Jee Park, Sang-Wook Han
The Plant Pathology Journal.2017; 33(6): 602. CrossRef - Deciphering the Role of Tyrosine Sulfation in Xanthomonas oryzae pv. oryzae Using Shotgun Proteomic Analysis
Hye-Jee Park, Chang-Jin Park, Nahee Bae, Sang-Wook Han
The Plant Pathology Journal.2016; 32(3): 266. CrossRef - The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia
Simon Devos, Laurence Van Oudenhove, Stephan Stremersch, Wouter Van Putte, Riet De Rycke, Gonzalez Van Driessche, Jolien Vitse, Koen Raemdonck, Bart Devreese
Frontiers in Microbiology.2015;[Epub] CrossRef
- VvpM, an Extracellular Metalloprotease of Vibrio vulnificus, Induces Apoptotic Death of Human Cells
-
Mi-Ae Lee , Jeong-A Kim , Yu Jin Yang , Mee-Young Shin , Soon-Jung Park , Kyu-Ho Lee
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J. Microbiol. 2014;52(12):1036-1043. Published online November 3, 2014
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DOI: https://doi.org/10.1007/s12275-014-4531-0
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48
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16
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Abstract
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A pathogenic bacterium, Vibrio vulnificus produces various
extracellular proteases including the elastolytic metalloprotease,
VvpE. In silico analysis of its genome revealed a VvpEhomologous
protease, VvpM whose proteolytic activity was
abolished by specific inhibitors against metalloproteases. To
investigate whether this newly identified protease has pathogenic
role in host interaction in addition to proteolytic role,
human cell lines were incubated with recombinant VvpM
(rVvpM). rVvpM-challenged cells showed typical morphological
changes found in cells under apoptosis. Apoptotic
cell death was further evidenced by estimating the Annexin
V-stained cells, whose proportions were dependent upon
the concentrations of rVvpM treated to human cells. To elucidate
the signaling pathway for VvpM-induced apoptosis,
three MAPKs were tested if their activation were mediated by
rVvpM. ERK1/2 was phosphorylated by treatment of rVvpM
and rVvpM-induced cell death was blocked by a specific inhibitor
against ERK1/2. In rVvpM-treated cells, the cytosolic
levels of cytochrome c were increased in a VvpM concentration-
dependent manner, while the levels of cytochrome c in
mitochondria were decreased. Cell deaths were accompanied
by apparent cleavages of procaspases-9 and -3 to the active
caspases-9 and -3, respectively. Therefore, this study demonstrates
that an extracellular metalloprotease of V. vulnificus,
VvpM induces apoptosis of human cells via a pathway consisting
of ERK activation, cytochrome c release, and then
activation of caspases-9 and -3.
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Citations
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- Pathology and pathogenesis of Vibrio infection in fish: A review
Tilusha Manchanayake, Annas Salleh, Mohammad Noor Azmai Amal, Ina Salwany Md Yasin, Mohd Zamri-Saad
Aquaculture Reports.2023; 28: 101459. CrossRef - Direct and indirect effects of pathogenic bacteria on the integrity of intestinal barrier
Lin-Zhen Shu, Yi-Dan Ding, Qing-Ming Xue, Wei Cai, Huan Deng
Therapeutic Advances in Gastroenterology.2023;[Epub] CrossRef - Vibrio vulnificus PlpA facilitates necrotic host cell death induced by the pore forming MARTX toxin
Changyi Cho, Sanghyeon Choi, Myung Hee Kim, Byoung Sik Kim
Journal of Microbiology.2022; 60(2): 224. CrossRef - The DNA binding domain of theVibrio vulnificusSmcR transcription factor is flexible and binds diverse DNA sequences
Jane D Newman, Meghan M Russell, Lixin Fan, Yun-Xing Wang, Giovanni Gonzalez-Gutierrez, Julia C van Kessel
Nucleic Acids Research.2021; 49(10): 5967. CrossRef - Melatonin restores Muc2 depletion induced by V. vulnificus VvpM via melatonin receptor 2 coupling with Gαq
Young-Min Lee, Jong Pil Park, Young Hyun Jung, Hyun Jik Lee, Jun Sung Kim, Gee Euhn Choi, Ho Jae Han, Sei-Jung Lee
Journal of Biomedical Science.2020;[Epub] CrossRef - The role of Vibrio vulnificus virulence factors and regulators in its infection-induced sepsis
Gang Li, Ming-Yi Wang
Folia Microbiologica.2020; 65(2): 265. CrossRef - Intestinal epithelial cell apoptosis due to a hemolytic toxin from Vibrio vulnificus and protection by a 36 kDa glycoprotein from Rhus verniciflua Stokes
Young-Min Lee, Jong Pil Park, Kye-Taek Lim, Sei-Jung Lee
Food and Chemical Toxicology.2019; 125: 46. CrossRef - The extracellular proteases produced by Vibrio parahaemolyticus
George Osei-Adjei, Xinxiang Huang, Yiquan Zhang
World Journal of Microbiology and Biotechnology.2018;[Epub] CrossRef - Repression of VvpM Protease Expression by Quorum Sensing and the cAMP-cAMP Receptor Protein Complex in Vibrio vulnificus
Jeong-A Kim, Mi-Ae Lee, You-Chul Jung, Bo-Ram Jang, Kyu-Ho Lee, Victor J. DiRita
Journal of Bacteriology.2018;[Epub] CrossRef - Classification and structural insight into vibriolysin-like proteases of Vibrio pathogenicity
JiaFeng Huang, BingQi Zeng, Dan Liu, RiBang Wu, Jiang Zhang, BinQiang Liao, HaiLun He, Fei Bian
Microbial Pathogenesis.2018; 117: 335. CrossRef - A Vibrio vulnificus VvpM Induces IL-1β Production Coupled with Necrotic Macrophage Death via Distinct Spatial Targeting by ANXA2
Sei-Jung Lee, Young Hyun Jung, Jun Sung Kim, Hyun Jik Lee, Sang Hun Lee, Kyu-Ho Lee, Kyung Ku Jang, Sang Ho Choi, Ho Jae Han
Frontiers in Cellular and Infection Microbiology.2017;[Epub] CrossRef - Vibrio vulnificus: An Environmental and Clinical Burden
Sing-Peng Heng, Vengadesh Letchumanan, Chuan-Yan Deng, Nurul-Syakima Ab Mutalib, Tahir M. Khan, Lay-Hong Chuah, Kok-Gan Chan, Bey-Hing Goh, Priyia Pusparajah, Learn-Han Lee
Frontiers in Microbiology.2017;[Epub] CrossRef - Crystal Structure of the Regulatory Domain of AphB from Vibrio vulnificus, a Virulence Gene Regulator
Nohra Park, Saemee Song, Garam Choi, Kyung Ku Jang, Inseong Jo, Sang Ho Choi, Nam-Chul Ha
Molecules and Cells.2017; 40(4): 299. CrossRef - The hydrogen peroxide hypersensitivity of OxyR2 in Vibrio vulnificus depends on conformational constraints
Inseong Jo, Dukyun Kim, Ye-Ji Bang, Jinsook Ahn, Sang Ho Choi, Nam-Chul Ha
Journal of Biological Chemistry.2017; 292(17): 7223. CrossRef - The Makes Caterpillars Floppy (MCF)-Like Domain of Vibrio vulnificus Induces Mitochondrion-Mediated Apoptosis
Shivangi Agarwal, Yeuming Zhu, David R. Gius, Karla J. F. Satchell, S. M. Payne
Infection and Immunity.2015; 83(11): 4392. CrossRef - Stationary‐phase induction of vvpS expression by three transcription factors: repression by LeuO and activation by SmcR and CRP
Jeong‐A. Kim, Jin Hwan Park, Mi‐Ae Lee, Hyun‐Jung Lee, Soon‐Jung Park, Kun‐Soo Kim, Sang‐Ho Choi, Kyu‐Ho Lee
Molecular Microbiology.2015; 97(2): 330. CrossRef
- Pseudomonas aeruginosa-induced IL-1β Production is Inhibited by Sophora flavescens via the NF-κB/inflammasome Pathways
-
Jung-Hoon Lee , Heesung Shin , Yong-Jae Kim , Se-Hwan Paek , Shouguang Jin , Un-Hwan Ha
-
J. Microbiol. 2014;52(12):1044-1049. Published online November 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4512-3
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46
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17
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Abstract
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The proinflammatory cytokine interleukin-1β plays an important role in protecting the host against airway infection; however, it can also trigger a massive influx of neutrophils into the airways, causing tissue damage. Anti-inflammatory treatments are particularly in demand for patients suffering from chronic inflammatory diseases. Sophora flavescens is a traditional herbal medicine used to reduce inflammation, but no study has examined its ability to block IL-1β production. Here, we show that S. flavescens reduced the Pseudomonas aeruginosa-induced expression of IL-1β by lung epithelial cells and macrophages. S. flavescens was also effective at reducing IL-1β production induced by either Staphylococcus aureus or phorbol 12-myristate 13-acetate, indicating that the effect is generalizable to diverse inflammatory stimuli. In addition, S. flavescens blocked the phosphorylation of IKKα/β, key upstream kinases involved in the degradation of IκBα, and the cleavage of caspase-1, a key component of the inflammasome. Thus, this study demonstrates that S. flavescens exerts its anti-inflammatory effects by blocking P. aeruginosa-mediated NF-κB/inflammasome activation and the subsequent production of IL-1β.
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Citations
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Current Microbiology.2023;[Epub] CrossRef - Chemical diversity, biological activities and Traditional uses of and important Chinese herb Sophora
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Zhao-hui Liu, Zhe Jin, Hong Zhao, Yao Lu, Hui Zhen, Ting Zou
Chinese Journal of Integrative Medicine.2021; 27(12): 883. CrossRef - Maackiain, a compound derived from Sophora flavescens, increases IL‐1β production by amplifying nigericin‐mediated inflammasome activation
Jin‐Won Huh, Jung‐Hoon Lee, Eekhyoung Jeon, Hyung Won Ryu, Sei‐Ryang Oh, Kyung‐Seop Ahn, Hyun Sik Jun, Un‐Hwan Ha
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Hong Li, Andrew Hung, Angela Wei Hong Yang
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Jung-Hoon Lee, Jisu Jeon, Fang Bai, Shouguang Jin, Weihui Wu, Un-Hwan Ha
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Xuping Wang, Rongzong Zheng, Xiaowen Huang, Zhujun Mao, Nani Wang, Hongyu Li, Chengping Wen, Dan Shou
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- Note] Oceanobacillus gochujangensis sp. nov., Isolated from gochujang a Traditional Korean Fermented Food
-
Seo-Jung Jang , Yu-Jin Kim , Sul-Hee Lee , Young-Seo Park , Jung-Min Park , Dong-Hoon Bai
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J. Microbiol. 2014;52(12):1050-1055. Published online July 30, 2014
-
DOI: https://doi.org/10.1007/s12275-014-4220-z
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43
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4
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Abstract
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A Gram-stain-positive, polar flagella-containing, rod-shaped,
obligate aerobic, endospore-forming bacterium, strain
TK1655T, was isolated from the traditional Korean food
gochujang. The 16S rRNA sequence of strain TK1655T was
a member of the genus Oceanobacillus similar to that of the
type strain of Oceanobacillus oncorhynchi subsp. incaldanensis
DSM 16557T (97.2%), O. oncorhynchi subsp. oncorhynchi JCM
12661T (97.1%), O. locisalsi KCTC 13253T (97.0%), and O.
sojae JCM 15792T (96.9%). Strain TK1655T was oxidase and
catalase positive. Colonies were circular, smooth, low convex,
cream in colour, and measured about 0.5–1.0 mm in diameter.
The range for growth was 20–40°C (optimal, 30°C), pH 6.0–
10.0 (optimal, 7.0), and 2–16% (w/v) NaCl (optimal, 2%).
Additionally, the cells contained meso-DAP, and the predominant
isoprenoid quinone was MK-7. The complex polar
lipids were consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol
(PG), phosphatidylcholine (PC). The major
cellular fatty acid components were iso-C15:0, anteiso-C15:0,
iso-C16:0, and anteiso-C17:0, and the DNA G+C content was
40.5%. DNA-DNA relatedness of our novel strain and reference
strain O. locisalsi KCTC 13253T, O. oncorhynchi subsp.
incaldanensis DSM 16557T, O. oncorhynchi subsp. oncorhynchi
JCM 12661T was 45.7, 43.8, and 41.9%. From the
results
of phenotypic, chemotaxonomic, and phylogenetic
analyses of strain TK1655T, we propose the novel species
Oceanobacillus gochujangensis sp. nov. The type strain is
TK1655T (=KCCM 101304T =KCTC 33014T =CIP 110582T
=NBRC 109637T).
-
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- Microbiota dynamics and volatile compounds in lupine based Moromi fermented at different salt concentrations
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- Erratum] Deodorization of Pig Slurry and Characterization of Bacterial Diversity Using 16S rDNA Sequence Analysis
-
Ok-Hwa Hwang , Sebastian Raveendar , Young-Ju Kim , Ji-Hun Kim , Tae-Hun Kim , Dong-Yoon Choi , Che Ok Jeon , Sung-Back Cho , Kyung-Tai Lee
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J. Microbiol. 2014;52(12):1056-1056.
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DOI: https://doi.org/10.1007/s12275-014-0704-0
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1
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Abstract
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In the article by Hwang et al. published in Journal of Microbiology 2014; 52, 918-919. Jung-Woo Choi was omitted as the 5th author of this paper. The authors should be
Ok-Hwa Hwang1†, Sebastian Raveendar1†, Young-Ju Kim1, Ji-Hun Kim1, Jung-Woo Choi1, Tae-Hun Kim1, Dong-Yoon Choi1, Che Ok Jeon2*, Sung-Back Cho1*, and Kyung-Tai Lee1*