NOTE] A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi

Yuanyuan Chen; Bernard A. Prior; Guiyang Shi; Zhengxiang Wang

doi:10.1007/s12275-011-0525-3

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NOTE] A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi: Yuanyuan Chen ¹, Bernard A. Prior ², Guiyang Shi ¹, Zhengxiang Wang ¹; Journal of Microbiology 2011;49(4):675-679.
DOI: https://doi.org/10.1007/s12275-011-0525-3
Published online: September 2, 2011

¹The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, P. R. China, ²Department of Microbiology, School of Biological Sciences, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa

Corresponding author: Zhengxiang Wang , Tel: +86-510-85918121,

Received: 17 December 2010 • Accepted: 18 March 2011

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Abstract

In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates.

Keywords: filamentous fungi;genomic DNA extraction;alkaline lysis;internal transcribed spacer regions;identification

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NOTE] A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi

J. Microbiol. 2011;49(4):675-679. Published online September 2, 2011

DOI: https://doi.org/10.1007/s12275-011-0525-3

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