The N3 Subdomain in A Domain of Fibronectin-Binding Protein B Isotype I Is an Independent Risk Determinant Predictive for Biofilm Formation of Staphylococcus aureus Clinical Isolates

An Sung Kwon; Dong Hoon Lim; Hyo Jung Shin; Geon Park; Jong H. Reu; Hyo Jin Park; Jungmin Kim; Yong Lim

doi:10.1007/s12275-013-3319-y

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The N3 Subdomain in A Domain of Fibronectin-Binding Protein B Isotype I Is an Independent Risk Determinant Predictive for Biofilm Formation of Staphylococcus aureus Clinical Isolates: An Sung Kwon ¹, Dong Hoon Lim ², Hyo Jung Shin ¹, Geon Park ³, Jong H. Reu ¹, Hyo Jin Park ¹, Jungmin Kim ⁴, Yong Lim ¹; Journal of Microbiology 2013;51(4):499-505.
DOI: https://doi.org/10.1007/s12275-013-3319-y
Published online: August 30, 2013

¹Department of Microbiology, Medical School, Chosun University, Gwangju 501-759, Republic of Korea, ²Department of Urology, Medical School, Chosun University, Gwangju 501-759, Republic of Korea, ³Department of Laboratory Medicine, Medical School, Chosun University, Gwangju 501-759, Republic of Korea , ⁴Department of Microbiology, Kyungpook National University, School of Medicine, Daegu 700-422, Republic of Korea

Corresponding author: Jungmin Kim , Tel: +82-62-230-6393,
Yong Lim , Tel: +82-62-230-6393,

Received: 10 June 2013 • Accepted: 8 August 2013

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Abstract

Fibronectin-binding proteins (FnBP), FnBPA and FnBPB, are purported to be involved in biofilm formation of Staphylococcus aureus. This study was performed to find which of three consecutive N subdomains of the A domain in the FnBP is the key domain in FnBP. A total of 465 clinical isolates of S. aureus were examined for the biofilm forming capacity and the presence of N subdomains of FnBP. In the biofilm-positive strains, N2 and N3 subdomains of FnBPA, and N1 and N3 subdomains of FnBPB were significantly more prevalent. Multivariate logistic regression analysis of 246 biofilm-positive and 123 biofilm-negative strains identified only the FnBPB-N3 subdomain as an independent risk determinant predictive for biofilm-positive strains of S. aureus (Odds ratio [OR], 13.174; P<0.001). We also attempted to delete each of the fnbA-N2 and -N3 and fnbB-N1 and -N3 from S. aureus strain 8325-4 and examined the biofilm forming capacity in the derivative mutants. In agreement with the results of the multivariate regression analysis, deletion of either the fnbA-N2 or -N3, or fnbB-N1 did not significantly diminish the capacity of strain 8325-4 to develop a biofilm, while deletion of the fnbB-N3 did. Therefore, it is suggested that the FnBPB-N3 subdomain of isotype I may be a key domain in FnBP which is responsible for the causing biofilm formation in S. aureus clinical isolates.

Keywords: fibronectin-binding proteins;N3 subdomains of FnBPB;biofilm;S. aureus

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The N3 Subdomain in A Domain of Fibronectin-Binding Protein B Isotype I Is an Independent Risk Determinant Predictive for Biofilm Formation of Staphylococcus aureus Clinical Isolates

J. Microbiol. 2013;51(4):499-505. Published online August 30, 2013

DOI: https://doi.org/10.1007/s12275-013-3319-y

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