Characterization of Cellulases of Fungal Endophytes Isolated from Espeletia spp.

Luisa Cabezas; Carolina Calderon; Luis Miguel Medina; Isabela Bahamon; Martha Cardenas; Adriana Jimena Bernal; Andrés Gonzalez; Silvia Restrepo

doi:10.1007/s12275-012-2130-5

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Characterization of Cellulases of Fungal Endophytes Isolated from Espeletia spp.: Luisa Cabezas ¹, Carolina Calderon ¹, Luis Miguel Medina ², Isabela Bahamon ², Martha Cardenas ¹, Adriana Jimena Bernal ¹, Andrés Gonzalez ², Silvia Restrepo ¹; Journal of Microbiology 2012;50(6):1009-1013.
DOI: https://doi.org/10.1007/s12275-012-2130-5
Published online: December 30, 2012

¹Laboratorio de Micología y Fitopatología (LAMFU), Departamento de Ciencias Biológicas Universidad de Los Andes, Bogotá, Colombia, ²Grupo de Diseño de productos y Procesos (GDPP), Departamento de Ingenieria. Universidad de Los Andes, Bogotá, Colombia

Corresponding author: Silvia Restrepo , Tel: +571-339-4949,

Received: 8 March 2012 • Accepted: 4 July 2012

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Abstract

Endophytes are microorganisms that asymptomatically invade plant tissues. They can stimulate plant growth and/or provide defense against pathogen attacks through the production of secondary metabolites. Most endophyte species are still unknown, and because they may have several applications, the study of their metabolic capabilities is essential. We characterized 100 endophytes isolated from Espeletia spp., a genus unique to the paramo ecosystem, an extreme environment in the Andean mountain range. We evaluated the cellulolytic potential of these endophytes on the saccharification of the oil palm empty fruit bunch (OPEFB). The total cellulolytic activity was measured for each endophyte on filter paper (FPA). In addition, the specific carboxymethyl cellulase (CMCase), exoglucanase, and β-glucosidase activities were determined. We found four fungi positive for cellulases. Of these fungi, Penicillium glabrum had the highest cellulolytic activity after partial purification, with maximal CMCase, exoglucanase and β-glucosidase enzyme activities of 44.5, 48.3, and 0.45 U/ml, respectively. Our data showed that the bioprospection of fungi and the characterization of their enzymes may facilitate the process of biofuel production.

Keywords: endophytes;antimicrobial activity;cellulases;OPEFB;Penicillium

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Characterization of Cellulases of Fungal Endophytes Isolated from Espeletia spp.

J. Microbiol. 2012;50(6):1009-1013. Published online December 30, 2012

DOI: https://doi.org/10.1007/s12275-012-2130-5

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