Construction of a Streptomyces lydicus A01 Transformant with a chit42 Gene from Trichoderma harzianum P1 and Evaluation of Its Biocontrol Activity against Botrytis cinerea

Qiong Wu; Linquan Bai; Weicheng Liu; Yingying Li; Caige Lu; Yaqian Li; Kehe Fu; Chuanjin Yu; Jie Chen

doi:10.1007/s12275-013-2321-8

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Research Support, Non-U.S. Gov't
Construction of a Streptomyces lydicus A01 Transformant with a chit42 Gene from Trichoderma harzianum P1 and Evaluation of Its Biocontrol Activity against Botrytis cinerea: Qiong Wu ¹, Linquan Bai ², Weicheng Liu ³, Yingying Li ¹, Caige Lu ³, Yaqian Li ¹, Kehe Fu ¹, Chuanjin Yu ¹, Jie Chen ¹; Journal of Microbiology 2013;51(2):166-173.
DOI: https://doi.org/10.1007/s12275-013-2321-8
Published online: April 27, 2013

¹School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China, ²School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, P. R. China, ³Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P. R. China¹School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China, ²School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, P. R. China, ³Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P. R. China

Corresponding author: Jie Chen , Tel: +86-21-34206141,

Received: 21 June 2012 • Accepted: 13 November 2012

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Abstract

Streptomyces lydicus A01 and Trichoderma harzianum P1 are potential biocontrol agents of fungal diseases in plants. S. lydicus A01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of Botrytis cinerea. T. harzianum P1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of B. cinerea. To obtain the synergistic biocontrol effects of chitinase and natamycin on Botrytis cinerea, this study transformed the chit42 gene from T. harzianum P1 to S. lydicus A01. The conjugal transformant (CT) of S. lydicus A01 with the chit42 gene was detected using polymerase chain reaction (PCR). Associated chitinase activity and natamycin production were examined using the 3, 5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry, respectively. The S. lydicus A01-chit42 CT showed substantially higher chitinase activity and natamycin production than its wild type strain (WT). Consequently, the biocontrol effects of S. lydicus A01-chit42 CT on B. cinerea, including inhibition to spore germination and mycelial growth, were highly improved compared with those of the WT. Our research indicates that the biocontrol effect of Streptomyces can be highly improved by transforming the exogenous resistance gene, i.e. chit42 from Trichoderma, which not only enhances the production of antibiotics, but also provides a supplementary function by degrading the cell walls of the pathogens.

Keywords: Streptomyces lydicus A01;chit42 from Trichoderma harzianum P1;Botrytis cinerea;chitinase activity;natamycin production

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Construction of a Streptomyces lydicus A01 Transformant with a chit42 Gene from Trichoderma harzianum P1 and Evaluation of Its Biocontrol Activity against Botrytis cinerea

J. Microbiol. 2013;51(2):166-173. Published online April 27, 2013

DOI: https://doi.org/10.1007/s12275-013-2321-8

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