Growth Inhibition of the Yeast Transformant by the Expression of a Chitinase from Coprinellus congregatus

Hyangsoon Lim; Hyoung T. Choi

doi:10.1007/s12275-010-0272-x

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Growth Inhibition of the Yeast Transformant by the Expression of a Chitinase from Coprinellus congregatus: Hyangsoon Lim , Hyoung T. Choi; Journal of Microbiology 2010;48(5):706-708.
DOI: https://doi.org/10.1007/s12275-010-0272-x
Published online: November 3, 2010

Department of Biochemistry, Kangwon National University, Chunchon 200-701, Republic of Korea

Corresponding author: Hyoung T. Choi , Tel: +82-33-250-8511,

Received: 22 July 2010 • Accepted: 5 October 2010

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Abstract

Coprinellus congregatus generates several chitinases during its entire life cycle: at the growing hyphal stage and at the mushroom autolysis stage. We have isolated a chitinase gene (chi1) from the mushroom tissue at the autolysing stage, and constructed a chitinase expression vector to get large amount of enzyme protein. Chitinase 1 (chi1) cDNA was heterologously expressed in Saccharomyces cerevisiae by gal1 promoter. The transformants showed no specific change in growth characteristics under normal growth conditions. However the expression of the gene by the gal1 promoter in the yeast transformants resulted in complete growth inhibition, while laccase expression by the gal1 promoter showed normal growth. The chitinase activities from the transformants were also more than 3 times higher than that of the recipient strain, and the chitinase expression by the real time-PCR also showed increased expression of the chi1 in the yeast transformant. Expression of a chitinase which was produced at the mushroom autolysing stage of C. congregatus resulted in yeast growth inhibition.

Keywords: chitinase;C. congregatus;heterologous expression;inky cap;yeast growth inhibition

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Growth Inhibition of the Yeast Transformant by the Expression of a Chitinase from Coprinellus congregatus

J. Microbiol. 2010;48(5):706-708. Published online November 3, 2010

DOI: https://doi.org/10.1007/s12275-010-0272-x

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