Journal of Microbiology

Journal Articles

The C-22 sterol desaturase Erg5 is responsible for ergosterol biosynthesis and conidiation in Aspergillus fumigatus: Nanbiao Long , Guowei Zhong; J. Microbiol. 2022;60(6):620-626. Published online April 18, 2022; DOI: https://doi.org/10.1007/s12275-022-1564-7

59 View
0 Download
4 Web of Science
4 Crossref

Aspergillus fumigatus is the most prevalent saprophytic fungi and can cause severe invasive aspergillosis in immunocompromised individuals. For infection of A. fumigatus, the small hydrophobic conidia have been shown to play a dominant role. In this study, we found that deletion of erg5, a C-22 sterol desaturase gene which function in the last two steps of ergosterol biosynthesis, was sufficient to block ergosterol biosynthesis and conidiation. The deletion phenotype was further verified by a conditional expression strain of erg5 using the inducible tet-on system. Strikingly, erg5 mutant displays increased susceptibility to antifungal azoles itraconazole. RNA sequencing analysis showed that erg5 deficiency resulted in changes in transcription mainly related to lipid, carbohydrate, and amino acid metabolism. Genes encoding ergosterol biosynthesis- related enzymes were found to be up-regulated in erg5 null mutants. However, genes involved in asexual development, including upstream regulators, melanin biosynthesis enzymes, heterotrimeric G proteins, and MAPK signaling, were down-regulated to various degrees. Furthermore, metabolomic study revealed that erg5 deficiency also resulted in altered lipid and amino acid metabolism, which was consistent with our transcriptomics analysis. Collectively, our study established a link between ergosterol biosynthesis and asexual development at the transcriptomics and metabolomics level in A. fumigatus.

Citations

Citations to this article as recorded by

Quantitative proteomic analysis reveals Ga(III) polypyridyl catecholate complexes disrupt Aspergillus fumigatus mitochondrial function
Magdalena Piatek, Brunella Grassiri, Lewis More O’Ferrall, Anna Maria Piras, Giovanna Batoni, Semih Esin, Christine O’Connor, Darren Griffith, Anne Marie Healy, Kevin Kavanagh
JBIC Journal of Biological Inorganic Chemistry.2024; 29(7-8): 707. CrossRef
Ergosterol Is Critical for Sporogenesis in Cryptococcus neoformans
Amber R. Matha, Xiaofeng Xie, Xiaorong Lin
Journal of Fungi.2024; 10(2): 106. CrossRef
Erg4 Is Involved in Ergosterol Biosynthesis, Conidiation and Stress Response in Penicillium expansum
Zhanhong Han, Yuanyuan Zong, Xuemei Zhang, Di Gong, Bin Wang, Dov Prusky, Edward Sionov, Huali Xue, Yang Bi
Journal of Fungi.2023; 9(5): 568. CrossRef
A chromosome-scale genome assembly of the grape powdery mildew pathogen Erysiphe necator reveals its genomic architecture and previously unknown features of its biology
Alex Z. Zaccaron, Tara Neill, Jacob Corcoran, Walter F. Mahaffee, Ioannis Stergiopoulos, Gustavo H. Goldman
mBio.2023;[Epub] CrossRef

Assessment of Cre-lox and CRISPR-Cas9 as tools for recycling of multiple-integrated selection markers in Saccharomyces cerevisiae: Hye Yun Moon† , Gyu Hun Sim† , Hyeon Jin Kim , Keunpil Kim , Hyun Ah Kang; J. Microbiol. 2022;60(1):18-30. Published online December 29, 2021; DOI: https://doi.org/10.1007/s12275-022-1580-7

58 View
0 Download
7 Web of Science
7 Crossref

Abstract

We evaluated the Cre-lox and CRISPR-Cas9 systems as markerrecycling tools in Saccharomyces cerevisiae recombinants containing multiple-integrated expression cassettes. As an initial trial, we constructed rDNA-nontranscribed spacer- or Ty4- based multiple integration vectors containing the URA3 marker flanked by the loxP sequence. Integrants harboring multiple copies of tHMG1 and NNV-CP expression cassettes were obtained and subsequently transformed with the Cre plasmid. However, the simultaneous pop-out of the expression cassettes along with the URA3 marker hampered the use of Cre-lox as a marker-recycling tool in multiple integrants. As an alternative, we constructed a set of CRISPR-Cas9-gRNA vectors containing gRNA targeted to auxotrophic marker genes. Transformation of multiple integrants of tHMG1 and NNV-CP cassettes by the Cas9-gRNA vector in the presence of the URA3 (stop) donor DNA fragments generated the Ura- transformants retaining multiple copies of the expression cassettes. CRISPR-Cas9-based inactivation led to the recycling of the other markers, HIS3, LEU2, and TRP1, without loss of expression cassettes in the recombinants containing multiple copies of tHMG1, NNV-CP, and SfBGL1 cassettes, respectively. Reuse of the same selection marker in marker-inactivated S. cerevisiae was validated by multiple integrations of the TrEGL2 cassette into the S. cerevisiae strain expressing SfBGL1. These results demonstrate that introducing stop codons into selection marker genes using the CRISPR-Cas9 system with donor DNA fragments is an efficient strategy for markerrecycling in multiple integrants. In particular, the continual reuse of auxotrophic markers would facilitate the construction of a yeast cell factory containing multiple copies of expression cassettes without antibiotic resistance genes.

Citations

Citations to this article as recorded by

Multiple metabolic engineering of Saccharomyces cerevisiae for the production of lycopene
Jiaheng Liu, Minxia Song, Xianhao Xu, Yaokang Wu, Yanfeng Liu, Guocheng Du, Jianghua Li, Long Liu, Xueqin Lv
Food Bioengineering.2024;[Epub] CrossRef
Biochemical and Biorefinery Platform for Second-Generation Bioethanol: Fermentative Strategies and Microorganisms
Karla D. González-Gloria, Elia Tomás-Pejó, Lorena Amaya-Delgado, Rosa M. Rodríguez-Jasso, Araceli Loredo-Treviño, Anusuiya Singh, Meenu Hans, Carlos Martín, Sachin Kumar, Héctor A. Ruiz
Fermentation.2024; 10(7): 361. CrossRef
CRISPR/Cas9-based toolkit for rapid marker recycling and combinatorial libraries in Komagataella phaffii
Wei Zhou, Yuanyi Li, Guosong Liu, Weichuang Qin, Dongzhi Wei, Fengqing Wang, Bei Gao
Applied Microbiology and Biotechnology.2024;[Epub] CrossRef
Establishment, optimization, and application of genetic technology in Aspergillus spp.
Jing Gao, Huiqing Liu, Zhenzhen Zhang, Zhihong Liang
Frontiers in Microbiology.2023;[Epub] CrossRef
CRISPR-Cas Technology for Bioengineering Conventional and Non-Conventional Yeasts: Progress and New Challenges
Yuanyuan Xia, Yujie Li, Wei Shen, Haiquan Yang, Xianzhong Chen
International Journal of Molecular Sciences.2023; 24(20): 15310. CrossRef
Genomic and functional features of yeast species in Korean traditional fermented alcoholic beverage and soybean products
Da Min Jeong, Hyeon Jin Kim, Min-Seung Jeon, Su Jin Yoo, Hye Yun Moon, Eun-joo Jeon, Che Ok Jeon, Seong-il Eyun, Hyun Ah Kang
FEMS Yeast Research.2023;[Epub] CrossRef
Multiplex genome editing to construct cellulase engineered Saccharomyces cerevisiae for ethanol production from cellulosic biomass
Yatika Dixit, Preeti Yadav, Arun Kumar Sharma, Poornima Pandey, Arindam Kuila
Renewable and Sustainable Energy Reviews.2023; 187: 113772. CrossRef

Promising cellulolytic fungi isolates for rice straw degradation: Diana Catalina Pedraza-Zapata , Andrea Melissa Sánchez-Garibello , Balkys Quevedo-Hidalgo , Nubia Moreno-Sarmiento , Ivonne Gutiérrez-Rojas; J. Microbiol. 2017;55(9):711-719. Published online September 2, 2017; DOI: https://doi.org/10.1007/s12275-017-6282-1

46 View
0 Download
17 Crossref

Abstract

The objective of this study was to evaluate the potential of eight fungal isolates obtained from soils in rice crops for straw degradation in situ. From the initial eight isolates, Pleurotus ostreatus T1.1 and Penicillium sp. HC1 were selected for further characterization based on qualitative cellulolytic enzyme production and capacity to use rice straw as a sole carbon source. Subsequently, cellulolytic, xylanolytic, and lignolytic (Pleurotus ostreatus) activity on carboxymethyl cellulose, oat xylan, and rice straw with different nitrogen sources was evaluated. From the results obtained it was concluded both isolates are capable to produce enzymes necessary for rice straw degradation. However, their production is dependent upon carbon and nitrogen source. Last, it was established that Pleurotus ostreatus T1.1 and Penicillium sp. HC1 capability to colonize and mineralize rice straw, in mono-and co-culture, without affecting nitrogen soil content.

Citations

Citations to this article as recorded by

Unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradation
H.K. Narendra Kumar, N. Chandra Mohana, Jayarama Reddy, M.R. Abhilash, S. Satish
The Microbe.2024; 5: 100183. CrossRef
Sustainability in residue management: a review with special reference to Indian agriculture
Meenakshi Verma, Pooja Singh, Manikprabhu Dhanorkar
Paddy and Water Environment.2024; 22(1): 1. CrossRef
Crop residue heterogeneity: Decomposition by potential indigenous ligno-cellulolytic microbes and enzymatic profiling
Sandeep Sharma, Kailash Chand Kumawat, Paawan Kaur, Sukhjinder Kaur, Nihar Gupta
Current Research in Microbial Sciences.2024; 6: 100227. CrossRef
Recent developments in microbial degradation of crop residues: a comprehensive review
K. S. Sruthy, S. Puranik, V. Kumar, A. Kaushik, K. V. Vikram, M. Manoj, L. Shukla, S. K. Singh, A. Kumar
International Journal of Environmental Science and Technology.2024;[Epub] CrossRef
Fungal Saprotrophic Promotion and Plant Pathogenic Suppression under Ditch-Buried Straw Return with Appropriate Burial Amount and Depth
Jie Zhou, Yanling Li, Jiawen Lou, Yuekai Wang, Zhengrong Kan, Reinhard W. Neugschwandtner, Fengmin Li, Jian Liu, Ke Dong, Yaguang Xue, Haishui Yang, Lingling Shi
Plants.2024; 13(13): 1738. CrossRef
Rice crop residue management by the microbial consortium for rapid decomposition of straw
Kunvar Gyanendra Kumar, Raja Husain, Anurag Mishra, Nitin Vikram, Devendra Kumar Dwivedi, Saurabh Pandey, Ashutosh Singh
3 Biotech.2024;[Epub] CrossRef
Highly variable domain architecture in carbohydrate-active enzymes highlights Streptomyces as promising resource for rice straw bioconversion
Andika Sidar, Gerben P. Voshol, Ahmed El-Masoudi, Erik Vijgenboom, Peter J. Punt
Bioresource Technology Reports.2024; 25: 101775. CrossRef
Isolation and Characterization of Cellulolytic Fungi From Decomposing Rice Straws
Shir Nee Ong, Chin Mei Lee
Malaysian Applied Biology.2023; 52(4): 1. CrossRef
Biodegradation of Gossypol by Aspergillus terreus-YJ01
Yao Jiang, Xinyue Du, Qianqian Xu, Chunhua Yin, Haiyang Zhang, Yang Liu, Xiaolu Liu, Hai Yan
Microorganisms.2023; 11(9): 2148. CrossRef
Tomato plant extract (Lycopersicon esculentum) obtained from agroindustrial byproducts and its antifungal activity against Fusarium spp.
Luis M. Isidro-Requejo, Enrique Márquez-Ríos, Carmen L. Del Toro-Sánchez, Saúl Ruiz-Cruz, Daniel Valero-Garrido, Guadalupe M. Suárez-Jiménez
Frontiers in Sustainable Food Systems.2023;[Epub] CrossRef
Mixed Cultures of Aspergillus niger and Rhizopus oryzae Using Lignocellulosic Substrates to Improve Hydrolytic Enzyme Production
Esteban Amador Morilla, Antonela Taddia, Maximiliano Sortino, Gisela Tubio
BioEnergy Research.2023; 16(4): 2285. CrossRef
Identification of volatile components and growth promoting effects of endophytes of Tinospora capillipes Gagnep.
Qianxi Li, Siyu Wang, Senmiao Chen, Yini Shang, Fangmei Zhou, Jie Yu, Zhishan Ding, Xiaoqing Ye
Journal of Plant Pathology.2022; 105(1): 57. CrossRef
Degradation of lignocelluloses in straw using AC-1, a thermophilic composite microbial system
Hongdou Liu, Liqiang Zhang, Yu Sun, Guangbo Xu, Weidong Wang, Renzhe Piao, Zongjun Cui, Hongyan Zhao
PeerJ.2021; 9: e12364. CrossRef
Wheat straw hydrolysis by using co-cultures of Trichoderma reesei and Monascus purpureus toward enhanced biodegradation of the lignocellulosic biomass in bioethanol biorefinery
Shabih Fatma, Aimen Saleem, Romana Tabassum
Biomass Conversion and Biorefinery.2021; 11(3): 743. CrossRef
WITHDRAWN: Paddy straw procurement challenges and its effect on cost of power generation
Manjeet Singh, Yadwinder Singh Brar, Harpuneet Singh
Materials Today: Proceedings.2021;[Epub] CrossRef
Control biológico: Camino a la agricultura moderna
Diana Marcela Vinchira-Villarraga, Nubia Moreno-Sarmiento
Revista Colombiana de Biotecnología.2019; 21(1): 2. CrossRef
Characterization of an Anaerobic, Thermophilic, Alkaliphilic, High Lignocellulosic Biomass-Degrading Bacterial Community, ISHI-3, Isolated from Biocompost
Ayumi Shikata, Junjarus Sermsathanaswadi, Phakhinee Thianheng, Sirilak Baramee, Chakrit Tachaapaikoon, Rattiya Waeonukul, Patthra Pason, Khanok Ratanakhanokchai, Akihiko Kosugi
Enzyme and Microbial Technology.2018; 118: 66. CrossRef

Research Support, Non-U.S. Gov'ts

New record and enzyme activity of four species in Penicillium section Citrina from marine environments in Korea: Myung Soo Park , Ji Eun Eom , Jonathan J. Fong , Young Woon Lim; J. Microbiol. 2015;53(4):219-225. Published online April 8, 2015; DOI: https://doi.org/10.1007/s12275-015-4700-9

46 View
0 Download
14 Crossref

Abstract

Several strains of Penicillium section Citrina were isolated during a survey of fungi from marine environments along the southern coast of Korea. Based on multigene phylogenetic analyses (?tubulin and calmodulin) and morphological characteristics, the 11 strains were identified as P. citrinum, P. hetheringtonii, P. paxilli, P. sumatrense, P. terrigenum, and P. westlingii. To understand the ecological role of these species, we tested all strains for extracellular enzyme activity; six strains representing four species showed ?glucosidase activity. Four of the identified species ?P. hetheringtonii, P. paxilli, P. terrigenum, and P. westlingii ?are new records for Korea. For these new species records, we describe morphological characteristics of the strains and compare results to published data of type strains.

Citations

Citations to this article as recorded by

Antiproliferative Activity Compounds Isolated from the Marine Fungus Penicillium terrigenum
Fan-ying Wu, Si-yuan Liu, Jie Feng
Pharmaceutical Chemistry Journal.2024; 58(5): 759. CrossRef
New Bioactive β-Resorcylic Acid Derivatives from the Alga-Derived Fungus Penicillium antarcticum KMM 4685
Elena V. Leshchenko, Alexandr S. Antonov, Gleb V. Borkunov, Jessica Hauschild, Olesya I. Zhuravleva, Yuliya V. Khudyakova, Alexander S. Menshov, Roman S. Popov, Natalya Yu Kim, Markus Graefen, Carsten Bokemeyer, Gunhild von Amsberg, Anton N. Yurchenko, Se
Marine Drugs.2023; 21(3): 178. CrossRef
Species Diversity of Penicillium in Southwest China with Discovery of Forty-Three New Species
Xin-Cun Wang, Zhi-Kang Zhang, Wen-Ying Zhuang
Journal of Fungi.2023; 9(12): 1150. CrossRef
Diversity and Dynamics of Marine Arenicolous Fungi in Three Seasides of the Korean Peninsula
Jun Won Lee, Chang Wan Seo, Wonjun Lee, Ji Seon Kim, Ki Hyeong Park, Yoonhee Cho, Young Woon Lim
Journal of Microbiology.2023; 61(1): 63. CrossRef
Titanium-based photocatalytic coatings for bacterial disinfection: The shift from suspended powders to catalytic interfaces
Farnaz Hosseini, Aymen Amine Assadi, Phuong Nguyen-Tri, Imran Ali, Sami Rtimi
Surfaces and Interfaces.2022; 32: 102078. CrossRef
Plastic-inhabiting fungi in marine environments and PCL degradation activity
Sung Hyun Kim, Jun Won Lee, Ji Seon Kim, Wonjun Lee, Myung Soo Park, Young Woon Lim
Antonie van Leeuwenhoek.2022; 115(12): 1379. CrossRef
Penicillium from Rhizosphere Soil in Terrestrial and Coastal Environments in South Korea
Myung Soo Park, Jun Won Lee, Sung Hyun Kim, Ji-Hyun Park, Young-Hyun You, Young Woon Lim
Mycobiology.2020; 48(6): 431. CrossRef
Biodiversity of Penicillium species from marine environments in Portugal and description of Penicillium lusitanum sp. nov., a novel species isolated from sea water
Micael F. M. Gonçalves, Liliana Santos, Bruno M. V. Silva, Alberto C. Abreu, Tânia F. L. Vicente, Ana C. Esteves, Artur Alves
International Journal of Systematic and Evolutionary Microbiology.2019; 69(10): 3014. CrossRef
Fungal Diversity and Enzyme Activity Associated with the Macroalgae, Agarum clathratum
Seobihn Lee, Myung Soo Park, Hanbyul Lee, Jae-Jin Kim, John A. Eimes, Young Woon Lim
Mycobiology.2019; 47(1): 50. CrossRef
New Records of Four Species Belonging to Eurotiales from Soil and Freshwater in Korea
Monmi Pangging, Thuong T. T. Nguyen, Hyang Burm Lee
Mycobiology.2019; 47(2): 154. CrossRef
Three Unrecorded Species Belonging toPenicilliumSectionSclerotiorafrom Marine Environments in Korea
Myung Soo Park, Dawoon Chung, Kyunghwa Baek, Young Woon Lim
Mycobiology.2019; 47(2): 165. CrossRef
A New record of four Penicillium species isolated from Agarum clathratum in Korea
Myung Soo Park, Seobihn Lee, Young Woon Lim
Journal of Microbiology.2017; 55(4): 237. CrossRef
Diversity and enzyme activity of Penicillium species associated with macroalgae in Jeju Island
Myung Soo Park, Seobihn Lee, Seung-Yoon Oh, Ga Youn Cho, Young Woon Lim
Journal of Microbiology.2016; 54(10): 646. CrossRef
Castles fall from inside: Evidence for dominant internal photo-catalytic mechanisms during treatment of Saccharomyces cerevisiae by photo-Fenton at near-neutral pH
Stefanos Giannakis, Cristina Ruales-Lonfat, Sami Rtimi, Sana Thabet, Pascale Cotton, César Pulgarin
Applied Catalysis B: Environmental.2016; 185: 150. CrossRef

Transformation of Inorganic P Fractions of Soil and Plant Growth Promotion by Phosphate-solubilizing Ability of Penicillium oxalicum I1: Mingbo Gong , Peng Du , Xue Liu , Changxiong Zhu; J. Microbiol. 2014;52(12):1012-1019. Published online November 3, 2014; DOI: https://doi.org/10.1007/s12275-014-4406-4

45 View
0 Download
10 Crossref

Abstract

The solubilization of tricalcium phosphate is often considered as the standard for screening of most phosphate-solubilizing microorganisms (PSMs). However, usually the effect of large-scale application of PSM on the promotion of crop growth varies. This study presents an efficient method for screening and testing phosphate-solubilizing fungus that enhance plant growth. A fungus Penicillium oxalicum I1 (PI1) was isolated and identified that had high ability of phosphate- solubilization and could utilize maize root exudates as sources, and propagate well in vitro and in soil. P-I1 excreted oxalic acid and reached 593.9 μg/ml, and the pH value was decreased from 6.90 to 1.65 in 26 h. The amount of P-I1 increased by 48-fold in 28 d and was maintained for 49 d in soil. PSM showed selectivity on the transformation of the different forms of phosphorus, a wide range of insoluble phosphates, such as Ca8H2(PO4)6·5H2O, AlPO4, FePO4, and Ca10(PO4)6(OH)2, were converted to soluble CaHPO4 in soil, and CaHPO4 was also inhibited from being converted into insoluble phosphate by P-I1. The Ca2-P content reached 27.11 μg/g soil on day 28 at 20°C, which increased by 110.32%, and plant growth promotion was tested and verified, the
results
showed that maize yield increased remarkably than control after inoculated P-I1, maize yield increased maximum by 14.47%. The data presented that P-I1 appear attractive for exploring their plant growth-promoting activity and potential field application.

Citations

Citations to this article as recorded by

Inoculations of phosphate-solubilizing bacteria alter soil microbial community and improve phosphorus bioavailability for moso bamboo (Phyllostachys edulis) growth
Yaohui Liu, Ashrafun Nessa, Qiyuan Zheng, Dongnan Hu, Wenyuan Zhang, Manyun Zhang
Applied Soil Ecology.2023; 189: 104911. CrossRef
Biological Control Potential of Endophytic Fungi with Amelioration of Systemic Resistance in Sunflower and GC–MS Metabolic Profiling of Talaromyces assiutensis
Hafiza Farhat, Faizah Urooj, Muhammed Irfan, Nida Sohail, Saima Majeed, Shahid Ullah, Hafza Asma Shafique
Current Microbiology.2023;[Epub] CrossRef
Effect of Bacillus megaterium var. phosphaticum applied together with rock phosphate on wheat yield and some soil properties in a calcareous soil
Betül BAYRAKLI
EURASIAN JOURNAL OF SOIL SCIENCE (EJSS).2022; 11(3): 198. CrossRef
Plant Growth Promoting Filamentous Fungi and Their Application in the Fertilization of Pastures for Animal Consumption
Rosalba Argumedo-Delira, Mario J. Gómez-Martínez, Jairo Mora-Delgado
Agronomy.2022; 12(12): 3033. CrossRef
The fungal and archaeal community within plant rhizosphere: a review on their contribution to crop safety
Saheed Adekunle Akinola, Olubukola Oluranti Babalola
Journal of Plant Nutrition.2021; 44(4): 600. CrossRef
A comprehensive synthesis unveils the mysteries of phosphate‐solubilizing microbes
Jin‐tian Li, Jing‐li Lu, Hong‐yu Wang, Zhou Fang, Xiao‐juan Wang, Shi‐wei Feng, Zhang Wang, Ting Yuan, Sheng‐chang Zhang, Shu‐ning Ou, Xiao‐dan Yang, Zhuo‐hui Wu, Xiang‐deng Du, Ling‐yun Tang, Bin Liao, Wen‐sheng Shu, Pu Jia, Jie‐Liang Liang
Biological Reviews.2021; 96(6): 2771. CrossRef
Effect of Acid Production by Penicillium oxalicum on Physicochemical Properties of Bauxite Residue
Yifan Zhang, Rui Xue, Xuan He, Qingyu Cheng, William Hartley, Shengguo Xue
Geomicrobiology Journal.2020; 37(10): 929. CrossRef
Sustainable Chemistry: Solubilization of Phosphorus from Insoluble Phosphate Material Hydroxyapatite with Ozonized Biochar
Oumar Sacko, Rachel Whiteman, Gyanendra Kharel, Sandeep Kumar, James W. Lee
ACS Sustainable Chemistry & Engineering.2020; 8(18): 7068. CrossRef
Characterization and variation of the rhizosphere fungal community structure of cultivated tetraploid cotton
Qinghua Qiao, Jingxia Zhang, Changle Ma, Furong Wang, Yu Chen, Chuanyun Zhang, Hui Zhang, Jun Zhang, Vijai Gupta
PLOS ONE.2019; 14(10): e0207903. CrossRef
Gibberellins in Penicillium strains: Challenges for endophyte-plant host interactions under salinity stress
Ana Lúcia Leitão, Francisco J. Enguita
Microbiological Research.2016; 183: 8. CrossRef

Novel Mutations in CYP51B from Penicillium digitatum Involved in Prochloraz Resistance: Jinlong Wang , Jinhui Yu , Jing Liu , Yongze Yuan , Na Li , Muqing He , Ting Qi , Geng Hui , Li Xiong , Deli Liu; J. Microbiol. 2014;52(9):762-770. Published online August 2, 2014; DOI: https://doi.org/10.1007/s12275-014-4112-2

47 View
0 Download
46 Crossref

Abstract

Green mold caused by Penicillium digitatum is one of the most serious postharvest diseases of citrus fruit, and it is ubiquitous in all citrus growing regions in the world. Sterol 14α-demethylase (CYP51) is one of the key enzymes of sterol biosynthesis in the biological kingdom and a prime target of antifungal drugs. Mutations in CYP51s have been found to be correlated with resistance to azole fungicides in many fungal species. To investigate the mechanism of resistance to prochloraz (PRC) in P. digitatum, the PRC sensitivity was determined in vitro in this study to assess the sensitivity of 78 P. digitatum isolates collected in Hubei province. The results showed that 25 isolates were prochloraz-resistant (PRC-R), including six high-resistant (HR) strains, twelve medium-resistant (MR) and seven low-resistant (LR) strains. A sequence analysis showed no consistent point mutations of PdCYP51A in the PRC-R strains, but four substitutions of CYP51B were found, Q309H in LR strains, Y136H and Q309H in HR strains, and G459S and F506I in MR strains, which corresponded to the four sensitivity levels. Based on the sequence alignment analysis and homology modeling followed by the molecular docking of the PdCYP51B protein, the potential correlation between the mutations and PRC resistance is proposed.

Citations

Citations to this article as recorded by

Mechanism and effectiveness of natamycin produced by Streptomyces philanthi RL-1-178 in controlling green mold disease caused by Penicillium digitatum on postharvest mandarin fruit (Citrus reticulata Blanco)
Sawai Boukaew, Krittin Chumkaew, Sirasit Srinuanpan
Journal of Agriculture and Food Research.2025; 19: 101585. CrossRef
Transcriptome Analysis of mfs2-Defective Penicillium digitatum Mutant to Reveal Importance of Pdmfs2 in Developing Fungal Prochloraz Resistance
Rongrong Cuan, Shaoting Liu, Chuanyou Zhou, Shengqiang Wang, Yongliang Zheng, Yongze Yuan
Microorganisms.2024; 12(5): 888. CrossRef
Transcriptome Analysis Reveals Potential Regulators of DMI Fungicide Resistance in the Citrus Postharvest Pathogen Penicillium digitatum
Yue Xi, Jing Zhang, Botao Fan, Miaomiao Sun, Wenqian Cao, Xiaotian Liu, Yunpeng Gai, Chenjia Shen, Huizhong Wang, Mingshuang Wang
Journal of Fungi.2024; 10(5): 360. CrossRef
Functional Plasticity, Redundancy, and Specificity of Lanosterol 14α-Demethylase in Regulating the Sensitivity to DMIs in Calonectria ilicicola
Lingling Wei, Haiping Shi, Bin Chen, Xiujuan Li, Wenchan Chen, Chengdong Wu, Yunpeng Gai, Changjun Chen
Journal of Agricultural and Food Chemistry.2024; 72(15): 8444. CrossRef
Molecular Mechanisms and Biological Characteristics of Botrytis cinerea Field Isolate Resistance to Pyrisoxazole in Liaoning Province
Le Chen, Bai-xin Sun, Yang Zhao, Ze-yan Miao
Plant Disease.2024; 108(4): 866. CrossRef
Overexpression of the CcCYP51A and CcCYP51B genes confer Corynespora cassiicola resistance to prochloraz
Yunyan Deng, Tao Wang, LuLu Zhang, Jiaxin Wang, Zhiqiu Qi, Mingshan Ji
Pesticide Biochemistry and Physiology.2024; 205: 106132. CrossRef
Field resistance to boscalid and molecular mechanisms of strawberry Botrytis cinerea in Chongqing, China
Pengfei Li, Xueru Yin, Yuheng Yang, Yang Yu, Anfei Fang, Binnian Tian, Chaowei Bi
Australasian Plant Pathology.2024; 53(1): 103. CrossRef
The 2023 update of target site mutations associated with resistance to fungicides and a web-tool to assist label designations
Richard Oliver, James Hane, Wesley Mair, Fran Lopez-Ruiz
Journal of Plant Diseases and Protection.2024; 131(4): 1265. CrossRef
Targeted deletion of three CYP51s in Fusarium fujikuroi and their different roles in determining sensitivity to 14α‐demethylase inhibitor fungicides
Cheng‐Xin Mao, Ju Luo, Yu Zhang, Chuan‐Qing Zhang
Pest Management Science.2023; 79(4): 1324. CrossRef
Detection and characterization of difenoconazole resistance in Stagonosporopsis citrulli from watermelon and muskmelon in Zhejiang Province of China
Zhangliang Kong, Yu Zhang, Chaojie Zhuang, Chenxin Mao, Chuanqing Zhang
Phytopathology Research.2023;[Epub] CrossRef
Actinomycins produced by endophyte Streptomyces sp. GLL‐9 from navel orange plant exhibit high antimicrobial effect against Xanthomonas citri susp. citri and Penicillium italicum
Liangliang Gao, Kaliaperumal Kumaravel, Qin Xiong, Yan Liang, Zhiran Ju, Yueming Jiang, Jun Zhang
Pest Management Science.2023; 79(11): 4679. CrossRef
Synergistic effect of amino acid substitutions in CYP51B for prochloraz resistance in Fusarium fujikuroi
FangJing Li, Akio Ebihara, Yuri Sakahara, Sarara Matsumoto, Reina Ueno, WanXue Bao, Makoto Kimura, Shin-ichi Fuji, Masafumi Shimizu, Koji Kageyama, Haruhisa Suga
Pesticide Biochemistry and Physiology.2023; 189: 105291. CrossRef
Characterization of Prochloraz Resistance in Fusarium fujikuroi from Heilongjiang Province in China
Qin Peng, Muhammad Waqas Younas, Jikun Yang, Hongwei Zhu, Jianqiang Miao, Biao Gu, Xili Liu
Plant Disease.2022; 106(2): 418. CrossRef
Risk and molecular mechanisms for boscalid resistance in Penicillium digitatum
Qianru Xu, Chaoxi Luo, Yanping Fu, Fuxing Zhu
Pesticide Biochemistry and Physiology.2022; 184: 105130. CrossRef
Bioactivity of mefentrifluconazole against different Fusarium spp.
Yahui Liu, Tianling Ma, Yi Dong, Chenxin Mao, Jianyan Wu, Chuanqing Zhang
Pesticide Biochemistry and Physiology.2022; 186: 105169. CrossRef
The Transcription Factor FgAtrR Regulates Asexual and Sexual Development, Virulence, and DON Production and Contributes to Intrinsic Resistance to Azole Fungicides in Fusarium graminearum
Yanxiang Zhao, Huilin Sun, Jingwen Li, Chao Ju, Jinguang Huang
Biology.2022; 11(2): 326. CrossRef
The antifungal activity of o-vanillin against Aspergillus flavus via disrupting ergosterol biosynthesis and promoting oxidative stress, and an RNA-seq analysis thereof
Qian Li, Xiaoman Zhu, Ying Zhao, Yanli Xie
LWT.2022; 164: 113635. CrossRef
The FgCYP51B Y123H Mutation Confers Reduced Sensitivity to Prochloraz and Is Important for Conidiation and Ascospore Development in Fusarium graminearum
Yanxiang Zhao, Mengyu Chi, Hunlin Sun, Hengwei Qian, Jun Yang, Jinguang Huang
Phytopathology®.2021; 111(8): 1420. CrossRef
Analysis of the prochloraz‐Mn resistance risk and its molecular basis in Mycogone rosea from Agaricus bisporus
Yixin Du, Niuniu Shi, Hongchun Ruan, Jianqiang Miao, He Yan, Chunxi Shi, Furu Chen, Xili Liu
Pest Management Science.2021; 77(10): 4680. CrossRef
Molecular Mechanisms Underlying Fungicide Resistance in Citrus Postharvest Green Mold
Paloma Sánchez-Torres
Journal of Fungi.2021; 7(9): 783. CrossRef
Baseline sensitivity and fungicidal action of propiconazole against Penicillium digitatum
Jun Zhang, Bao Zhang, Fuxing Zhu, Yanping Fu
Pesticide Biochemistry and Physiology.2021; 172: 104752. CrossRef
Aspergillus fumigatus Cross-Resistance between Clinical and Demethylase Inhibitor Azole Drugs
Rocio Garcia-Rubio, Irene Gonzalez-Jimenez, Jose Lucio, Emilia Mellado, Christopher A. Elkins
Applied and Environmental Microbiology.2021;[Epub] CrossRef
The completed genome sequence of the pathogenic ascomycete fungus Penicillium digitatum
Mingshuang Wang, Ruoxin Ruan, Hongye Li
Genomics.2021; 113(2): 439. CrossRef
Mutation in cyp51b and overexpression of cyp51a and cyp51b confer multiple resistant to DMIs fungicide prochloraz in Fusarium fujikuroi
Yu Zhang, Cheng‐Xin Mao, Xiao‐Yu Zhai, Pierce A Jamieson, Chuan‐Qing Zhang
Pest Management Science.2021; 77(2): 824. CrossRef
Fungicidal Actions and Resistance Mechanisms of Prochloraz to Penicillium digitatum
Yuchao Zhang, Bao Zhang, Chaoxi Luo, Yanping Fu, Fuxing Zhu
Plant Disease.2021; 105(2): 408. CrossRef
Reconstruction of a Context-Specific Model Based on Genome-Scale Metabolic Simulation for Identification of Prochloraz Resistance Mechanisms in Penicillium digitatum
Piao Zou, Yunze Zhang, Jean Bosco Nshimiyimana, Qianwen Cao, Yang Yang, Hui Geng, Li Xiong
Microbial Drug Resistance.2021; 27(6): 776. CrossRef
High Azole Resistance in Aspergillus fumigatus Isolates from Strawberry Fields, China, 2018
Yong Chen, Fengshou Dong, Jingya Zhao, Hong Fan, Chunping Qin, Runan Li, Paul E. Verweij, Yongquan Zheng, Li Han
Emerging Infectious Diseases.2020; 26(1): 81. CrossRef
Transcriptome analysis of fungicide-responsive gene expression profiles in two Penicillium italicum strains with different response to the sterol demethylation inhibitor (DMI) fungicide prochloraz
Tingfu Zhang, Qianwen Cao, Na Li, Deli Liu, Yongze Yuan
BMC Genomics.2020;[Epub] CrossRef
A Cyp51B Mutation Contributes to Azole Resistance in Aspergillus fumigatus
Irene Gonzalez-Jimenez, Jose Lucio, Jorge Amich, Isabel Cuesta, Rafael Sanchez Arroyo, Laura Alcazar-Fuoli, Emilia Mellado
Journal of Fungi.2020; 6(4): 315. CrossRef
Parallel evolution of multiple mechanisms for demethylase inhibitor fungicide resistance in the barley pathogen Pyrenophora teres f. sp. maculata
Wesley J. Mair, Geoffrey J. Thomas, Kejal Dodhia, Andrea L. Hills, Kithsiri W. Jayasena, Simon R. Ellwood, Richard P. Oliver, Francisco J. Lopez-Ruiz
Fungal Genetics and Biology.2020; 145: 103475. CrossRef
Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous Fusarium Isolates Obtained from Cereal Grains in Southern Poland
Teresa Krzyśko-Łupicka, Sławomir Sokół, Anna Piekarska-Stachowiak
Molecules.2020; 25(2): 292. CrossRef
Significance of 195 bp-enhancer of PdCYP51B in the acquisition of Penicillium digitatum DMI resistance and increase of fungal virulence
Marta de Ramón-Carbonell, Paloma Sánchez-Torres
Pesticide Biochemistry and Physiology.2020; 165: 104522. CrossRef
Global transcriptomic responses orchestrate difenoconazole resistance in Penicillium spp. causing blue mold of stored apple fruit
Franz J. Lichtner, Verneta L. Gaskins, Kerik D. Cox, Wayne M. Jurick
BMC Genomics.2020;[Epub] CrossRef
Blue-White Colony Selection of Virus-Infected Isogenic Recipients Based on a Chrysovirus Isolated from Penicillium italicum
Tingfu Zhang, Na Li, Yongze Yuan, Qianwen Cao, Yanfen Chen, Binglan Tan, Guoqi Li, Deli Liu
Virologica Sinica.2019; 34(6): 688. CrossRef
Cytosporone B as a Biological Preservative: Purification, Fungicidal Activity and Mechanism of Action against Geotrichum citri-aurantii
Chunxiao Yin, Hongxin Liu, Yang Shan, Vijai Kumar Gupta, Yueming Jiang, Weimin Zhang, Haibo Tan, Liang Gong
Biomolecules.2019; 9(4): 125. CrossRef
Elevated MIC Values of Imidazole Drugs against Aspergillus fumigatus Isolates with TR 34 /L98H/S297T/F495I Mutation
Yong Chen, Zongwei Li, Xuelin Han, Shuguang Tian, Jingya Zhao, Fangyan Chen, Xueting Su, Jingjun Zhao, Ziying Zou, Yanwen Gong, Fen Qu, Guangbin Qiu, Siyao Wang, Xiaodong Jia, Zhongyi Lu, Mandong Hu, Liuyu Huang, Paul E. Verweij, Li Han
Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef
Molecular and biological characteristics of laboratory metconazole-resistant mutants in Fusarium graminearum
Yabing Duan, Meixia Li, Huahua Zhao, Fei Lu, Jianxin Wang, Mingguo Zhou
Pesticide Biochemistry and Physiology.2018; 152: 55. CrossRef
Pharmacophore-Based Virtual Screening of Novel Inhibitors and Docking Analysis for CYP51A from Penicillium italicum
Yongze Yuan, Rui Han, Qianwen Cao, Jinhui Yu, Jiali Mao, Tingfu Zhang, Shengqiang Wang, Yuhui Niu, Deli Liu
Marine Drugs.2017; 15(4): 107. CrossRef
Classification of imazalil resistance in an international collection of Penicillium digitatum isolates
Mareli Kellerman, Adéle Mcleod, Ilze Beukes, Lindy J. Rose, Arno Erasmus, Paul H. Fourie
Canadian Journal of Plant Pathology.2017; 39(2): 133. CrossRef
Molecular bases of antifungal resistance in filamentous fungi
Cheshta Sharma, Anuradha Chowdhary
International Journal of Antimicrobial Agents.2017; 50(5): 607. CrossRef
A novel major facilitator superfamily transporter in Penicillium digitatum (PdMFS2) is required for prochloraz resistance, conidiation and full virulence
Zhi Wu, Shengqiang Wang, Yongze Yuan, Tingfu Zhang, Jing Liu, Deli Liu
Biotechnology Letters.2016; 38(8): 1349. CrossRef
Demethylase Inhibitor Fungicide Resistance in Pyrenophora teres f. sp. teres Associated with Target Site Modification and Inducible Overexpression of Cyp51
Wesley J. Mair, Weiwei Deng, Jonathan G. L. Mullins, Samuel West, Penghao Wang, Naghmeh Besharat, Simon R. Ellwood, Richard P. Oliver, Francisco J. Lopez-Ruiz
Frontiers in Microbiology.2016;[Epub] CrossRef
Transcriptional profiling analysis of Penicillium digitatum, the causal agent of citrus green mold, unravels an inhibited ergosterol biosynthesis pathway in response to citral
Qiuli OuYang, Nengguo Tao, Guoxing Jing
BMC Genomics.2016;[Epub] CrossRef
A Novel Sterol Regulatory Element-Binding Protein Gene (sreA) Identified in Penicillium digitatum Is Required for Prochloraz Resistance, Full Virulence and erg11 (cyp51) Regulation
Jing Liu, Yongze Yuan, Zhi Wu, Na Li, Yuanlei Chen, Tingting Qin, Hui Geng, Li Xiong, Deli Liu, Robert A. Cramer
PLOS ONE.2015; 10(2): e0117115. CrossRef
Azole fungicides - understanding resistance mechanisms in agricultural fungal pathogens
Claire L Price, Josie E Parker, Andrew GS Warrilow, Diane E Kelly, Steven L Kelly
Pest Management Science.2015; 71(8): 1054. CrossRef
Whole transcriptome analysis of Penicillium digitatum strains treatmented with prochloraz reveals their drug-resistant mechanisms
Jing Liu, Shengqiang Wang, Tingting Qin, Na Li, Yuhui Niu, Dandan Li, Yongze Yuan, Hui Geng, Li Xiong, Deli Liu
BMC Genomics.2015;[Epub] CrossRef

NOTE] Penicillium daejeonium sp. nov., a New Species Isolated from a Grape and Schisandra Fruit in Korea: Hyunkyu Sang , Tae-Jin An , Chang Sun Kim , Young Phil Choi , Jian-Xin Deng , Narayan Chandra Paul , Gi-Ho Sung , Seung Hun Yu; J. Microbiol. 2013;51(4):536-539. Published online August 30, 2013; DOI: https://doi.org/10.1007/s12275-013-3291-6

25 View
0 Download
11 Scopus

Abstract: Two isolates of monoverticillate Penicillium species were collected from a grape and schisandra fruit in Korea. Multigene phylogenetic analyses with the nuclear ribosomal internal transcribed spacer (ITS) region and genes encoding β-tubulin (benA) and calmodulin (cmd), as well as morphological analyses revealed that the two isolates are members of the P. sclerotiorum complex in Penicillium subgenus Aspergilloides, but different from species of the P. sclerotiorum complex. The isolates are closely related to P. cainii, P. jacksonii, and P. viticola in terms of their multigene phylogeny, but their colony and conidiophore morphologies differ from those of closely related species. The name P. daejeonium is proposed for this unclassified new species belonging to the P. sclerotiorum complex in subgenus Aspergilloides.

NOTE] The Microbial Population in the Air of Cultivation Facility of Oyster Mushrooms: Se Chul Chun , Yu Na Ahn , Sajid Mohamad Khan , Il Min Chung , Hyang Yoen Won , Chang Sung Jhune , Yool Jin Park; J. Microbiol. 2012;50(6):1053-1057. Published online December 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2195-1

25 View
0 Download
11 Scopus

Abstract: The microbial population in the air of mushroom cultivation facility was studied to understand the population structure and size depending on the cultivation methods and regions. The air contents of ten farmers’ oyster mushroom cultivation facilities in Kyunggi province were sampled. The results indicated that there was no difference in population size depending on the regions of mushroom cultivation. In addition, the population size of bacteria in the growth room was bigger than that of the cooling room and outside of the mushroom house, but the fungal population was similar in size between cultivation stages. With regard to population structure, Pseudomonas and Penicillium species were most frequently isolated from the air of oyster mushroom cultivation facility.

Characterization of Cellulases of Fungal Endophytes Isolated from Espeletia spp.: Luisa Cabezas , Carolina Calderon , Luis Miguel Medina , Isabela Bahamon , Martha Cardenas , Adriana Jimena Bernal , Andrés Gonzalez , Silvia Restrepo; J. Microbiol. 2012;50(6):1009-1013. Published online December 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2130-5

27 View
0 Download
20 Scopus

Abstract: Endophytes are microorganisms that asymptomatically invade plant tissues. They can stimulate plant growth and/or provide defense against pathogen attacks through the production of secondary metabolites. Most endophyte species are still unknown, and because they may have several applications, the study of their metabolic capabilities is essential. We characterized 100 endophytes isolated from Espeletia spp., a genus unique to the paramo ecosystem, an extreme environment in the Andean mountain range. We evaluated the cellulolytic potential of these endophytes on the saccharification of the oil palm empty fruit bunch (OPEFB). The total cellulolytic activity was measured for each endophyte on filter paper (FPA). In addition, the specific carboxymethyl cellulase (CMCase), exoglucanase, and β-glucosidase activities were determined. We found four fungi positive for cellulases. Of these fungi, Penicillium glabrum had the highest cellulolytic activity after partial purification, with maximal CMCase, exoglucanase and β-glucosidase enzyme activities of 44.5, 48.3, and 0.45 U/ml, respectively. Our data showed that the bioprospection of fungi and the characterization of their enzymes may facilitate the process of biofuel production.

Journal Article

Organic Acids Associated with Saccharification of Cellulosic Wastes During Solid-State Fermentation: Noura El-Ahmady El-Naggar , Mohammed Saad El-Hersh; J. Microbiol. 2011;49(1):58-65. Published online March 3, 2011; DOI: https://doi.org/10.1007/s12275-011-0288-x

40 View
0 Download
13 Scopus

Abstract: Saccharification of five cellulosic wastes, i.e. rice husks, wheat bran, corn cobs, wheat straw and rice straw by three cellulytic fungi, i.e. Aspergillus glaucus MN1, Aspergillus oryzae MN2 and Penicillium purpurogenum MN3, during solid-state fermentation (SSF) was laboratory studied. Rice husks, wheat bran, and corn cobs were selected as inducers of glucose production in the tested fungi. An incubation interval of 10 days was optimal for glucose production. Maximal activities of the cellulases FP-ase, CMC-ase, and β-glucosidase were detected during SSF of rice husks by P. purpurogenum; however, α-amylase activity (7.2 U/g) was comparatively reduced. Meanwhile, the productivities of FP-ase, CMC-ase, and β-glucosidase were high during SSF of rice husks by A. glaucus; however, they decreased during SSF of corn cobs by P. purpurogenum. Addition of rock phosphate (RP) (75 mg P2O5) decreased the pH of SSF media. (NH4)2SO4 was found to be less inducer of cellulytic enzymes, during SSF of rice husks by A. glaucus or A. oryzae; it also induced phytase production and solubilization of RP. The organic acids associated with saccharification of the wastes studied have also been investigated. The highest concentration of levulinic acid was detected (46.15 mg/g) during SSF of corn cobs by P. purpurogenum. Likewise, oxalic acid concentration was 43.20 mg/g during SSF of rice husks by P. purpurogenum.

Research Support, Non-U.S. Gov't

Functional Characterization of the Copper-Transporting P-Type ATPase Gene of Penicillium janthinellum Strain GXCR: Hongmin Lai , Changbin Sun , Huaying Tang , Xianwei Fan , Yili Ma; J. Microbiol. 2009;47(6):736-745. Published online February 4, 2010; DOI: https://doi.org/10.1007/s12275-009-0074-1

42 View
0 Download
3 Scopus

Abstract: Copper (Cu)-transporting P-type ATPase (CTPA) genes have been documented to play an important role in resistance to heavy metals. However, our understanding of roles of CTPA genes of the filamentous fungi was based only on sequence similarity prediction before. In a previous study, we isolated a Penicillum janthinellum strain GXCR of higher tolerance to Cu (200 mM). In this study, we cloned the partial cDNA of CTPA gene, named PcpA, from the strain GXCR. Sequence alignment indicated that the cloned cDNA sequence has the highest identity (94.4%) with a predictive CTPA gene of Aspergillus clavatus. The PcpA- encoded protein, termed PcpA, has classical functional domains of CTPAs, and shows differences from reported CTPAs in some specific sequence motifs and transmembrane regions. Expression of the PcpA was induced by extracellular Cu, cadmium (Cd), and silver (Ag). PcpA RNA interference (RNAi) mutants with a reduced level of PcpA mRNA were more sensitive to Cu, iron, Cd, and Ag than the wild-type (WT) strain GXCR. When grown in the presence of Cu, iron, and Cd, intracellular Cu and iron contents in the PcpA RNAi mutant were significantly (P<0.05) lower than those in the WT; However, intracellular Cd content in the mutant was significantly (P<0.05) higher than that in the WT. Taken together, it can be concluded that the PcpA functions in Cu uptake and homeostasis, iron uptake, and Cd export from the cytosol to the extracytosol.

Genetic Relationships among Penicillium Species by Characterizing RAPD Markers: Yoon, Cheol Sik , Bae, Kyung Sook; J. Microbiol. 1995;33(3):171-177.

42 View
0 Download

Abstract: Random amplified polymorphic DAN markers were characterized for three taxonomically problematic Penicillium species : P. aurantiogriseum var. Aurantiogriseum, P. verrucosum and P. puberulum, as well as for 25 species of mono, bi-, and terverticillate Penicillia. The relationships among mono, bi-, and terverticillate Penicillium species were determined from these RAPD markers. Eight species from mono-, eight from bi-, and nine from terverticilate Penicillia were examined. With 14 randomly chosen 10-mer primes, a 310 character by 25 species matrix was generated. Phenetic analysis separated the 25 species into three genetically distinct groups that correspond to the different arrangements of penicilli (mono-, bi-, and terverticillate). The results of this study suggest that P. aurantiogriseum var. aurantiogriseum, P. VERRUCOSUM, AND P. puberulum represent genetically distinct species, and that P. vulpinum should be included in terverticilate Penicillia. Phenogram branching patterns indicated that biverticillate species are genetically more similar to monoverticilate species than they are to terverticillate species.

Purification of carbosymethyl cellulase from hybrid between aspergillus niger and penicillium verruculosum: Yang, Young Ki , Lee, Jung Sup , Park, Hyung Nam , Moon, Myung Nim , Kim, Hong Sub , Kim, Jong Se , Lim, Chae Young , Rhee, Young Ha; J. Microbiol. 1996;34(1):90-94.

36 View
0 Download

Abstract: The carboxymethyl cellulase (CMCase) was purified from the induced culture filtrate of hybrid TAPW15703 between Aspergillus niger and penicillium verruculosum made by nuclear transfer. The enzyme was purified 80 fold with an overall yield 17% from the culture medium by ammonium sulfate fractionation, Sephadex G-75 gel permeation chromatography, and DEAE-ion exchange column chromatography. The molecular weight of the CMCase has estimated to be 32,000 daltons on SDS-polyacrylamide gel electrophoresis and Sephadex G-150 gel permeation chromatography. The purified enzyme functions optimally at pH 4.0 and 40℃. The Km value for carbosymethyl cellulose was 68 mM. The enzyme activity was increased by the presence of Mg^2+ and Mn^2+.

Phylogenetic study of penicillium chrysogenum based on the amino acid sequence analysis of chitin synthase: Park, Bum Chan , Lee, Dong Hun , Bae, Kyung Sook , Park, Hee Moon; J. Microbiol. 1997;35(3):159-164.

37 View
0 Download

Abstract: The phylogenetic study of Penicilium chrysogenum was performed based on amino acid sequence comparison of chitin synthase. Phylogenetic trees were constructed with the deduced amino acid sequences of the highly conserved region of chitin synthase gene fragments amplified by PCR. The BlasP similarity search and the bootstrap analysis of the deduced amino acid sequences of chitin synthase from P. chrysogenum with those form other fungi showed a close evolutionary relationship of Penicillium to ascomycetous fungi, especially to genus Aspergilus. The result from bootstrap analysis of the deduced amino acid sequences of the Class II chitin synthase from ascomyceteous fungi supported the usefulness of the Class II chitin synthease for phylogenetic study of filamentous fungi.

Purification of an Immunosuppressor Produced by Penicillium urticae: Lee, Jae Jung , Yoo, Seoung Ku , Kim, Eui Joong , Yu, Ju Hyun , Bai, Dong Hoon , Yoon, Sung Sik , Kim, Wook Sung; J. Microbiol. 1998;36(4):280-282.

31 View
0 Download

Abstract: An immunosuppressive chemical produced by Penicilium urticae (KFCC 11611) was purified from the supernatant of a culture broth by ethyl acetate extraction, silica gel column chromatography, and precipitation by adding cold n-hexane followed by crystalization. The chemical effectively inhibited the mouse mixed lymphocyte reaction (MLR) at a concentration of 1.25ⅹ10^3 ㎍/ml, which was lower than the effective concentration of cyclosporin A. The chemical was identified as the antibiotic patulin (MW 154.1 Da) by NMR and mass spectroscopy.

First
Prev
Page of 2
Next
Last

Search