Catabolite Control Protein A of Streptococcus suis Type 2 Contributes to Sugar Metabolism and Virulence

Yulong Tang; Wei Wu; Xiaoyan Zhang; Zhongyan Lu; Jianshun Chen; Weihuan Fang

doi:10.1007/s12275-012-2035-3

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Catabolite Control Protein A of Streptococcus suis Type 2 Contributes to Sugar Metabolism and Virulence: Yulong Tang , Wei Wu , Xiaoyan Zhang , Zhongyan Lu , Jianshun Chen , Weihuan Fang; Journal of Microbiology 2012;50(6):994-1002.
DOI: https://doi.org/10.1007/s12275-012-2035-3
Published online: December 30, 2012

Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, P. R. China

Corresponding author: Weihuan Fang , Tel: +86-571-88982242,

Received: 26 January 2012 • Accepted: 21 August 2012

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Abstract

Catabolite control protein A (CcpA) is the major transcriptional regulator in carbon catabolite repression in several Gram-positive bacteria. We attempted to characterize the role of a CcpA homologue of Streptococcus suis type 2 in sugar metabolism and virulence. Addition of glucose or sucrose to the defined medium significantly reduced the activity of raffinose-inducible α-galactosidase, cellobiose-inducible β-glucosidase, and maltose-inducible α-glucosidase of the wildtype strain by about 9, 4, and 2-3 fold, respectively. Deletion of ccpA substantially derepressed the effects of repressing sugars on α-galactosidase or β-glucosidase activity. The ccpA deletion mutant showed reduced expression of virulence genes sly and eno (P<0.05), decreased adhesion to and invasion into endothelial cells (P<0.05), and attenuated virulence to mice with significant reduction of death rate and bacterial burden in organs, as compared to the wild-type strain. Both the in vitro and in vivo defect phenotypes were reversible by ccpA complementation. Thus, this study shows that CcpA of S. suis type 2 plays an important role in carbon catabolite repression and virulence.

Keywords: Streptoccoccus suis type 2;catabolite control protein A;carbon catabolite repression;virulence

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Catabolite Control Protein A of Streptococcus suis Type 2 Contributes to Sugar Metabolism and Virulence

J. Microbiol. 2012;50(6):994-1002. Published online December 30, 2012

DOI: https://doi.org/10.1007/s12275-012-2035-3

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