Journal of Microbiology

Journal Article

Environmental Adaptation of Psychrophilic Bacteria Subtercola spp. Isolated from Various Cryospheric Habitats: Hanbyul Lee , Yong-Joon Cho , Ahnna Cho , Ok-Sun Kim; J. Microbiol. 2023;61(7):663-672. Published online August 24, 2023; DOI: https://doi.org/10.1007/s12275-023-00068-y

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Abstract: Subtercola boreus K300T is a novel psychrophilic strain that was isolated from permanently cold groundwater in Finland and has also been found in several places in Antarctica including lake, soil, and rocks. We performed genomic and transcriptomic analyses of 5 strains from Antarctica and a type strain to understand their adaptation to different environments. Interestingly, the isolates from rocks showed a low growth rate and smaller genome size than strains from the other isolation sources (lake, soil, and groundwater). Based on these habitat-dependent characteristics, the strains could be classified into two ecotypes, which showed differences in energy production, signal transduction, and transcription in the clusters of orthologous groups of proteins (COGs) functional category. In addition, expression pattern changes revealed differences in metabolic processes, including uric acid metabolism, DNA repair, major facilitator superfamily (MFS) transporters, and xylose degradation, depending on the nutritional status of their habitats. These findings provide crucial insights into the environmental adaptation of bacteria, highlighting genetic diversity and regulatory mechanisms that enable them to thrive in the cryosphere.

Review

[MINIREVIEW]Gain and loss of antibiotic resistant genes in multidrug resistant bacteria: One Health perspective: Misung Kim , Jaeeun Park , Mingyeong Kang , Jihye Yang , Woojun Park; J. Microbiol. 2021;59(6):535-545. Published online April 20, 2021; DOI: https://doi.org/10.1007/s12275-021-1085-9

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The emergence of multidrug resistance (MDR) has become a global health threat due to the increasing unnecessary use of antibiotics. Multidrug resistant bacteria occur mainly by accumulating resistance genes on mobile genetic elements (MGEs), made possible by horizontal gene transfer (HGT). Humans and animal guts along with natural and engineered environments such as wastewater treatment plants and manured soils have proven to be the major reservoirs and hotspots of spreading antibiotic resistance genes (ARGs). As those environments support the dissemination of MGEs through the complex interactions that take place at the human-animalenvironment interfaces, a growing One Health challenge is for multiple sectors to communicate and work together to prevent the emergence and spread of MDR bacteria. However, maintenance of ARGs in a bacterial chromosome and/or plasmids in the environments might place energy burdens on bacterial fitness in the absence of antibiotics, and those unnecessary ARGs could eventually be lost. This review highlights and summarizes the current investigations into the gain and loss of ARG genes in MDR bacteria among human-animal- environment interfaces. We also suggest alternative treatments such as combinatory therapies or sequential use of different classes of antibiotics/adjuvants, treatment with enzymeinhibitors, and phage therapy with antibiotics to solve the MDR problem from the perspective of One Health issues.

Citations

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Science of The Total Environment.2024; 909: 168516. CrossRef
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Environmental Science and Pollution Research.2024; 31(36): 48813. CrossRef
Unexpected vulnerability of Enterococcus faecium to polymyxin B under anaerobic condition
Yongjun Son, Bitnara Kim, Pureun Kim, Jihyeon Min, Yerim Park, Jihye Yang, Wonjae Kim, Masanori Toyofuku, Woojun Park
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Pavithra Anantharaman Sudhakari, Bhaskar Chandra Mohan Ramisetty
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Meixia Huo, Xiangyue Xu, Kun Mi, Wenjin Ma, Qin Zhou, Xudong Lin, Guyue Cheng, Lingli Huang
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An Nguyen, J. Jean Sophy Roy, Ji-Hoon Kim, Kyung-Hee Yun, Wonsik Lee, Kyeong Kyu Kim, Truc Kim, Akhilesh Kumar Chaurasia
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Animals.2023; 13(19): 3083. CrossRef
Longitudinal study of the short- and long-term effects of hospitalisation and oral trimethoprim-sulfadiazine administration on the equine faecal microbiome and resistome
Mathijs J. P. Theelen, Roosmarijn E. C. Luiken, Jaap A. Wagenaar, Marianne M. Sloet van Oldruitenborgh-Oosterbaan, John W. A. Rossen, Femke J. W. C. Schaafstra, David A. van Doorn, Aldert L. Zomer
Microbiome.2023;[Epub] CrossRef
Genomic evidences of gulls as reservoirs of critical priority CTX-M-producing Escherichia coli in Corcovado Gulf, Patagonia
Danny Fuentes-Castillo, Daniela Castro-Tardón, Fernanda Esposito, Ingrith Neves, Larissa Rodrigues, Herrison Fontana, Bruna Fuga, José L. Catão-Dias, Nilton Lincopan
Science of The Total Environment.2023; 874: 162564. CrossRef
Poultry manure-derived microorganisms as a reservoir and source of antibiotic resistance genes transferred to soil autochthonous microorganisms
Magdalena Męcik, Martyna Buta-Hubeny, Łukasz Paukszto, Mateusz Maździarz, Izabela Wolak, Monika Harnisz, Ewa Korzeniewska
Journal of Environmental Management.2023; 348: 119303. CrossRef
Fabrication of Co-Assembly from Berberine and Tannic Acid for Multidrug-Resistant Bacteria Infection Treatment
Tingting Zheng, Huan Chen, Chenyang Wu, Jinrui Wang, Mengyao Cui, Hanyi Ye, Yifan Feng, Ying Li, Zhengqi Dong
Pharmaceutics.2023; 15(7): 1782. CrossRef
Grazing disturbance increased the mobility, pathogenicity and host microbial species of antibiotic resistance genes, and multidrug resistance genes posed the highest risk in the habitats of wild animals
Shuzhen Zou, Tan Lu, Cailiang Huang, Jie Wang, Dayong Li
Frontiers in Environmental Science.2023;[Epub] CrossRef
Application of electrochemical oxidation for the enhancement of antibiotic resistant bacteria removal in stormwater bioretention cells
XiaoJun Zuo, SongHu Zhang, FanXin Kong, QiangQiang Xu
Science of The Total Environment.2023; 861: 160477. CrossRef
Blue Light Sensing BlsA-Mediated Modulation of Meropenem Resistance and Biofilm Formation in Acinetobacter baumannii
Jihye Yang, Sohyeon Yun, Woojun Park, Mark J. Mandel
mSystems.2023;[Epub] CrossRef
AamA-mediated epigenetic control of genome-wide gene expression and phenotypic traits in Acinetobacter baumannii ATCC 17978
Jihye Yang, Yongjun Son, Mingyeong Kang, Woojun Park
Microbial Genomics .2023;[Epub] CrossRef
Response behavior of antibiotic resistance genes and human pathogens to slope gradient and position: An environmental risk analysis in sloping cultivated land
Kailin Xu, Xuna Liu, Lina Pang, Yao Yue, Efthalia Chatzisymeon, Ping Yang
Science of The Total Environment.2023; 905: 166994. CrossRef
The β-Lactamase Activity at the Community Level Confers β-Lactam Resistance to Bloom-Forming Microcystis aeruginosa Cells
Yerim Park, Wonjae Kim, Minkyung Kim, Woojun Park
Journal of Microbiology.2023; 61(9): 807. CrossRef
Bactericidal activity of silver nanoparticles in drug-resistant bacteria
C. Chapa González, L. I. González García, L. G. Burciaga Jurado, A. Carrillo Castillo
Brazilian Journal of Microbiology.2023; 54(2): 691. CrossRef
Computational modelling of epithelial cell monolayers during infection with Listeria monocytogenes
Raul Aparicio-Yuste, Francisco Serrano-Alcalde, Marie Muenkel, Jose Manuel Garcia-Aznar, Effie E. Bastounis, Maria Jose Gomez-Benito
Computer Methods in Applied Mechanics and Engineering.2022; 401: 115477. CrossRef
Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice
Jaeyeong Park, Eunkyoung Shin, Ji-Hyun Yeom, Younkyung Choi, Minju Joo, Minho Lee, Je Hyeong Kim, Jeehyeon Bae, Kangseok Lee
Journal of Microbiology.2022; 60(1): 128. CrossRef
Antibiotic resistome in a large urban-lake drinking water source in middle China: Dissemination mechanisms and risk assessment
Maozhen Han, Lu Zhang, Na Zhang, Yujie Mao, Zhangjie Peng, Binbin Huang, Yan Zhang, Zhi Wang
Journal of Hazardous Materials.2022; 424: 127745. CrossRef
Occurrence of antibiotic resistance genes and multidrug-resistant bacteria during wastewater treatment processes
Mingyeong Kang, Jihye Yang, Suhyun Kim, Jaeeun Park, Misung Kim, Woojun Park
Science of The Total Environment.2022; 811: 152331. CrossRef
Occurrence and spread of antibiotic-resistant bacteria on animal farms and in their vicinity in Poland and Ukraine—review
Karolina Jeżak, Anna Kozajda
Environmental Science and Pollution Research.2022; 29(7): 9533. CrossRef
Application of Electrochemical Oxidation for the Enhancement of Antibiotic Resistant Bacteria Removal in Stormwater Bioretention Cells
XiaoJun Zuo, SongHu Zhang, FanXin Kong, QiangQiang Xu
SSRN Electronic Journal .2022;[Epub] CrossRef
Exploration of the presence and abundance of multidrug resistance efflux genes in oil and gas environments
Damon C. Brown, Naomi Aggarwal, Raymond J. Turner
Microbiology .2022;[Epub] CrossRef
Small Things Matter: The 11.6-kDa TraB Protein is Crucial for Antibiotic Resistance Transfer Among Enterococci
Tamara M.I. Berger, Claudia Michaelis, Ines Probst, Theo Sagmeister, Lukas Petrowitsch, Sandra Puchner, Tea Pavkov-Keller, Bernd Gesslbauer, Elisabeth Grohmann, Walter Keller
Frontiers in Molecular Biosciences.2022;[Epub] CrossRef
The microbiome and its association with antibiotic resistance genes in the hadal biosphere at the Yap Trench
Hualong Su, Chengcheng Wu, Peiyun Han, Zixuan Liu, Mincong Liang, Zheng Zhang, Zhike Wang, Guangyu Guo, Xinyi He, Jianhu Pang, Cheng Wang, Shaoping Weng, Jianguo He
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Journal Articles

Anti-inflammatory and anti-oxidative effect of Korean propolis on Helicobacter pylori-induced gastric damage in vitro: Moon-Young Song , Da-Young Lee , Eun-Hee Kim; J. Microbiol. 2020;58(10):878-885. Published online September 2, 2020; DOI: https://doi.org/10.1007/s12275-020-0277-z

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Abstract

Helicobacter pylori, present in the stomach lining, is a Gramnegative bacterium that causes various gastrointestinal diseases, including gastritis and peptic ulcers. Propolis is a natural resinous substance collected from a variety of plants, and contains several natural bioactive substances. The aim of this study was to investigate the anti-inflammatory and antioxidative effects of Korean propolis on H. pylori-induced damage in the human adenocarcinoma gastric cell line. The propolis used in this study was obtained from the Korea Beekeeping Association in South Korea. The expression of pro-inflammatory interleukins (ILs), such as IL-8, IL-12, IL-1β, tumor necrosis factor alpha, cyclooxygenase-2, and inducible nitric oxide synthase, which was increased after H. pylori infection, significantly decreased in a dose-dependent manner upon pretreatment with Korean propolis, because of the suppression of mitogen-activated protein kinases and nuclear factor κB pathway. The anti-oxidative activity of propolis was assessed using the 2,2-diphenyl-1-picrylhydrazyl hydrate free radical assay. Korean propolis showed significant anti-oxidative effects via reactive oxygen species scavenging. In addition, pretreatment with Korean propolis upregulated the expression of anti-oxidant enzymes through Nrf2 signaling activation. These findings indicate that the use of Korean propolis, which has anti-inflammatory and anti-oxidative effects, can be promising for the prevention of H. pylori-induced gastric damage.

Citations

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Network Pharmacology and Experimental Validation Reveal Therapeutic Potential of Propolis in UV-Induced Allergic Dermatitis
Liyuan Cheng, Jie Wang, Yicong Wang, Jingjing Li, Wenchao Yang
Foods.2025; 14(6): 996. CrossRef
Propolis as an autophagy modulator in relation to its roles in redox balance and inflammation regulation
R. Lesmana, S. Tandean, A. Christoper, A.A. Suwantika, N. Wathoni, R. Abdulah, J. Fearnley, V. Bankova, F. Zulhendri
Biomedicine & Pharmacotherapy.2024; 175: 116745. CrossRef
Exploring the Prospective Role of Propolis in Modifying Aging Hallmarks
Carla Scorza, Valeria Goncalves, Josef Finsterer, Fúlvio Scorza, Fernando Fonseca
Cells.2024; 13(5): 390. CrossRef
Development of a chitosan/propolis-based polymeric system: Characterization, biocompatibility, and modulation of transcription factor expression
Raquel Velázquez-Rodríguez, Amaury Pozos-Guillén, Martha Gabriela Chuc-Gamboa, Juan Valerio Cauich-Rodríguez, Héctor Flores-Reyes, Francisco Javier Tejeda-Nava, Fernando Javier Aguilar-Perez, Diana Maria Escobar Garcia
Journal of Bioactive and Compatible Polymers.2024; 39(5): 329. CrossRef
Therapeutic potential of propolis in alleviating inflammatory response and promoting wound healing in skin burn
Christian Oktavianus Manginstar, Trina Ekawati Tallei, Nurdjannah Jane Niode, Christina Leta Salaki, Sofia Safitri Hessel
Phytotherapy Research.2024; 38(2): 856. CrossRef
A review for non-antibiotic treatment of Helicobacter pylori: new insight
Neda Shadvar, Sousan Akrami, Seyyed-Mohammad-Amin Mousavi Sagharchi, Rafee Habib Askandar, Alireza Merati, Masoomeh Aghayari, Nikki Kaviani, Hamed Afkhami, Mojtaba Kashfi
Frontiers in Microbiology.2024;[Epub] CrossRef
Wound Healing, Anti-Inflammatory and Anti-Oxidant Activities, and Chemical Composition of Korean Propolis from Different Sources
Aman Dekebo, Chalshisa Geba, Daniel Bisrat, Jin Boo Jeong, Chuleui Jung
International Journal of Molecular Sciences.2024; 25(21): 11352. CrossRef
Effects of oxidative stress regulation in inflammation-associated gastric cancer progression treated using traditional Chinese medicines: A review
Bo Chen, Xinqian Dong, Jinlong Zhang, Wei Wang, Yujiao Song, Xitong Sun, Kangning Zhao, Zhen Sun
Medicine.2023; 102(46): e36157. CrossRef
Emodin Attenuates Inflammasome Activation Induced by Helicobacter pylori Infection through Inhibition of VacA Translocation
Thach Phan Van, Anh Duy Do
Microbiology and Biotechnology Letters.2023; 51(4): 507. CrossRef
Correlation between Chemical Profile of Georgian Propolis Extracts and Their Activity against Helicobacter pylori
Jarosław Widelski, Piotr Okińczyc, Katarzyna Suśniak, Anna Malm, Anna Bozhadze, Malkhaz Jokhadze, Izabela Korona-Głowniak
Molecules.2023; 28(3): 1374. CrossRef
Therapeutic effect of propolis nanoparticles on wound healing
Juan Yang, Yingjuan He, Sha Nan, Juan Li, Anjuan Pi, Lele Yan, Jinshan Xu, Yuhui Hao
Journal of Drug Delivery Science and Technology.2023; 82: 104284. CrossRef
Recent Update on the Anti-Inflammatory Activities of Propolis
Felix Zulhendri, Ronny Lesmana, Steven Tandean, Andreas Christoper, Kavita Chandrasekaran, Ilham Irsyam, Auliya A. Suwantika, Rizky Abdulah, Nasrul Wathoni
Molecules.2022; 27(23): 8473. CrossRef
Construction of a Cuprotosis-Related Gene-Based Model to Improve the Prognostic Evaluation of Patients with Gastric Cancer
Chunyan Han, Kai Zhang, XinKai Mo, Fu Wang
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Honey Bee Products: Preclinical and Clinical Studies of Their Anti-inflammatory and Immunomodulatory Properties
Hesham R. El-Seedi, Nehal Eid, Aida A. Abd El-Wahed, Mostafa E. Rateb, Hanan S. Afifi, Ahmed F. Algethami, Chao Zhao, Yahya Al Naggar, Sultan M. Alsharif, Haroon Elrasheid Tahir, Baojun Xu, Kai Wang, Shaden A. M. Khalifa
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María Guerra-Valle, Patricio Orellana-Palma, Guillermo Petzold
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DETERMINATION OF ANTI-UREASE ACTIVITY OF PROPOLIS FROM MARMARA REGION OF TURKEY
Zehra Can, Yakup Kara, Sevgi Kolaylı, İbrahim Çakmak
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Nrf2 Downregulation Contributes to Epithelial-to-Mesenchymal Transition in Helicobacter pylori-Infected Cells
Sarah Bacon, Lornella Seeneevassen, Alison Fratacci, Faustine Rose, Camille Tiffon, Elodie Sifré, Maria M. Haykal, Maya M. Moubarak, Astrid Ducournau, Lucie Bruhl, Stéphane Claverol, Caroline Tokarski, Alina-Roxani Gouloumi, Ioannis S. Pateras, Thomas Dau
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Anti-Inflammatory Effect of Korean Propolis on Helicobacter pylori-Infected Gastric Mucosal Injury Mice Model
Moon-Young Song, Da-Young Lee, Young-Min Han, Eun-Hee Kim
Nutrients.2022; 14(21): 4644. CrossRef
Antifungal Properties of Chemically Defined Propolis from Various Geographical Regions
Marcin Ożarowski, Tomasz M. Karpiński, Rahat Alam, Małgorzata Łochyńska
Microorganisms.2022; 10(2): 364. CrossRef
The Antimicrobial Properties of Poplar and Aspen–Poplar Propolises and Their Active Components against Selected Microorganisms, including Helicobacter pylori
Jarosław Widelski, Piotr Okińczyc, Emil Paluch, Tomasz Mroczek, Jakub Szperlik, Magdalena Żuk, Zbigniew Sroka, Zuriyadda Sakipova, Ioanna Chinou, Krystyna Skalicka-Woźniak, Anna Malm, Izabela Korona-Głowniak
Pathogens.2022; 11(2): 191. CrossRef
Research Progress on Therapeutic Effect and Mechanism of Propolis on Wound Healing
Juan Yang, Anjuan Pi, Lele Yan, Juan Li, Sha Nan, Jing Zhang, Yuhui Hao, Juraj Majtan
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"GREEN SYNTHESIS OF NANO-SILVER/SODIUM ALGINATE/CARBOXYMETHYL XANTHAN GUM HYDROGEL AND EVALUATION OF ITS ANTI-INFLAMMATORY AND ANTI-Helicobacter pylori ACTIVITY "
NOURA Y. ELMEHBAD, NADIA A. MOHAMED, NAHED A. ABD EL-GHANY, MARWA M. ABDEL-AZIZ
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Propolis-Loaded Chitosan Nanoparticles for the Treatment of Bacterial Diarrhea: In Vitro and In Vivo Evaluation
Jie Qiao, Jun Yang, Saman Jalili
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Steamed Ginger Extract Exerts Anti-inflammatory Effects in Helicobacter pylori-infected Gastric Epithelial Cells through Inhibition of NF-κB
Moon-Young Song, Da-Young Lee, Sang-Yong Park, Seul-A Seo, Jeong-Seung Hwang, Soo-Hyeon Heo, Eun-Hee Kim
Journal of Cancer Prevention.2021; 26(4): 289. CrossRef
Short‐term efficacy of a gel containing propolis extract, nanovitamin C and nanovitamin E on peri‐implant mucositis: A double‐blind, randomized, clinical trial
José González‐Serrano, Rosa María López‐Pintor, Julia Serrano, Jesús Torres, Gonzalo Hernández, Mariano Sanz
Journal of Periodontal Research.2021; 56(5): 897. CrossRef
Experimental Evidence for Therapeutic Potentials of Propolis
Priyanshu Bhargava, Debajit Mahanta, Ashish Kaul, Yoshiyuki Ishida, Keiji Terao, Renu Wadhwa, Sunil C. Kaul
Nutrients.2021; 13(8): 2528. CrossRef
Propolis and Its Gastroprotective Effects on NSAID-Induced Gastric Ulcer Disease: A Systematic Review
Porfirio Alonso Ruiz-Hurtado, Leticia Garduño-Siciliano, Pilar Domínguez-Verano, Daniela Balderas-Cordero, Gustavo Gorgua-Jiménez, Octavio Canales-Álvarez, María Margarita Canales-Martínez, Marco Aurelio Rodríguez-Monroy
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Molecular Insights into the Antistress Potentials of Brazilian Green Propolis Extract and Its Constituent Artepillin C
Ashish Kaul, Raviprasad Kuthethur, Yoshiyuki Ishida, Keiji Terao, Renu Wadhwa, Sunil C. Kaul
Molecules.2021; 27(1): 80. CrossRef

The effects of deletion of cellobiohydrolase genes on carbon source-dependent growth and enzymatic lignocellulose hydrolysis in Trichoderma reesei: Meibin Ren , Yifan Wang , Guoxin Liu , Bin Zuo , Yuancheng Zhang , Yunhe Wang , Weifeng Liu , Xiangmei Liu , Yaohua Zhong; J. Microbiol. 2020;58(8):687-695. Published online June 10, 2020; DOI: https://doi.org/10.1007/s12275-020-9630-5

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Abstract

The saprophytic fungus Trichoderma reesei has long been used as a model to study microbial degradation of lignocellulosic biomass. The major cellulolytic enzymes of T. reesei are the cellobiohydrolases CBH1 and CBH2, which constitute more than 70% of total proteins secreted by the fungus. However, their physiological functions and effects on enzymatic hydrolysis of cellulose substrates are not sufficiently elucidated. Here, the cellobiohydrolase-encoding genes cbh1 and cbh2 were deleted, individually or combinatively, by using an auxotrophic marker-recycling technique in T. reesei. When cultured on media with different soluble carbon sources, all three deletion strains (Δcbh1, Δcbh2, and Δcbh1Δcbh2) exhibited no dramatic variation in morphological phenotypes, but their growth rates increased apparently when cultured on soluble cellulase-inducing carbon sources. In addition, Δcbh1 showed dramatically reduced growth and Δcbh1Δcbh2 could hardly grew on microcrystalline cellulose (MCC), whereas all strains grew equally on sodium carboxymethyl cellulose (CMC-Na), suggesting that the influence of the CBHs on growth was carbon source-dependent. Moreover, five representative cellulose substrates were used to analyse the influence of the absence of CBHs on saccharification efficiency. CBH1 deficiency significantly affected the enzymatic hydrolysis rates of various cellulose substrates, where acid pre-treated corn stover (PCS) was influenced the least. CBH2 deficiency reduced the hydrolysis of MCC, PCS, and acid pre-treated and delignified corncob but improved the hydrolysis ability of filter paper. These results demonstrate the specific contributions of CBHs to the hydrolysis of different types of biomass, which could facilitate the development of tailor-made strains with highly efficient hydrolysis enzymes for certain biomass types in the biofuel industry.

Citations

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An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production
Jiaxin Zhang, Kehang Li, Yu Sun, Cheng Yao, Weifeng Liu, Hong Liu, Yaohua Zhong
Biotechnology for Biofuels and Bioproducts.2024;[Epub] CrossRef
Transcriptome-wide analysis of a superior xylan degrading isolate Penicillium oxalicum 5–18 revealed active lignocellulosic degrading genes
Shuang Hu, Pei Han, Bao-Teng Wang, Long Jin, Hong-Hua Ruan, Feng-Jie Jin
Archives of Microbiology.2024;[Epub] CrossRef
Engineering the secretome of Aspergillus niger for cellooligosaccharides production from plant biomass
Fernanda Lopes de Figueiredo, Fabiano Jares Contesini, César Rafael Fanchini Terrasan, Jaqueline Aline Gerhardt, Ana Beatriz Corrêa, Everton Paschoal Antoniel, Natália Sayuri Wassano, Lucas Levassor, Sarita Cândida Rabelo, Telma Teixeira Franco, Uffe Hasb
Microbial Cell Factories.2024;[Epub] CrossRef
Constitutive overexpression of cellobiohydrolase 2 in Trichoderma reesei reveals its ability to initiate cellulose degradation
Yubo Wang, Meibin Ren, Yifan Wang, Lu Wang, Hong Liu, Mei Shi, Yaohua Zhong
Engineering Microbiology.2023; 3(1): 100059. CrossRef
Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
Toshiharu Arai, Mayumi Wada, Hiroki Nishiguchi, Yasushi Takimura, Jun Ishii
Microbial Cell Factories.2023;[Epub] CrossRef
The Influence of Trctf1 Gene Knockout by CRISPR–Cas9 on Cellulase Synthesis by Trichoderma reesei with Various Soluble Inducers
Yudian Chen, Yushan Gao, Zancheng Wang, Nian Peng, Xiaoqin Ran, Tingting Chen, Lulu Liu, Yonghao Li
Fermentation.2023; 9(8): 746. CrossRef
The effect of cellobiohydrolase 1 gene knockout for composition and hydrolytic activity of the enzyme complex secreted by filamentous fungus Penicillium verruculosum
Valeriy Yu. Kislitsin, Andrey M. Chulkin, Ivan N. Zorov, Yuri А. Denisenko, Arkadiy P. Sinitsyn, Alexandra M. Rozhkova
Bioresource Technology Reports.2022; 18: 101023. CrossRef
Deciphering the efficient cellulose degradation by the thermophilic fungus Myceliophthora thermophila focused on the synergistic action of glycoside hydrolases and lytic polysaccharide monooxygenases
Xing Qin, Jiahuan Zou, Kun Yang, Jinyang Li, Xiaolu Wang, Tao Tu, Yuan Wang, Bin Yao, Huoqing Huang, Huiying Luo
Bioresource Technology.2022; 364: 128027. CrossRef

Superantigen SpeA attenuates the biofilm forming capacity of Streptococcus pyogenes: Anshu Babbar , Israel Barrantes , Dietmar H. Pieper , Andreas Itzek; J. Microbiol. 2019;57(7):626-636. Published online June 27, 2019; DOI: https://doi.org/10.1007/s12275-019-8648-z

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Abstract

Beta haemolytic Group A streptococcus (GAS) or Streptococcus pyogenes are strict human pathogens responsible for mild to severe fatal invasive infections. Even with enormous number of reports exploring the role of S. pyogenes exotoxins in its pathogenesis, inadequate knowledge on the biofilm process and the potential role of exotoxins in bacterial dissemination from matured biofilms has been a hindrance in development of effective and targeted treatments. Therefore, the present study was aimed in investigating the uncharted role of these exotoxins in biofilm process. Through our study the putative role of ciaRH in the SpeA dependent ablation of biofilm formation could be speculated and thus helping in bacterial dissemination. The seed-dispersal effect of SpeA was time and concentration dependent and seen to be consistent within various streptococcal species. Transcriptome analysis of SpeA treated S. pyogenes biofilms revealed the involvement of many transcriptional regulators (ciaRH) and response genes (luxS, shr, shp, SPy_0572), hinting towards specific mechanisms underlying the dispersal effect by SpeA. This finding opens up a discussion towards understanding a new mechanism involved in the pathogenesis of Streptococcus pyogenes and might help in understanding the bacterial infections in a better way.

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Pathomolecular epidemiology, antimicrobial resistance, and virulence genes of Streptococcus dysgalactiae subsp. equisimilis isolates from slaughtered pigs in India
Sagar M Patel, Monalisa Sahoo, Jigarji Chaturji Thakor, Dinesh Murali, Pradeep Kumar, Rajendra Singh, Karam Pal Singh, G Saikumar, Chandrakanta Jana, Shailesh Kumar Patel, Akash B Mote, Ravichandran Karthikeyan, Rajesh Kumar Vandre, Jitendra Kumar Biswal,
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Abstract

The purpose of this study was to analyze and compare genes encoding superantigens (SAgs) in Staphylococcus xylosus and Staphylococcus aureus isolates collected simultaneously from milk of the same cows with clinical mastitis. Genes encoding staphylococcal enterotoxins and enterotoxin-like proteins (sea-selu), toxic shock syndrome toxin 1 (tst-1) and exfolia-tive toxins (eta and etd) were investigated. It was found that among 30 isolates of S. xylosus, 16 (53.3%) harbored from 1 to 10 SAg genes. In total, in 16 SAg positive S. xylosus, 11 different enterotoxin genes were detected: sec, sed, seg, seh, sei, selm, seln, selo, selp, ser, selu and one etd gene encoding exfoliative toxin D. The most prevalent genes were ser, selu, and selo. Among all the positive isolates of S. xylosus, a total of 14 different SAg gene combinations were detected. One combination was repeated in 3 isolates, whereas the rest were detected only once. However, in the case of S. aureus all the 30 isolates harbored the same combination of SAg genes: seg, sei, selm, seln, selo and on the basis of PFGE analysis all belonged to the same clonal type. Also noteworthy was the observation that SAg genes detected in S. aureus have also been found in S. xylosus. The findings of this study further extend previous observations that SAg genes are present not only in S. aureus but also in coagulase-negative staphy-lococci, including S. xylosus. Therefore, taking into account that the SAg genes are encoded on mobile genetic elements it is possible that these genes can be transferred between different species of coexisting staphylococci.

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