Journal of Microbiology

Research Support, Non-U.S. Gov'ts

NOTE] Regulatory Characteristics of the Vibrio vulnificus rtxHCA Operon Encoding a MARTX Toxin: Jinseo Park , Seung Min Kim , Hee Gon Jeong , Sang Ho Choi; J. Microbiol. 2012;50(5):878-881. Published online September 21, 2012; DOI: https://doi.org/10.1007/s12275-012-2479-5

Abstract: Vibrio vulnificus MARTX encoded by rtxA, an open reading frame of the rtxHCA operon, is essential for virulence in vitro and in mice. In this study, a primer extension analysis revealed that transcription of the rtxHCA operon begins at a single site, and is under the direction of a single promoter, PrtxHCA. PrtxHCA activity appeared at the beginning of growth and reached a maximum in mid-exponential phase. PrtxHCA activity was induced by exposure to INT-407 cells, and the membrane fraction of INT-407 cells was the most effective for the induction.

Identification and Characterization of the Vibrio vulnificus rtxA Essential for Cytotoxicity in vitro and Virulence in Mice: Jeong Hyun Lee , Myung Won Kim , Byoung Sik Kim , Seung Min Kim , Byung Cheol Lee , Tae Sung Kim , Sang Ho Choi; J. Microbiol. 2007;45(2):146-152.; DOI: https://doi.org/2520 [pii]

Abstract: A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.

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