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Volume 55(12); December 2017
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Review
Minireview] Electron microscopic observations of prokaryotic surface appendages
Ki Woo Kim
J. Microbiol. 2017;55(12):919-926.   Published online December 7, 2017
DOI: https://doi.org/10.1007/s12275-017-7369-4
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AbstractAbstract
Prokaryotic microbes possess a variety of appendages on their cell surfaces. The most commonly known surface appendages of bacteria include flagella, pili, curli, and spinae. Although archaea have archaella (archaeal flagella) and various types of pili that resemble those in bacteria, cannulae, and hami are unique to archaea. Typically involved in cell motility, flagella, the thickest appendages, are 20–26 nm and 10–14 nm wide in bacteria and archaea, respectively. Bacterial and archaeal pili are distinguished by their thin, short, hair-like structures. Curli appear as coiled and aggregative thin fibers, whereas spinae are tubular structures 50–70 nm in diameter in bacteria. Cannulae are characterized by ~25 nm-wide tubules that enter periplasmic spaces and connect neighboring archaeal cells. Hami are 1–3 μm in length and similar to barbed grappling hooks for attachment to bacteria. Recent advances in specimen preparation methods and image processing techniques have made cryo-transmission electron microscopy an essential tool for in situ structural analysis of microbes and their extracellular structures.

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Citations to this article as recorded by  
  • Mechanism of escape from the antibacterial activity of metal-based nanoparticles in clinically relevant bacteria: A systematic review
    Marco Felipe Salas-Orozco, Ana Cecilia Lorenzo-Leal, Idania de Alba Montero, Nuria Patiño Marín, Miguel Angel Casillas Santana, Horacio Bach
    Nanomedicine: Nanotechnology, Biology and Medicine.2024; 55: 102715.     CrossRef
  • Symbiotic Interactions of Archaea in Animal and Human Microbiomes
    Nancy Weiland-Bräuer
    Current Clinical Microbiology Reports.2023; 10(4): 161.     CrossRef
  • Jejubacter calystegiae gen. nov., sp. nov., moderately halophilic, a new member of the family Enterobacteriaceae, isolated from beach morning glory
    Lingmin Jiang, Dexin Wang, Jung-Sook Lee, Dae-Hyuk Kim, Jae Cheol Jeong, Cha Young Kim, Suk Weon Kim, Jiyoung Lee
    Journal of Microbiology.2020; 58(5): 357.     CrossRef
  • Role of Bacterial Cytoskeleton and Other Apparatuses in Cell Communication
    Divya Singhi, Preeti Srivastava
    Frontiers in Molecular Biosciences.2020;[Epub]     CrossRef
  • A versatile remote control system for functional expression of bacterial virulence genes based on the tetA promoter
    Marc Schulte, Torsten Sterzenbach, Katarzyna Miskiewicz, Laura Elpers, Michael Hensel, Nicole Hansmeier
    International Journal of Medical Microbiology.2019; 309(1): 54.     CrossRef
  • Paracoccus jeotgali sp. nov., isolated from Korean salted and fermented shrimp
    Juseok Kim, Joon Yong Kim, Hye Seon Song, In-Tae Cha, Seong Woon Roh, Se Hee Lee
    Journal of Microbiology.2019; 57(6): 444.     CrossRef
Journal Articles
Spirosoma migulaei sp. nov., isolated from soil
Joseph Okiria , Leonid N. Ten , Su-Jin Park , Seung-Yeol Lee , Dong Hoon Lee , In-Kyu Kang , Dae Sung Lee , Hee-Young Jung
J. Microbiol. 2017;55(12):927-932.   Published online December 7, 2017
DOI: https://doi.org/10.1007/s12275-017-7377-4
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AbstractAbstract
A Gram-stain-negative, non-motile, non-spore-forming, rodshaped, aerobic bacterium, designated 15J9-8T, was isolated from soil on Jeju Island, Republic of Korea. The isolate was able to grow between 10 and 30°C, pH 6.5–8.5, and in presence of 0–1% (w/v) NaCl. The results of comparative 16S rRNA gene sequence analysis indicated that strain 15J9-8T represented a member of the family Cytophagaceae, phylum Bacteroidetes, and was most closely related to Spirosoma aerophilum 5516J-17T (96.1% similarity), Spirosoma pulveris JSH5-14T (95.6%), and Spirosoma linguale DSM 74T (95.2%). The G + C content of the genomic DNA of the isolate was 47.0 mol%. Strain 15J9-8T contained summed feature 3 (C16:1 ω7c/C16:1 ω6c), C16:1 ω5c, and iso-C15:0 as the major fatty acids, phosphatidylethanolamine and an unidentified aminophospholipid as the main polar lipids, and menaquinone MK-7 as the predominant respiratory quinone. On the basis of its phenotypic and genotypic properties, and phylogenetic distinctiveness, strain 15J9-8T should be classified as a representative of a novel species of the genus Spirosoma, for which the name Spirosoma migulaei sp. nov. is proposed. The type strain is 15J9-8T (=KCTC 52028T =JCM 31996T).

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  • Isolation of Spirosoma foliorum sp. nov. from the fallen leaf of Acer palmatum by a novel cultivation technique
    Ho Le Han, Dian Alfian Nurcahyanto, Neak Muhammad, Yong-Jae Lee, Tra T. H. Nguyen, Song-Gun Kim, Sook Sin Chan, Kuan Shiong Khoo, Kit Wayne Chew, Pau Loke Show, Thi Ngoc Thu Tran, Thi Dong Phuong Nguyen, Chen Yaw Chiu
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  • Spirosoma rhododendri sp. nov., isolated from a flower of royal azalea (Rhododendron schlippenbachii)
    Miyoung Won, Seung-Beom Hong, Byeong-Hak Han, Soon-Wo Kwon
    International Journal of Systematic and Evolutionary Microbiology .2022;[Epub]     CrossRef
  • Fibrivirga algicola gen. nov., sp. nov., an algicidal bacterium isolated from a freshwater river
    Sanghwa Park, Ja Young Cho, Dong-Hyun Jung, Seok Won Jang, Jung Hye Eom, Seung Won Nam, Dae Ryul Kwon, Jaewon Ryu, Keug Tae Kim
    Antonie van Leeuwenhoek.2022; 115(7): 899.     CrossRef
  • Spirosoma telluris sp. nov. and Spirosoma arboris sp. nov. isolated from soil and tree bark, respectively
    Heeyoung Kang, Inseong Cha, Haneul Kim, Kiseong Joh
    International Journal of Systematic and Evolutionary Microbiology .2020; 70(10): 5355.     CrossRef
  • Complete Genome Sequence of Spirosoma sp. Strain KCTC 42546, Isolated from a Reservoir in South Korea
    Pokchut Kusolkumbot, Song-Gun Kim, Chatrudee Suwannachart, Kenneth M. Stedman
    Microbiology Resource Announcements.2020;[Epub]     CrossRef
  • Spirosoma sordidisoli sp. nov., a propanil-degrading bacterium isolated from a herbicide-contaminated soil
    Long Zhang, Xi-Yi Zhou, Xiao-Jing Su, Qiang Hu, Jian-Dong Jiang
    Antonie van Leeuwenhoek.2019; 112(10): 1523.     CrossRef
  • Spirosoma utsteinense sp. nov. isolated from Antarctic ice-free soils from the Utsteinen region, East Antarctica
    Guillaume Tahon, Liesbeth Lebbe, Anne Willems
    International Journal of Systematic and Evolutionary Microbiology .2019;[Epub]     CrossRef
  • List of new names and new combinations previously effectively, but not validly, published
    Aharon Oren, George Garrity
    International Journal of Systematic and Evolutionary Microbiology.2018; 68(5): 1411.     CrossRef
Virgibacillus kimchii sp. nov., a halophilic bacterium isolated from kimchi
Young Joon Oh , Ja-Young Jang , Seul Ki Lim , Min-Sung Kwon , Jieun Lee , NamHee Kim , Mi-Young Shin , Hyo Kyeong Park , Myung-Ji Seo , Hak-Jong Choi
J. Microbiol. 2017;55(12):933-938.   Published online December 7, 2017
DOI: https://doi.org/10.1007/s12275-017-7386-3
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AbstractAbstract
A Gram-stain-positive, halophilic, rod-shaped, non-motile, spore forming bacterium, strain NKC1-2T, was isolated from kimchi, a Korean fermented food. Comparative analysis based on 16S rRNA gene sequence demonstrated that the isolated strain was a species of the genus Virgibacillus. Strain NKC1- 2T exhibited high level of 16S rRNA gene sequence similarity with the type strains of Virgibacillus xinjiangensis SL6-1T (96.9%), V. sediminis YIM kkny3T (96.8%), and V. salarius SA-Vb1T (96.7%). The isolate grew at pH 6.5–10.0 (optimum, pH 8.5–9.0), 0.0–25.0% (w/v) NaCl (optimum, 10–15% NaCl), and 15–50°C (optimum, 37°C). The major menaquinone in the strain was menaquinone-7, and the main peptidoglycan of the strain was meso-diaminopimelic acid. The predominant fatty acids of the strain were iso-C14:0, anteisio-C15:0, iso- C15:0, and iso-C16:0 (other components were < 10.0%). The polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The genomic DNA G + C content of NKC1-2T was 42.5 mol%. On the basis of these findings, strain NKC1- 2T is proposed as a novel species in the genus Virgibacillus, for which the name Virgibacillus kimchii sp. nov. is proposed (=KACC 19404T =JCM 32284T). The type strain of Virgibacillus kimchii is NKC1-2T.

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  • Bioprospecting for moderately halophilic eubacteria for potential biotechnological applications from Sambhar Lake, Rajasthan, India
    Saloni Singh, Ayushi Goyal, Kakoli Dutt
    The Applied Biology & Chemistry Journal.2024; : 12.     CrossRef
  • Salicibibacter cibarius sp. nov. and Salicibibacter cibi sp. nov., two novel species of the family Bacillaceae isolated from kimchi
    Young Joon Oh, Joon Yong Kim, Seul Ki Lim, Min-Sung Kwon, Hak-Jong Choi
    Journal of Microbiology.2021; 59(5): 460.     CrossRef
  • Salicibibacter halophilus sp. nov., a moderately halophilic bacterium isolated from kimchi
    Young Joon Oh, Joon Yong Kim, Hyo Kyeong Park, Ja-Young Jang, Seul Ki Lim, Min-Sung Kwon, Hak-Jong Choi
    Journal of Microbiology.2019; 57(11): 997.     CrossRef
  • Mining biosynthetic gene clusters in Virgibacillus genomes
    Ghofran Othoum, Salim Bougouffa, Ameerah Bokhari, Feras F. Lafi, Takashi Gojobori, Heribert Hirt, Ivan Mijakovic, Vladimir B. Bajic, Magbubah Essack
    BMC Genomics.2019;[Epub]     CrossRef
  • List of new names and new combinations that have appeared in effective publications outside of the IJSEM and are submitted for valid publication
    Aharon Oren, George M. Garrity
    International Journal of Systematic and Evolutionary Microbiology .2019;[Epub]     CrossRef
  • Community structures and genomic features of undesirable white colony-forming yeasts on fermented vegetables
    Joon Yong Kim, Juseok Kim, In-Tae Cha, Min Young Jung, Hye Seon Song, Yeon Bee Kim, Changsu Lee, Seung-Yeon Kang, Jin-Woo Bae, Yoon-E Choi, Tae-Woon Kim, Seong Woon Roh
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  • Salicibibacter kimchii gen. nov., sp. nov., a moderately halophilic and alkalitolerant bacterium in the family Bacillaceae, isolated from kimchi
    Ja-Young Jang, Young Joon Oh, Seul Ki Lim, Hyo Kyeong Park, Changsu Lee, Joon Yong Kim, Mi-Ai Lee, Hak-Jong Choi
    Journal of Microbiology.2018; 56(12): 880.     CrossRef
  • Lactobacillus curvatusHY7601 andLactobacillus plantarumKY1032 Cell Extracts Inhibit Adipogenesis in 3T3-L1 and HepG2 Cells
    Woon Hee Jeung, Jae-Jung Shim, Seon-Wook Woo, Jae-Hun Sim, Jung-Lyoul Lee
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Cecal microbiome divergence of broiler chickens by sex and body weight
Kyu-Chan Lee , Dong Yong Kil , Woo Jun Sul
J. Microbiol. 2017;55(12):939-945.   Published online December 7, 2017
DOI: https://doi.org/10.1007/s12275-017-7202-0
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AbstractAbstract
The divergence of gut bacterial community on broiler chickens has been reported as potentially possible keys to enhancing nutrient absorption, immune systems, and increasing poultry health and performance. Thus, we compared cecal bacterial communities and functional predictions by sex and body weight regarding the association between cecal microbiota and chicken growth performance. In this study, a total of 12 male and 12 female 1-day-old broiler chickens were raised for 35 days in 2 separate cages. Chickens were divided into 3 subgroups depending on body weight (low, medium, and high) by each sex. We compared chicken cecal microbiota compositions and its predictive functions by sex and body weight difference. We found that bacterial 16S rRNA genes were classified as 3 major phyla (Bacteroidetes, Firmicutes, and Proteobacteria), accounting for > 98% of the total bacterial community. The profiling of different bacterial taxa and predictive metagenome functions derived from 16S rRNA genes were performed over chicken sex and bodyweight. Male chickens were related to the enrichment of Bacteroides while female chickens were to the enrichment of Clostridium and Shigella. Male chickens with high body weight were associated with the enrichment of Faecalibacterium and Shuttleworthia. Carbohydrate and lipid metabolisms were suggested as candidate functions for weight gain in the males. This suggests that the variation of cecal bacterial communities and their functions by sex and body weight may be associated with the differences in the growth potentials of broiler chickens.

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Effects of dietary poly-β-hydroxybutyrate (PHB) on microbiota composition and the mTOR signaling pathway in the intestines of Litopenaeus vannamei
Yafei Duan , Yue Zhang , Hongbiao Dong , Yun Wang , Jiasong Zhang
J. Microbiol. 2017;55(12):946-954.   Published online December 7, 2017
DOI: https://doi.org/10.1007/s12275-017-7273-y
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AbstractAbstract
Poly-β-hydroxybutyrate (PHB) is a natural polymer of the short chain fatty acid β-hydroxybutyrate, which acts as a microbial control agent. The mammalian target of the rapamycin (mTOR) signaling pathway plays a crucial role in intestine inflammation and epithelial morphogenesis. In this study, we examined the composition of intestine microbiota, and mTOR signaling-related gene expression in Pacific white shrimp Litopenaeus vannamei fed diets containing different levels of PHB: 0% (Control), 1% (PHB1), 3% (PHB3), and 5% (PHB5) (w/w) for 35 days. High-throughput sequencing analysis revealed that dietary PHB altered the composition and diversity of intestine microbiota, and that the microbiota diversity decreased with the increasing doses of PHB. Specifically, dietary PHB increased the relative abundance of Proteobacteria and Tenericutes in the PHB1 and PHB5 groups, respectively, and increased that of Gammaproteobacteria in the three PHB groups. Alternatively, PHB decreased Alphaproteobacteria in the PHB3 and PHB5 groups. At the genus level, dietary PHB increased the abundance of beneficial bacteria, such as Bacillus, Lactobacillus, Lactococcus, Clostridium, and Bdellovibrio. The relative mRNA expression levels of the mTOR signaling-related genes TOR, 4E-BP, eIF4E1α, and eIF4E2 all increased in the three PHB treatment groups. These
results
revealed that dietary PHB supplementation had a beneficial effect on intestine health of L. vannamei by modulating the composition of intestine microbiota and activating mTOR signaling.

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Metabolism-mediated induction of zinc tolerance in Brassica rapa by Burkholderia cepacia CS2-1
Sang-Mo Kang , Raheem Shahzad , Saqib Bilal , Abdul Latif Khan , Young-Hyun You , Won-Hee Lee , Hee-La Ryu , Ko-Eun Lee , In-Jung Lee
J. Microbiol. 2017;55(12):955-965.   Published online December 7, 2017
DOI: https://doi.org/10.1007/s12275-017-7305-7
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AbstractAbstract
Brassica rapa (Chinese cabbage) is an essential component of traditional Korean food. However, the crop is often subject to zinc (Zn+) toxicity from contaminated irrigation water, which, as a result, compromises plant growth and production, as well as the health of human consumers. The present study investigated the bioaccumulation of Zn+ by Burkholderia cepacia CS2-1 and its effect on the heavy metal tolerance of Chinese cabbage. Strain CS2-1 was identified and characterized on the basis of 16S rRNA sequences and phylogenetic analysis. The strain actively produced indole-3-acetic acid (3.08 ± 0.21 μg/ml) and was also able to produce siderophore, solubilize minerals, and tolerate various concentrations of Zn+. The heavy metal tolerance of B. rapa plants was enhanced by CS2-1 inoculation, as indicated by growth attributes, Zn+ uptake, amino acid synthesis, antioxidant levels, and endogenous hormone (ABA and SA) synthesis. Without inoculation, the application of Zn+ negatively affected the growth and physiology of B. rapa plants. However, CS2-1 inoculation improved plant growth, lowered Zn+ uptake, altered both amino acid regulation and levels of flavonoids and phenolics, and significantly decreased levels of superoxide dismutase, endogenous abscisic acid, and salicylic acid. These findings indicate that B. cepacia CS2-1 is suitable for bioremediation against Zn+-induced oxidative stress.

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  • Phytohormones enabled endophytic Penicillium funiculosum LHL06 protects Glycine max L. from synergistic toxicity of heavy metals by hormonal and stress-responsive proteins modulation
    Saqib Bilal, Raheem Shahzad, Abdul Latif Khan, Ahmed Al-Harrasi, Chang Kil Kim, In-Jung Lee
    Journal of Hazardous Materials.2019; 379: 120824.     CrossRef
  • Amelioration of heavy metal stress by endophytic Bacillus amyloliquefaciens RWL-1 in rice by regulating metabolic changes: potential for bacterial bioremediation
    Raheem Shahzad, Saqib Bilal, Muhammad Imran, Abdul Latif Khan, Areej Ahmed Alosaimi, Hussah Abdullah Al-Shwyeh, Hanan Almahasheer, Suriya Rehman, In-Jung Lee
    Biochemical Journal.2019; 476(21): 3385.     CrossRef
Direct interaction between the transcription factors CadC and OmpR involved in the acid stress response of Salmonella enterica
Yong Heon Lee , Ji Hye Kim
J. Microbiol. 2017;55(12):966-972.   Published online December 7, 2017
DOI: https://doi.org/10.1007/s12275-017-7410-7
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AbstractAbstract
In Salmonella enterica serovar Typhimurium, the acid-sensing regulator CadC activates transcription of the cadBA operon which contributes to the acid tolerance response. The DNA-binding response regulator OmpR in two-component regulatory system with EnvZ binds to its own promoter for autoinduction. We previously reported that CadC exerts a negative influence on ompR transcription during acid adaptation. However, its underlying mechanisms remain to be elucidated. Here we show that the level of OmpR protein is gradually reduced by a gradual increase in the CadC level using an arabinose-inducible expression system, indicating there exists a negative correlation between the expression levels of two transcription factors. To explore the molecular basis for OmpR repression by CadC, we performed in vitro binding assays and determined that CadC directly interacts with OmpR. We further show that inactivation of cadC inhibits transcription of the fliC gene, which encodes the major flagellar subunit,
result
ing in impaired flagellar motility under acid-adaptation conditions. Together, our findings suggest that CadC may repress autoinduction of the OmpR response regulator through their direct interaction.

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  • Isolation and characterization of resistant variants of Salmonella Typhimurium after sequential exposure to plasma activated water (PAW)
    Elisa Pagán, Foteini Pavli, Sarah Happiette, Daniel Berdejo, Ruben Gatt, Rafael Pagán, Vasilis Valdramidis, Diego García-Gonzalo
    Innovative Food Science & Emerging Technologies.2024; 93: 103633.     CrossRef
  • A current insight into Salmonella’s inducible acid resistance
    Alkmini Gavriil, Ilias Giannenas, Panagiotis N. Skandamis
    Critical Reviews in Food Science and Nutrition.2024; : 1.     CrossRef
  • Molecular mechanism of proteolytic cleavage-dependent activation of CadC-mediated response to acid in E. coli
    Min Chen, Ye Shang, Wenhao Cui, Xiaomeng Wang, Jiakun Zhu, Hongjie Dong, Hongwei Wang, Tiantian Su, Weiwei Wang, Kundi Zhang, Bingqing Li, Sujuan Xu, Wei Hu, Fengyu Zhang, Lichuan Gu
    Communications Biology.2024;[Epub]     CrossRef
  • Transmembrane Transcription Regulators Are Widespread in Bacteria and Archaea
    Lucas M. Demey, Vadim M. Gumerov, Jiawei Xing, Igor B. Zhulin, Victor J. DiRita, Beile Gao
    Microbiology Spectrum.2023;[Epub]     CrossRef
  • Host acid signal controls Salmonella flagella biogenesis through CadC-YdiV axis
    Weiwei Wang, Yingying Yue, Min Zhang, Nannan Song, Haihong Jia, Yuanji Dai, Fengyu Zhang, Cuiling Li, Bingqing Li
    Gut Microbes.2022;[Epub]     CrossRef
  • Recent advances in understanding the effect of acid-adaptation on the cross-protection to food-related stress of common foodborne pathogens
    Ricardo A. Wu, Hyun-Gyun Yuk, Donghong Liu, Tian Ding
    Critical Reviews in Food Science and Nutrition.2022; 62(26): 7336.     CrossRef
  • Metabolic adaptability shifts of cell membrane fatty acids of Komagataeibacter hansenii HDM1-3 improve acid stress resistance and survival in acidic environments
    Yuanjing Li, Pengfei Yan, Qingyun Lei, Bingyu Li, Yue Sun, Shuangfei Li, Hong Lei, Ning Xie
    Journal of Industrial Microbiology and Biotechnology.2019; 46(11): 1491.     CrossRef
Guinea pig complement potently measures vibriocidal activity of human antibodies in response to cholera vaccines
Kyoung Whun Kim , Soyoung Jeong , Ki Bum Ahn , Jae Seung Yang , Cheol-Heui Yun , Seung Hyun Han
J. Microbiol. 2017;55(12):973-978.   Published online December 7, 2017
DOI: https://doi.org/10.1007/s12275-017-7478-0
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AbstractAbstract
The vibriocidal assay using guinea pig complement is widely used for the evaluation of immune responses to cholera vaccines in human clinical trials. However, it is unclear why guinea pig complement has been used over human complement in the measurement of vibriocidal activity of human sera and there have not been comparison studies for the use of guinea pig complement over those from other species. Therefore, we comparatively investigated the effects of complements derived from human, guinea pig, rabbit, and sheep on vibriocidal activity. Complements from guinea pig, rabbit, and human showed concentration-dependent vibriocidal activity in the presence of quality control serum antibodies. Of these complements, guinea pig complement was the most sensitive and effective over a wide concentration range. When the vibriocidal activity of complements was measured in the absence of serum antibodies, human, sheep, and guinea pig complements showed vibriocidal activity up to 40-fold, 20- fold, and 1-fold dilution, respectively. For human pre- and post-vaccination sera, the most potent vibriocidal activity was observed when guinea pig complement was used. In addition, the highest fold-increases between pre- and post- vaccinated sera were obtained with guinea pig complement. Furthermore, human complement contained a higher amount of V. cholerae- and its lipopolysaccharide-specific antibodies than guinea pig complement. Collectively, these results suggest that guinea pig complements are suitable for vibriocidal assays due to their high sensitivity and effectiveness to human sera.

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  • Immunogenicity and protective efficacy of a live, oral cholera vaccine formulation stored outside-the-cold-chain for 140 days
    Tew Hui Xian, Kurunathan Sinniah, Chan Yean Yean, Venkateskumar Krishnamoorthy, Mohd Baidi Bahari, Manickam Ravichandran, Guruswamy Prabhakaran
    BMC Immunology.2020;[Epub]     CrossRef
  • A high-throughput, bead-based, antigen-specific assay to assess the ability of antibodies to induce complement activation
    Stephanie Fischinger, Jonathan K. Fallon, Ashlin R. Michell, Thomas Broge, Todd J. Suscovich, Hendrik Streeck, Galit Alter
    Journal of Immunological Methods.2019; 473: 112630.     CrossRef
  • Characterization of antibody response in patients with acute and chronic chikungunya virus disease
    Fatih Anfasa, Stephanie M. Lim, Susan Fekken, Robert Wever, Albert D.M.E. Osterhaus, Byron E.E. Martina
    Journal of Clinical Virology.2019; 117: 68.     CrossRef
Comparison of anti-influenza virus activity and pharmacokinetics of oseltamivir free base and oseltamivir phosphate
Jin Soo Shin , Keun Bon Ku , Yejin Jang , Yi-Seul Yoon , Daeho Shin , Oh Seung Kwon , Yun Young Go , Seong Soon Kim , Myoung Ae Bae , Meehyein Kim
J. Microbiol. 2017;55(12):979-983.   Published online December 7, 2017
DOI: https://doi.org/10.1007/s12275-017-7371-x
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AbstractAbstract
Influenza viruses are major human respiratory pathogens that cause high morbidity and mortality worldwide. Currently, prophylactic vaccines and therapeutic antiviral agents are used to prevent and control influenza virus infection. Oseltamivir free base (OSV-FB), a modified generic antiviral drug of Tamiflu (oseltamivir phosphate, OSV-P), was launched in the Republic of Korea last year. Here, we examine the bioequivalence of these two compounds by assessing their antiviral efficacy in infected cells and in a mouse model. It was observed that both antivirals showed comparable efficacy against 11 different influenza A and B viruses in vitro. Moreover, in mice infected with influenza A virus (mouse-adapted A/Puerto Rico/8/34), they showed a dose-dependent therapeutic activity and alleviated infection-mediated reductions in body weight, leading to significantly better survival. There was histopathological disappearance of virus-induced inflammatory cell infiltration of the lung after oral treatment with either antiviral agent (at 10 mg/kg). Pharmacokinetic analysis also exhibited similar plasma concentrations of the active drug, oseltamivir carboxylate, metabolised from both OSVB and OSV-P. This is the first report showing bioequivalence of OSV-FB to its phosphate salt form in the mouse system. The free base drug has some beneficial points including simple drug formulation process and reduced risk of undesirable cation-phosphate precipitation within solution. The long term stability of OSV-FB requires further monitoring when it is provided as a national stock in readiness for an influenza pandemic.

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    Jing Yu, Jian-Ming Liu, Hui-Yi Chen, Wei-Ming Xiong
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  • A Lysosome-Targeting hNEU1 Inhibitor Treats Myocardial Infarction: A Potential Therapeutic Breakthrough
    Wen Zhou, Wanxiang Yang, Ping Jiang, Shaohua Gou
    Journal of Medicinal Chemistry.2024; 67(18): 16899.     CrossRef
  • The possible techniques that used to improve the bioavailablity, pharmacological activity, solubility and permeability of anti-viral drugs: Insight for COVID-19 antiviral drugs
    Ghassan Mudher Hashim , Ghaidaa S. Hameed , Dalya Basil Hanna
    Al Mustansiriyah Journal of Pharmaceutical Sciences.2023; 23(3): 231.     CrossRef
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    Ran Chen, Tingting Wang, Jie Song, Daojun Pu, Dan He, Jianjun Li, Jie Yang, Kailing Li, Cailing Zhong, Jingqing Zhang
    International Journal of Nanomedicine.2021; Volume 16: 4959.     CrossRef
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    Helin Li, Xiaoyu Wu, Xin Li, Xiaobing Cao, Yanjun Li, Huaru Cao, Yongzhi Men
    Frontiers in Chemistry.2021;[Epub]     CrossRef
  • Development of Sustained Release Oseltamivir Phosphate Dry Powder Inhaler: In-Vitro Characterization and In-Vivo Toxicological Studies
    Harshal Sahastrabudhe, Prathmesh Kenjale, Varsha Pokharkar
    Current Drug Delivery.2020; 17(8): 703.     CrossRef
  • In Vitro and In Vivo Antiviral Activity of Nylidrin by Targeting the Hemagglutinin 2-Mediated Membrane Fusion of Influenza A Virus
    Yejin Jang, Jin Soo Shin, Joo-Youn Lee, Heegwon Shin, Sang Jick Kim, Meehyein Kim
    Viruses.2020; 12(5): 581.     CrossRef
  • A new naphthoquinone analogue and antiviral constituents from the root of Rhinacanthus nasutus
    Tran Minh Ngoc, Nguyen Thi Thanh Phuong, Nguyen Minh Khoi, SeonJu Park, Hee Jae Kwak, Nguyen Xuan Nhiem, Bui Thi Thu Trang, Bui Huu Tai, Jae-Hyoung Song, Hyun-Jeong Ko, Seung Hyun Kim
    Natural Product Research.2019; 33(3): 360.     CrossRef
  • Salinomycin Inhibits Influenza Virus Infection by Disrupting Endosomal Acidification and Viral Matrix Protein 2 Function
    Yejin Jang, Jin Soo Shin, Yi-Seul Yoon, Yun Young Go, Hye Won Lee, Oh Seung Kwon, Sehee Park, Man-Seong Park, Meehyein Kim, Jae U. Jung
    Journal of Virology.2018;[Epub]     CrossRef
  • The Cranberry Extract Oximacro® Exerts in vitro Virucidal Activity Against Influenza Virus by Interfering With Hemagglutinin
    Anna Luganini, Maria E. Terlizzi, Gianluca Catucci, Gianfranco Gilardi, Massimo E. Maffei, Giorgio Gribaudo
    Frontiers in Microbiology.2018;[Epub]     CrossRef
An ethanol extract of Lysimachia mauritiana exhibits inhibitory activity against hepatitis E virus genotype 3 replication
Seong Eun Jin , Jung-Eun Kim , Sun Yeou Kim , Bang Ju Park , Yoon-Jae Song
J. Microbiol. 2017;55(12):984-988.   Published online December 7, 2017
DOI: https://doi.org/10.1007/s12275-017-7477-1
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AbstractAbstract
Hepatitis E virus (HEV) is an etiological agent of acute hepatitis E, a self-limiting disease prevalent in developing countries. HEV can cause fulminant hepatic failure with high mortality rates in pregnant women, and genotype 3 is reported to trigger chronic hepatitis in immunocompromised individuals worldwide. Screening of plant extracts for compounds with potential anti-HEV effects led to the identification of a 70% ethanol extract of Lysimachia mauritiana (LME) that interferes with replication of the swine HEV genotype 3 replicon. Furthermore, LME significantly inhibited replication of HEV genotype 3 and expression of HEV ORF2 in infected cells without exerting cytotoxic effects. Collectively, our findings demonstrate the potential utility of LME in the development of novel antiviral drugs against HEV infection.

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    Flower Research Journal.2024; 32(2): 103.     CrossRef
  • Lysimachia mauritiana Lam. Extract Alleviates Airway Inflammation Induced by Particulate Matter Plus Diesel Exhaust Particles in Mice
    Yoon-Young Sung, Seung-Hyung Kim, Won-Kyung Yang, Heung Joo Yuk, Mi-Sun Kim, Dong-Seon Kim
    Nutrients.2024; 16(21): 3732.     CrossRef
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    Liangyu Hu, Chunying Wang, Yang Zhang
    European Journal of Integrative Medicine.2024; 72: 102419.     CrossRef
  • HPLC Analysis of Polyphenolic Compounds in Lysimachia nummularia L. and Comparative Determination of Antioxidant Capacity
    Felicia Suciu, Iuliana Stoicescu, Elena Lupu, Adina Musuc, Antoanela Popescu, Magdalena Mititelu, Adrian Roșca, Denisa-Elena Dumitrescu, Florin Badea, Aureliana Caraiane, Victoria Badea
    Applied Sciences.2023; 13(4): 2159.     CrossRef
  • Hepatitis E Virus Infection: Circulation, Molecular Epidemiology, and Impact on Global Health
    Srinivas Reddy Pallerla, Dominik Harms, Reimar Johne, Daniel Todt, Eike Steinmann, Mathias Schemmerer, Jürgen J. Wenzel, Jörg Hofmann, James Wai Kuo Shih, Heiner Wedemeyer, C.-Thomas Bock, Thirumalaisamy P. Velavan
    Pathogens.2020; 9(10): 856.     CrossRef
  • Hepatitis E Virus Drug Development
    Volker Kinast, Thomas L Burkard, Daniel Todt, Eike Steinmann
    Viruses.2019; 11(6): 485.     CrossRef
Published Erratum
Corrigendum] Application of high-resolution melting analysis for differentiation of spoilage yeasts
Mine Erdem , Zülal Kesmen , Esra Özbekar , Bülent Çetin , Hasan Yetim
J. Microbiol. 2017;55(12):989-989.
DOI: https://doi.org/10.1007/s12275-017-0671-3
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AbstractAbstract
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