NOTE] Effects of Nutritional Enrichment on the Production of Acetone-Butanol-Ethanol (ABE) by Clostridium acetobutylicum

Sung Jun Choi; Joungmin Lee; Yu-Sin Jang; Jin Hwan Park; Sang Yup Lee; In Ho Kim

doi:10.1007/s12275-012-2373-1

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Research Support, Non-U.S. Gov't
NOTE] Effects of Nutritional Enrichment on the Production of Acetone-Butanol-Ethanol (ABE) by Clostridium acetobutylicum: Sung Jun Choi ^1,2, Joungmin Lee ¹, Yu-Sin Jang ¹, Jin Hwan Park ¹, Sang Yup Lee ^1,3, In Ho Kim ³; Journal of Microbiology 2012;50(6):1063-1066
DOI: https://doi.org/10.1007/s12275-012-2373-1
Published online: December 30, 2012

¹Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305-701, Republic of Korea, ²Department of Chemical Engineering, Chungnam National University, Deajeon 305-764, Republic of Korea, ³Department of Bio and Brain Engineering and Bioinformatics Research Center, Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea¹Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305-701, Republic of Korea, ²Department of Chemical Engineering, Chungnam National University, Deajeon 305-764, Republic of Korea, ³Department of Bio and Brain Engineering and Bioinformatics Research Center, Department of Biological Sciences, KAIST, Daejeon 305-701, Republic of Korea

Corresponding author: In Ho Kim , Tel: +82-42-350-8818,

Received: 23 July 2012 • Accepted: 28 August 2012

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Abstract

Clostridium acetobutylicum is an industrially important organism that produces acetone-butanol-ethanol (ABE). The main objective of this study was to characterize the effects of increased cell density on the production of ABE during the phase transition from acidogenesis to solventogenesis in C. acetobutylicum. The increased ABE productivity of C. acetobutylicum was obtained by increasing the cell density using a newly designed medium (designated C. acetobutylicum medium 1; CAM1). The maximum OD600 value of C. acetobutylicum ATCC 824 strain obtained with CAM1 was 19.7, which is 1.8 times higher than that obtained with clostridial growth medium (CGM). The overall ABE productivity obtained in the CAM1-fermetation of the ATCC 824 strain was 0.83 g/L/h, which is 1.5 times higher than that (0.55 g/L/h) obtained with CGM. However, the increased productivity obtained with CAM1 did not result in an increase in the final ABE titer, because phase transition occurred at a high titer of acids.

Keywords: ABE; cell density; Clostridium acetobutylicum; phase transition; productivity

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NOTE] Effects of Nutritional Enrichment on the Production of Acetone-Butanol-Ethanol (ABE) by Clostridium acetobutylicum

J. Microbiol. 2012;50(6):1063-1066. Published online December 30, 2012

DOI: https://doi.org/10.1007/s12275-012-2373-1

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