The NOX/DUOX family of NADPH oxidases are transmembrane
proteins generating reactive oxygen species as their
primary enzymatic products. NADPH oxidase (NOX) 1–5
and Dual oxidase (DUOX) 1 and 2 are members of this family.
These enzymes have several biological functions including
immune defense, hormone biosynthesis, fertilization, cell proliferation
and differentiation, extracellular matrix formation
and vascular regulation. They are found in a variety of tissues
such as the airways, salivary glands, colon, thyroid gland and
lymphoid organs. The discovery of NADPH oxidases has drastically
transformed our view of the biology of reactive oxygen
species and oxidative stress. Roles of several isoforms including
DUOX1 and DUOX2 in host innate immune defense
have been implicated and are still being uncovered. DUOX
enzymes highly expressed in the respiratory and salivary gland
epithelium have been proposed as the major sources of hydrogen
peroxide supporting mucosal oxidative antimicrobial
defenses. In this review, we shortly present data on DUOX
discovery, structure and function, and provide a detailed, upto-
date summary of discoveries regarding antibacterial, antiviral,
antifungal, and antiparasitic functions of DUOX enzymes.
We also present all the literature describing the immune
functions of lactoperoxidase, an enzyme working in
partnership with DUOX to produce antimicrobial substances.
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A Gram-negative, yellow-pigmented bacterial strain, designated
IPC6T, was isolated from soil in an arid region of
Goyang-si (Gyeonggi-do, South Korea). Cells were strictly
aerobic, non-spore-forming, rod-shaped. The strain grew
within a temperature range of 10–42°C (optimum, 30°C)
and pH of 5.0–11.0 (optimum, pH 8.0) in the presence of
0–2% (w/v) NaCl. Phylogenetically, the novel strain was
closely related to members of the Lysobacter genus based on
16S rRNA sequence similarity, and showed the highest sequence
similarity to Lysobacter niastensis KACC 11588T
(98.5%). The predominant fatty acids were iso-C15:0, iso-C16:0,
and summed feature 9 (iso-C17:1 ω9c), with Q-8 identified
as the major ubiquinone. The polar lipid content included
diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol,
an unknown aminophospholipid, and an
unidentified phospholipid. DNA-DNA hybridization results
indicated that the strain IPC6T was distinct from Lysobacter
niastensis KACC 11588T (37.9 ± 0.14%), Lysobacter panacisoli
KACC 17502T (56.4 ± 0.13%), Lysobacter soli KCTC
22011T (8.1 ± 0.04%), Lysobacter gummosus KCTC 12132T
(9.6 ± 0.03%), and Lysobacter cavernae KCTC 42875T (37.5 ±
0.14%), respectively. The DNA G + C content of the novel
strain was 71.1 mol%. Based on the collective phenotypic,
genotypic and chemotaxonomic data, the IPC6T strain is considered
to represent a novel species in the genus Lysobacter,
for which the name Lysobacter pedocola sp. nov. (= KCTC
42811T = JCM 31020T) is proposed.
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A rod-shaped, white color colony with lobate architectures,
strain h2T was isolated from a moderately acidic soil on Jeju
Island, Republic of Korea. Comparative analysis of the 16S
rRNA gene sequence showed that the strain h2T is closely
related to Paenibacillus relictisesami DSM 25385T (97.4%,
16S rRNA gene sequence similarity), Paenibacillus azoreducens
KACC 11244T (97.2%), and Paenibacillus cookii LMG
18419T (97.0%). DNA-DNA hybridization indicated that the
strain h2T has relatively low levels of DNA-DNA relatedness
with respect to P. relictisesami DSM 25385T (10.2%) and P.
azoreducens KACC 11244T (13.7%). Additionally, the genomic
DNA G + C content of h2T is 51.5 mol%. The isolated strain
grew at pH 4.0–9.0 (optimum, pH 6.0–7.0) and 0–5% (w/v)
NaCl (optimum, 0%) and a temperature of 15–45°C (optimum
35°C). The quinones in the strain are MK-6 and MK-7,
and the predominant fatty acid is C15:0 anteiso (32.1%) followed
by C17:0 anteiso (26.5%), and C16:0 iso (21.0%). Based
on its phenotypic properties, genotypic distinctiveness, and
chemotaxonomic features, strain h2T is proposed as a novel
species in the genus Paenibacillus, for which the name Paenibacillus
albilobatus sp. nov. is proposed (= KCCM 43269T =
JCM 32395T = LMG 30408T). The type strain of Paenibacillus
albilobatus is h2T.
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Tricholoma matsutake is an ectomycorrhizal fungus usually
associated with Pinus densiflora in South Korea. Fruiting
bodies (mushrooms) of T. matsutake are economically important
due to their attractive aroma; yet, T. matsutake is
uncultivatable and its habitat is rapidly being eradicated due
to global climate change. Root-associated bacteria can influence
the growth of ectomycorrhizal fungi that co-exist in the
host rhizosphere and distinctive bacterial communities are
associated with T. matsutake. In this study, we investigated
how these bacterial communities affect T. matsutake growth
by isolating bacteria from the roots of P. densiflora colonized
by ectomycorrhizae of T. matsutake and co-culturing rootassociated
bacteria with T. matsutake isolates. Thirteen species
of bacteria (27 isolates) were found in pine roots, all
belonging to the orders Bacillales or Burkholderiales. Two
species in the genus Paenibacillus promoted the growth of
T. matsutake in glucose poor conditions, likely using soluble
metabolites. In contrast, other bacteria suppressed the growth
of T. matsutake using both soluble and volatile metabolites.
Antifungal activity was more frequent in glucose poor conditions.
In general, pine rhizospheres harbored many bacteria
that had a negative impact on T. matsutake growth and the
few Paenibacillus species that promoted T. matsutake growth.
Paenibacillus species, therefore, may represent a promising
resource toward successful cultivation of T. matsutake.
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The increased antibiotic resistance among microorganisms
has resulted into growing interest for investigating the wastewater
treatment plants (WWTPs) as they are reported to be
the major source in the dissemination of antibiotic resistance
genes (ARGs) and heavy metal resistance genes (HMRGs)
in the environment. In this study, we investigated the prevalence
and persistence of ARGs and HMRGs as well as bacterial
diversity and mobile genetic elements (MGEs) in influent
and effluent at the WWTP in Gwangju, South Korea,
using high-throughput sequencing based metagenomic approach.
A good number of broad-spectrum of resistance
genes (both ARG and HMRG) were prevalent and likely
persistent, although large portion of them were successfully
removed at the wastewater treatment process. The relative
abundance of ARGs and MGEs was higher in effluent as compared
to that of influent. Our results suggest that the resistance
genes with high abundance and bacteria harbouring
ARGs and MGEs are likely to persist more through the treatment
process. On analyzing the microbial community, the
phylum Proteobacteria, especially potentially pathogenic species
belonging to the genus Acinetobacter, dominated in
WWTP. Overall, our study demonstrates that many ARGs
and HMRGs may persist the treatment processes in WWTPs
and their association to MGEs may contribute to the dissemination
of resistance genes among microorganisms in the
environment.
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Diversity of A mating type in Lentinula edodes has been assessed
by analysis of A mating loci in 127 strains collected
from East Asia. It was discovered that hypervariable sequence
region with an approximate length of 1 kb in the A mating
locus, spanning 5region of HD2-intergenic region-5region
of HD1, could represent individual A mating type as evidenced
by comprehensive mating analysis. The sequence analysis
revealed 27 A mating type alleles from 96 cultivated
strains and 48 alleles from 31 wild strains. Twelve of them
commonly appeared, leaving 63 unique A mating type alleles.
It was also revealed that only a few A mating type alleles such
as A1, A4, A5, and A7 were prevalent in the cultivated strains,
accounting for 62.5% of all A mating types. This implies
preferred selection of certain A mating types in the process
of strain development and suggests potential role of A mating
genes in the expression of genes governing mushroom
quality. Dominant expression of an A mating gene HD1 was
observed from A1 mating locus, the most prevalent A allele,
in A1-containing dikaryons. However, connections between
HD1 expression and A1 preference in the cultivated strains
remain to be verified. The A mating type was highly diverse
in the wild strains. Thirty-six unique A alleles were discovered
from relatively small and confined area of mountainous region
in Korean peninsula. The number will further increase
because no A allele has been recurrently observed in the wild
strains and thus newly discovered strain will have good chances
to contain new A allele. The high diversity in small area
also suggests that the A mating locus has evolved rapidly
and thus its diversity will further increase.
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The freshwater green algae Closterium is sensitive to water
quality, and hence has been suggested as ideal organisms for
toxicity testing. In the present study, we evaluated the photosynthetic
and biochemical responses of C. ehrenbergii to
the common contaminants, coppers. The 72 h median effective
concentrations (EC50) of CuSO4 and CuCl2 on the test
organism were calculated to be 0.202 mg/L and 0.245 mg/L,
respectively. Exposure to both coppers considerably decreased
pigment levels and photosynthetic efficiency, while inducing
the generation of reactive oxygen species (ROS) in cells
with increased exposure time. Moreover, the coppers significantly
increased the levels of lipid peroxidation and superoxide
dismutase (SOD) activity, even at relatively lower concentrations.
These suggest that copper contaminants may
exert deleterious effects on the photosynthesis and cellular
oxidative stress of C. ehrenbergii, representing its powerful
potential in aquatic toxicity assessments.
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We investigated the colistin resistance rate among 356 Enterobacter
spp. clinical isolates from eight hospitals in Korea.
Antibiotic susceptibility testing was performed by broth microdilution.
While 51 of 213 (23.9%) Enterobacter cloacae isolates
were colistin-resistant, only six of 143 (4.2%) E. aerogenes
isolates showed resistance. We also identified the skip
well phenotype in eight E. cloacae and three E. aerogenes
isolates. Multilocus sequence typing for E. cloacae and randomly
amplified polymorphic DNA analysis and enterobacterial
repetitive intergenic consensus PCR for E. aerogenes
revealed that clonal spreading of colistin-resistant and skip
well Enterobacter spp. isolates had not occurred. In vitro
time-kill assays were performed with three colistin-resistant,
three skip well, and two colistin-susceptible isolates of E. cloacae
and E. aerogenes. Inconsistent results were observed
among isolates with skip well phenotypes; while some were
eradicated by 2 mg/L colistin, others were not. This suggests
that skip well isolates have differentiated into different categories.
As the high rates of colistin resistance in E. cloacae
detected are of clinical concern, continuous monitoring is
warranted. In addition, the clinical implications and mechanisms
of the skip well phenotype should be investigated to
ensure the appropriate use of colistin against Enterobacter
infections.
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Live attenuated vaccine strains have been developed for Varicella-
Zoster virus (VZV). Compared to clinically isolated
strains, the vaccine strains contain several non-synonymous
mutations in open reading frames (ORFs) 0, 6, 31, 39, 55, 62,
and 64. In particular, ORF62, encoding an immediate-early
(IE) 62 protein that acts as a transactivator for viral gene
expression, contains six non-synonymous mutations, but
whether these mutations affect transactivation activity of
IE62 is not understood. In this study, we investigated the
role of non-synonymous vaccine-type mutations (M99T,
S628G, R958G, V1197A, I1260V, and L1275S) of IE62 in
Suduvax, a vaccine strain isolated in Korea, for transactivation
activity. In reporter assays, Suduvax IE62 showed 2- to
4-fold lower transactivation activity toward ORF4, ORF28,
ORF29, and ORF68 promoters than wild-type IE62. Introduction
of individual M99T, S628G, R958G, or V1197A/
I1260V/L1275S mutations into wild-type IE62 did not affect
transactivation activity. However, the combination of M99T
within the N-terminal Sp transcription factor binding region
and V1197A/I1260V/L1275S within the C-terminal serineenriched
acidic domain (SEAD) significantly reduced the
transactivation activity of IE62. The M99T/V1197A/I1260V/
L1275S mutant IE62 did not show considerable alterations
in intracellular distribution and Sp3 binding compared to
wild-type IE62, suggesting that other alteration(s) may be
responsible for the reduced transactivation activity. Collectively,
our results suggest that acquisition of mutations in
both Met 99 and the SEAD of IE62 is responsible for the reduced
transactivation activity found in IE62 of the VZV
vaccine strains and contributes to attenuation of the virus.
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