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MINIREVIEW] Rapid and robust MALDI-TOF MS techniques for microbial identification: a brief overview of their diverse applications
Kyoung-Soon Jang , Young Hwan Kim
J. Microbiol. 2018;56(4):209-216.   Published online February 28, 2018
DOI: https://doi.org/10.1007/s12275-018-7457-0
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AbstractAbstract
Advances in mass spectrometry have enabled the investigation of various biological systems by directly analyzing diverse sets of biomolecules (i.e., proteins, lipids, and carbohydrates), thus making a significant impact on the life sciences field. Over the past decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been widely utilized as a rapid and reliable method for the identification of microorganisms. MALDI-TOF MS has come into widespread use despite its relatively low resolving power (full width at half maximum, FWHM: < 5,000) and its incompatibility with tandem MS analysis, features with which other high-resolution mass spectrometers are equipped. Microbial identification is achieved by searching databases containing mass spectra of peptides and proteins extracted from microorganisms of interest, using scoring algorithms to match analyzed spectra with reference spectra. In this paper, we give a brief overview of the diverse applications of rapid and robust MALDI-TOF MS-based techniques for microbial identification in a variety of fields, such as clinical diagnosis and environmental and food monitoring. We also describe the fundamental principles of MALDI-TOF MS. The general specifications of the two major MS-based microbial identification systems available in the global market (BioTyper® and VITEK® MS Plus) and the distribution of these instruments in Republic of Korea are also discussed. The current review provides an understanding of this emerging microbial identification and classification technology and will help bacteriologists and cell biologists take advantage of this powerful technique.

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    Microbiology Spectrum.2024;[Epub]     CrossRef
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    Archives of Microbiology.2023;[Epub]     CrossRef
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    Abdessalam Cherkaoui, Arnaud Riat, Gesuele Renzi, Adrien Fischer, Jacques Schrenzel
    European Journal of Clinical Microbiology & Infectious Diseases.2023; 42(2): 153.     CrossRef
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    Natalija Topić Popović, Snježana P. Kazazić, Krunoslav Bojanić, Ivančica Strunjak‐Perović, Rozelindra Čož‐Rakovac
    Mass Spectrometry Reviews.2023; 42(5): 1589.     CrossRef
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    LWT.2023; 176: 114508.     CrossRef
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    Veterinary Research Communications.2023; 47(1): 73.     CrossRef
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    Journal of Clinical Microbiology.2020;[Epub]     CrossRef
  • Efficacy of low energy electron beam on microbial decontamination of spices
    Urszula Gryczka, Hiromi Kameya, Keitarou Kimura, Setsuko Todoriki, Wojciech Migdał, Sylwester Bułka
    Radiation Physics and Chemistry.2020; 170: 108662.     CrossRef
  • Identification of hyperglycemia-associated microbiota alterations in saliva and gingival sulcus
    Yu-Shan Wei, Ya-Chun Hsiao, Guan-Wei Su, Yi-Ru Chang, Hsiu-Pin Lin, Yi-Shiuan Wang, Yi-Ting Tsai, En-Chi Liao, Hsin-Yi Chen, Hsiu-Chuan Chou, Mei-Lan Ko, Wen-Hung Kuo, Shing-Jyh Chang, Wen-Chi Cheng, Hong-Lin Chan
    Archives of Biochemistry and Biophysics.2020; 682: 108278.     CrossRef
  • Recent update in diagnosis and treatment of human pythiosis
    Maria Nina Chitasombat, Passara Jongkhajornpong, Kaevalin Lekhanont, Theerapong Krajaejun
    PeerJ.2020; 8: e8555.     CrossRef
  • Application of MALDI-TOF MS for Rapid Identification of Microorganisms in Food Microbiology
    Hiroko Kawasaki
    Japanese Journal of Food Microbiology.2020; 37(4): 165.     CrossRef
  • MALDI-TOF mass spectrometry typing for predominant serovars of non-typhoidal Salmonella in a Thai broiler industry
    Suthee Mangmee, Onrapak Reamtong, Thareerat Kalambaheti, Sittiruk Roytrakul, Piengchan Sonthayanon
    Food Control.2020; 113: 107188.     CrossRef
  • Spontaneous bacterial peritonitis caused by Campylobacter Coli in cirrhotic patient
    Cheng-Hui Wang, Ting-Han Tai, Shih-Yen Weng, Shin-Wen Yeh, Sheng-Jie Shiue, Ganbolor Jargalsaikhan, Ming-Shun Wu
    Medicine.2020; 99(21): e19887.     CrossRef
  • Rapid screening of marine bacterial symbionts using MALDI-TOF MS
    Livia M. R. Vidal, Tainá M. Venas, Aline R. P. Gonçalves, Hannah K. Mattsson, Raphael V. P. Silva, Maria S. Nóbrega, Gustavo P. R. Azevedo, Gizele D. Garcia, Diogo A. Tschoeke, Verônica V. Vieira, Fabiano L. Thompson, Cristiane C. Thompson
    Archives of Microbiology.2020; 202(8): 2329.     CrossRef
  • Identification of pathogens from native urine samples by MALDI-TOF/TOF tandem mass spectrometry
    Damir Oros, Marina Ceprnja, Jurica Zucko, Mario Cindric, Amela Hozic, Jasenka Skrlin, Karmela Barisic, Ena Melvan, Ksenija Uroic, Blazenka Kos, Antonio Starcevic
    Clinical Proteomics.2020;[Epub]     CrossRef
  • Sensitive detection of quinoline-derivatized sitagliptin in small volumes of human plasma by MALDI-TOF mass spectrometry
    Yi-Shan Li, Wei-Lung Tseng, Chi-Yu Lu
    Talanta.2020; 218: 121143.     CrossRef
  • The management of anti-infective agents in intensive care units: the potential role of a ‘fast’ pharmacology
    Dario Cattaneo, Alberto Corona, Francesco Giuseppe De Rosa, Cristina Gervasoni, Danijela Kocic, Deborah Je Marriott
    Expert Review of Clinical Pharmacology.2020; 13(4): 355.     CrossRef
  • Identification of bacteria in juice/lettuce using magnetic nanoparticles and selected reaction monitoring mass spectrometry
    Cheng-Tung Chen, Je-Wei Yu, Yen-Peng Ho
    Journal of Food and Drug Analysis.2019; 27(2): 575.     CrossRef
  • Application of the Whole Genome-Based Bacterial Identification System, TrueBac ID, Using Clinical Isolates That Were Not Identified With Three Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems
    Sung-Min Ha, Chang Ki Kim, Juhye Roh, Jung-Hyun Byun, Seung-Jo Yang, Seon-Bin Choi, Jongsik Chun, Dongeun Yong
    Annals of Laboratory Medicine.2019; 39(6): 530.     CrossRef
  • Antimicrobial random peptide cocktails: a new approach to fight pathogenic bacteria
    Zaid Amso, Zvi Hayouka
    Chemical Communications.2019; 55(14): 2007.     CrossRef
  • Application of MALDI Biotyper System for Rapid Identification of Bacteria Isolated from a Fresh Produce Market
    Israa Mohamad El-Nemr, Mohanad Mushtaha, Sathyavathi Sundararaju, Charmaine Fontejon, Mohammed Suleiman, Patrick Tang, Ipek Goktepe, Mohammad Rubayet Hasan
    Current Microbiology.2019; 76(3): 290.     CrossRef
  • Identification of dermatophytes by MALDI-TOF MS technology in the clinical laboratory
    Maya Azrad, Victoria Freidus, Riad Kassem, Avi Peretz
    International Journal of Mass Spectrometry.2019; 440: 32.     CrossRef
  • Differences between Staphylococcus aureus lineages isolated from ovine and caprine mastitis but not between isolates from clinical or subclinical mastitis
    J. Hoekstra, V.P.M.G. Rutten, M. van den Hout, M.P. Spaninks, L. Benedictus, G. Koop
    Journal of Dairy Science.2019; 102(6): 5430.     CrossRef
  • MALDI–MS Profiling to Address Honey Bee Health Status under Bacterial Challenge through Computational Modeling
    Karim Arafah, Sébastien Nicolas Voisin, Victor Masson, Cédric Alaux, Yves Le Conte, Michel Bocquet, Philippe Bulet
    PROTEOMICS.2019;[Epub]     CrossRef
  • Rapid Antimicrobial Susceptibility Testing of Patient Urine Samples Using Large Volume Free-Solution Light Scattering Microscopy
    Manni Mo, Yunze Yang, Fenni Zhang, Wenwen Jing, Rafael Iriya, John Popovich, Shaopeng Wang, Thomas Grys, Shelley E. Haydel, Nongjian Tao
    Analytical Chemistry.2019; 91(15): 10164.     CrossRef
  • Advances in Chemical and Biological Methods to Identify Microorganisms—From Past to Present
    Ricardo Franco-Duarte, Lucia Černáková, Snehal Kadam, Karishma S. Kaushik, Bahare Salehi, Antonio Bevilacqua, Maria Rosaria Corbo, Hubert Antolak, Katarzyna Dybka-Stępień, Martyna Leszczewicz, Saulo Relison Tintino, Veruska Cintia Alexandrino de Souza, Ja
    Microorganisms.2019; 7(5): 130.     CrossRef
  • Rapid MALDI-TOF MS identification of commercial truffles
    Khalid El Karkouri, Carine Couderc, Philippe Decloquement, Annick Abeille, Didier Raoult
    Scientific Reports.2019;[Epub]     CrossRef
  • Association between a Genetic Risk Score Based on Single Nucleotide Polymorphisms of Coronary Artery Disease-Related Genes and Left Main Coronary Artery Disease
    Zhengxi Xu, Hanning Liu, Cheng Sun, Ke Si, Yan Zhao, Zhe Zheng
    BioMed Research International.2018; 2018: 1.     CrossRef
Journal Article
Identification of cyst wall proteins of the hypotrich ciliate Euplotes encysticus using a proteomics approach
Bangzheng Wang , Tao Niu , Muhammad Zeeshan Bhatti , Fenfen Chen , Lin Wu , Jiwu Chen
J. Microbiol. 2017;55(7):545-553.   Published online June 30, 2017
DOI: https://doi.org/10.1007/s12275-017-6422-7
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AbstractAbstract
Euplotes encysticus is a species of Hypotrich ciliates, which form cyst wall by secreting the special substances on encounter of adverse environment. It has critical significance to study the component and mechanism underlying resting cyst, during resisting unfavorable conditions in dormancy induction. The present study was aimed to investigate the effects of cyst wall proteins of Euplotes encysticus by using biochemical
methods
. Therefore, protein extracts were separated by SDSPAGE, identified and analyzed by MALDI-TOF MS and Bioinformatics tools. We detected 42 cyst wall proteins, 26 were functional proteins and 16 proteins consist of unknown function; which is consistent with cyst wall specificity. These results partially revealed the components of resting cyst wall formed after the cells differentiation of Euplotes encysticus. In addition, our data suggested that the function of cyst wall proteins are more likely involved in the mechanical protection, signal transduction, material transport, protein degradation and energy metabolism to survival, with potentially importance implications in the molecular mechanism of eukaryocyte dormancy under stress condition.

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Citations to this article as recorded by  
  • Decryption of the survival “black box”: gene family expansion promotes the encystment in ciliated protists
    Didi Jin, Chao Li, Xiao Chen, Yurui Wang, Khaled A. S. Al-Rasheid, Naomi A. Stover, Chen Shao, Tengteng Zhang
    BMC Genomics.2024;[Epub]     CrossRef
  • Role of chitin synthases CHS1 and CHS2 in biosynthesis of the cyst wall of Cryptocaryon irritans
    Huicheng Wu, Yihao Cen, Yipei Lu, Pengbo Dan, Yanwei Li, Xueming Dan, Zequan Mo
    International Journal of Biological Macromolecules.2024; 280: 136143.     CrossRef
  • Water temperature affects Cryptocaryon irritans development, cryptocaryoniasis occurrence and an auxiliary treatment decision-making webpage
    Fei Yin, Jing Yin, Xiao Xie, Linhua Jiang
    Aquaculture.2023; 574: 739694.     CrossRef
  • A light and electron microscopical study on the resting cyst of the tintinnid Schmidingerella (Alveolata, Ciliophora) including a phylogeny-aware comparison
    Maximilian H. Ganser, Heidi Bartel, Birgit Weißenbacher, Ancuela Andosch, Ursula Lütz-Meindl, Peter Radacher, Sabine Agatha
    European Journal of Protistology.2022; 86: 125922.     CrossRef
  • How Ciliated Protists Survive by Cysts: Some Key Points During Encystment and Excystment
    Yuqing Li, Yurui Wang, Shijing Zhang, Xyrus X. Maurer-Alcalá, Ying Yan
    Frontiers in Microbiology.2022;[Epub]     CrossRef
  • Early signaling pathways mediating dormant cyst formation in terrestrial unicellular eukaryoteColpoda
    Tatsuomi Matsuoka
    FEMS Microbiology Letters.2021;[Epub]     CrossRef
  • Novel insights into molecular mechanisms of Pseudourostyla cristata encystment using comparative transcriptomics
    Nan Pan, Tao Niu, Muhammad Zeeshan Bhatti, Haiyang Zhang, Xinpeng Fan, Bing Ni, Jiwu Chen
    Scientific Reports.2019;[Epub]     CrossRef
  • New contribution to the morphology and molecular mechanism of Euplotes encysticus encystment
    Fenfen Chen, Yanyan Xue, Nan Pan, Muhammad Zeeshan Bhatti, Tao Niu, Jiwu Chen
    Scientific Reports.2018;[Epub]     CrossRef
  • Morphological and Molecular Redefinition of Euplotes platystoma Dragesco & Dragesco‐Kernéis, 1986 and Aspidisca lynceus (Müller,1773) Ehrenberg, 1859, with Reconsideration of a “Well‐known” Euplotes Ciliate, Euplotes harpa Stein, 1859 (Ciliophora, Euploti
    Chunyu Lian, Xiaotian Luo, Xinpeng Fan, Jie Huang, Yuhe Yu, William Bourland, Weibo Song
    Journal of Eukaryotic Microbiology.2018; 65(4): 531.     CrossRef
  • The affiliation of Hexasterias problematica and Halodinium verrucatum sp. nov. to ciliate cysts based on molecular phylogeny and cyst wall composition
    Pieter R. Gurdebeke, Kenneth Neil Mertens, Yoshihito Takano, Aika Yamaguchi, Kara Bogus, Micah Dunthorn, Kazumi Matsuoka, Henk Vrielinck, Stephen Louwye
    European Journal of Protistology.2018; 66: 115.     CrossRef
  • Ultrastructure of vegetative cells and resting cysts, and live observations of the encystation and excystation processes in Diophrys oligothrix Borror, 1965 (Protista, Ciliophora)
    Zhi‐Wei Gong, Xin‐Peng Fan, Rui Ma, Bing Ni
    Journal of Morphology.2018; 279(10): 1397.     CrossRef
Research Support, Non-U.S. Gov'ts
Pregnancy - associated human listeriosis: Virulence and genotypic analysis of Listeria monocytogenes from clinical samples
Dharmendra Kumar Soni , Durg Vijai Singh , Suresh Kumar Dubey
J. Microbiol. 2015;53(9):653-660.   Published online August 1, 2015
DOI: https://doi.org/10.1007/s12275-015-5243-9
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AbstractAbstract
Listeria monocytogenes, a life-threatening pathogen, poses severe risk during pregnancy, may cause abortion, fetal death or neonatal morbidity in terms of septicemia and meningitis. The present study aimed at characterizing L. monocytogenes isolated from pregnant women based on serotyping, antibiotic susceptibility, virulence genes, in vivo pathogenicity test and ERIC- and REP-PCR fingerprint analyses. The results revealed that out of 3700 human clinical samples, a total of 30 (0.81%) isolates [12 (0.80%) from placental bit (1500), 18 (0.81%) from vaginal swab (2200)] were positive for L. monocytogenes. All the isolates belonged to serogroup 4b, and were + ve for virulence genes tested i.e. inlA, inlC, inlJ, plcA, prfA, actA, hlyA, and iap. Based on the mice inoculation tests, 20 isolates showed 100% and 4 isolates 60% relative virulence while 6 isolates were non-pathogenic. Moreover, 2 and 10 isolates were resistant to ciprofloxacin and cefoxitin, respectively, while the rest susceptible to other antibiotics used in this study. ERIC- and REP-PCR collectively depicted that the isolates from placental bit and vaginal swab had distinct PCR fingerprints except a few isolates with identical patterns. This study demonstrates prevalence of pathogenic strains mostly resistant to cefoxitin and/or ciprofloxacin. The results indicate the importance of isolating and characterizing the pathogen from human clinical samples as the pre-requisite for accurate epidemiological investigations.

Citations

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  • A comparative study on the occurrence, genetic characteristics, and factors associated with the distribution of Listeria species on cattle farms and beef abattoirs in Gauteng Province, South Africa
    J. Gana, N. Gcebe, R. Moerane, Y. B. Ngoshe, T. Tshuma, K. Moabelo, A. A. Adesiyun
    Tropical Animal Health and Production.2024;[Epub]     CrossRef
  • Clinical and Genomic Characteristics of a Clinical Listeria Monocytogenes ST120 Isolate Recovered from a Pregnant Woman
    Jingrui Zhang, Zengbin Liu, Zhirong Li, Caihong Xu, Hongbin Wang, Rugang Yang, Li Liu
    Infection and Drug Resistance.2024; Volume 17: 229.     CrossRef
  • Systematic review ofListeria monocytogenesfrom food and clinical samples in Chinese mainland from 2010 to 2019
    Ying Cheng, Qingli Dong, Yangtai Liu, Hong Liu, Hongzhi Zhang, Xiang Wang
    Food Quality and Safety.2022;[Epub]     CrossRef
  • Ecology of Listeria monocytogenes and Listeria species in India: the occurrence, resistance to biocides, genomic landscape and biocontrol
    Sukhadeo Baliram Barbuddhe, Deepak Bhiwa Rawool, Swapnil Prakash Doijad, Jess Vergis, Satyaveer Singh Malik, Trinad Chakraborty
    Environmental Microbiology.2022; 24(6): 2759.     CrossRef
  • Isolation and characterization of Listeria monocytogenes among women attending Jimma University medical center, Southwest Ethiopia
    Lencho Girma, Alene Geteneh, Demisew Amenu, Tesfaye Kassa
    BMC Infectious Diseases.2021;[Epub]     CrossRef
  • An Update Review on Listeria Infection in Pregnancy
    Zhaoyun Wang, Xiaojing Tao, Shan Liu, Yutong Zhao, Xiuhua Yang
    Infection and Drug Resistance.2021; Volume 14: 1967.     CrossRef
  • Evaluation of MALDI–TOF MS as a tool for detection of Listeria spp. directly from selective enrichment broth from food and stool samples
    Thais Martins Campos Araújo, Rodrigo de Castro Lisbôa Pereira, Isabelle Geoffroy Ribeiro Freitag, Leonardo Alves Rusak, Larissa Alvarenga Batista Botelho, Ernesto Hofer, Cristina Barroso Hofer, Deyse Christina Vallim
    Journal of Microbiological Methods.2020; 173: 105936.     CrossRef
  • Listeria monocytogenes detected in vaginal self-samples of 2 women after spontaneous miscarriage, Senegal, West Africa
    Ndeye Safietou Fall, Mariema Sarr, Nafissatou Diagne, Hubert Bassène, Cheikh Sokhna, Jean-Christophe Lagier, Didier Raoult
    European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(2): 393.     CrossRef
  • Characterisation of Listeria monocytogenes from Food and Human Clinical Samples at Duhok, Kurdistan Region of Iraq
    Azad Mohammed Taher Al-Brefkani, Ismaeil Mohammed Abdulkahar Mammani
    Journal of Pure and Applied Microbiology.2019; 13(4): 2215.     CrossRef
  • Pregnancy-related listeriosis: frequency and genotypic characteristics of L. monocytogenes from human specimens in Kerman, Iran
    Zahra Zahirnia, Shahla Mansouri, Fereshteh Saffari
    Wiener Medizinische Wochenschrift.2019; 169(9-10): 226.     CrossRef
  • Effect of free and nano-encapsulated curcumin on treatment and energetic metabolism of gerbils infected by Listeria monocytogenes
    Antonise M. Jaguezeski, Carine F. Souza, Gessica Perin, João H. Reis, Teane M.A. Gomes, Matheus D. Baldissera, Rodrigo A. Vaucher, Cinthia M. de Andrade, Lenita M. Stefani, Samanta S. Gundel, Aline F. Ourique, Aleksandro S. Da Silva
    Microbial Pathogenesis.2019; 134: 103564.     CrossRef
  • Retrospective Study of Listeria monocytogenes Isolated in the Territory of Inner Eurasia from 1947 to 1999
    Ekaterina K. Psareva, Irina Yu. Egorova, Elena A. Liskova, Irina V. Razheva, Nadezda A. Gladkova, Elena V. Sokolova, Eugene A. Potemkin, Pavel A. Zhurilov, Tatyana V. Mikhaleva, Andrei A. Blokhin, Yaroslava M. Chalenko, Denis V. Kolbasov, Svetlana A. Ermo
    Pathogens.2019; 8(4): 184.     CrossRef
  • Listeriolysin S may inhibit the anti-listerial properties of Lactobacillus plantarum
    Rokhsareh Mohammadzadeh, Azadeh Azadegan, Behrooz Sadeghi Kalani
    Microbial Pathogenesis.2019; 137: 103744.     CrossRef
  • Determination of Dominant Serovars and Molecular Analysis of hly and iap Genes Related to Listeria monocytogenes Strains Isolated from Spontaneous Human Abortions in Tehran
    Maryam Rezaei, Nadia Kazemipour, Jalil Vandyousefi, Farokh Rokhbakhshzamin, Gholamreza Irajian
    Iranian Journal of Medical Microbiology.2019; 13(2): 102.     CrossRef
  • Clinical management of women with listeriosis risk during pregnancy: a review of national guidelines
    Lisa Pucci, Mario Massacesi, Giuseppina Liuzzi
    Expert Review of Anti-infective Therapy.2018; 16(1): 13.     CrossRef
  • Listeriosis in pregnancy: under-diagnosis despite over-treatment
    Y Fouks, S Amit, A Many, A Haham, D Mandel, S Shinar
    Journal of Perinatology.2018; 38(1): 26.     CrossRef
  • Biosensor for the detection of Listeria monocytogenes: emerging trends
    Dharmendra Kumar Soni, Rafiq Ahmad, Suresh Kumar Dubey
    Critical Reviews in Microbiology.2018; 44(5): 590.     CrossRef
  • Comparative whole genome analysis of Listeria monocytogenes 4b strains reveals least genome diversification irrespective of their niche specificity
    Dharmendra K. Soni, Arpita Ghosh, Surendra K. Chikara, Krishna M. Singh, Chaitanya G. Joshi, Suresh K. Dubey
    Gene Reports.2017; 8: 61.     CrossRef
  • Progesterone impairs antigen-non-specific immune protection by CD8 T memory cells via interferon-γ gene hypermethylation
    Yushi Yao, Hui Li, Jie Ding, Yixin Xia, Lei Wang, Gongyi Zhang
    PLOS Pathogens.2017; 13(11): e1006736.     CrossRef
  • Prevalence and molecular characterization of Listeria spp. and Listeria monocytogenes isolated from fish, shrimp, and cooked ready-to-eat (RTE) aquatic products in Iran
    Esmail Abdollahzadeh, Seyed Mahdi Ojagh, Hedayat Hosseini, Gholamreza Irajian, Ezzat Allah Ghaemi
    LWT.2016; 73: 205.     CrossRef
  • Antimicrobial Hyaluronic Acid–Cefoxitin Sodium Thin Films Produced by Electrospraying
    Jayesh J. Ahire, Leon M. T. Dicks
    Current Microbiology.2016; 73(2): 236.     CrossRef
Improved pipeline for reducing erroneous identification by 16S rRNA sequences using the Illumina MiSeq platform
Yoon-Seong Jeon , Sang-Cheol Park , Jeongmin Lim , Jongsik Chun , Bong-Soo Kim
J. Microbiol. 2015;53(1):60-69.   Published online January 4, 2015
DOI: https://doi.org/10.1007/s12275-015-4601-y
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AbstractAbstract
The cost of DNA sequencing has decreased due to advancements in Next Generation Sequencing. The number of sequences obtained from the Illumina platform is large, use of this platform can reduce costs more than the 454 pyrosequencer. However, the Illumina platform has other challenges, including bioinformatics analysis of large numbers of sequences and the need to reduce erroneous nucleotides generated at the 3􍿁-ends of the sequences. These erroneous sequences can lead to errors in analysis of microbial communities. Therefore, correction of these erroneous sequences is necessary for accurate taxonomic identification. Several studies that have used the Illumina platform to perform metagenomic analyses proposed curating pipelines to increase accuracy. In this study, we evaluated the likelihood of obtaining an erroneous microbial composition using the MiSeq 250 bp paired sequence platform and improved the pipeline to reduce erroneous identifications. We compared different sequencing conditions by varying the percentage of control phiX added, the concentration of the sequencing library, and the 16S rRNA gene target region using a mock community sample composed of known sequences. Our recommended
method
corrected erroneous nucleotides and improved identification accuracy. Overall, 99.5% of the total reads shared 95% similarity with the corresponding template sequences and 93.6% of the total reads shared over 97% similarity. This indicated that the MiSeq platform can be used to analyze microbial communities at the genus level with high accuracy. The improved analysis method recommended in this study can be applied to amplicon studies in various environments using high-throughput reads generated on the MiSeq platform.

Citations

Citations to this article as recorded by  
  • Diversity analysis of the yeast and fungal community structure in Kazak cheese from the Yili Pastoral area in Xinjiang
    Yuhui Li, Chunyan Wang, Jungang Wang
    International Dairy Journal.2023; 143: 105672.     CrossRef
  • Effects of lactic acid bacteria fermented feed and three types of lactic acid bacteria (L. plantarum, L. acidophilus, B. animalis) on intestinal microbiota and T cell polarization (Th1, Th2, Th17, Treg) in the intestinal lymph nodes and spleens of rats
    Da Yoon Yu, Sang-Hyon Oh, In Sung Kim, Gwang Il Kim, Jeong A Kim, Yang Soo Moon, Jae Cheol Jang, Sang Suk Lee, Jong Hyun Jung, Hwa Chun Park, Kwang Keun Cho
    Animal Bioscience.2023; 36(1): 156.     CrossRef
  • Soil microbial community and their functional genes during grassland restoration
    Jiaojiao Liao, Yanxing Dou, Xuan Yang, Shaoshan An
    Journal of Environmental Management.2023; 325: 116488.     CrossRef
  • Remediation of fomesafen contaminated soil by Bacillus sp. Za: Degradation pathway, community structure and bioenhanced remediation
    Guoqiang Zhao, Jing Zhou, Yanning Tian, Qifeng Chen, Dongmei Mao, Jianchun Zhu, Xing Huang
    Environmental Pollution.2023; 336: 122460.     CrossRef
  • Inactivation of Antibiotic-Resistant Bacteria in Wastewater by Ozone-Based Advanced Water Treatment Processes
    Takashi Azuma, Masaru Usui, Tetsuya Hayashi
    Antibiotics.2022; 11(2): 210.     CrossRef
  • LotuS2: an ultrafast and highly accurate tool for amplicon sequencing analysis
    Ezgi Özkurt, Joachim Fritscher, Nicola Soranzo, Duncan Y. K. Ng, Robert P. Davey, Mohammad Bahram, Falk Hildebrand
    Microbiome.2022;[Epub]     CrossRef
  • Intestinal microbial composition changes induced by Lactobacillus plantarum GBL 16, 17 fermented feed and intestinal immune homeostasis regulation in pigs
    Da Yoon Yu, Sang-Hyon Oh, In Sung Kim, Gwang Il Kim, Jeong A Kim, Yang Soo Moon, Jae Cheol Jang, Sang Suk Lee, Jong Hyun Jung, Jun Park, Kwang Keun Cho
    Journal of Animal Science and Technology.2022; 64(6): 1184.     CrossRef
  • Investigating the core microbiota and its influencing factors in traditional Chinese pickles
    Feiyu An, Huijun Sun, Junrui Wu, Chunyan Zhao, Tong Li, Heting Huang, Qiang Fang, Endong Mu, Rina Wu
    Food Research International.2021; 147: 110543.     CrossRef
  • Soil remediation approach and bacterial community structure in a long-term contaminated soil by a mining spill (Aznalcóllar, Spain)
    M. Paniagua-López, M. Vela-Cano, D. Correa-Galeote, F. Martín-Peinado, F.J. Martínez Garzón, C. Pozo, J. González-López, M. Sierra Aragón
    Science of The Total Environment.2021; 777: 145128.     CrossRef
  • Effects of natural sunlight on antimicrobial-resistant bacteria (AMRB) and antimicrobial-susceptible bacteria (AMSB) in wastewater and river water
    Takashi Azuma, Tetsuya Hayashi
    Science of The Total Environment.2021; 766: 142568.     CrossRef
  • On-site chlorination responsible for effective disinfection of wastewater from hospital
    Takashi Azuma, Tetsuya Hayashi
    Science of The Total Environment.2021; 776: 145951.     CrossRef
  • Disinfection of Antibiotic-resistant Bacteria in Sewage and Hospital Effluent by Ozonation
    Takashi Azuma, Tetsuya Hayashi
    Ozone: Science & Engineering.2021; 43(5): 413.     CrossRef
  • Analysis of bacterial diversity and biogenic amines content during fermentation of farmhouse sauce from Northeast China
    Yu Hao, Bo Sun
    Food Control.2020; 108: 106861.     CrossRef
  • Performance of enhanced anaerobic digestion with different pyrolysis biochars and microbial communities
    Ruixia Shen, Yong Jing, Jing Feng, Juan Luo, Jiadong Yu, Lixin Zhao
    Bioresource Technology.2020; 296: 122354.     CrossRef
  • Astrovirus infects actively secreting goblet cells and alters the gut mucus barrier
    Valerie Cortez, David F. Boyd, Jeremy Chase Crawford, Bridgett Sharp, Brandi Livingston, Hannah M. Rowe, Amy Davis, Ramzi Alsallaq, Camenzind G. Robinson, Peter Vogel, Jason W. Rosch, Elisa Margolis, Paul G. Thomas, Stacey Schultz-Cherry
    Nature Communications.2020;[Epub]     CrossRef
  • Metagenomic analysis of bacterial community structure and functions during the fermentation of da-jiang, a Chinese traditional fermented food
    Mengxi Xie, Feiyu An, Yue Zhao, Rina Wu, Junrui Wu
    LWT.2020; 129: 109450.     CrossRef
  • Relationship between nasopharyngeal microbiota and patient’s susceptibility to viral infection
    Grégory Dubourg, Sophie Edouard, Didier Raoult
    Expert Review of Anti-infective Therapy.2019; 17(6): 437.     CrossRef
  • Structure and Diversity of Soil Bacterial Communities in Offshore Islands
    Yu-Te Lin, Yu-Fei Lin, Isheng J. Tsai, Ed-Haun Chang, Shih-Hao Jien, Yen-Ju Lin, Chih-Yu Chiu
    Scientific Reports.2019;[Epub]     CrossRef
  • Meta‐omics reveal microbial assortments and key enzymes in bean sauce mash, a traditional fermented soybean product
    Mengxi Xie, Feiyu An, Junrui Wu, Yiming Liu, Haishu Shi, Rina Wu
    Journal of the Science of Food and Agriculture.2019; 99(14): 6522.     CrossRef
  • Insights into evolutionary trends in molecular biology tools in microbial screening for biohydrogen production through dark fermentation
    Gopalakrishnan Kumar, Si-Kyung Cho, Periyasamy Sivagurunathan, Parthiban Anburajan, Durga Madhab Mahapatra, Jeong-Hoon Park, Arivalagan Pugazhendhi
    International Journal of Hydrogen Energy.2018; 43(43): 19885.     CrossRef
  • Analyses of microbial community of naturally homemade soybean pastes in Liaoning Province of China by Illumina Miseq Sequencing
    Xiaodong Sun, Guozhong Lyu, Yushi Luan, Zhihui Zhao, Hong Yang, Dan Su
    Food Research International.2018; 111: 50.     CrossRef
  • Effects of Rubus coreanus byproducts on intestinal microbiota and the immune modulation
    Da Yoon Yu, Sung Hak Kim, Jeong A Kim, In Sung Kim, Yang Soo Moon, Sang Suk Lee, Hwa Chun Park, Jong Hyun Jung, Yi Hyung Chung, Dae Keun Shin, Ki Chang Nam, In Soon Choi, Kwang Keun Cho
    Asian-Australasian Journal of Animal Sciences.2018; 31(3): 429.     CrossRef
  • Sequencing Batch Reactor and Bacterial Community in Aerobic Granular Sludge for Wastewater Treatment of Noodle-Manufacturing Sector
    Tang Chinh, Phung Hieu, Bui Cuong, Nguyen Linh, Nguyen Lan, Nguyen Nguyen, Nguyen Hung, Le Hien
    Applied Sciences.2018; 8(4): 509.     CrossRef
  • An improved metagenomic strategy reveals an unprecedentedly high level of intragenomic polymorphism of ribosomal DNA in three species of Camellia
    Yang Shao, Min Zhang, Ying Xu, Yong‐Qing Zhu, Takahiro Yonezawa, Yu‐Guo Wang, Zhi‐Ping Song, Wen‐Ju Zhang
    Journal of Systematics and Evolution.2018; 56(3): 250.     CrossRef
  • Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber Apostichopus japonicus in two habitats
    Qi Wang, Xiumei Zhang, Muyan Chen, Wentao Li, Peidong Zhang
    Journal of Oceanology and Limnology.2018; 36(3): 990.     CrossRef
  • DNA Sequencing as a Tool to Monitor Marine Ecological Status
    Kelly D. Goodwin, Luke R. Thompson, Bernardo Duarte, Tim Kahlke, Andrew R. Thompson, João C. Marques, Isabel Caçador
    Frontiers in Marine Science.2017;[Epub]     CrossRef
  • Differences in root-associated bacterial communities among fine root branching orders of poplar (Populus × euramericana (Dode) Guinier.)
    Qitong Wang, Nian Wang, Yanping Wang, Qingkui Wang, Baoli Duan
    Plant and Soil.2017; 421(1-2): 123.     CrossRef
  • Advanced nitrogen removal from the biological secondary effluent of dyeing wastewater via a biological–ferric–carbon nitrification and denitrification process
    Hong Chen, Gang Xue, Mengran Jiang, Yuying Cheng
    RSC Advances.2016; 6(108): 106951.     CrossRef
  • Microbial electrolysis cell to treat hydrothermal liquefied wastewater from cornstalk and recover hydrogen: Degradation of organic compounds and characterization of microbial community
    Ruixia Shen, Zhidan Liu, Yanhong He, Yuanhui Zhang, Jianwen Lu, Zhangbing Zhu, Buchun Si, Chong Zhang, Xin-Hui Xing
    International Journal of Hydrogen Energy.2016; 41(7): 4132.     CrossRef
  • DAMe: a toolkit for the initial processing of datasets with PCR replicates of double-tagged amplicons for DNA metabarcoding analyses
    Marie Lisandra Zepeda-Mendoza, Kristine Bohmann, Aldo Carmona Baez, M. Thomas P. Gilbert
    BMC Research Notes.2016;[Epub]     CrossRef
  • Studying long 16S rDNA sequences with ultrafast-metagenomic sequence classification using exact alignments (Kraken)
    Fabiola Valenzuela-González, Marcel Martínez-Porchas, Enrique Villalpando-Canchola, Francisco Vargas-Albores
    Journal of Microbiological Methods.2016; 122: 38.     CrossRef
  • Adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose
    Jungseok Lee, Jack N. Saddler, Youngsoon Um, Han Min Woo
    Microbial Cell Factories.2016;[Epub]     CrossRef
  • Bacteria Associated WithPiezodorus guildinii(Hemiptera: Pentatomidae), With Special Reference to Those Transmitted by Feeding
    Claudia Husseneder, Jong-Seok Park, Andrea Howells, Chinmay V. Tikhe, Jeffrey A. Davis
    Environmental Entomology.2016; : nvw112.     CrossRef
  • Fecal Microbiota Analysis: An Overview of Sample Collection Methods and Sequencing Strategies
    Vincent Thomas, James Clark, Joël Doré
    Future Microbiology.2015; 10(9): 1485.     CrossRef
Performance of a real-time PCR assay for the rapid identification of Mycobacterium species
Hye-young Wang , Hyunjung Kim , Sunghyun Kim , Do-kyoon Kim , Sang-Nae Cho , Hyeyoung Lee
J. Microbiol. 2015;53(1):38-46.   Published online January 4, 2015
DOI: https://doi.org/10.1007/s12275-015-4495-8
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AbstractAbstract
Mycobacteria cause a variety of illnesses that differ in severity and public health implications. The differentiation of Mycobacterium tuberculosis (MTB) from nontuberculous mycobacteria (NTM) is of primary importance for infection control and choice of antimicrobial therapy. The diagnosis of diseases caused by NTM is difficult because NTM species are prevalent in the environment and because they have fastidious properties. In the present study, we evaluated 279 clinical isolates grown in liquid culture provided by The Catholic University of Korea, St. Vincent’s Hospital using real-time PCR based on mycobacterial rpoB gene sequences. The positive rate of real-time PCR assay accurately discriminated 100% (195/195) and 100% (84/84) between MTB and NTM species. Comparison of isolates identified using the MolecuTech REBA Myco-ID? and Real Myco-ID? were completely concordant except for two samples. Two cases that were identified as mixed infection (M. intracellulare-M. massiliense and M. avium-M. massiliense co-infection) by PCRREBA assay were only detected using M. abscessus-specific probes by Real Myco-ID?. Among a total of 84 cases, the most frequently identified NTM species were M. intracellulare (n=38, 45.2%), M. avium (n=18, 23.7%), M. massiliense (n=10, 13.2%), M. fortuitum (n=5, 6%), M. abscessus (n=3, 3.9%), M. gordonae (n=3, 3.9%), M. kansasii (n=2, 2.4%), M. mucogenicum (n=2, 2.4%), and M. chelonae (n= 1, 1.2%). Real Myco-ID? is an efficient tool for the rapid detection of NTM species as well as MTB and sensitive and specific and comparable to conventional methods.

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    Guocan Yu, Yanqin Shen, Liwei Yao, Xudong Xu
    Infection and Drug Resistance.2024; Volume 17: 673.     CrossRef
  • Preclinical murine models for the testing of antimicrobials against Mycobacterium abscessus pulmonary infections: Current practices and recommendations
    Véronique Dartois, Tracey L. Bonfield, Jim P. Boyce, Charles L. Daley, Thomas Dick, Mercedes Gonzalez-Juarrero, Shashank Gupta, Igor Kramnik, Gyanu Lamichhane, Barbara E. Laughon, Nicola I. Lorè, Kenneth C. Malcolm, Kenneth N. Olivier, Katherine L. Tuggle
    Tuberculosis.2024; 147: 102503.     CrossRef
  • Durlobactam to boost the clinical utility of standard of care β-lactams against Mycobacterium abscessus lung disease
    Dereje A. Negatu, Wassihun Wedajo Aragaw, Tewodros T. Gebresilase, Sindhuja Paruchuri, Firat Kaya, Sung Jae Shin, Peter Sander, Véronique Dartois, Thomas Dick, Jared A. Silverman
    Antimicrobial Agents and Chemotherapy.2024;[Epub]     CrossRef
  • Epidemiology and laboratory detection of non-tuberculous mycobacteria
    Nuo Xu, Lihong Li, Shenghai Wu
    Heliyon.2024; 10(15): e35311.     CrossRef
  • Molecular identification of non-tuberculous mycobacterial species isolated from extrapulmonary samples using real-time PCR and rpoB sequence analysis
    Mohammad Hashemzadeh, Aram Asarehzadegan Dezfuli, Azar Dokht Khosravi, Maryam Moradi Bandbal, Atousa Ghorbani, Mahtab Hamed, Soolmaz Khandan Dezfuli
    AMB Express.2023;[Epub]     CrossRef
  • TO ANALYZE THE TB-PCR POSITIVITY RATE USING REAL-TIME PCR FOR EARLY DETECTION OF TUBERCULOSIS
    DEEPAK SAWANT, LOKHANDE CD, SHARMA RK, CHOUGULE RA
    Asian Journal of Pharmaceutical and Clinical Research.2023; : 167.     CrossRef
  • Factors Associated with the Performance of Direct PCR Detection of Mycobacteria in Clinical Specimens: Retrospective Real-world Data
    Chang-Hun Park
    Laboratory Medicine Online.2023; 13(3): 205.     CrossRef
  • Evaluation of a new assay for nontuberculous mycobacteria species identification in diagnostic material and cultures
    Tatiana Smirnova, Vera Ustinova, Sofya Andreevskaya, Elena Larionova, Ekaterina Kiseleva, Larisa Chernousova, Dmitry Varlamov, Dmitry Sochivko, Atadzhan Ergeshov
    Tuberculosis.2021; 130: 102124.     CrossRef
  • Cas12a/Guide RNA-Based Platform for Rapid and Accurate Identification of Major Mycobacterium Species
    Guohui Xiao, Xing He, Su Zhang, Yaya Liu, Zhihang Liang, Houming Liu, Juanjuan Zhang, Min Ou, Shuhao Cai, Wenjie Lai, Tianyu Zhang, Lili Ren, Guoliang Zhang, Yi-Wei Tang
    Journal of Clinical Microbiology.2020;[Epub]     CrossRef
  • Diagnostic Performance of the GENEDIA MTB/NTM Detection Kit for Detecting Mycobacterium tuberculosis and Nontuberculous Mycobacteria With Sputum Specimens
    Sunghwan Shin, In Young Yoo, Hyang Jin Shim, On Kyun Kang, Byung Woo Jhun, Won-Jung Koh, Hee Jae Huh, Nam Yong Lee
    Annals of Laboratory Medicine.2020; 40(2): 169.     CrossRef
  • Diagnostic performance of real time PCR and MALDI-TOF in the detection of nontuberculous mycobacteria from clinical isolates
    Ellappan Kalaiarasan, Kalpana Thangavelu, Krishnakumariamma Krishnapriya, Muthaiah Muthuraj, Maria Jose, Noyal Mariya Joseph
    Tuberculosis.2020; 125: 101988.     CrossRef
  • Comparison of the Three Molecular Diagnostic Assays for Molecular Identification ofMycobacterium tuberculosisand Nontuberculous Mycobacteria Species in Sputum Samples
    Jinyoung Bae, Sung-Bae Park, Ji-Hoi Kim, Mi Ran Kang, Kyung Eun Lee, Sunghyun Kim, Hyunwoo Jin
    Biomedical Science Letters.2020; 26(3): 170.     CrossRef
  • Rapid Identification of Clinically Relevant Mycobacterium Species by Multicolor Melting Curve Analysis
    Ye Xu, Bin Liang, Chen Du, Xueshan Tian, Xingshan Cai, Yanjie Hou, Hui Li, Rongrong Zheng, Junlian Li, Yuqin Liu, Kaili Wang, Muhammad Ammar Athar, Yaoju Tan, Qingge Li, Melissa B. Miller
    Journal of Clinical Microbiology.2019;[Epub]     CrossRef
  • Mycobacterium abscessus Complex Cutaneous Infection
    Ruben Porudominsky, Eduardo H. Gotuzzo
    Current Tropical Medicine Reports.2018; 5(3): 170.     CrossRef
  • The assessment of host and bacterial proteins in sputum from active pulmonary tuberculosis
    Hsin-Chih Lai, Yu-Tze Horng, Pen-Fang Yeh, Jann-Yuan Wang, Chin-Chung Shu, Chia-Chen Lu, Jang-Jih Lu, Jen-Jyh Lee, Po-Chi Soo
    Journal of Microbiology.2016; 54(11): 761.     CrossRef
  • Efectos adversos de terapia inmunosupresora en paciente reumatológico: infección por micobacterias no tuberculosas
    Jean Sebastian Hurtado Hurtado
    Reumatología Clínica.2016; 12(2): 118.     CrossRef
  • Adverse Effects of Immunosuppressive Therapy in Rheumatic Patients: Non-tuberculous Mycobacterial Infection
    Jean Sebastian Hurtado Hurtado
    Reumatología Clínica (English Edition).2016; 12(2): 118.     CrossRef
  • Nontuberculous Mycobacterial Lung Disease Caused byMycobacterium shinjukuense: The First Reported Case in Korea
    Seong Mi Moon, Su-Young Kim, Myung Jin Chung, Seung Heon Lee, Sung Jae Shin, Won-Jung Koh
    Tuberculosis and Respiratory Diseases.2015; 78(4): 416.     CrossRef
Characterization of Thermostable Deblocking Aminopeptidases of Archaeon Thermococcus onnurineus NA1 by Proteomic and Biochemical Approaches
Yeol Gyun Lee , Sun-Hee Leem , Young-Ho Chung , Seung Il Kim
J. Microbiol. 2012;50(5):792-797.   Published online November 4, 2012
DOI: https://doi.org/10.1007/s12275-012-2461-2
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AbstractAbstract
Thermococcus onnurineus NA1 is a hyperthermophilic archaeon that grows optimally at >80°C. The deblocking aminopeptidase (DAP) (TNA1-DAP1) encoded in Ton_1032 of T. onnurineus NA1 is considered a major DAP. However, four genes encoding putative DAP have been identified from a genomic analysis of T. onnurineus NA1. A proteomic analysis revealed that all four DAPs were differentially induced in YPS culture medium and, particularly, two DAPs (TNA1-DAP1 and TNA1-DAP2) were dominantly expressed in T. onnurineus NA1. The biochemical properties and enzyme activity of DAPs induced in an E. coli expression system suggested that the two major DAPs play complementary roles in T. onnurineus NA1.
NOTE] A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi
Yuanyuan Chen , Bernard A. Prior , Guiyang Shi , Zhengxiang Wang
J. Microbiol. 2011;49(4):675-679.   Published online September 2, 2011
DOI: https://doi.org/10.1007/s12275-011-0525-3
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AbstractAbstract
In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates.
Journal Articles
NOTE] Taxonomy of Eurotium Species Isolated from Meju
Seung-Beom Hong , Dae-Ho Kim , Mina Lee , Seong-Yeol Baek , Soon-Wo Kwon , Robert A. Samson
J. Microbiol. 2011;49(4):669-674.   Published online September 2, 2011
DOI: https://doi.org/10.1007/s12275-011-0376-y
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  • 30 Scopus
AbstractAbstract
Eurotium strains were isolated from 77 loaves of meju (dried fermented soybeans), in various regions of Korea from 2008 to 2010. Morphological characteristics and DNA sequences of β-tubulin were examined. They were identified as Eurotium amstelodami, E. chevalieri, E. herbariorum, E. repens, E. rubrum, and E. tonophilum. Of these species, E. chevalieri and E. tonophilum had not been previously reported in association with meju. E. chevalieri and E. repens were the species isolated most frequently. This paper summarizes the morphological characteristics of six Eurotium species and provides key to identify the species from meju.
Re-identification of Aspergillus fumigatus sensu lato Based on a New Concept of Species Delimitation
Seung-Beom Hong , Dae-Ho Kim , In-Cheol Park , Young-Joon Choi , Hyeon-Dong Shin , Robert Samson
J. Microbiol. 2010;48(5):607-615.   Published online November 3, 2010
DOI: https://doi.org/10.1007/s12275-010-0084-z
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  • 16 Scopus
AbstractAbstract
The species concept of Aspergillus fumigatus sensu stricto has recently been defined by polyphasic taxonomy. Based on the new concept of species delimitations, 146 worldwide strains of Aspergillus fumigatus sensu lato were re-identified. Of those 146 strains, 140 (95.8%) could be identified as A. fumigatus sensu stricto, 3 (2.1%) as A. lentulus, and the remaining 3 strains as A. viridinutans complex, Neosartorya udagawae, and N. cf. nishimurae. Of 98 clinical strains, only 1 from dolphin nostril was identified as A. lentulus and not A. fumigatus sensu stricto. Random amplification of polymorphic DNA-polymerase chain reaction (RAPD-PCR) with primers PELF and URP1F produced nearly the same band patterns among 136 strains of A. fumigatus sensu stricto while discriminated the species from its related species. We also discussed about identification of several atypical A. fumigatus strains from clinical environments.
Development of a Method Based on Surface Enhanced Laser Desorption and Ionization Time of Flight Mass Spectrometry for Rapid Identification of Klebsiella pneumoniae
Daiwen Xiao , Yongchang Yang , Hua Liu , Hua Yu , Yingjun Yan , Wenfang Huang , Wei Jiang , Weijin Liao , Qi Hu , Bo Huang
J. Microbiol. 2009;47(5):646-650.   Published online October 24, 2009
DOI: https://doi.org/10.1007/s12275-009-0092-z
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  • 4 Scopus
AbstractAbstract
A method based on surface enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF MS) was developed for the rapid identification of Klebsiella pneumoniae by directly applying bacterial colonies without further protein extraction. A total of 40 K. pneumoniae and 114 other related microorganisms isolated clinically were analyzed by SELDI-TOF MS. An identification model for K. pneumoniae was established by artificial neural networks (ANNs) with classification accuracy of 100%. The model was blindly tested with 43 K. pneumoniae and 53 control bacteria again. The results showed that the model was successful with accuracy of 96.9%, sensitivity of 100% and specificity of 94.3%. This strategy is potential for rapid identification of K. pneumoniae.
Research Support, Non-U.S. Gov'ts
High Infectivity of an Endoparasitic Fungus Strain, Esteya vermicola, against Nematodes
Chun Yan Wang , Zhe Ming Fang , Bai Shen Sun , Li Juan Gu , Ke Qin Zhang , Chang-Keun Sung
J. Microbiol. 2008;46(4):380-389.   Published online August 31, 2008
DOI: https://doi.org/10.1007/s12275-007-0122-7
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AbstractAbstract
Esteya vermicola, as the first recorded endoparasitic fungus of pinewood nematodes, exhibits great potential as a biological agent against nematodes. However, only two strains of this species have been described so far. In this study, we identified a novel endoparasitic fungal strain, CNU 120806, isolated from infected nematodes in forest soil samples during a survey of nematophagous fungi in Korea. This strain showed similar morphological characteristics and infection mode with the two previously described strains of E. vermicola. All strains are characterized by the ability to produce two types of conidiogenous cells and conidia, and to parasitize nematodes with lunate adhesive conidia. Moreover, the CNU 120806 strain showed 100% identity with E. vermicola CBS 115803 when their partial sequences of 28S rRNA gene were compared. Molecular phylogenetic analysis further identified CNU 120806 as a strain of E. vermicola, by clustering CNU 120806 and E. vermicola CBS 115803 into a single subclade. Culture medium influenced the proportion of dimorphic CNU 120806 conidia, and further changed the adhesive and mortality rates of nematodes. The CNU 120806 strain exhibits high infection activity against nematodes on nutrient-rich PDA medium. Almost all tested nematodes were killed within 8~10 days after inoculation. This study provides justification for further research of E. vermicola, and the application and formulation of this fungus as a bio-control agent against nematodes.

Citations

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  • Screening and Optimization of Solid-State Fermentation for Esteya vermicola, an Entomopathogenic Fungus Against the Major Forest Pest Pine Wood Nematode
    Lanwen Zhang, Yongxia Li, Xiaojian Wen, Xuan Wang, Wei Zhang, Dongzhen Li, Yuqian Feng, Zhenkai Liu, Xingyao Zhang
    Microorganisms.2025; 13(2): 434.     CrossRef
  • Using Bursaphelenchus mucronatus to demonstrate the potential nematicidal effect of Beauveria bassiana on pine wood nematode (Bursaphelenchus xylophilus) under in vivo conditions
    Tamara Sánchez-Gómez, Paula Zamora, Julio Javier Díez, Baudilio Herrero, Jorge Poveda, Jorge Martín-García
    Annals of Forest Science.2025;[Epub]     CrossRef
  • The essential role of arginine biosynthetic genes in lunate conidia formation, conidiation, mycelial growth, and virulence of nematophagous fungus, Esteya vermicolaCBS115803
    Chi Chen, Zhijuan Hu, Xinyao Zheng, Jingjie Yuan, Run Zou, Yilan Wang, Xuan Peng, Chengjian Xie
    Pest Management Science.2024; 80(2): 786.     CrossRef
  • Unraveling the interactions of Esteya vermicola, pinewood nematode, and pine hosts: Insights into population dynamics and molecular responses
    Haihua Wang, Yulou Sun, Can Yin, Jie Gao, Jingfei Hu, Chungkeun Sung, Chunyan Wang, Zhen Wang
    Chemosphere.2024; 363: 142948.     CrossRef
  • Nematicidal effect of Beauveria species and the mycotoxin beauvericin against pinewood nematode Bursaphelenchus xylophilus
    Tamara Sánchez-Gómez, Steven J. Harte, Paula Zamora, Matéo Bareyre, Julio Javier Díez, Baudilio Herrero, Jonathan Niño-Sánchez, Jorge Martín-García
    Frontiers in Forests and Global Change.2023;[Epub]     CrossRef
  • Isolation and identification of Esteya vermicola and its potential for controlling pinewood nematode
    Xuan Wang, Yong Xia Li, Zhen Kai Liu, Xiao Jian Wen, Ze Sang Zi, Yu Qian Feng, Wei Zhang, Dong Zhen Li, Xing Yao Zhang
    Forest Pathology.2022;[Epub]     CrossRef
  • Research Progress on Biocontrol of Pine Wilt Disease by Microorganisms
    Guiming Dou, Dong-Hui Yan
    Forests.2022; 13(7): 1047.     CrossRef
  • The Potential of Esteya spp. for the Biocontrol of the Pinewood Nematode, Bursaphelenchus xylophilus
    David Pires, Cláudia S. L. Vicente, Maria L. Inácio, Manuel Mota
    Microorganisms.2022; 10(1): 168.     CrossRef
  • Migration of pinewood nematode promotes the colonization of Esteya vermicola in pine tree
    Hai‐Hua Wang, Can Yin, Ran Tao, Jie Gao, Yu‐Lou Sun, Jeong‐Hee Cho, Zhen Wang, Yun‐Bo Wang, Chun‐Yan Wang, Chang‐Keun Sung, Maria L. Inácio
    Forest Pathology.2021;[Epub]     CrossRef
  • Esteya floridanum sp. nov.: An Ophiostomatalean Nematophagous Fungus and Its Potential to Control the Pine Wood Nematode
    You Li, Haiying Yu, João P. M. Araújo, Xinfeng Zhang, Yingchao Ji, Jiri Hulcr
    Phytopathology®.2021; 111(2): 304.     CrossRef
  • Effect of culture conditions on conidia production and enhancement of environmental stress resistance of Esteya vermicola in solid‐state fermentation
    Y. Zhu, Y. Mao, T. Ma, X. Wen
    Journal of Applied Microbiology.2021; 131(1): 404.     CrossRef
  • Insights into the Role of Fungi in Pine Wilt Disease
    Cláudia S. L. Vicente, Miguel Soares, Jorge M. S. Faria, Ana P. Ramos, Maria L. Inácio
    Journal of Fungi.2021; 7(9): 780.     CrossRef
  • Hypothesized mechanism of biocontrol against pine wilt disease by the nematophagous fungus Esteya vermicola
    Can Yin, Yunbo Wang, Yong-an Zhang, Haihua Wang, Beibei Duan, Ran Tao, Jie Gao, Chang-keun Sung
    European Journal of Plant Pathology.2020; 156(3): 811.     CrossRef
  • A short review of the pinewood nematode, Bursaphelenchus xylophilus
    Bit-Na Kim, Ji Hun Kim, Ji-Young Ahn, Sunchang Kim, Byung-Kwan Cho, Yang-Hoon Kim, Jiho Min
    Toxicology and Environmental Health Sciences.2020; 12(4): 297.     CrossRef
  • In vivo infection of Bursaphelenchus xylophilus by the fungus Esteya vermicola
    Hai‐Hua Wang, Yun‐Bo Wang, Can Yin, Jie Gao, Ran Tao, Yu‐Lou Sun, Chun‐Yan Wang, Zhen Wang, Yong‐Xia Li, Chang‐Keun Sung
    Pest Management Science.2020; 76(8): 2854.     CrossRef
  • A pine wood sample preparation method for high target and quality DNA extraction for detection of Esteya vermicola by PCR from living pine
    Can Yin, Yunbo Wang, Yongan Zhang, Haihua Wang, Ran Tao, Yu Li, Chang Keun Sung
    Journal of Basic Microbiology.2019; 59(4): 437.     CrossRef
  • Differential patterns of ophiostomatoid fungal communities associated with three sympatric Tomicus species infesting pines in south-western China, with a description of four new species
    Wang HuiMin, Zheng Wang, Fu Liu, Cheng Xu Wu, Su Fang Zhang, Xiang Bo Kong, Cony Decock, Quan Lu, Zhen Zhang
    MycoKeys.2019; 50: 93.     CrossRef
  • Signal pathways involved in microbe–nematode interactions provide new insights into the biocontrol of plant-parasitic nematodes
    Lian-Ming Liang, Cheng-Gang Zou, Jianping Xu, Ke-Qin Zhang
    Philosophical Transactions of the Royal Society B: Biological Sciences.2019; 374(1767): 20180317.     CrossRef
  • The complete mitochondrial genome of the Dutch elm disease fungus Ophiostoma novo-ulmi subsp. novo-ulmi
    Talal George Abboud, Abdullah Zubaer, Alvan Wai, Georg Hausner
    Canadian Journal of Microbiology.2018; 64(5): 339.     CrossRef
  • Using the nematophagous fungus Esteya vermicola to control the disastrous pine wilt disease
    Chun Yan Wang, Can Yin, Zhe Ming Fang, Zhen Wang, Yun Bo Wang, Jian Jie Xue, Li Juan Gu, Chang Keun Sung
    Biocontrol Science and Technology.2018; 28(3): 268.     CrossRef
  • Comparative genomic analyses reveal the features for adaptation to nematodes in fungi
    Ruizhen Wang, Leiming Dong, Ran He, Qinghua Wang, Yuequ Chen, Liangjian Qu, Yong-An Zhang
    DNA Research.2018; 25(3): 245.     CrossRef
  • Esteya vermicola Controls the Pinewood Nematode, Bursaphelenchus xylophilus, in Pine Seedlings
    Zhen Wang, Yongan Zhang, Chunyan Wang, Yunbo Wang, Chungkeun Sung
    Journal of Nematology.2017; 49(1): 86.     CrossRef
  • Esteya Vermicola, a Nematophagous Fungus Attacking the Pine Wood Nematode, Harbors a Bacterial Endosymbiont Affiliated with Gammaproteobacteria
    Ruizhen Wang, Leiming Dong, Yuequ Chen, Liangjian Qu, Qinghua Wang, Yongan Zhang
    Microbes and environments.2017; 32(3): 201.     CrossRef
  • The complete mitochondrial genome of nematophagous fungus Esteya vermicola
    Ruizhen Wang, Leiming Dong, Yuequ Chen, Liangjian Qu, Enjie Li, Qinghua Wang, Yongan Zhang
    Mitochondrial DNA Part B.2017; 2(1): 196.     CrossRef
  • Population dynamics of pinewood nematode and the endoparasitic fungusEsteya vermicola: interactions under experimental conditions
    Zhen Wang, Chunyan Wang, Changkeun Sung
    Biocontrol Science and Technology.2015; 25(11): 1299.     CrossRef
  • Efficacy of commercial wood preservatives against Bursaphelenchus xylophilus, a quarantine organism in Europe
    S. C. Arcos, L. Robertson, M. Conde, F. Llinares, A. Navas, M. T. Troya
    European Journal of Wood and Wood Products.2015; 73(5): 643.     CrossRef
  • The effects of fluctuating culture temperature on stress tolerance and antioxidase expression in Esteya vermicola
    Yun-bo Wang, Wen-xing Pang, Xiao-na Yv, Jing-jie Li, Yong-an Zhang, Chang-keun Sung
    Journal of Microbiology.2015; 53(2): 122.     CrossRef
  • Research advance on Esteya vermicola, a high potential biocontrol agent of pine wilt disease
    Wen Hui Chu, Qing Dou, Hong Long Chu, Hai Hua Wang, Chang Keun Sung, Chun Yan Wang
    Mycological Progress.2015;[Epub]     CrossRef
  • Cloning of a serine protease gene from the nematophagous fungus Esteya vermicola and expressed activity of the recombinant enzyme against Bursaphelenchus xylophilus
    Xuan Wang, Tinglong Guan, Long Zhang, Hongmei Li
    Nematology.2015; 17(9): 1071.     CrossRef
  • Eat or be eaten: fungus and nematode switch off as predator and prey
    E. Erin Morris, Ann E. Hajek
    Fungal Ecology.2014; 11: 114.     CrossRef
  • Optimization of Promoting Conidial Production of a Pinewood Nematode Biocontrol Fungus, Esteya vermicola Using Response Surface Methodology
    Jianjie Xue, Jingang Hou, Yongan Zhang, Yuzhu Wang, Mira Lee, Jiaojiao Yu, Changkeun Sung
    Current Microbiology.2014; 69(5): 745.     CrossRef
  • Morphological, molecular and biological characterization of Esteya vermicola, a nematophagous fungus isolated from intercepted wood packing materials exported from Brazil
    Xuan Wang, Tingting Wang, Jincheng Wang, Tinglong Guan, Hongmei Li
    Mycoscience.2014; 55(5): 367.     CrossRef
  • Rapid molecular detection ofEsteya vermicolabased on specific primers and the FTA-DNA extraction method
    Ke Wei, Qinghua Wang, Yuzhu Wang, Liangjian Qu, Yong-an Zhang
    Biocontrol Science and Technology.2014; 24(8): 872.     CrossRef
  • A Staining Method for Assessing the Viability of Esteya vermicola Conidia
    Yunbo Wang, NguyenTrong Thang, Zheng Li, Yongan Zhang, Jingjie Li, Jianjie Xue, Lijuan Gu, VuThuy Hong, Lee Mira, Changkeun Sung
    Current Microbiology.2014; 69(1): 53.     CrossRef
  • Optimization of storage condition for maintaining long-term viability of nematophagous fungus Esteya vermicola as biocontrol agent against pinewood nematode
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Development of Strain-specific PCR Primers Based on a DNA Probe Fu12 for the Identification of Fusobacterium nucleatum subsp. nucleatum ATCC 25586^T
Hwa-Sook Kim , Soo Keun Song , So Young Yoo , Dong Chun Jin , Hwan Seon Shin , Chae Kwang Lim , Myung-Soo Kim , Jin-Soo Kim , Son-Jin Choe , Joong-Ki Kook
J. Microbiol. 2005;43(4):331-336.
DOI: https://doi.org/2257 [pii]
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AbstractAbstract
The objective of this study was to assess the strain-specificity of a DNA probe, Fu12, for Fusobacterium nucleatum subsp. nucleatum ATCC 25586^T (F. nucleatum ATCC 25586^T), and to develop sets of strain-specific polymerase chain reaction (PCR) primers. Strain-specificity was tested against 16 strains of F. nucleatum and 3 strains of distinct Fusobacterium species. Southern blot hybridization revealed that the Fu12 reacted exclusively with the HindIII-digested genomic DNA of F. nucleatum ATCC 25586^T. The results of PCR revealed that three pairs of PCR primers, based on the nucleotide sequence of Fu12, generated the strain-specific amplicons from F. nucleatum ATCC 25586^T. These results suggest that the DNA probe Fu12 and the three pairs of PCR primers could be useful in the identification of F. nucleatum ATCC 25586^T, especially with regard to the determination of the authenticity of the strain.
Rapid Detection of Bacteria from Blood Culture by an Electronic Nose
Peter Lykos , Pravin H. Patel , Christopher Morong , Asha Joseph
J. Microbiol. 2001;39(3):213-218.
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AbstractAbstract
The treatment of patients with bacteraemia and septicemia requires accurate and rapid identification of the pathogen so that the physician can be guided regarding the selection of the proper antimicrobial therapy. The usual procedure is to withdraw an aliquot of the positive blood culture sample for gram staining and subculturing on the media for the growth and subsequent identification, and susceptibility determinations. It was noticed that during the process some microbiologists would sniff the effluent gases that are products of metabolism and in some cases guess the identity of the bacterium. That prompted us to engage in systematic investigation of two gram positive and two gram negative bacteria using an electronic nose that had been proven successful in distinguishing the aroma of coffee beans from different sources. The investigation was successful in illustrating the efficacy of such a device in this clinical setting to distinguish Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis. A representative set of patterns obtained with this apparatus is displayed as well. No effort was made to determine an optimal set of sensors for some specific set of bacterial metabolism gaseous products.
Genotyping of Six Pathogenic Vibrio Species Based on RFLP of 16S rDNAs for Rapid Identification
Young-Jun Yoon , Kyung-Hwan Im , Young-Hwan Koh , Seong-Kon Kim , Jung-Wan Kim
J. Microbiol. 2003;41(4):312-319.
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AbstractAbstract
In an attempt to develop a method for rapid and accurate identification of six Vibrio species that are clinically important and most frequently detected in Korea, 16S rDNA restriction fragment length polymorphism (RFLP) of Vibrio type strains, as well as environmental isolates obtained from the Korean coastal area, was analyzed using ten restriction endonucleases. Digestion of the 16S rDNA fragments amplified by polymerase chain reaction (PCR) with the enzymes gave rise to 2~6 restriction patterns for each digestion for 47 Vibrio strains and isolates. An additional 2~3 restriction patterns were observed for five reference species, including Escherichia coli, Aeromonas hydrophila, A. salmonicida, Photobacterium phosphoreum, and Plesiomonas shigelloides. A genetic distance tree based on RFLP of the bacterial species correlated well with that based on 16S rDNA sequences. The very small 16S rDNA sequence difference (0.1%) between V. alginolyticus and V. parahaemolyticus was resolved clearly by RFLP with a genetic distance of more than 2%. RFLP variation within a species was also detected in the cases of V. parahaemolyticus, V. proteolyticus, and V. vulnificus. According to the RFLP analysis, six Vibrio and five reference species were assigned to 12 genotypes. Using three restriction endonucleases to analyze RFLP proved sufficient to identify the six pathogenic Vibrio species.

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