Trans-Membrane Transport of n-Octadecane by Pseudomonas sp. DG17

Fei Hua; Hong Qi Wang; Yi Li; Yi Cun Zhao

doi:10.1007/s12275-013-3259-6

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Trans-Membrane Transport of n-Octadecane by Pseudomonas sp. DG17: Fei Hua , Hong Qi Wang , Yi Li , Yi Cun Zhao; Journal of Microbiology 2013;51(6):791-799.
DOI: https://doi.org/10.1007/s12275-013-3259-6
Published online: December 19, 2013

College of Water Sciences, Beijing Normal University, Beijing 100875, P. R. ChinaCollege of Water Sciences, Beijing Normal University, Beijing 100875, P. R. China

Corresponding author: Hong Qi Wang , Tel: +86-010-58807810,

Received: 15 October 2012 • Accepted: 29 July 2013

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Abstract

The trans-membrane transport of hydrocarbons is an important and complex aspect of the process of biodegradation of hydrocarbons by microorganisms. The mechanism of transport of 14C n-octadecane by Pseudomonas sp. DG17, an alkane-degrading bacterium, was studied by the addition of ATP inhibitors and different substrate concentrations. When the concentration of n-octadecane was higher than 4.54 μmol/L, the transport of 14C n-octadecane was driven by a facilitated passive mechanism following the intra/extra substrate concentration gradient. However, when the cells were grown with a low concentration of the substrate, the cellular accumulation of n-octadecane, an energy-dependent process, was dramatically decreased by the presence of ATP inhibitors, and n-octadecane accumulation continually increased against its concentration gradient. Furthermore, the presence of non-labeled alkanes blocked 14C n-octadecane transport only in the induced cells, and the trans-membrane transport of n-octadecane was specific with an apparent dissociation constant Kt of 11.27 μmol/L and Vmax of 0.96 μmol/min/mg protein. The results indicated that the transmembrane transport of n-octadecane by Pseudomonas sp. DG17 was related to the substrate concentration and ATP.

Keywords: Pseudomonas;n-octadecane transport;facilitate;energy-dependent;specific

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Trans-Membrane Transport of n-Octadecane by Pseudomonas sp. DG17

J. Microbiol. 2013;51(6):791-799. Published online December 19, 2013

DOI: https://doi.org/10.1007/s12275-013-3259-6

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