Role of Hydrogen Generation by Klebsiella pneumoniae in the Oral Cavity

Tomoko Kanazuru; Eisuke F. Sato; Kumiko Nagata; Hiroshi Matsui; Kunihiko Watanabe; Emiko Kasahara; Mika Jikumaru; June Inoue; Masayasu Inoue

doi:10.1007/s12275-010-0149-z

Articles

Page Path: HOME > J. Microbiol > Volume 48(6); 2010 > Article

Research Support, Non-U.S. Gov't
Role of Hydrogen Generation by Klebsiella pneumoniae in the Oral Cavity: Tomoko Kanazuru ^1,2, Eisuke F. Sato ¹, Kumiko Nagata ³, Hiroshi Matsui ², Kunihiko Watanabe ², Emiko Kasahara ¹, Mika Jikumaru ¹, June Inoue ⁴, Masayasu Inoue ¹; Journal of Microbiology 2010;48(6):778-783.
DOI: https://doi.org/10.1007/s12275-010-0149-z
Published online: January 9, 2011

¹Department of Biochemistry & Molecular Pathology, Osaka City University Medical School, Osaka 545-8585, Japan, ²Department of Applied Biochemistry, Kyoto Prefectural University, Kyoto 606-8522, Japan, ³Department of Food and Nutrition, Senri Kinran University, Shita 565-0873, Japan, ⁴Department of Gastroenterology, Kobe University Medical School, Kobe 650-0017, Japan¹Department of Biochemistry & Molecular Pathology, Osaka City University Medical School, Osaka 545-8585, Japan, ²Department of Applied Biochemistry, Kyoto Prefectural University, Kyoto 606-8522, Japan, ³Department of Food and Nutrition, Senri Kinran University, Shita 565-0873, Japan, ⁴Department of Gastroenterology, Kobe University Medical School, Kobe 650-0017, Japan

Corresponding author: Eisuke F. Sato , Tel: +81-6-6645-3722,

Received: 16 April 2010 • Accepted: 11 August 2010

prev next

53 Views
0 Download
12 Crossref
15 Scopus

Full Article

Abstract

Some gastrointestinal bacteria synthesize hydrogen (H2) by fermentation. Despite the presence of bactericidal factors in human saliva, a large number of bacteria also live in the oral cavity. It has never been shown that oral bacteria also produce H2 or what role H2 might play in the oral cavity. It was found that a significant amount of H2 is synthesized in the oral cavity of healthy human subjects, and that its generation is enhanced by the presence of glucose but inhibited by either teeth brushing or sterilization with povidone iodine. These observations suggest the presence of H2-generating bacteria in the oral cavity. The screening of commensal bacteria in the oral cavity revealed that a variety of anaerobic bacteria generate H2. Among them, Klebsiella pneumoniae (K. pneumoniae) generated significantly large amounts of H2 in the presence of glucose. Biochemical analysis revealed that various proteins in K. pneumoniae are carbonylated under standard culture conditions, and that oxidative stress induced by the presence of Fe++ and H2O2 increases the number of carbonylated proteins, particularly when their hydrogenase activity is inhibited by KCN. Inhibition of H2 generation markedly suppresses the growth of K. pneumoniae. These observations suggest that H2 generation and/or the reduction of oxidative stress is important for the survival and growth of K. pneumoniae in the oral cavity.

Keywords: hydrogen;hydrogenase;oral cavity;K. pneumoniae

Figure & Data

References

Citations

Citations to this article as recorded by

Mechanical Energy Drives the Growth and Carbon Fixation of Electroactive Microorganisms
Guoping Ren, Jie Ye, Lu Liu, Andong Hu, Kenneth H. Nealson, Christopher Rensing, Shungui Zhou
Engineering.2025; 47: 194. CrossRef
The oral-gut microbiome axis in inflammatory bowel disease: from inside to insight
Aili Wang, Zihan Zhai, Yiyun Ding, Jingge Wei, Zhiqiang Wei, Hailong Cao
Frontiers in Immunology.2024;[Epub] CrossRef
Targeting bacterial nickel transport with aspergillomarasmine A suppresses virulence-associated Ni-dependent enzymes
David Sychantha, Xuefei Chen, Kalinka Koteva, Gerd Prehna, Gerard D. Wright
Nature Communications.2024;[Epub] CrossRef
The On/Off History of Hydrogen in Medicine: Will the Interest Persist This Time Around?
Tyler W. LeBaron, Kinji Ohno, John T. Hancock
Oxygen.2023; 3(1): 143. CrossRef
Relationships Between Oral Microecosystem and Respiratory Diseases
Jiajia Dong, Wei Li, Qi Wang, Jiahao Chen, Yue Zu, Xuedong Zhou, Qiang Guo
Frontiers in Molecular Biosciences.2022;[Epub] CrossRef
Methanobrevibacter smithii Archaemia in Febrile Patients With Bacteremia, Including Those With Endocarditis
Michel Drancourt, Kenza Djemai, Frédérique Gouriet, Ghiles Grine, Ahmed Loukil, Marielle Bedotto, Anthony Levasseur, Hubert Lepidi, Jacques Bou-Khalil, Saber Khelaifia, Didier Raoult
Clinical Infectious Diseases.2021; 73(9): e2571. CrossRef
Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists
Stéphane L. Benoit, Robert J. Maier, R. Gary Sawers, Chris Greening
Microbiology and Molecular Biology Reviews.2020;[Epub] CrossRef
Role of Nickel in Microbial Pathogenesis
Robert Maier, Stéphane Benoit
Inorganics.2019; 7(7): 80. CrossRef
Impact of Klebsiella pneumoniae in lower gastrointestinal tract diseases
Christina Parvinder Kaur, Jamuna Vadivelu, Samudi Chandramathi
Journal of Digestive Diseases.2018; 19(5): 262. CrossRef
Site-directed mutagenesis of Campylobacter concisus respiratory genes provides insight into the pathogen’s growth requirements
Stéphane L. Benoit, Robert J. Maier
Scientific Reports.2018;[Epub] CrossRef
Markers of Perioperative Bowel Complications in Colorectal Surgery Patients
Radomír Hyšpler, Alena Tichá, Milan Kaška, Lenka Žaloudková, Lenka Plíšková, Eduard Havel, Zdeněk Zadák
Disease Markers.2015; 2015: 1. CrossRef
Hydrogenase: the next antibiotic target?
Wei Nie, Hao Tang, Zheng Fang, Jiquan Chen, Haili Chen, Qingyu Xiu
Clinical Science.2012; 122(12): 575. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Role of Hydrogen Generation by Klebsiella pneumoniae in the Oral Cavity

J. Microbiol. 2010;48(6):778-783. Published online January 9, 2011

DOI: https://doi.org/10.1007/s12275-010-0149-z

Related articles