Journal of Microbiology

Characterization of a Streptomycete Isolate Producing the Potent Cytotoxic Substance, Nonadecanoic Acid: Jin-cheol Yoo , Ji-Man Han , Seung-Kwan Nam , Keun-shik Baik , Jung-sun Jo , Chi-nam Seong; J. Microbiol. 2002;40(2):178-181.

Abstract: Streptomycete isolate, strain M0137 showed cytotoxic effect on THP-1 cells. One of the purified substances produced from the strain was identified as nonadecanoic acid. Morphological and physiological properties, phylogenetic analysis, and genomic fingerprinting of strain M0137 were determined. Strain M0137 showed a high similarity with Streptomyces scabiei, phenotypically and phylogenetically. In contrast, genomic fingerprinting and G+C content analysis revealed that strain M0137 could be distinguished from S. scabiei ATCC49173 T . We propose to name strain M0137 as Streptomyces scabiei subsp. chosunensis.

Characterization and Cytotoxic Activities of Nonadecanoic Acid Produced by Streptomyces scabiei subsp. chosunensis M0137 (KCTC 9927): Jin-Cheol Yoo , Ji-Man Han , Seung-Kwan Nam , Ok-Hyun Ko , Cheol-Hee Choi , Keun-Hong Kee , Jae-Kyung Sohng , Jung-Sun Jo , Chi-Nam Seong; J. Microbiol. 2002;40(4):331-334.

Abstract: The substance 0116p, which exhibits cytotoxicity against human macrophage cell line THP-1, was isolated from a mycelial extract of Streptomyces scabiei subsp. chosunensis M0137. The cytotoxic substance was purified by Diaion-HP20 adsorption, solvent extraction, Sephadex LH-20 column chromatography, and silica-gel column chromatography. The molecular formula is C_19 H_38 O_2 (MW 301.10) based on elemental and spectrometric analysis. It was identified as nonadecanoic acid by NMR spectral data. It exhibits cytotoxic activities in various human cancer cell lines, including A549, SK-OV-3, SK-MEL-2 and HCT-15. In addition, 0116p also inhibits IL-12 production in lipopolysaccharide-activated macrophages.

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