Prediction of Bacterial Proteins Carrying A Nuclear Localization Signal and Nuclear Targeting of HsdM from Klebsiella pneumoniae

Je Chul Lee; Dong Sun Kim; Dong Chan Moon; Jung-Hwa Lee; Mi Jin Kim; Su Man Lee; Yong Seok Lee; Se-Won Kang; Eun Jung Lee; Sang Sun Kang; Eunpyo Lee; Sung Hee Hyun

doi:10.1007/s12275-009-0217-4

Articles

Page Path: HOME > J. Microbiol > Volume 47(5); 2009 > Article

Research Support, Non-U.S. Gov't
Prediction of Bacterial Proteins Carrying A Nuclear Localization Signal and Nuclear Targeting of HsdM from Klebsiella pneumoniae: Je Chul Lee ¹, Dong Sun Kim ², Dong Chan Moon ¹, Jung-Hwa Lee ¹, Mi Jin Kim ², Su Man Lee ², Yong Seok Lee ³, Se-Won Kang ³, Eun Jung Lee ⁴, Sang Sun Kang ⁵, Eunpyo Lee ⁶, Sung Hee Hyun ⁴; Journal of Microbiology 2009;47(5):641-645.
DOI: https://doi.org/10.1007/s12275-009-0217-4
Published online: October 24, 2009

¹Department of Microbiology, Kyungpook National University School of Medicine, Daegu 702-701, Republic of Korea, ²Anatomy, Kyungpook National University School of Medicine, Daegu 702-701, Republic of Korea, ³Department of Parasitology, College of Medicine and Frontier Inje Research for Science and Technology, Inje University, Busan 614-735, Republic of Korea, ⁴Department of Biomedical Laboratory Science, Eulji University, School of Medicine, Daejeon 301-746, Republic of Korea, ⁵School of Science Education, Chungbuk National University, Chungbuk 361-763, Republic of Korea, ⁶Department of Medicine, Eulji University, Daejeon 301-746, Republic of Korea

Corresponding author: Sung Hee Hyun , Tel: 82-42-259-1751,

Received: 16 July 2009 • Accepted: 4 August 2009

prev next

277 Views
0 Download
14 Crossref
18 Scopus

Full Article

Download PDF

Abstract

Nuclear targeting of bacterial proteins is an emerging pathogenic mechanism whereby bacterial proteins can interact with nuclear molecules and alter the physiology of host cells. The fully sequenced bacterial genome can predict proteins that target the nuclei of host cells based on the presence of nuclear localization signal (NLS). In the present study, we predicted bacterial proteins with the NLS sequences from Klebsiella pneumoniae by bioinformatic analysis, and 13 proteins were identified as carrying putative NLS sequences. Among them, HsdM, a subunit of KpnAI that is a type I restriction-modification system found in K. pneumoniae, was selected for the experimental proof of nuclear targeting in host cells. HsdM carried the NLS sequences, 7KKAKAKK13, in the N-terminus. A transient expression of HsdM-EGFP in COS-1 cells exhibited exclusively a nuclear localization of the fusion proteins, whereas the fusion proteins of HsdM with substitutions in residues lysine to alanine in the NLS sequences, 7AAAKAAA13, were localized in the cytoplasm. HsdM was co-localized with importin α in the nuclei of host cells. Recombinant HsdM alone methylated the eukaryotic DNA in vitro assay. Although HsdM tested in this study has not been considered to be a virulence factor, the prediction of NLS motifs from the full sequenced genome of bacteria extends our knowledge of functional genomics to understand subcellular targeting of bacterial proteins.

Keywords: nuclear localization signal;DNA methylation;pathogenesis;K. pneumoniae

Figure & Data

References

Citations

Citations to this article as recorded by

Nuclear localization signal in Leishmania spp.: Implications for parasite physiology and host nucleus hijacking
Lara da Mata Aredes Riguetti, Carlos Roberto Alves, Franklin Souza da Silva
Protist.2026; 180: 126145. CrossRef
Risk factors for invasive Klebsiella pneumoniae liver abscess syndrome: a meta-analysis
Aimin Chen, Zhilin Luo, Yibin Peng
Frontiers in Cellular and Infection Microbiology.2026;[Epub] CrossRef
Nucleomodulins from gut bacteria: diverse mechanisms of translocation and interaction with host nuclear processes
Sania Korgaonkar, Chandrani Bose, Swadha Anand, Issac Cann
Applied and Environmental Microbiology.2025;[Epub] CrossRef
To target or not to target? The role of DNA and histone methylation in bacterial infections
Magdalena Barbachowska, Paola B. Arimondo
Epigenetics.2023;[Epub] CrossRef
FHA domain of AGGF1 is essential for its nucleocytoplasmic transport and angiogenesis
Cui-Fang Zhang, Han-Ming Wang, Andong Wu, Yang Li, Xiao-Li Tian
Science China Life Sciences.2021; 64(11): 1884. CrossRef
Origin of biomolecular games: deception and molecular evolution
Steven E. Massey, Bud Mishra
Journal of The Royal Society Interface.2018; 15(146): 20180429. CrossRef
Potential role of Escherichia coli DNA mismatch repair proteins in colon cancer
Shahanavaj Khan
Critical Reviews in Oncology/Hematology.2015; 96(3): 475. CrossRef
Prediction of Nuclear Targeting Proteins with Nuclear Localization Signals inStaphylococcus aureusand Nuclear Targeting of β-lactamase in Host Cells
Sung Hoon Ahn, Jung Hwa Lee, Je Chul Lee
Journal of Bacteriology and Virology.2015; 45(1): 36. CrossRef
Morphological changes in human gastric epithelial cells induced by nuclear targeting of Helicobacter pylori urease subunit A
Jung Hwa Lee, So Hyun Jun, Jung-Min Kim, Seung Chul Baik, Je Chul Lee
Journal of Microbiology.2015; 53(6): 406. CrossRef
Helicobacter pylori DNA methyltransferases and the epigenetic field effect in cancerization
Ramakrishnan Sitaraman
Frontiers in Microbiology.2014;[Epub] CrossRef
Functional eukaryotic nuclear localization signals are widespread in terminal proteins of bacteriophages
Modesto Redrejo-Rodríguez, Daniel Muñoz-Espín, Isabel Holguera, Mario Mencía, Margarita Salas
Proceedings of the National Academy of Sciences.2012; 109(45): 18482. CrossRef
Nuclear Translocation of Acinetobacter baumannii Transposase Induces DNA Methylation of CpG Regions in the Promoters of E-cadherin Gene
Dong Chan Moon, Chul Hee Choi, Su Man Lee, Jung Hwa Lee, Seung Il Kim, Dong Sun Kim, Je Chul Lee, Riccardo Manganelli
PLoS ONE.2012; 7(6): e38974. CrossRef
Screening of nuclear targeting proteins in Acinetobacter baumannii based on nuclear localization signals
Dong Chan Moon, Mamata Gurung, Jung Hwa Lee, Yong Seok Lee, Chi Won Choi, Seung Il Kim, Je Chul Lee
Research in Microbiology.2012; 163(4): 279. CrossRef
Prediction and screening of nuclear targeting proteins with nuclear localization signals in Helicobacter pylori
Jung Hwa Lee, So Hyun Jun, Seung Chul Baik, Deok Ryong Kim, Jae-Yong Park, Yong Seok Lee, Chul Hee Choi, Je Chul Lee
Journal of Microbiological Methods.2012; 91(3): 490. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Prediction of Bacterial Proteins Carrying A Nuclear Localization Signal and Nuclear Targeting of HsdM from Klebsiella pneumoniae

J. Microbiol. 2009;47(5):641-645. Published online October 24, 2009

DOI: https://doi.org/10.1007/s12275-009-0217-4

Related articles