Journal of Microbiology

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Mannose phosphotransferase system subunit IID of Streptococcus mutans elicits maturation and activation of dendritic cells: Sungho Jeong, Chaeyeon Park, Dongwook Lee, Hyun Jung Ji, Ho Seong Seo, Cheol-Heui Yun, Jintaek Im, Seung Hyun Han; J. Microbiol. 2025;63(10):e2505014. Published online October 31, 2025; DOI: https://doi.org/10.71150/jm.2505014

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Abstract PDF: Streptococcus mutans is a Gram-positive pathogen that causes dental caries and subsequent pulpal infection leading to pulpitis. Although dendritic cells (DCs) are known to be involved in disease progression and immune responses during S. mutans infection, little is known about which component of S. mutans is responsible for the DC responses. Although the mannose phosphotransferase system (Man-PTS) is the primary sugar transporter of S. mutans, it is also a potential virulence factor. Since Man-PTS subunit IID (ManIID) embedded on the bacterial membrane is indispensable for Man-PTS function, we investigated its role in the maturation and activation of DCs stimulated with a ManIID-deficient strain (Δpts) of S. mutans and recombinant ManIID (rManIID) protein. When mouse bone marrow-derived DCs were treated with heat-killed S. mutans wild-type (WT) or Δpts, bacterial adherence and internalization of Δpts were lower than those of WT. Moreover, the heat-killed S. mutans Δpts strain was inferior to the wild-type in inducing expression of phenotypic maturation markers, such as CD80, CD86, MHC-I, and MHC-II, and proinflammatory cytokine, IL-6. In line with the trends in marker expression, the endocytic capacity of DCs treated with the Δpts strain was comparable to that of untreated DCs whereas DCs treated with the WT strain dose-dependently lost their endocytic capacity. Furthermore, rManIID dose-dependently promoted both phenotypic maturation marker expression and IL-6 production by DCs. Collectively, these results demonstrate that ManIID plays a crucial role in the adhesion and internalization of S. mutans into DCs and is one of the major immune-stimulating agents responsible for maturation and activation of DCs during S. mutans infection.

Journal Article

Lactobacillus curvatus WiKim38 isolated from kimchi induces IL-10 production in dendritic cells and alleviates DSS-induced colitis in mice: Sung-Gang Jo , Eui-Jeong Noh , Jun-Young Lee , Green Kim , Joo-Hee Choi , Mo-Eun Lee , Jung-Hee Song , Ji-Yoon Chang , Jong-Hwan Park; J. Microbiol. 2016;54(7):503-509. Published online June 28, 2016; DOI: https://doi.org/10.1007/s12275-016-6160-2

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Probiotics such as lactobacilli and bifidobacteria have healthpromoting effects by immune modulation. In the present study, we examined the immunomodulatory properties of Lactobacillus curvatus WiKim38, which was newly isolated from baechu (Chinese cabbage) kimchi. The ability of L. curvatus WiKim38 to induce cytokine production in bone marrow-derived dendritic cells (BMDCs) was determined by enzyme-linked immunosorbent assay. To evaluate the molecular mechanisms underlying L. curvatus Wikim38-mediated IL-10 production, Western blot analyses and inhibitor assays were performed. Moreover, the in vivo anti-inflammatory effects of L. curvatus WiKim38 were examined in a dextran sodium sulfate (DSS)-induced colitis mouse model. L. curvatus WiKim38 induced significantly higher levels of IL- 10 in BMDCs compared with that induced by LPS. NF-κB and ERK were activated by L. curvatus WiKim38, and an inhibitor assay revealed that these pathways were required for L. curvatus WiKim38-induced production of IL-10 in BMDCs. An in vivo experiment showed that oral administration of L. curvatus WiKim38 increased the survival rate of mice with DSS-induced colitis and improved clinical signs and histopathological severity in colon tissues. Taken together, these results indicate that L. curvatus Wikim38 may have health-promoting effects via immune modulation, and may thus be applicable for therapy of various inflammatory diseases.

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Immunostimulatory Activity of Dendritic Cells Pulsed with Carbonic Anhydrase IX and Acinetobacter baumannii Outer Membrane Protein A for Renal Cell Carcinoma: Bo Ra Kim , Eun Kyoung Yang , Sun Hee Kim , Dong Chan Moon , Hwa Jung Kim , Je Chul Lee , Duk Yoon Kim; J. Microbiol. 2011;49(1):115-120. Published online March 3, 2011; DOI: https://doi.org/10.1007/s12275-011-1037-x

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Abstract PDF

Dendritic cell (DC)-based immunotherapy is a potent therapeutic modality for treating renal cell carcinoma (RCC), but development of antigens specific for tumor-targeting and anti-tumor immunity is of great interest for clinical trials. The present study investigated the ability of DCs pulsed with a combination of carbonic anhydrase IX (CA9) as an RCC-specific biomarker and Acinetobacter baumannii outer membrane protein A (AbOmpA) as an immunoadjuvant to induce anti-tumor immunity against murine renal cell carcinoma (RENCA) in a murine model. Murine bone-marrow-derived DCs pulsed with a combination of RENCA lysates and AbOmpA were tested for their capacity to induce DC maturation and T cell responses in vitro. A combination of RENCA lysates and AbOmpA up-regulated the surface expression of co-stimulatory molecules, CD80 and CD86, and the antigen presenting molecules, major histocompatibility (MHC) class I and class II, in DCs. A combination of RENCA lysates and AbOmpA also induced interleukin-12 (IL-12) production in DCs. Next, the immunostimulatory activity of DCs pulsed with a combination of CA9 and AbOmpA was determined. A combination of CA9 and AbOmpA up-regulated the surface expression of co-stimulatory molecules and antigen presenting molecules in DCs. DCs pulsed with a combination of CA9 and AbOmpA effectively secreted IL-12 but not IL-10. These cells interacted with T cells and formed clusters. DCs pulsed with CA9 and AbOmpA elicited the secretion of interferon-γ and IL-2 in T cells. In conclusion, a combination of CA9 and AbOmpA enhanced the immunostimulatory activity of DCs, which may effectively induce anti-tumor immunity against human RCC.

Citations

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The Outer Membrane Proteins OmpA, CarO, and OprD of Acinetobacter baumannii Confer a Two-Pronged Defense in Facilitating Its Success as a Potent Human Pathogen
Siva R. Uppalapati, Abhiroop Sett, Ranjana Pathania
Frontiers in Microbiology.2020;[Epub] CrossRef
Host Innate Immune Responses to Acinetobacter baumannii Infection
Wangxue Chen
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef
Generation of anti-tumour immune response using dendritic cells pulsed with carbonic anhydrase IX-Acinetobacter baumanniiouter membrane protein A fusion proteins against renal cell carcinoma
B-R Kim, E-K Yang, D-Y Kim, S-H Kim, D-C Moon, J-H Lee, H-J Kim, J-C Lee
Clinical and Experimental Immunology.2011; 167(1): 73. CrossRef

Anti-Tumor Activity of Acinetobacter baumannii Outer Membrane Protein A on Dendritic Cell-Based Immunotherapy against Murine Melanoma: Jun Sik Lee , Jung Wook Kim , Chul Hee Choi , Won Kee Lee , Hae Young Chung , Je Chul Lee; J. Microbiol. 2008;46(2):221-227. Published online June 11, 2008; DOI: https://doi.org/10.1007/s12275-008-0052-z

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Abstract PDF: Acinetobacter baumannii outer membrane protein A (AbOmpA) is a major surface protein that is an important pathogen-associated molecular pattern. Based on our previous findings that AbOmpA induced the phenotypic maturation of dendritic cells (DCs) and drove the Th1 immune response in vitro, we investigated the therapeutic efficacy of AbOmpA-pulsed DC vaccines in a murine melanoma model. The surface expression of co-stimulatory molecules (CD80 and CD86) and major histocompatibility complex class I and II molecules was higher in DCs pulsed with AbOmpA alone or with a combination of B16F10 cell lysates than that of DCs pulsed with B16F10 cell lysates. AbOmpA stimulated the maturation of murine splenic DCs in vivo. In a therapeutic model of murine melanoma, AbOmpA-pulsed DCs significantly retarded tumor growth and improved the survival of tumor-bearing mice. AbOmpA-pulsed DCs significantly enhanced CD8+, interleukin-2+ T cells and CD4+, interferon-γ+ T cells in tumor-bearing mice. These results provide evidence that AbOmpA may be therapeutically useful in adjuvant DC immunotherapy against poorly immunogenic melanoma without tumor-specific antigens.

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