Journal of Microbiology

Volume 53(4); April 2015

Review

Replicating poxviruses for human cancer therapy: Manbok Kim; J. Microbiol. 2015;53(4):209-218. Published online April 8, 2015; DOI: https://doi.org/10.1007/s12275-015-5041-4

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Naturally occurring oncolytic viruses are live, replicationproficient viruses that specifically infect human cancer cells while sparing normal cell counterparts. Since the eradication of smallpox in the 1970s with the aid of vaccinia viruses, the vaccinia viruses and other genera of poxviruses have shown various degrees of safety and efficacy in pre-clinical or clinical application for human anti-cancer therapeutics. Furthermore, we have recently discovered that cellular tumor suppressor genes are important in determining poxviral oncolytic tropism. Since carcinogenesis is a multi-step process involving accumulation of both oncogene and tumor suppressor gene abnormalities, it is interesting that poxvirus can exploit abnormal cellular tumor suppressor signaling for its oncolytic specificity and efficacy. Many tumor suppressor genes such as p53, ATM, and RB are known to play important roles in genomic fidelity/maintenance. Thus, tumor suppressor gene abnormality could affect host genomic integrity and likely disrupt intact antiviral networks due to accumulation of genetic defects, which would in turn result in oncolytic virus susceptibility. This review outlines the characteristics of oncolytic poxvirus strains, including vaccinia, myxoma, and squirrelpox virus, recent progress in elucidating the molecular connection between oncogene/tumor suppressor gene abnormalities and poxviral oncolytic tropism, and the associated preclinical/clinical implications. I would also like to propose future directions in the utility of poxviruses for oncolytic virotherapy.

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Research Support, Non-U.S. Gov't

New record and enzyme activity of four species in Penicillium section Citrina from marine environments in Korea: Myung Soo Park , Ji Eun Eom , Jonathan J. Fong , Young Woon Lim; J. Microbiol. 2015;53(4):219-225. Published online April 8, 2015; DOI: https://doi.org/10.1007/s12275-015-4700-9

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Abstract

Several strains of Penicillium section Citrina were isolated during a survey of fungi from marine environments along the southern coast of Korea. Based on multigene phylogenetic analyses (?tubulin and calmodulin) and morphological characteristics, the 11 strains were identified as P. citrinum, P. hetheringtonii, P. paxilli, P. sumatrense, P. terrigenum, and P. westlingii. To understand the ecological role of these species, we tested all strains for extracellular enzyme activity; six strains representing four species showed ?glucosidase activity. Four of the identified species ?P. hetheringtonii, P. paxilli, P. terrigenum, and P. westlingii ?are new records for Korea. For these new species records, we describe morphological characteristics of the strains and compare results to published data of type strains.

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Research Support, U.S. Gov't, Non-P.H.S.

Multiple cellular roles of Neurospora crassa plc-1, splA2, and cpe-1 in regulation of cytosolic free calcium, carotenoid accumulation, stress responses, and acquisition of thermotolerance§: Ananya Barman , Ranjan Tamuli; J. Microbiol. 2015;53(4):226-235. Published online January 31, 2015; DOI: https://doi.org/10.1007/s12275-015-4465-1

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Abstract

Phospholipase C1 (PLC1), secretory phospholipase A2 (sPLA2) and Ca2+/H+ exchanger proteins regulate calcium signaling and homeostasis in eukaryotes. In this study, we investigate functions for phospholipase C1 (plc-1), sPLA2 (splA2) and a Ca2+/H+ exchanger (cpe-1) in the filamentous fungus Neurospora crassa. The Δplc-1, ΔsplA2, and Δcpe-1 mutants exhibited a growth defect on medium supplemented with the divalent ionophore A23187, suggesting that these genes might play a role in regulation of cytosolic free Ca2+ concentration ([Ca2+]c) in N. crassa. The strains lacking plc-1, splA2, and cpe-1 possessed higher carotenoid content than wild type at 8°C, 22°C, and 30°C, and showed increased ultraviolet (UV)- survival under conditions that induced carotenoid accumulation. Moreover, Δplc-1, ΔsplA2, and Δcpe-1 mutants showed reduced survival rate under hydrogen peroxide-induced oxidative stress and induced thermotolerance after exposure to heat shock temperatures. Thus, this study revealed multiple cellular roles for plc-1, splA2, and cpe-1 genes in regulation of [Ca2+]c, carotenoid accumulation, survival under stress conditions, and acquisition of thermotolerance induced by heat shock.

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Research Support, Non-U.S. Gov'ts

Trichoderma reesei Sch9 and Yak1 regulate vegetative growth, conidiation, and stress response and induced cellulase production: Xinxing Lv† , Weixin Zhang† , Guanjun Chen , Weifeng Liu; J. Microbiol. 2015;53(4):236-242. Published online January 31, 2015; DOI: https://doi.org/10.1007/s12275-015-4639-x

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Abstract

Protein kinases are key players in controlling many basic cellular processes in almost all the organisms via mediating signal transduction processes. In the present study, we characterized the cellulolytic Trichoderma reesei orthologs of Saccharomyces cerevisiae Sch9 and Yak1 by sequence alignment and functional analysis. The T. reesei Trsch9Δ and Tryak1Δ mutant strains displayed a decreased growth rate on different carbon sources and produced less conidia. The absence of these two kinases also resulted in different but abnormal polarized apical growth as well as sensitivity to various stresses. In addition, disruption of the genes Trsch9 or Tryak1 resulted in perturbation of cell wall integrity. Interestingly, while the induced production of cellulases was slightly compromised in the Trsch9Δ strain, the extracellular production of cellulases was significantly improved in the absence of Yak1. The results indicate that TrSch9 and TrYak1 play an important role in filamentous growth, stress response and induced production of cellulases in T. reesei.

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Enhancing cellulase production in Neurospora crassa through combined deletion of the phospholipase D-encoding gene pla-7 and modulation of transcription factor CLR-2 expression
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Broad Substrate-Specific Phosphorylation Events Are Associated With the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa
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STK-12 acts as a transcriptional brake to control the expression of cellulase-encoding genes in Neurospora crassa
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Role of Trichoderma reesei mitogen-activated protein kinases (MAPKs) in cellulase formation
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The Aspergillus fumigatus SchASCH9 kinase modulates SakAHOG1 MAP kinase activity and it is essential for virulence
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Multiple roles of a putative vacuolar protein sorting associated protein 74, FgVPS74, in the cereal pathogen Fusarium graminearum: Hee-Kyoung Kim , Ki Woo Kim , Sung-Hwan Yun; J. Microbiol. 2015;53(4):243-249. Published online April 8, 2015; DOI: https://doi.org/10.1007/s12275-015-5067-7

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Abstract

Fusarium graminearum, a member of the F. graminearum species complex, is a filamentous ascomycetous group that causes serious diseases in cereal crops. A screen of insertional mutants of F. graminearum, generated using a restriction enzyme-mediated integration method, identified a mutant designated R7048 showing pleiotropic phenotypes in several mycological traits. The vector insertion site in the R7048 genome was identified as the KpnI site within an ORF annotated as FGSG_06346 (designated FgVPS74), which showed similarity to vacuolar protein sorting-associated protein 74 in the baker yeast. Both targeted gene deletion and complementation analyses confirmed that FgVPS74 was involved in hyphal growth, conidiation, sexual development, mycotoxin production, and virulence towards host plants in F. graminearum. Electron microscopy analysis revealed no significant changes in morphology of the vacuole or other organelles, but a greater number of mitochondria were produced in the ΔFgVPS74 strain compared to the wild-type progenitor. Expression of a GFP-tagged FgVPS74 construct under its native promoter in the ΔFgVPS74 strain exhibited localization of GFP signal to putative vesicle structures, but not to the vacuolar membrane. Taken together, these findings demonstrated that a functional vacuolar protein-sorting pathway mediated by FgVPS74 is crucial for fungal growth and development in F. graminearum.

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Sheetal M. Bhosle, Ragiba Makandar
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Photodynamic treatment with phenothiazinium photosensitizers kills both ungerminated and germinated microconidia of the pathogenic fungi Fusarium oxysporum, Fusarium moniliforme and Fusarium solani
Henrique Dantas de Menezes, Ludmilla Tonani, Luciano Bachmann, Mark Wainwright, Gilberto Úbida Leite Braga, Marcia Regina von Zeska Kress
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Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription: Hyung-Ju Lim , Kwangsoo Kim , Minsang Shin , Jae-Ho Jeong , Phil Youl Ryu , Hyon E. Choy; J. Microbiol. 2015;53(4):250-255. Published online April 8, 2015; DOI: https://doi.org/10.1007/s12275-015-4681-8

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Abstract

σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone- like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The
results
suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence.

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Sequence-dependent model of genes with dual σ factor preference
Ines S.C. Baptista, Vinodh Kandavalli, Vatsala Chauhan, Mohamed N.M. Bahrudeen, Bilena L.B. Almeida, Cristina S.D. Palma, Suchintak Dash, Andre S. Ribeiro
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms.2022; 1865(3): 194812. CrossRef
Function Enhancement of a Metabolic Module via Endogenous Promoter Replacement for Pseudomonas sp. JY-Q to Degrade Nicotine in Tobacco Waste Treatment
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Functional analysis of Vibrio vulnificus RND efflux pumps homologous to Vibrio cholerae VexAB and VexCD, and to Escherichia coli AcrAB: Seunghwa Lee , Ji-Hyun Yeom , Sojin Seo , Minho Lee , Sarang Kim , Jeehyeon Bae , Kangseok Lee , Jihwan Hwang; J. Microbiol. 2015;53(4):256-261. Published online March 4, 2015; DOI: https://doi.org/10.1007/s12275-015-5037-0

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Abstract

Resistance-nodulation-division (RND) efflux pumps are associated with multidrug resistance in many gram-negative pathogens. The genome of Vibrio vulnificus encodes 11 putative RND pumps homologous to those of Vibrio cholerae and Escherichia coli. In this study, we analyzed three putative RND efflux pumps, showing homology to V. cholerae VexAB and VexCD and to E. coli AcrAB, for their functional roles in multidrug resistance of V. vulnificus. Deletion of the vexAB homolog resulted in increased susceptibility of V. vulnificus to bile acid, acriflavine, ethidium bromide, and erythromycin, whereas deletion of acrAB homologs rendered V. vulnificus more susceptible to acriflavine only. Deletion of vexCD had no effect on susceptibility of V. vulnificus to these chemicals. Upon exposure to these antibacterial chemicals, expression of tolCV1 and tolCV2, which are putative outer membrane factors of RND efflux pumps, was induced, whereas expression levels of vexAB, vexCD, and acrAB homologs were not significantly changed. Our results show that the V. vulnificus homologs of VexAB largely contributed to in vitro antimicrobial resistance with a broad substrate specificity that was partially redundant with the AcrAB pump homologs.

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Sanath Kumar, Manjusha Lekshmi, Jerusha Stephen, Anely Ortiz-Alegria, Matthew Ayitah, Manuel F. Varela
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Jerusha Stephen, Manjusha Lekshmi, Parvathi Ammini, Sanath H. Kumar, Manuel F. Varela
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TolCV1 Has Multifaceted Roles During Vibrio vulnificus Infection
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ATP-Binding Cassette Transporter VcaM from Vibrio cholerae is Dependent on the Outer Membrane Factor Family for Its Function
Wen-Jung Lu, Hsuan-Ju Lin, Thamarai Janganan, Cheng-Yi Li, Wei-Chiang Chin, Vassiliy Bavro, Hong-Ting Lin
International Journal of Molecular Sciences.2018; 19(4): 1000. CrossRef
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Chung-Cheng Lo, Pei-Ting Lin, Chuan Chiang-Ni, Kuan-Hua Lin, Shin-Luen Lee, Tzu-Fang Kuo, Horng-Ren Lo
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Journal Article

Spectral characterization of a pteridine derivative from cyanide-utilizing bacterium Bacillus subtilis - JN989651: S. Durairaju Nisshanthini , Antony K. Teresa Infanta S. , Duraisamy Senthil Raja , Karuppannan Natarajan , M. Palaniswamy , Jayaraman Angayarkanni; J. Microbiol. 2015;53(4):262-271. Published online March 4, 2015; DOI: https://doi.org/10.1007/s12275-015-4138-0

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Abstract

Soil and water samples were collected from various regions of SIPCOT and nearby Vanappadi Lake, Ranipet, Tamilnadu, India. Based on their colony morphology and their stability during subculturing, 72 bacteria were isolated, of which 14 isolates were actinomycetes. Preliminary selection was carried out to exploit the ability of the microorganisms to utilize sodium cyanate as nitrogen source. Those organisms that were able to utilize cyanate were subjected to secondary screening viz., utilization of sodium cyanide as the nitrogen source. The oxygenolytic cleavage of cyanide is dependent on cyanide monooxygenase which obligately requires pterin cofactor for its activity. Based on this, the organisms capable of utilizing sodium cyanide were tested for the presence of pterin. Thin layer chromatography (TLC) of the cell extracts using n-butanol: 5 N glacial acetic acid (4:1) revealed that 10 out of 12 organisms that were able to utilize cyanide had the pterin-related blue fluorescent compound in the cell extract. The cell extracts of these 10 organisms were subjected to high performance thin layer chromatography (HPTLC) for further confirmation using a pterin standard. Based on the incubation period, cell biomass yield, peak height and area, strain VPW3 was selected and was identified as Bacillus subtilis. The Rf value of the cell extract was 0.73 which was consistent with the 0.74 Rf value of the pterin standard when scanned at 254 nm. The compound was extracted and purified by preparative High Performance Liquid Chromatography (HPLC). Characterization of the compound was performed by ultraviolet spectrum, fluorescence spectrum, Electrospray Ionization-Mass Spectrometry (ESI-MS), and Nuclear Magnetic Resonance spectroscopy (NMR). The compound is proposed to be 6-propionyl pterin (2-amino-6- propionyl-3H-pteridin-4-one).

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Cyanide Bioremediation by Bacillus subtilis under Alkaline Conditions
César Julio Cáceda Quiroz, Gabriela de Lourdes Fora Quispe, Milena Carpio Mamani, Gisela July Maraza Choque, Elisban Juani Sacari Sacari
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H. D. Douglas, I. V. Ermakov, W. Gellermann
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Microbial (Enzymatic) Degradation of Cyanide to Produce Pterins as Cofactors
Ramasamy Mahendran, Sabna BS, Murugesan Thandeeswaran, Kiran kG, Muthu Vijayasarathy, Jayaraman Angayarkanni, Gayathri Muthusamy
Current Microbiology.2020; 77(4): 578. CrossRef
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Production and optimization of pterin deaminase from cyanide utilizing bacterium Bacillus cereus AM12
Murugesan Thandeeswaran, Sajitha Bijukumar, Mani Arulkumar, Ramasamy Mahendran, Muthusamy Palaniswamy, Jayaraman Angayarkanni
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Evaluation of Pterin, a Promising Drug Candidate from Cyanide Degrading Bacteria
Ramasamy Mahendran, Murugesan Thandeeswaran, Gopikrishnan Kiran, Mani Arulkumar, K. A. Ayub Nawaz, Jayamanoharan Jabastin, Balraj Janani, Thomas Anto Thomas, Jayaraman Angayarkanni
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Analeptic agent from microbes upon cyanide degradation
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Research Support, Non-U.S. Gov'ts

Statistical experimental design optimization of rhamsan gum production by Sphingomonas sp. CGMCC 6833: Xiao-Ying Xu , Shu-Hao Dong , Sha Li , Xiao-Ye Chen , Ding Wu , Hong Xu; J. Microbiol. 2015;53(4):272-278. Published online April 8, 2015; DOI: https://doi.org/10.1007/s12275-015-3662-2

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Abstract

Rhamsan gum is a type of water-soluble exopolysaccharide produced by species of Sphingomonas bacteria. The optimal fermentation medium for rhamsan gum production by Sphingomonas sp. CGMCC 6833 was explored definition. Single-factor experiments indicate that glucose, soybean meal, K2HPO4 and MnSO4 compose the optimal medium along with and initial pH 7.5. To discover ideal cultural conditions for rhamsan gum production in a shake flask culture, response surface methodology was employed, from which the following optimal ratio was derived: 5.38 g/L soybean meal, 5.71 g/L K2HPO4 and 0.32 g/L MnSO4. Under ideal fermentation rhamsan gum yield reached 19.58 g/L ?1.23 g/L, 42.09% higher than that of the initial medium (13.78 g/L ? 1.38 g/L). Optimizing the fermentation medium results in enhanced rhamsan gum production.

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Hari Suthan Viswanathan, Sameeha Syed Abdul Rahman, Ponnusami Venkatachalam, Sugumaran Karuppiah
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Mervat A. Arayes, Mona E. M. Mabrouk, Soraya A. Sabry, Bahaa Abdella
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Effects of Different Agitator Blades on the Production Process of Biopolymer WL Gum by Sphingomonas sp. WG
Hui Li, Wanlong Zhou, Jiqian Wang, Hu Zhu
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Luis A. Trujillo-Cayado, Jenifer Santos, Nuria Calero, Maria del Carmen Alfaro, José Muñoz
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Hui Wang, Kun Jiang, Ziwei Zhu, Wankui Jiang, Zhangong Yang, Shijun Zhu, Jiguo Qiu, Xin Yan, Jian He, Qin He, Qing Hong
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Hui Li, Jing Li, Wanlong Zhou, Xue Jiao, Yajie Sun, Yaling Shen, Jin Qian, Jiqian Wang, Hu Zhu
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Peilian Wei, Zhenjun Si, Yao Lu, Qingfei Yu, Lei Huang, Zhinan Xu
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Directed analysis of cyanobacterial membrane phosphoproteome using stained phosphoproteins and titanium-enriched phosphopeptides§: Dong-Gi Lee , Joseph Kwon , Chi-Yong Eom , Young-Moon Kang , Seong Woon Roh , Kyung-Bok Lee , Jong-Soon Choi; J. Microbiol. 2015;53(4):279-287. Published online April 8, 2015; DOI: https://doi.org/10.1007/s12275-015-5021-8

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Abstract

Gel-free shotgun phosphoproteomics of unicellular cyanobacterium Synechocystis sp. PCC 6803 has not been reported up to now. The purpose of this study is to develop directed membrane phosphoproteomic method in Synechocystis sp. Total Synechocystis membrane proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and phosphoprotein-stained gel bands were selectively subjected to in-gel trypsin digestion. The phosphorylation sites of the resulting peptides were determined by assigning the neutral loss of [M-H3PO4] to Ser, Thr, and Tyr residues using nano-liquid chromatography 7 Tesla Fourier transform mass spectrometry. As an initial application, 111 proteins and 33 phosphoproteins were identified containing 11 integral membrane proteins. Identified four unknown phosphoproteins with transmembrane helices were suggested to be involved in membrane migration or transporters based on BLASTP search annotations. The overall distribution of hydrophobic amino acids in pTyr was lower in frequency than that of pSer or pThr. Positively charged amino acids were abundantly revealed in the surrounding amino acids centered on pTyr. A directed shotgun membrane phosphoproteomic strategy provided insight into understanding the fundamental regulatory processes underlying Ser, Thr, and Tyr phosphorylation in multi-layered membranous cyanobacteria.

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Rachna Agarwal, Julian P. Whitelegge, Sanjay Saini, Amit Prakash Shrivastav
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From the traditional Chinese medicine plant Schisandra chinensis new scaffolds effective on HIV-1 reverse transcriptase resistant to non-nucleoside inhibitors: Lijia Xu , Nicole Grandi , Claudia Del Vecchio , Daniela Mandas , Angela Corona , Dario Piano , Francesca Esposito , Cristina Parolin , Enzo Tramontano; J. Microbiol. 2015;53(4):288-293. Published online March 4, 2015; DOI: https://doi.org/10.1007/s12275-015-4652-0

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Abstract

HIV-1 reverse transcriptase (RT) is still an extremely attractive pharmaceutical target for the identification of new inhibitors possibly active on drug resistant strains. Medicinal plants are a rich source of chemical diversity and can be used to identify novel scaffolds to be further developed by chemical modifications. We investigated the ability of the main lignans from Schisandra chinensis (Turcz.) Baill. fruits, commonly used in Traditional Chinese Medicine, to affect HIV-1 RT functions. We purified 6 lignans from Schisandra chinensis fruits and assayed their effects on HIV-1 RT and viral replication. Among the S. chinensis fruit lignans, Schisandrin B and Deoxyschizandrin selectively inhibited the HIV-1 RTassociated DNA polymerase activity. Structure activity relationship revealed the importance of cyclooctadiene ring substituents for efficacy. In addition, Schisandrin B was also able to impair HIV-1 RT drug resistant mutants and the early phases of viral replication. We identified Schisandrin B and Deoxyschizandrin as new scaffold for the further development of novel HIV-1 RT inhibitors.

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Medicinal Chemistry Research.2019; 28(12): 2232. CrossRef
Schisandra chinensis and its phytotherapeutical applications
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Dual HIV-1 reverse transcriptase and integrase inhibitors from Limonium morisianum Arrigoni, an endemic species of Sardinia (Italy)
Cinzia Sanna, Daniela Rigano, Angela Corona, Dario Piano, Carmen Formisano, Domenica Farci, Genni Franzini, Mauro Ballero, Giuseppina Chianese, Enzo Tramontano, Orazio Taglialatela-Scafati, Francesca Esposito
Natural Product Research.2019; 33(12): 1798. CrossRef
Neuroprotective Effect of Schisandra Chinensis on Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Parkinsonian Syndrome in C57BL/6 Mice
Chi-Lin Li, Yang-Hwei Tsuang, Tung-Hu Tsai
Nutrients.2019; 11(7): 1671. CrossRef
Deoxyschizandrin, Isolated from Schisandra Berries, Induces Cell Cycle Arrest in Ovarian Cancer Cells and Inhibits the Protumoural Activation of Tumour-Associated Macrophages
Kijun Lee, Ji-Hye Ahn, Kyung-Tae Lee, Dae Jang, Jung-Hye Choi
Nutrients.2018; 10(1): 91. CrossRef
Pharmacokinetics of Schizandrin and Its Pharmaceutical Products Assessed Using a Validated LC–MS/MS Method
Chi-Lin Li, Yung-Yi Cheng, Chen-Hsi Hsieh, Tung-Hu Tsai
Molecules.2018; 23(1): 173. CrossRef
Harnessing the Properties of Natural Products
Asmaa Boufridi, Ronald J. Quinn
Annual Review of Pharmacology and Toxicology.2018; 58(1): 451. CrossRef
Schisandra chinensis Fructus and Its Active Ingredients as Promising Resources for the Treatment of Neurological Diseases
Minyu Zhang, Liping Xu, Hongjun Yang
International Journal of Molecular Sciences.2018; 19(7): 1970. CrossRef
Targeted Lignan Profiling and Anti-Inflammatory Properties of Schisandra rubriflora and Schisandra chinensis Extracts
Agnieszka Szopa, Michał Dziurka, Angelika Warzecha, Paweł Kubica, Marta Klimek-Szczykutowicz, Halina Ekiert
Molecules.2018; 23(12): 3103. CrossRef
Identification of Myricetin as an Ebola Virus VP35–Double-Stranded RNA Interaction Inhibitor through a Novel Fluorescence-Based Assay
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Biochemistry.2018; 57(44): 6367. CrossRef
Onopordum illyricum L., a Mediterranean plant, as a source of anti HIV-1 compounds
C. Sanna, D. Rigano, P. Cortis, A. Corona, M. Ballero, C. Parolin, C. Del Vecchio, G. Chianese, E. Saccon, C. Formisano, E. Tramontano, F. Esposito
Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology.2018; 152(6): 1274. CrossRef
Prenylated phloroglucinols from Hypericum scruglii, an endemic species of Sardinia (Italy), as new dual HIV-1 inhibitors effective on HIV-1 replication
Cinzia Sanna, Monica Scognamiglio, Antonio Fiorentino, Angela Corona, Vittoria Graziani, Alessia Caredda, Pierluigi Cortis, Mariofilippo Montisci, Elisa Rita Ceresola, Filippo Canducci, Ferruccio Poli, Enzo Tramontano, Francesca Esposito, Chandravanu Dash
PLOS ONE.2018; 13(3): e0195168. CrossRef
Uvaria angolensis as a promising source of inhibitors of HIV-1 RT-associated RNA-dependent DNA polymerase and RNase H functions
Alvine Ngoutane Mfopa, Angela Corona, Kodjo Eloh, Enzo Tramontano, Aldo Frau, Fabrice Fekam Boyom, Pierluigi Caboni, Graziella Tocco
Natural Product Research.2018; 32(6): 640. CrossRef
Natural Product Kuwanon‐L Inhibits HIV‐1 Replication through Multiple Target Binding
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ChemBioChem.2017; 18(4): 374. CrossRef
Current knowledge of Schisandra chinensis (Turcz.) Baill. (Chinese magnolia vine) as a medicinal plant species: a review on the bioactive components, pharmacological properties, analytical and biotechnological studies
Agnieszka Szopa, Radosław Ekiert, Halina Ekiert
Phytochemistry Reviews.2017; 16(2): 195. CrossRef
Human Immunodeficiency Virus and Herbal Medicine
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Alternative and Complementary Therapies.2017; 23(2): 51. CrossRef
Schisandra lignans production regulated by different bioreactor type
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Journal of Biotechnology.2017; 247: 11. CrossRef
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Scientific Reports.2017;[Epub] CrossRef
Identification of active components in Yixinshu Capsule with protective effects against myocardial dysfunction on human induced pluripotent stem cell-derived cardiomyocytes by an integrative approach
Minyu Zhang, Hongwei Wu, Feifei Guo, Yangyang Yu, Junying Wei, Ya Geng, Shifeng Wang, Shiyou Li, Hongjun Yang
Molecular BioSystems.2017; 13(8): 1469. CrossRef
Sennoside A, derived from the traditional chinese medicine plant Rheum L., is a new dual HIV-1 inhibitor effective on HIV-1 replication
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Phytomedicine.2016; 23(12): 1383. CrossRef
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ChemBioChem.2016; 17(8): 683. CrossRef
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BioMed Research International.2016; 2016: 1. CrossRef
Studies on Cycloheptathiophene‐3‐carboxamide Derivatives as Allosteric HIV‐1 Ribonuclease H Inhibitors
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De novo transcriptome assembly of Schisandra chinensis Turcz. (Baill.)
Zhang qing-tian, Fan shu-tian, Yang yi-ming, Xu pei-lei, Ai jun
Genomics Data.2016; 10: 153. CrossRef
Kuwanon‐L as a New Allosteric HIV‐1 Integrase Inhibitor: Molecular Modeling and Biological Evaluation
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