Reducing the Bioactivity of Tannerella forsythia Lipopolysaccharide by Porphyromonas gingivalis

Young-Jae Kim; Sung-Hoon Lee

doi:10.1007/s12275-014-4324-5

Articles

Page Path: HOME > J. Microbiol > Volume 52(8); 2014 > Article

Journal Article
Reducing the Bioactivity of Tannerella forsythia Lipopolysaccharide by Porphyromonas gingivalis: Young-Jae Kim ¹, Sung-Hoon Lee ²; Journal of Microbiology 2014;52(8):702-708.
DOI: https://doi.org/10.1007/s12275-014-4324-5
Published online: August 1, 2014

¹Department of Pediatric Dentistry, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea, ²Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University, Cheonan 330-714, Republic of Korea¹Department of Pediatric Dentistry, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea, ²Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University, Cheonan 330-714, Republic of Korea

Corresponding author: Sung-Hoon Lee , Tel: +82-41-550-1867,

Received: 5 June 2014 • Revised: 23 June 2014 • Accepted: 27 June 2014

prev next

48 Views
0 Download
13 Crossref
11 Scopus

Full Article

Abstract

Tannerella forsythia is considered a pathogen of periodontitis and forms a biofilm with multi-species bacteria in oral cavity. Lipopolysaccharide is a powerful immunostimulator and induces inflammation and shock. The purpose of this study was to investigate the characteristics of T. forsythia LPS in its co-cultivation with Fusobacterium nucleatum or Porphyromonas gingivalis. T. forsythia was co-cultured in the presence and absence of F. nucleatum and P. gingivalis and then T. forsythia LPS was extracted. The extracts were analyzed by SDS-PAGE and NF-κB reporter CHO cell lines. THP-1 cells were treated with the LPS and evaluated induction of cytokine expression by real-time RT-PCR and ELISA. For analysis of the bioactivity of T. forsythia LPS, the binding assay on LPS-binding protein (LBP) and CD14 was processed. The extracts did not contaminate other molecules except LPS and showed TLR4 agonists. Co-cultured T. forsythia LPS with P. gingivalis exhibited a lower level of induction of TNF-α, IL-1β, and IL-6 expression than singleor co-cultured T. forsythia LPS with F. nucleatum in the conditions of human serum. However, the three T. forsythia LPS did not show difference of cytokine induction in the serum free conditions. Co-cultured T. forsythia LPS with P. gingivalis exhibited a lower affinity to LBP and CD14 as binding site of O-antigen and attached at a lower level to THP-1 cells compared to single- or co-cultured T. forsythia LPS with F. nucleatum. The virulence of T. forsythia LPS was decreased by co-culturing with P. gingivalis and their affinity to LBP and CD14 was reduced, which may due to modification of O-antigen chain by P. gingivalis.

Keywords: lipopolysaccharide;Tannerella forsythia;Porphyromonas gingivalis

Figure & Data

References

Citations

Citations to this article as recorded by

Anti-Inflammatory Efficacy of Human-Derived Streptococcus salivarius on Periodontopathogen-Induced Inflammation
Dong-Heon Baek, Sung-Hoon Lee
Journal of Microbiology and Biotechnology.2023; 33(8): 998. CrossRef
Virulence of Filifactor alocis lipoteichoic acid on human gingival fibroblast
Hyun-Jun Yoo, Sung-Hoon Lee
Archives of Oral Biology.2022; 135: 105370. CrossRef
Effects of Shiitake mushroom extract on antimicrobial activity against periodontopathogens and inflammatory condition of human gingival fibroblast
Yeol-Mae Jeon
Journal of Dental Rehabilitation and Applied Science.2022; 38(2): 90. CrossRef
Characteristics of Treponema denticola lipooligosaccharide in presence of hemin and quorum-sensing molecule
Dong-Heon Baek, Sung-Hoon Lee
Archives of Oral Biology.2021; 124: 105062. CrossRef
The Associations of Periodontopathic Bacteria and Oral Candida with Periodontal Inflamed Surface Area in Older Adults Receiving Supportive Periodontal Therapy
Hideo Shigeishi, Mariko Nakamura, Iori Oka, Cheng-Yih Su, Kanako Yano, Momoko Ishikawa, Yoshino Kaneyasu, Masaru Sugiyama, Kouji Ohta
Diagnostics.2021; 11(8): 1397. CrossRef
Efficacy of β-caryophyllene for periodontal disease related factors
Hyun-Jun Yoo, Su-Kyung Jwa
Archives of Oral Biology.2019; 100: 113. CrossRef
Inhibitory effect of Lactococcus lactis on the bioactivity of periodontopathogens
Hyun-Seung Shin, Dong-Heon Baek, Sung-Hoon Lee
The Journal of General and Applied Microbiology.2018; 64(2): 55. CrossRef
Consistent and reproducible long-term in vitro growth of health and disease-associated oral subgingival biofilms
Irina M. Velsko, Luciana M. Shaddox
BMC Microbiology.2018;[Epub] CrossRef
Quantification by qPCR of Pathobionts in Chronic Periodontitis: Development of Predictive Models of Disease Severity at Site-Specific Level
Inmaculada Tomás, Alba Regueira-Iglesias, Maria López, Nora Arias-Bujanda, Lourdes Novoa, Carlos Balsa-Castro, Maria Tomás
Frontiers in Microbiology.2017;[Epub] CrossRef
Gingipain‐dependent augmentation by Porphyromonas gingivalis of phagocytosis of Tannerella forsythia
Y.‐J. Jung, H.‐K. Jun, B.‐K. Choi
Molecular Oral Microbiology.2016; 31(6): 457. CrossRef
Development of Filifactor alocis media for single- and co-cultivation with periodontopathogens
조인우, 이성훈
Oral Biology Research.2016; 40(4): 193. CrossRef
Antagonistic effect of peptidoglycan of Streptococcus sanguinis on lipopolysaccharide of major periodontal pathogens
Sung-Hoon Lee
Journal of Microbiology.2015; 53(8): 553. CrossRef
Polymicrobial Oral Infection with Four Periodontal Bacteria Orchestrates a Distinct Inflammatory Response and Atherosclerosis in ApoEnull Mice
Sasanka S. Chukkapalli, Irina M. Velsko, Mercedes F. Rivera-Kweh, Donghang Zheng, Alexandra R. Lucas, Lakshmyya Kesavalu, Salomon Amar
PLOS ONE.2015; 10(11): e0143291. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Reducing the Bioactivity of Tannerella forsythia Lipopolysaccharide by Porphyromonas gingivalis

J. Microbiol. 2014;52(8):702-708. Published online August 1, 2014

DOI: https://doi.org/10.1007/s12275-014-4324-5

Related articles