Improved Antibiotic Resistance Gene Cassette for Marker Exchange Mutagenesis in Ralstonia solanacearum and Burkholderia Species

Hae Young Um; Eunsook Chung; Jai-Heon Lee; Seon-Woo Lee

doi:10.1007/s12275-011-0439-0

Skip Navigation: Skip to contents

SEARCH: Search

Advanced Search

mobile menu button

Articles

Page Path: HOME > J. Microbiol > Volume 49(2); 2011 > Article

Research Support, Non-U.S. Gov't
Improved Antibiotic Resistance Gene Cassette for Marker Exchange Mutagenesis in Ralstonia solanacearum and Burkholderia Species: Hae Young Um ¹, Eunsook Chung ¹, Jai-Heon Lee ¹, Seon-Woo Lee ^1,2; Journal of Microbiology 2011;49(2):305-308.
DOI: https://doi.org/10.1007/s12275-011-0439-0
Published online: May 3, 2011

¹Department of Medical Bioscience, Dong-A University, Busan 604-714, Republic of Korea, ²Department of Applied Biology, Dong-A University, Busan 604-714, Republic of Korea

Corresponding author: Seon-Woo Lee , Tel: +82-51-200-7551,

Received: 10 October 2010 • Accepted: 29 November 2010

prev next

311 Views
0 Download
1 Crossref
2 Scopus

Full Article

Download PDF

Abstract

Marker exchange mutagenesis is a fundamental approach to understanding gene function at a molecular level in bacteria. New plasmids carrying a kanamycin resistance gene or a trimethoprim resistance gene were constructed to provide antibiotic resistance cassettes for marker exchange mutagenesis in Ralstonia solanacearum and many antibiotic-resistant Burkholderia spp. Insertion sequences present in the flanking sequences of the antibiotic resistance cassette were removed to prevent aberrant gene replacement and polar mutation during mutagenesis in wild-type bacteria. Plasmids provided in this study would be convenient for use in gene cassettes for gene replacement in other Gram-negative bacteria.

Keywords: antibiotic resistance;kanamycin;marker exchange;Ralstonia solanacearum;trimethoprim

Figure & Data

References

Citations

Citations to this article as recorded by

ToxB encodes a canonical GTP cyclohydrolase II in toxoflavin biosynthesis and ribA expression restored toxoflavin production in a ΔtoxB mutant
Minae Joo, Hye-Gyeong Yoo, Hyun-Ju Kim, Hyung-Jin Kwon
Journal of the Korean Society for Applied Biological Chemistry.2015; 58(6): 877. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Improved Antibiotic Resistance Gene Cassette for Marker Exchange Mutagenesis in Ralstonia solanacearum and Burkholderia Species

J. Microbiol. 2011;49(2):305-308. Published online May 3, 2011

DOI: https://doi.org/10.1007/s12275-011-0439-0

Related articles

TOP