Journal of Microbiology

Journal Article

Effect of biostimulation and bioaugmentation on hydrocarbon degradation and detoxification of diesel-contaminated soil: a microcosm study: Patricia Giovanella , Lídia de Azevedo Duarte , Daniela Mayumi Kita , Valéria Maia de Oliveira , Lara Durães Sette; J. Microbiol. 2021;59(7):634-643. Published online May 15, 2021; DOI: https://doi.org/10.1007/s12275-021-0395-2

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Soil contamination with diesel oil is quite common during processes of transport and storage. Bioremediation is considered a safe, economical, and environmentally friendly approach for contaminated soil treatment. In this context, studies using hydrocarbon bioremediation have focused on total petroleum hydrocarbon (TPH) analysis to assess process effectiveness, while ecotoxicity has been neglected. Thus, this study aimed to select a microbial consortium capable of detoxifying diesel oil and apply this consortium to the bioremediation of soil contaminated with this environmental pollutant through different bioremediation approaches. Gas chromatography (GC-FID) was used to analyze diesel oil degradation, while ecotoxicological bioassays with the bioindicators Artemia sp., Aliivibrio fischeri (Microtox), and Cucumis sativus were used to assess detoxification. After 90 days of bioremediation, we found that the biostimulation and biostimulation/ bioaugmentation approaches showed higher rates of diesel oil degradation in relation to natural attenuation (41.9 and 26.7%, respectively). Phytotoxicity increased in the biostimulation and biostimulation/bioaugmentation treatments during the degradation process, whereas in the Microtox test, the toxicity was the same in these treatments as that in the natural attenuation treatment. In both the phytotoxicity and Microtox tests, bioaugmentation treatment showed lower toxicity. However, compared with natural attenuation, this approach did not show satisfactory hydrocarbon degradation. Based on the microcosm experiments results, we conclude that a broader analysis of the success of bioremediation requires the performance of toxicity bioassays.

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Environmental Science and Pollution Research.2024; 31(20): 30026. CrossRef
Soil Corrosivity Under Natural Attenuation
Larissa O. da Silva, Sara H. de Oliveira, Rafael G. C. da Silva, Magda R. S. Vieira, Ivanilda R. de Melo, Severino L. Urtiga Filho
Materials Research.2024;[Epub] CrossRef
Updating risk remediation-endpoints for petroleum-contaminated soils? A case study in the Ecuadorian Amazon region
Daniel Hidalgo-Lasso, Karina García-Villacís, Jeaneth Urvina Ulloa, Darwin Marín Tapia, Patricio Gómez Ortega, Frederic Coulon
Heliyon.2024; 10(9): e30395. CrossRef
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Yingying Li, Yuankun Zhao, Yiyang Du, Xuechun Ren, He Ding, Zhimin Wang
Luminescence.2024;[Epub] CrossRef
Oil biodegradation studies with an immobilized bacterial consortium in plant biomass for the construction of bench-scale bioreactor
Rachel M. Ferreira, Bernardo D. Ribeiro, Danielle.M.A. Stapelfeldt, Rodrigo P. do Nascimento, Maria de.F.R. Moreira
Cleaner Chemical Engineering.2023; 6: 100107. CrossRef
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Kai Zhang, Meng Liu, Xinlong Song, Dongyu Wang
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Research Support, Non-U.S. Gov'ts

NOTE] Identification of Secreted Virulence Factors of Chromobacterium violaceum: Thiago Castro-Gomes , Mariana S. Cardoso , Wanderson D. DaRocha , Letícia A. Laibida , Andréa M. A. Nascimento , Luciana W. Zuccherato , Maria Fátima Horta , Marcelo P. Bemquerer , Santuza M. R. Teixeira; J. Microbiol. 2014;52(4):350-353. Published online February 17, 2014; DOI: https://doi.org/10.1007/s12275-014-3202-5

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Abstract

Chromobacterium violaceum, a component of tropical soil microbiota, is an opportunistic pathogenic bacterium that can infect humans and other animals. In addition to identifying a large number of genes that demonstrate the vast biotechnological potential of this bacterium, genome sequencing revealed several virulence factors, including different cytolysins, which can be related to its pathogenicity. Here we confirmed these predictions from genomic analyses by identifying, through mass spectrometry, proteins present in the culture supernatant of C. violaceum that may constitute secreted virulence factors. Among them, we identified a secreted collagenase and the product of a gene with sequence similarity to previously characterized bacterial porins.

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Kai-Zhong Xu, Xiao-Juan Tan, Zeng-Yan Chang, Jun-Jian Li, Ai-Qun Jia
LWT.2022; 163: 113569. CrossRef
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Somdeb BoseDasgupta, Jean Pieters
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Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472
Rafael A. Baraúna, Agenor V. Santos, Diego A. Graças, Daniel M. Santos, Rubens Ghilardi Júnior, Adriano M. C. Pimenta, Marta S. P. Carepo, Maria P.C. Schneider, Artur Silva
Genetics and Molecular Biology.2015; 38(2): 227. CrossRef
Guava Leaf Extract Inhibits Quorum-Sensing and Chromobacterium violaceum Induced Lysis of Human Hepatoma Cells: Whole Transcriptome Analysis Reveals Differential Gene Expression
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PLoS ONE.2014; 9(9): e107703. CrossRef

Swarming Differentiation of Vibrio vulnificus Downregulates the Expression of the vvhBA Hemolysin Gene via the LuxS Quorum-Sensing System: Moon-Young Kim , Ra-Young Park , Mi-Hwa Choi , Hui-Yu Sun , Choon-Mee Kim , Soo-Young Kim , Joon-Haeng Rhee , Sung-Heui Shin; J. Microbiol. 2006;44(2):226-232.; DOI: https://doi.org/2361 [pii]

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Abstract: Swarming has proven to be a good in vitro model for bacterial surface adherence and colonization, and the swarming differentiation of a bacterium has been shown to be coupled with changes in the expression of virulence factors associated with its invasiveness, particularly in the early stages of infection. In this study, we attempted to determine whether the expression of vvhA, which encodes for hemolysin/cytolysin (VvhA), is either upregulated or downregulated during the swarming differentiation of V. vulnificus. The insertional inactivation of vvhA itself exerted no detectable effect on the expression of V. vulnificus swarming motility. However, in our lacZ-fused vvhA transcriptional reporter assay, vvhA expression decreased in swarming V. vulnificus as compared to non-swarming or planktonic V. vulnificus. The reduced expression of vvhA in swarming V. vulnificus increased as a result of the deletional inactivation of luxS, a gene associated with quorum sensing. These results show that vvhA expression in swarming V. vulnificus is downregulated via the activity of the LuxS quorum-sensing system, suggesting that VvhA performs no essential role in the invasiveness of V. vulnificus via the adherence to and colonization on the body surfaces required in the early stages of the infection. However, VvhA may play a significant role in the pathophysiological deterioration occurring after swarming V. vulnificus is differentiated into planktonic V. vulnificus.

Journal Article

The Viable But Nonculturable State of Kanagawa Positive and Negative Strains of Vibrio parahaemolyticus: Tonya C. Bates , James D. Oliver; J. Microbiol. 2004;42(2):74-79.; DOI: https://doi.org/2042 [pii]

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Abstract: Ingestion of shellfish-associated Vibrio parahaemolyticus is the primary cause of potentially severe gastroenteritis in many countries. However, only Kanagawa phenomenon (hemolysin) positive (KP^+) strains of V. parahaemolyticus are isolated from patients, whereas >99% of strains isolated from the environment do not produce this hemolysin (i.e. are KP^-). The reasons for these differences are not known. Following a temperature downshift, Vibrio parahaemolyticus enters the viable but nonculturable (VBNC) state wherein cells maintain viability but cannot be cultured on routine microbiological media. We speculated that KP^+ and KP^- strains may respond differently to the temperature and salinity conditions of seawater by entering into this state which might account for the low numbers of culturable KP^+ strains isolated from estuarine waters. The response of eleven KP^+ and KP^- strains of V. parahaemolyticus following exposure to a nutrient and temperature downshift in different salinities, similar to conditions encountered in their environment, was examined. The strains included those from which the KP^+ genes had been selectively removed or added. Our results indicated that the ability to produce hemolysin did not affect entrance into the VBNC state. Further, VBNC cells of both biotypes could be restored to the culturable state following an overnight temperature upshift.

Identification of hemolysin as one of the important virulent factors in vibrio anguillarum V7: Choe, Young Chool , Jeong, Ga Jin; J. Microbiol. 1995;33(4):283-288.

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Abstract: We have identified hemolysin rendering virulency of Vibrio anguilarum grown at 23℃ which was evaluated on human RBCs. Hemolysin itself was separated as a single band on non-denaturing gel electrophoresis. Vibrio hemolysin was destroyed by trypsin and proteinase K and was heat labile. Optimal pH for activity was around pH 6 while pI of the molecule was recognized as 5.7, with relative distance (R_f) on non-denaturing gel was 0.7. Addition of EDTA and FeCI₃drew the possibility that the production of hemolysin was mainly induced to overcome iron deficiency inside host animals upon infection.

Physiological characterization of kinetics and action mechanism of vibrio hemolysin: Choe, Young Chool , Jeong, Ga Jin; J. Microbiol. 1995;33(4):289-294.

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Abstract: The action mechanism of hemolysin rendering virulency of Vibrio anguilarum has not clarified as yet, even though there were several possible factors explained. We have studied hemolytic kinetics performed by hemolysin from V. anguillarum strain V7 as well as binding of hemolysin to RBC membrane. Maximal rate of hemolysis and duration of lag phase were directly and inversly correlated to the concentration of hemolysin used. Hemolysin molecules are known to bind consumptively with proper diameter, while other protectants with smaller diameter could not. In conclusion, hemolysin should bind irreversibly to RBC membrane exert hemolysis distorting osmotic pressure. The binding could be hindered by spatial structure of the RBC surfacem which might be caused by sialic acid.

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