Detecting Nonculturable Bacteria in the Active Mycorrhizal Zone of the Pine Mushroom Tricholoma matsutake

Ryota Kataoka; Zaki Anwar Siddiqui; Junichi Kikuchi; Masaki Ando; Rina Sriwati; Ai Nozaki; Kazuyoshi Futai

doi:10.1007/s12275-012-1371-7

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Detecting Nonculturable Bacteria in the Active Mycorrhizal Zone of the Pine Mushroom Tricholoma matsutake: Ryota Kataoka ¹, Zaki Anwar Siddiqui ^1,2, Junichi Kikuchi ³, Masaki Ando ⁴, Rina Sriwati ^1,5, Ai Nozaki ⁴, Kazuyoshi Futai ¹; Journal of Microbiology 2012;50(2):199-206.
DOI: https://doi.org/10.1007/s12275-012-1371-7
Published online: April 27, 2012

¹Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan, ²Department of Botany, Aligarh Muslim University, Aligarh-202002, India, ³Science Education, Nara University of Education, Takabatake-cho, Nara 630-8528, Japan, ⁴Kyoto Forest Research Institute, Kyotanba-cho honjyo, Funai-gun, Kyoto 629-1121, Japan, ⁵Field of Plant Protection, Syiah Kuala University, Darussalam Banda Aceh 23111, Indonesia¹Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan, ²Department of Botany, Aligarh Muslim University, Aligarh-202002, India, ³Science Education, Nara University of Education, Takabatake-cho, Nara 630-8528, Japan, ⁴Kyoto Forest Research Institute, Kyotanba-cho honjyo, Funai-gun, Kyoto 629-1121, Japan, ⁵Field of Plant Protection, Syiah Kuala University, Darussalam Banda Aceh 23111, Indonesia

Corresponding author: Kazuyoshi Futai , Tel: +81-75-753-2266,

Received: 29 July 2011 • Accepted: 8 December 2011

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Abstract

The fungus Tricholoma matsutake forms an ectomycorrhizal relationship with pine trees. Its sporocarps often develop in a circle, which is commonly known as a fairy ring. The fungus produces a solid, compact, white aggregate of mycelia and mycorrhizae beneath the fairy ring, which in Japanese is called a ‘shiro’. In the present study, we used soil dilution plating and molecular techniques to analyze the bacterial communities within, beneath, and outside the T. matsutake fairy ring. Soil dilution plating confirmed previous reports that bacteria and actinomycetes are seldom present in the soil of the active mycorrhizal zone of the T. matsutake shiro. In addition, the results showed that the absence of bacteria was strongly correlated with the presence of T. matsutake mycorrhizae. The results demonstrate that bacteria, especially aerobic and heterotrophic forms, and actinomycetes, are strongly inhibited by T. matsutake. Indeed, neither bacteria nor actinomycetes were detected in 11.3% of 213 soil samples from the entire shiro area by culture-dependent
methods
. However, molecular techniques demonstrated that some bacteria, such as individual genera of Sphingomonas and Acidobacterium, were present in the active mycorrhizal zone, even though they were not detected in soil assays using the dilution plating technique.

Keywords: bacterial communities;bacterial populations;PCR-DGGE;Shiro;Tricholoma matsutake

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Detecting Nonculturable Bacteria in the Active Mycorrhizal Zone of the Pine Mushroom Tricholoma matsutake

J. Microbiol. 2012;50(2):199-206. Published online April 27, 2012

DOI: https://doi.org/10.1007/s12275-012-1371-7

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