DRA0336, Another OxyR Homolog, Involved in the Antioxidation Mechanisms in Deinococcus radiodurans

Longfei Yin; Liangyan Wang; Huiming Lu; Guangzhi Xu; Huan Chen; Hongdan Zhan; Bing Tian; Yuejin Hua

doi:10.1007/s12275-010-0043-8

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DRA0336, Another OxyR Homolog, Involved in the Antioxidation Mechanisms in Deinococcus radiodurans: Longfei Yin ¹, Liangyan Wang ¹, Huiming Lu ¹, Guangzhi Xu ¹, Huan Chen ^1,2, Hongdan Zhan ¹, Bing Tian ¹, Yuejin Hua ¹; Journal of Microbiology 2010;48(4):473-479.
DOI: https://doi.org/10.1007/s12275-010-0043-8
Published online: August 20, 2010

¹Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, P. R. China, ²Zhejiang Institute of Microbiology, Hangzhou 310027, P. R. China¹Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, P. R. China, ²Zhejiang Institute of Microbiology, Hangzhou 310027, P. R. China

Corresponding author: Bing Tian , Tel: +86-571-8697-1703,
Yuejin Hua , Tel: +86-571-8697-1703,

Received: 1 February 2010 • Accepted: 26 March 2010

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Abstract

A novel OxyR (DR0615) with one conserved cysteine that senses hydrogen peroxide in Deinococcus radiodurans had been identified in our previous work. Comparative genomics revealed that D. radiodurans possesses another OxyR homolog, OxyR2 (DRA0336). In this study, we constructed the deletion mutant of oxyR2 and the double mutant of both the OxyR homologs to investigate the role of OxyR in response to oxidative stress in D. radiodurans. Deletion of oxyR2 resulted in an obviously increased sensitivity to hydrogen peroxide, and the double mutant for oxyR and oxyR2 was significantly more sensitive than any of the two single mutants. The total catalase activity of the double mutant was lower than that of any of the single mutants, and reactive oxygen species (ROS) accumulated to a greater extent. DNA microarray analysis further suggested that oxyR2 was involved in antioxidation mechanisms. Site-direct mutagenesis and complementation analysis revealed that C228 in OxyR2 was essential. This is the first report of the presence of two OxyR in one organism. These results suggest that D. radiodurans OxyR and OxyR2 function together to protect the cell against oxidative stress.

Keywords: Deinococcus;OxyR;OxyR2;antioxidation mechanism;oxidative stress

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DRA0336, Another OxyR Homolog, Involved in the Antioxidation Mechanisms in Deinococcus radiodurans

J. Microbiol. 2010;48(4):473-479. Published online August 20, 2010

DOI: https://doi.org/10.1007/s12275-010-0043-8

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