Prokaryotic microbes possess a variety of appendages on their
cell surfaces. The most commonly known surface appendages
of bacteria include flagella, pili, curli, and spinae. Although
archaea have archaella (archaeal flagella) and various types
of pili that resemble those in bacteria, cannulae, and hami
are unique to archaea. Typically involved in cell motility, flagella,
the thickest appendages, are 20–26 nm and 10–14 nm
wide in bacteria and archaea, respectively. Bacterial and archaeal
pili are distinguished by their thin, short, hair-like
structures. Curli appear as coiled and aggregative thin fibers,
whereas spinae are tubular structures 50–70 nm in diameter
in bacteria. Cannulae are characterized by ~25 nm-wide tubules
that enter periplasmic spaces and connect neighboring
archaeal cells. Hami are 1–3 μm in length and similar to
barbed grappling hooks for attachment to bacteria. Recent
advances in specimen preparation methods and image processing
techniques have made cryo-transmission electron
microscopy an essential tool for in situ structural analysis of
microbes and their extracellular structures.
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A Gram-stain-negative, non-motile, non-spore-forming, rodshaped,
aerobic bacterium, designated 15J9-8T, was isolated
from soil on Jeju Island, Republic of Korea. The isolate was
able to grow between 10 and 30°C, pH 6.5–8.5, and in presence
of 0–1% (w/v) NaCl. The results of comparative 16S
rRNA gene sequence analysis indicated that strain 15J9-8T
represented a member of the family Cytophagaceae, phylum
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JSH5-14T (95.6%), and Spirosoma linguale DSM 74T (95.2%).
The G + C content of the genomic DNA of the isolate was
47.0 mol%. Strain 15J9-8T contained summed feature 3 (C16:1
ω7c/C16:1 ω6c), C16:1 ω5c, and iso-C15:0 as the major fatty acids,
phosphatidylethanolamine and an unidentified aminophospholipid
as the main polar lipids, and menaquinone MK-7
as the predominant respiratory quinone. On the basis of its
phenotypic and genotypic properties, and phylogenetic distinctiveness,
strain 15J9-8T should be classified as a representative
of a novel species of the genus Spirosoma, for which the
name Spirosoma migulaei sp. nov. is proposed. The type strain
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A Gram-stain-positive, halophilic, rod-shaped, non-motile,
spore forming bacterium, strain NKC1-2T, was isolated from
kimchi, a Korean fermented food. Comparative analysis based
on 16S rRNA gene sequence demonstrated that the isolated
strain was a species of the genus Virgibacillus. Strain NKC1-
2T exhibited high level of 16S rRNA gene sequence similarity
with the type strains of Virgibacillus xinjiangensis SL6-1T
(96.9%), V. sediminis YIM kkny3T (96.8%), and V. salarius
SA-Vb1T (96.7%). The isolate grew at pH 6.5–10.0 (optimum,
pH 8.5–9.0), 0.0–25.0% (w/v) NaCl (optimum, 10–15% NaCl),
and 15–50°C (optimum, 37°C). The major menaquinone in
the strain was menaquinone-7, and the main peptidoglycan
of the strain was meso-diaminopimelic acid. The predominant
fatty acids of the strain were iso-C14:0, anteisio-C15:0, iso-
C15:0, and iso-C16:0 (other components were < 10.0%). The
polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol.
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was 42.5 mol%. On the basis of these findings, strain NKC1-
2T is proposed as a novel species in the genus Virgibacillus,
for which the name Virgibacillus kimchii sp. nov. is proposed
(=KACC 19404T =JCM 32284T). The type strain of Virgibacillus
kimchii is NKC1-2T.
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has been reported as potentially possible keys to enhancing
nutrient absorption, immune systems, and increasing
poultry health and performance. Thus, we compared cecal
bacterial communities and functional predictions by sex and
body weight regarding the association between cecal microbiota
and chicken growth performance. In this study, a total
of 12 male and 12 female 1-day-old broiler chickens were
raised for 35 days in 2 separate cages. Chickens were divided
into 3 subgroups depending on body weight (low, medium,
and high) by each sex. We compared chicken cecal microbiota
compositions and its predictive functions by sex and body
weight difference. We found that bacterial 16S rRNA genes
were classified as 3 major phyla (Bacteroidetes, Firmicutes,
and Proteobacteria), accounting for > 98% of the total bacterial
community. The profiling of different bacterial taxa and
predictive metagenome functions derived from 16S rRNA
genes were performed over chicken sex and bodyweight. Male
chickens were related to the enrichment of Bacteroides while
female chickens were to the enrichment of Clostridium and
Shigella. Male chickens with high body weight were associated
with the enrichment of Faecalibacterium and Shuttleworthia.
Carbohydrate and lipid metabolisms were suggested as candidate
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functions by sex and body weight may be associated with the
differences in the growth potentials of broiler chickens.
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Poly-β-hydroxybutyrate (PHB) is a natural polymer of the
short chain fatty acid β-hydroxybutyrate, which acts as a
microbial control agent. The mammalian target of the rapamycin
(mTOR) signaling pathway plays a crucial role in intestine
inflammation and epithelial morphogenesis. In this
study, we examined the composition of intestine microbiota,
and mTOR signaling-related gene expression in Pacific white
shrimp Litopenaeus vannamei fed diets containing different
levels of PHB: 0% (Control), 1% (PHB1), 3% (PHB3), and 5%
(PHB5) (w/w) for 35 days. High-throughput sequencing analysis
revealed that dietary PHB altered the composition and
diversity of intestine microbiota, and that the microbiota diversity
decreased with the increasing doses of PHB. Specifically,
dietary PHB increased the relative abundance of Proteobacteria
and Tenericutes in the PHB1 and PHB5 groups,
respectively, and increased that of Gammaproteobacteria in
the three PHB groups. Alternatively, PHB decreased Alphaproteobacteria
in the PHB3 and PHB5 groups. At the genus
level, dietary PHB increased the abundance of beneficial bacteria,
such as Bacillus, Lactobacillus, Lactococcus, Clostridium,
and Bdellovibrio. The relative mRNA expression levels of the
mTOR signaling-related genes TOR, 4E-BP, eIF4E1α, and
eIF4E2 all increased in the three PHB treatment groups. These results revealed that dietary PHB supplementation had a
beneficial effect on intestine health of L. vannamei by modulating
the composition of intestine microbiota and activating
mTOR signaling.
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Brassica rapa (Chinese cabbage) is an essential component
of traditional Korean food. However, the crop is often subject
to zinc (Zn+) toxicity from contaminated irrigation water,
which, as a result, compromises plant growth and production,
as well as the health of human consumers. The present study
investigated the bioaccumulation of Zn+ by Burkholderia cepacia
CS2-1 and its effect on the heavy metal tolerance of
Chinese cabbage. Strain CS2-1 was identified and characterized
on the basis of 16S rRNA sequences and phylogenetic
analysis. The strain actively produced indole-3-acetic acid
(3.08 ± 0.21 μg/ml) and was also able to produce siderophore,
solubilize minerals, and tolerate various concentrations of Zn+.
The heavy metal tolerance of B. rapa plants was enhanced
by CS2-1 inoculation, as indicated by growth attributes, Zn+
uptake, amino acid synthesis, antioxidant levels, and endogenous
hormone (ABA and SA) synthesis. Without inoculation,
the application of Zn+ negatively affected the growth and
physiology of B. rapa plants. However, CS2-1 inoculation
improved plant growth, lowered Zn+ uptake, altered both
amino acid regulation and levels of flavonoids and phenolics,
and significantly decreased levels of superoxide dismutase,
endogenous abscisic acid, and salicylic acid. These findings
indicate that B. cepacia CS2-1 is suitable for bioremediation
against Zn+-induced oxidative stress.
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In Salmonella enterica serovar Typhimurium, the acid-sensing
regulator CadC activates transcription of the cadBA operon
which contributes to the acid tolerance response. The
DNA-binding response regulator OmpR in two-component
regulatory system with EnvZ binds to its own promoter for
autoinduction. We previously reported that CadC exerts a
negative influence on ompR transcription during acid adaptation.
However, its underlying mechanisms remain to be elucidated.
Here we show that the level of OmpR protein is gradually
reduced by a gradual increase in the CadC level using
an arabinose-inducible expression system, indicating there
exists a negative correlation between the expression levels of
two transcription factors. To explore the molecular basis for
OmpR repression by CadC, we performed in vitro binding assays
and determined that CadC directly interacts with OmpR.
We further show that inactivation of cadC inhibits transcription
of the fliC gene, which encodes the major flagellar subunit, result ing in impaired flagellar motility under acid-adaptation
conditions. Together, our findings suggest that CadC may
repress autoinduction of the OmpR response regulator through
their direct interaction.
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The vibriocidal assay using guinea pig complement is widely
used for the evaluation of immune responses to cholera vaccines
in human clinical trials. However, it is unclear why
guinea pig complement has been used over human complement
in the measurement of vibriocidal activity of human
sera and there have not been comparison studies for the use
of guinea pig complement over those from other species.
Therefore, we comparatively investigated the effects of complements
derived from human, guinea pig, rabbit, and sheep
on vibriocidal activity. Complements from guinea pig, rabbit,
and human showed concentration-dependent vibriocidal activity
in the presence of quality control serum antibodies. Of
these complements, guinea pig complement was the most sensitive
and effective over a wide concentration range. When
the vibriocidal activity of complements was measured in the
absence of serum antibodies, human, sheep, and guinea pig
complements showed vibriocidal activity up to 40-fold, 20-
fold, and 1-fold dilution, respectively. For human pre- and
post-vaccination sera, the most potent vibriocidal activity was
observed when guinea pig complement was used. In addition,
the highest fold-increases between pre- and post- vaccinated
sera were obtained with guinea pig complement. Furthermore,
human complement contained a higher amount
of V. cholerae- and its lipopolysaccharide-specific antibodies
than guinea pig complement. Collectively, these results suggest
that guinea pig complements are suitable for vibriocidal
assays due to their high sensitivity and effectiveness to human
sera.
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cause high morbidity and mortality worldwide. Currently,
prophylactic vaccines and therapeutic antiviral agents are used
to prevent and control influenza virus infection. Oseltamivir
free base (OSV-FB), a modified generic antiviral drug of
Tamiflu (oseltamivir phosphate, OSV-P), was launched in
the Republic of Korea last year. Here, we examine the bioequivalence
of these two compounds by assessing their antiviral
efficacy in infected cells and in a mouse model. It was
observed that both antivirals showed comparable efficacy
against 11 different influenza A and B viruses in vitro. Moreover,
in mice infected with influenza A virus (mouse-adapted
A/Puerto Rico/8/34), they showed a dose-dependent therapeutic
activity and alleviated infection-mediated reductions
in body weight, leading to significantly better survival. There
was histopathological disappearance of virus-induced inflammatory
cell infiltration of the lung after oral treatment with
either antiviral agent (at 10 mg/kg). Pharmacokinetic analysis
also exhibited similar plasma concentrations of the active
drug, oseltamivir carboxylate, metabolised from both OSVB
and OSV-P. This is the first report showing bioequivalence
of OSV-FB to its phosphate salt form in the mouse system.
The free base drug has some beneficial points including simple
drug formulation process and reduced risk of undesirable
cation-phosphate precipitation within solution. The long term
stability of OSV-FB requires further monitoring when it is
provided as a national stock in readiness for an influenza
pandemic.
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Hepatitis E virus (HEV) is an etiological agent of acute hepatitis
E, a self-limiting disease prevalent in developing countries.
HEV can cause fulminant hepatic failure with high mortality
rates in pregnant women, and genotype 3 is reported to
trigger chronic hepatitis in immunocompromised individuals
worldwide. Screening of plant extracts for compounds with
potential anti-HEV effects led to the identification of a 70%
ethanol extract of Lysimachia mauritiana (LME) that interferes
with replication of the swine HEV genotype 3 replicon.
Furthermore, LME significantly inhibited replication of HEV
genotype 3 and expression of HEV ORF2 in infected cells
without exerting cytotoxic effects. Collectively, our findings
demonstrate the potential utility of LME in the development
of novel antiviral drugs against HEV infection.
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