Identification and Enumeration of Microcystis Using a Sandwich Hybridization Assay

Jing Ping Zhu; Xian Li; Shi Du

doi:10.1007/s12275-012-1418-9

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Identification and Enumeration of Microcystis Using a Sandwich Hybridization Assay: Jing Ping Zhu , Xian Li , Shi Du; Journal of Microbiology 2012;50(2):186-190.
DOI: https://doi.org/10.1007/s12275-012-1418-9
Published online: April 27, 2012

School of Environmental Science and Engineering, Huazhong University of Science & Technology, Wuhan 430074, P. R. ChinaSchool of Environmental Science and Engineering, Huazhong University of Science & Technology, Wuhan 430074, P. R. China

Corresponding author: Jing Ping Zhu , Tel: +86-27-8779-2101,

Received: 24 August 2011 • Accepted: 18 November 2011

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Abstract

Based on sequence analyses of phycocyanin intergenic spacers (PC-IGS) from Microcystis, Anabaena, Aphanizomenon, and Planktothrix (Oscillatoria) strains, a genus-specific probe pair TF/TR was designed, and a sandwich hybridization assay was established to quantitatively detect Microcystis. Through BLAST and cyanobacterial culture tests, TF/TR was demonstrated to be specific for Microcystis. A calibration curve for the sandwich hybridization assay was established, and the lowest detected concentration was 100 cell/ml. Laboratory and field samples were analyzed with both sandwich hybridization assay and microscopy. The biotic and abiotic components of the samples were of little disturbance to the sandwich hybridization assay. The results showed no distinct difference between the two methods. In this study, a sandwich hybridization assay was established to detect Microcystis, providing an alternative to traditional microscopic, morphology- based methods.

Keywords: Microcystis;molecular probes;sandwich hybridization assay;PC-IGS

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Identification and Enumeration of Microcystis Using a Sandwich Hybridization Assay

J. Microbiol. 2012;50(2):186-190. Published online April 27, 2012

DOI: https://doi.org/10.1007/s12275-012-1418-9

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